РОЗВИТОК ENTEROBACTER NIMIPRESSURALIS 32-3 У РИЗОСФЕРІ РОСЛИН ВИНОГРАДУ
It was shown that phosphorous mobilizing bacterium E. nimipressuralis 32-3 can take roots in the rhizosphere of grapes due to the artificially acquired resistance to antibiotics. The ability to progress into the root area of plants explains the positive effect of bacteria on the productivity of grap...
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| Date: | 2014 |
|---|---|
| Main Author: | |
| Format: | Article |
| Language: | English Ukrainian |
| Published: |
Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine
2014
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| Subjects: | |
| Online Access: | https://smic.in.ua/index.php/journal/article/view/153 |
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| Journal Title: | Agriciltural microbiology |
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Institution
Agriciltural microbiology| _version_ | 1871465611364663296 |
|---|---|
| author | Клименко, Н. М. |
| author_facet | Клименко, Н. М. |
| author_institution_txt_mv | [
{
"author": "Н. М. Клименко",
"institution": "Інститут агроекології і природокористування НААН"
}
] |
| author_sort | Клименко, Н. М. |
| baseUrl_str | https://smic.in.ua/index.php/journal/oai |
| collection | OJS |
| datestamp_date | 2026-07-22T10:10:43Z |
| description | It was shown that phosphorous mobilizing bacterium E. nimipressuralis 32-3 can take roots in the rhizosphere of grapes due to the artificially acquired resistance to antibiotics. The ability to progress into the root area of plants explains the positive effect of bacteria on the productivity of grapes. |
| doi_str_mv | 10.35868/1997-3004.20.12-14 |
| first_indexed | 2025-07-17T12:24:08Z |
| format | Article |
| fulltext |
DEVELOPMENT OF ENTEROBACTER NIMIPRESSURALIS 32-3
IN GRAPE PLANTS RHIZOSPHERE
N. M. Klymenko
It is well known that microbial
preparations used in the cultivation of various
crops have a number of useful properties.
First, they are environmentally safe for the
environment and human, secondly – they
have the ability to improve mineral nutrition
of plants, to improve their growth and
productivity. In addition, bacterial strains, that
make basis for biological preparations, have
positive impact on the formation of groups of
microorganisms in the soil [1; 2]. However,
the effectiveness of preparation use depends
on the ability of bacterial strains to survive in
the rhizosphere of plants.
One of the promising microbial
preparations based on phosphate mobilizing
bacteria is Phosphoenteryn. Research
conducted by a number of authors suggest
that strain-basis of this preparation
(Enterobacter nimipressuralis 32-3) is
capable to survive in the rhizosphere of corn
and vegetables [3-5]. However, the ability of
this bacterium to grow and develop in the root
areas of grape has not been studied yet. In this
context, the goal of our research was to study
the survival ability of E. nimipressuralis 32-3
in the rhizosphere of grape plants.
Materials and methods. The study
was conducted during growing experiment
with Muscat white at Chasselas x Berlandieri
41B rootstock. The duration of the experiment
was 70 days. The survival ability of E.
nimipressuralis 32-3 in the rhizosphere of
grape was studied through its streptomycin-,
ampicillin- and kanamycin-resistant mutants.
Antibiotic-resistant strain mutants were
obtained by Zibalskyi method [6]. The final
concentration of antibiotics in the culture
medium, that did not keep the studied bacteria
from growth, was 1000 units/ml of
streptomycin, 100 units/ml of ampicillin and
kanamycin.
Grape root system was treated with the
suspension made of diurnal culture of
microorganisms (10.2-11.5 million CFU/ml)
in the amount of 6 ml per each seedling.
Plants were planted in the vessels of 6 litres
each filled with meadow alluvial carbonated
soil. The main characteristics of the soil:
humus reserves 1.5-2.2%; mobile nitrogen
content 11-18 mg/kg of soil, phosphorus – 32-
38 mg/kg, potassium – 260-430 mg/kg;
reaction of soil solution (pH) 8.2-8.5;
carbonate content – 15-37%; active lime
content – 10-15%. These characteristics of
soil are optimized for growing grape. Six-time
repetition of the experiment.
The number of bacterial cells mutants
in the rhizosphere of the plant was determined
by deep planting of certain dilutions of soil
suspension onto the culture medium (glucose-
aspartic agar) supplemented with 1000
units/ml of streptomycin, 100 units/ml of
ampicillin and kanamycin, 5-time repetition.
The number of bacteria naturally resistant to
antibiotics is stated in the control. Soil
samples for this variant were taken in the
rhizosphere of non-bacterized plants.
