ОСОБЛИВОСТІ РОЗВИТКУ ВІРУСНОЇ ІНФЕКЦІЇ, СПРИЧИНЕНОЇ POTATO VIRUS M, НА РОСЛИНАХ КАРТОПЛІ ЗА ДІЇ МІКРОБНИХ ПРЕПАРАТІВ
The paper presents the results of developmental study of viral infection caused by Potato virus M under the impact of microbial preparations on potato plants. Use of biopreparations Biogran and Bactopaslen, at artificial plants infection with PVM had ensured development of higher plants infected wit...
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| Datum: | 2014 |
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| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | Englisch Ukrainisch |
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Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine
2014
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| Online Zugang: | https://smic.in.ua/index.php/journal/article/view/160 |
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| Назва журналу: | Agriciltural microbiology |
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Agriciltural microbiology| _version_ | 1871465630138368000 |
|---|---|
| author | Будзанівська, І. Г. Бова, Т. О. Кучерявенко, О. О. Пиріг, О. В. Дмитрук, О. О. |
| author_facet | Будзанівська, І. Г. Бова, Т. О. Кучерявенко, О. О. Пиріг, О. В. Дмитрук, О. О. |
| author_institution_txt_mv | [
{
"author": "І. Г. Будзанівська",
"institution": "Київський національний університет ім. Тараса Шевченка, ННЦ «Інститут біології»"
},
{
"author": "Т. О. Бова",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "О. О. Кучерявенко",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "О. В. Пиріг",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "О. О. Дмитрук",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
}
] |
| author_sort | Будзанівська, І. Г. |
| baseUrl_str | https://smic.in.ua/index.php/journal/oai |
| collection | OJS |
| datestamp_date | 2026-07-22T10:10:43Z |
| description | The paper presents the results of developmental study of viral infection caused by Potato virus M under the impact of microbial preparations on potato plants. Use of biopreparations Biogran and Bactopaslen, at artificial plants infection with PVM had ensured development of higher plants infected with virus, increase of total chlorophyll content compared to control, decrease of virus concentration in plants and activity of ribonuclease enzyme in potato plants infected with PVM. |
| doi_str_mv | 10.35868/1997-3004.20.60-65 |
| first_indexed | 2025-07-17T12:24:11Z |
| format | Article |
| fulltext |
CHARACTERISTICS OF VIRAL INFECTION DEVELOPMENT CAUSED
BY POTATO VIRUS M UNDER THE IMPACT OF BIOPREPARATIONS ON
POTATO PLANTS
I. H. Budzanivska1, T. O. Bova2, O. O. Kucheriavenko2, O. V. Pyrih2,
O. O. Dmytruk2
1Taras Shevchenko National University of Kyiv, ESC «Institute of Biology»,
2, Hlushkova Avenue, Kyiv City, 03187, Ukraine; е-mail: decanat_bf@univ.kiev.ua
Viral diseases are the cause of potato
varieties degeneration, and they are the
subject of close attention for potato breeders
in many countries [1]. The reproduction of the
virus in the plant causes significant
physiological changes and functional
disorders (metabolic imbalances of protein,
nucleic acids, carbohydrates, photosynthesis,
respiration, transpiration and water exchange,
etc.) [2].
Potato virus M (PVM, Potato virus M,
Carlavirus genus, Betaflexiviridae family) is
one of the most common pathogens of potato
in all areas of cultivation. Yield losses from
M-viral infection can reach 41%. Long-term
data of the virology laboratory staff of the
Institute of Agricultural Microbiology and
Agroindustrial Manufacture of NAAS
(IAMAM NAAS) testify that PVM is
manifested as in monoinfection so in
combination with other mosaic viruses. In
addition, in 20-70% cases asymptomatic viral
disease was detected. That is, even at the
stage of growing of seeding sets the
preconditions for economic loss in potato
farming are formed [3; 4]. Therefore, finding
the ways to reduce the loss from viral
infections in potato crops is urgent nowadays.
The use of physiologically active
substances that can stimulate natural defence
mechanisms of plants is considered as a
promising strategy in protecting against viral
infections. Microbial preparations designed to
improve nitrogen and phosphorus nutrition of
plants distinguish with comprehensive
positive impact on plants and safety for the
environment and are one of the components
of modern technologies of crops planting [5].
It should be noted that among the
crops for which bacterial preparations were
developed potato is somewhat aloof. The
studies on potato inoculation are not as
systematic and large-scale, as for many other
crops, in Ukraine this item is also neglected
[6].
