ВИВЧЕННЯ КУЛЬТУРАЛЬНО-МОРФОЛОГІЧНИХ ОСОБЛИВОСТЕЙ ФІТОПАТОГЕННОГО ГРИБА ACREMONIUM SP. 502
The paper provides the description of cultural-morphological features of phytopathogenic fungus Acremonium sp. 502 belonging to the group of slow-growing bacteria. The highest radial growth rate was achieved on a potatoglucose agar (0.1375 ± 0.0011 mm/h); the optimum medium pH was 8.5; the optimum t...
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| Date: | 2014 |
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| Format: | Article |
| Language: | English Ukrainian |
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Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine
2014
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| Online Access: | https://smic.in.ua/index.php/journal/article/view/158 |
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| Journal Title: | Agriciltural microbiology |
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Agriciltural microbiology| _version_ | 1871465628473229312 |
|---|---|
| author | Цехмістер, Г. В. |
| author_facet | Цехмістер, Г. В. |
| author_institution_txt_mv | [
{
"author": "Г. В. Цехмістер",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
}
] |
| author_sort | Цехмістер, Г. В. |
| baseUrl_str | https://smic.in.ua/index.php/journal/oai |
| collection | OJS |
| datestamp_date | 2026-07-22T10:10:43Z |
| description | The paper provides the description of cultural-morphological features of phytopathogenic fungus Acremonium sp. 502 belonging to the group of slow-growing bacteria. The highest radial growth rate was achieved on a potatoglucose agar (0.1375 ± 0.0011 mm/h); the optimum medium pH was 8.5; the optimum temperature — 26 °C. |
| doi_str_mv | 10.35868/1997-3004.20.49-53 |
| first_indexed | 2025-07-17T12:24:10Z |
| format | Article |
| fulltext |
THE STUDY OF THE CULTURAL- MORPHOLOGICAL FEATURES OF
PHYTOPATHOGENIC FUNGUS ACREMONIUMSP. 502
G. V. Tsekhmister
Representatives of Cucurbitaceae
family are valuable crops and rank third by
acreage in Ukraine. During the growing
season they are affected by negative influence
of different phytopathogens that results in
considerable loss of farms. Representatives of
Acremonium genus can cause disease to
plants of Cucurbitaceae family [15]. The
disease was first described in 1994 in Spain
on melon plants [10]. The following year it
was recorded in Texas on melon and
watermelon plants [12] and in 1996 it was
established that the causative agent was
Acremonium cucurbitacearum [18]. It is also
know that the representative of Acremonium
genus caused the similar disease of melons
observed in California [11]. In 2002 in Italy
A. cucurbitacearum, A. crotonigenum and A.
Sclerotigenum were also isolated from
collapsed melons [16]. It is also know that A.
sclerotigenum Gams, which has a saprophytic
type of nutrition, under certain conditions
(high concentration of salt in soil) can act as
vascular parasite of cucumber plants [7].
There are no data about the spread of
the disease in Ukraine. In 2011
S. P. Nadkernychnyi isolated Acremonium
sp. 502 strain from sick cucumber plants that
were grown in the conditions of covered soil.
We have confirmed its pathogenicity to
cucumber plants [8].
The development of pathogenic fungi
largely depends on the conditions created by
biotic and abiotic factors. The main abiotic
factor affecting the spread and development
of cucumber diseases is temperature. The
activity of each kind life activity is possible
only in certain temperature ranges.
Temperature optimum in most is the
determining factor for the dominance of a
particular type in agrocenoses. For a long
period the pathogen can exist without
showing activity, and only at optimal
temperature it will manifest its parasitic effect
[4]. For example, potato phytopathogen
Phytophthora infestans develops within the
range of 2-26°C, with an optimum at 18-
21°C. It is known that temperature optimum
for corn cob white rot causative agent
Fusarium poae is 24-26°C [5].
In many cases, the lesion of
Cucurbitaceae family with diseases is
associated with abiotic environmental
conditions, especially temperature [9; 13; 14].