Thus, the conducted studies show that
E. nimipressuralis 32-3 has the ability to
survive in the rhizosphere of grape and can
adapt and exist on the surface of grape roots.
These results explain the data that we
obtained during a field experiment, namely:
the use of Phosphoenteryn makes positive
impact on the growth, development and yield
of grape [7].
|
| id | oai:ojs2.smic.in.ua:article-153 |
| institution | Agriciltural microbiology |
| keywords_txt_mv | keywords |
| language | English Ukrainian |
| last_indexed | 2026-07-23T01:04:47Z |
| publishDate | 2014 |
| publisher | Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine |
| record_format | ojs |
| resource_txt_mv | smicinua/64/f66c7482ecbc8ca878c5a4edc83d8d64.pdf |
| spelling | oai:ojs2.smic.in.ua:article-1532026-07-22T10:10:43Z DEVELOPMENT OF ENTEROBACTER NIMIPRESSURALIS 32-3 IN GRAPE PLANTS RHIZOSPHERE РОЗВИТОК ENTEROBACTER NIMIPRESSURALIS 32-3 У РИЗОСФЕРІ РОСЛИН ВИНОГРАДУ Клименко, Н. М. E. nimipressuralis 32-3, grapes, rhizosphere, antibiotic-resistant bacteria E. nimipressuralis 32-3, виноград, ризосфера, антибіотико-резистентні бактерії It was shown that phosphorous mobilizing bacterium E. nimipressuralis 32-3 can take roots in the rhizosphere of grapes due to the artificially acquired resistance to antibiotics. The ability to progress into the root area of plants explains the positive effect of bacteria on the productivity of grapes. За використання штучно набутої антибіотикорезистентної ознаки встановлено, що фосфатмобілізувальна бактерія E. nimipressuralis 32-3 здатна приживатися в ризосфері рослин винограду. Здатність до розвитку в кореневих сферах рослин пояснює позитивний вплив бактерії на продуктивність винограду. Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine 2014-12-22 Article Article Рецензована Стаття application/pdf application/pdf https://smic.in.ua/index.php/journal/article/view/153 10.35868/1997-3004.20.12-14 Agricultural microbiology; Vol. 20 (2014): Agriciltural microbiology; 12-14 Сільськогосподарська мікробіологія; Том 20 (2014): Сільськогосподарська мікробіологія; 12-14 1997-3004 10.35868/1997-3004.20 en uk https://smic.in.ua/index.php/journal/article/view/153/191 https://smic.in.ua/index.php/journal/article/view/153/192 Авторське право (c) 2014 N. M. Klymenko https://creativecommons.org/licenses/by/4.0 |
| spellingShingle | E. nimipressuralis 32-3 виноград ризосфера антибіотико-резистентні бактерії Клименко, Н. М. РОЗВИТОК ENTEROBACTER NIMIPRESSURALIS 32-3 У РИЗОСФЕРІ РОСЛИН ВИНОГРАДУ |
| title | РОЗВИТОК ENTEROBACTER NIMIPRESSURALIS 32-3 У РИЗОСФЕРІ РОСЛИН ВИНОГРАДУ |
| title_alt | DEVELOPMENT OF ENTEROBACTER NIMIPRESSURALIS 32-3 IN GRAPE PLANTS RHIZOSPHERE |
| title_full | РОЗВИТОК ENTEROBACTER NIMIPRESSURALIS 32-3 У РИЗОСФЕРІ РОСЛИН ВИНОГРАДУ |
| title_fullStr | РОЗВИТОК ENTEROBACTER NIMIPRESSURALIS 32-3 У РИЗОСФЕРІ РОСЛИН ВИНОГРАДУ |
| title_full_unstemmed | РОЗВИТОК ENTEROBACTER NIMIPRESSURALIS 32-3 У РИЗОСФЕРІ РОСЛИН ВИНОГРАДУ |
| title_short | РОЗВИТОК ENTEROBACTER NIMIPRESSURALIS 32-3 У РИЗОСФЕРІ РОСЛИН ВИНОГРАДУ |
| title_sort | розвиток enterobacter nimipressuralis 32-3 у ризосфері рослин винограду |
| topic | E. nimipressuralis 32-3 виноград ризосфера антибіотико-резистентні бактерії |
| topic_facet | E. nimipressuralis 32-3 grapes rhizosphere antibiotic-resistant bacteria E. nimipressuralis 32-3 виноград ризосфера антибіотико-резистентні бактерії |
| url | https://smic.in.ua/index.php/journal/article/view/153 |
| work_keys_str_mv | AT klimenkonm developmentofenterobacternimipressuralis323ingrapeplantsrhizosphere AT klimenkonm rozvitokenterobacternimipressuralis323urizosferíroslinvinogradu |