In 1982 W. D. Gubler [17] noted that the
disease of seedlings of that family caused by
A. cucurbitacearum was lower at 17°C and
much higher at 27°C, with an optimum at
24°C.
Another equally important factor is the
pH of the medium in which the fungus
develops. For each strain of pathogenic
microorganisms there are certain optimal
limits of pH. This factor not only influences
the growth and development of the fungus,
but also its ability to produce different
metabolites that affect the plant [1]. Many
fungi are tolerant to a wide range of medium
pH. Thus, it is known that Pénicillium
variabelis, Fusarium bullatum and F.
oxysporium can grow within 2.0-11.0 pH
limits [3].
Taking into account the above, the
goal of our work was to study certain cultural-
morphological features of Acremonium
sp. 502 at different culture conditions.
Materials and methods. The paper
used pathogenic for cucumbers strain of fungi
of Acremonium genus, isolated from sick
cucumber plants grown in the conditions of
covered soil. Strain virulence was previously
confirmed on cucumbers of Koroliok variety.
Fungus culture was kept on slant wort-agar (4
Balling degrees).
The research of cultural-
morphological features of Acremonium
sp. 502 was studied on wort-agar (WA),
agarized Chapek synthetic medium (CA) and
potato-glucose agar (PGA). Media were
poured into Petri dishes. Incubated at 26±2°C.
On the 10th day radial growth rate of fungus
colonies was noted. The description of
colonies was performed on the day 28, after
the complete overgrowth of culture medium
in a Petri dish by fungus mycelium. The
colour of the colonies, their shape, surface
type, thallus type, mycelium septate, view of
the colony on the liquid medium,
reproduction features, conidia and
conidiophores size, sporification intensity.
The determination of temperature
range of growth for Acremonium sp. 502 was
performed using wort-agar. First, fungus was
cultured at the temperature of 26±2°C within
one day to initiate growth. The following day
fungus cultivation continued at different
temperature regimes: 8°C, 10°C, 18°C, 26°C
and 34°C (±2°C). On the 10th day colonies
diameter was fixed in two mutually
perpendicular directions and radial growth
rate was determined by methods [6].
The research of optimum pH was
determined by the diameter of fungus colony
growth on wort-agar (pH 3.5; 4.5; 5.0; 6.0;
6.5; 7.0; 7.5; 8.0; 8.5; 9.0; 9.5; 10.0; 10.5;
11.0; 11.5; 12.0). Cultivation was carried out
at the temperature of 26±2°C. On the day 10
colonies diameter was fixed in two mutually
perpendicular direct.
Repetition of the experiment
quadruple. Statistical analysis of the results
was performed by standard methods [2] using
Microsoft Excel applications. At processing
of experimental data mean-square deviation
was calculated.
So, the analysis of the results of the
study indicates the variability in Acremonium
sp. 502 growth depending on the composition
of nutrition medium, temperature rate and
medium pH. By growth characteristic the
fungus can be attributed to the group of slow-
growing. Differences in the size of conidia
were fixed when grown in different media. It
was established that the optimum temperature
for Acremonium sp. 502 growth is 26°C,
optimal medium reaction for the development
of the fungus – slightly alkaline (pH 8.5).
|
| id | oai:ojs2.smic.in.ua:article-158 |
| institution | Agriciltural microbiology |
| keywords_txt_mv | keywords |
| language | English Ukrainian |
| last_indexed | 2026-07-23T01:05:03Z |
| publishDate | 2014 |
| publisher | Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine |
| record_format | ojs |
| resource_txt_mv | smicinua/e9/708b4b1ae574e7c5f2ee435e17507ce9.pdf |
| spelling | oai:ojs2.smic.in.ua:article-1582026-07-22T10:10:43Z THE STUDY OF THE CULTURAL- MORPHOLOGICAL FEATURES OF PHYTOPATHOGENIC FUNGUS ACREMONIUMSP. 502 ВИВЧЕННЯ КУЛЬТУРАЛЬНО-МОРФОЛОГІЧНИХ ОСОБЛИВОСТЕЙ ФІТОПАТОГЕННОГО ГРИБА ACREMONIUM SP. 502 Цехмістер, Г. В. phytopathogenic fungus, Acremonium, cultural-morphological features фітопатоген, Acremonium, культурально-морфологічні особливості The paper provides the description of cultural-morphological features of phytopathogenic fungus Acremonium sp. 502 belonging to the group of slow-growing bacteria. The highest radial growth rate was achieved on a potatoglucose agar (0.1375 ± 0.0011 mm/h); the optimum medium pH was 8.5; the optimum temperature — 26 °C. Описано культурально-морфологічні особливості фітопатогенного гриба Acremonium sp. 502. Гриб віднесено до групи повільнорослих. Найвища радіальна швидкість росту на картопляно-глюкозному агарі становила 0,1375 ± 0,0011 мм/год., оптимальний показник рН середовища — 8,5, оптимальна температура — 26 °С. Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine 2014-12-22 Article Article Рецензована Стаття application/pdf application/pdf https://smic.in.ua/index.php/journal/article/view/158 10.35868/1997-3004.20.49-53 Agricultural microbiology; Vol. 20 (2014): Agriciltural microbiology; 49-53 Сільськогосподарська мікробіологія; Том 20 (2014): Сільськогосподарська мікробіологія; 49-53 1997-3004 10.35868/1997-3004.20 en uk https://smic.in.ua/index.php/journal/article/view/158/201 https://smic.in.ua/index.php/journal/article/view/158/202 Авторське право (c) 2014 G. V. Tsekhmister https://creativecommons.org/licenses/by/4.0 |
| spellingShingle | фітопатоген Acremonium культурально-морфологічні особливості Цехмістер, Г. В. ВИВЧЕННЯ КУЛЬТУРАЛЬНО-МОРФОЛОГІЧНИХ ОСОБЛИВОСТЕЙ ФІТОПАТОГЕННОГО ГРИБА ACREMONIUM SP. 502 |
| title | ВИВЧЕННЯ КУЛЬТУРАЛЬНО-МОРФОЛОГІЧНИХ ОСОБЛИВОСТЕЙ ФІТОПАТОГЕННОГО ГРИБА ACREMONIUM SP. 502 |
| title_alt | THE STUDY OF THE CULTURAL- MORPHOLOGICAL FEATURES OF PHYTOPATHOGENIC FUNGUS ACREMONIUMSP. 502 |
| title_full | ВИВЧЕННЯ КУЛЬТУРАЛЬНО-МОРФОЛОГІЧНИХ ОСОБЛИВОСТЕЙ ФІТОПАТОГЕННОГО ГРИБА ACREMONIUM SP. 502 |
| title_fullStr | ВИВЧЕННЯ КУЛЬТУРАЛЬНО-МОРФОЛОГІЧНИХ ОСОБЛИВОСТЕЙ ФІТОПАТОГЕННОГО ГРИБА ACREMONIUM SP. 502 |
| title_full_unstemmed | ВИВЧЕННЯ КУЛЬТУРАЛЬНО-МОРФОЛОГІЧНИХ ОСОБЛИВОСТЕЙ ФІТОПАТОГЕННОГО ГРИБА ACREMONIUM SP. 502 |
| title_short | ВИВЧЕННЯ КУЛЬТУРАЛЬНО-МОРФОЛОГІЧНИХ ОСОБЛИВОСТЕЙ ФІТОПАТОГЕННОГО ГРИБА ACREMONIUM SP. 502 |
| title_sort | вивчення культурально-морфологічних особливостей фітопатогенного гриба acremonium sp. 502 |
| topic | фітопатоген Acremonium культурально-морфологічні особливості |
| topic_facet | phytopathogenic fungus Acremonium cultural-morphological features фітопатоген Acremonium культурально-морфологічні особливості |
| url | https://smic.in.ua/index.php/journal/article/view/158 |
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