Potato belongs to the crops with very
poorly developed root system [6; 7]. This
gives reason to state that bacterization can
play a special role in the technology of
cultivation of this crop, since influencing the
course of microbiological processes in the
rhizosphere of plants their provision with
nutrition elements can be improved, which, in
turn, will stimulate positive changes in the
growth and development of plants, yield
formation and also provide improvement in
the quality of obtained products [7].
In this context, the goal of this work
was to study the characteristics of viral
infection development caused by Potato virus
M under the impact of biopreparations on
potato plants.
Materials and methods. The studies
used a collection strain of PVM, which was
isolated from potato plants of Nahoroda
(Award) variety in the virology laboratory of
IAMAM NAAS. The accumulation of the
virus was carried out on Lycopersicon
esculentum Mill. plants that were infected in
the phase of 3-4 true leaves by mechanical
inoculation [8; 9]. 21 days after infecting
tomato plants were tested for the presence of
PVM through the methods of electron
microscopy [10] and polymerase chain
reaction (PCR) [11].
Potato plants in vitro of Zhukovskyi
early variety were used in the study, and they
were planted in sterile soil. Soil moisture was
maintained at 60% from full capacity during
the growing season.
The treatment of potato plant roots
was done in the day of planting. Root system
was soaked Biogran and Bactopaslen at the
rate of 1:10 and 1:100 respectively. Reference
samples were treated with water. Repetition
of the experiment – 15-time.
14 days after planting of meristem
potato into the soil part of the plants was
infected with Potato virus M. The presence of
virus in the experimental samples of potato
plants was controlled on the 21st day after the
lesion with PVM by the use of electron
microscopy [10] and enzyme immunoassay
(EIA) [12]. Healthy tobacco plants (for
negative control) and plants with PVM (for
positive control) were used as the control.
The definition RNAase activity was
performed on the 22nd day after the lesion by
A. K. Chakravorty method modified by
Yu. I. Vlasov [13].
Sample weight of 1 kg of fresh plant
material was triturated with 15 ml of 0.06 M
phosphate buffer, pH 6.7. The trituration was
performed on ice using silica sand. Then plant
mass was pressed through 2 layers of gauze.
The resulting extract was centrifuged at
10,000 g for 25 min. The precipitate was
discarded, and the supernatant was
immediately acidified with 1 N hydrochloric
acid to pH 5.0 and left overnight at the
temperature of 0°C. Sediment dropped which
were released by centrifugation at 4000 g for
25 min., and the clear solution was used to
determine the activity of RNAase. For this
enzyme extract was adjusted to 20 ml of
phosphate buffer.
The studied sample that consisted of
0.7 ml of enzyme extract, 1 ml of phosphate
buffer pH 5.0 and 0.3 ml of substrate
(standard solution – yeast RNAase, 10 mg/ml)
was incubated in centrifuge tubes in the
incubator at the temperature of 37°C during
75 min. The procedure of making the reaction
mixture was carried out in an ice bath. After
the incubation 2 ml of precipitator (mixture of
50 ml 96% ethanol with 1.7 ml 57% NClO4)
were added to the reaction mixture. This
operation was also performed on ice.
Reference samples were prepared to each
studied sample. In reference samples the
precipitator was added before the enzyme
extract to stop the reaction.
The samples were immediately
transferred on the ice bath after the incubation
and kept in the cold for 2 hours to complete
precipitation. Then centrifugation was
performed at 3000 g for 30 min. The resulting
supernatant was adjusted to 10 ml of
phosphate buffer and spectrophotometered at
260 nm.
So, biological preparations Biogran
and Bactopaslen improved the physiological
state of the potato plants of Zhukovskyi early
variety at artificial plants infection with PVM.
The use of biopreparations ensured
development of higher infected plants
compared to control by 6.0-15.5%; total
chlorophyll content – by 14.0% and 3.3%
respectively. At the action of biopreparations
the decrease of Potato virus M concentration
in plants was noted, and, consequently, the
decrease activity of ribonuclease.
|
| id | oai:ojs2.smic.in.ua:article-160 |
| institution | Agriciltural microbiology |
| keywords_txt_mv | keywords |
| language | English Ukrainian |
| last_indexed | 2026-07-23T01:05:05Z |
| publishDate | 2014 |
| publisher | Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine |
| record_format | ojs |
| resource_txt_mv | smicinua/43/ce5d42d7459c9e3de40dc2a24a39b443.pdf |
| spelling | oai:ojs2.smic.in.ua:article-1602026-07-22T10:10:43Z CHARACTERISTICS OF VIRAL INFECTION DEVELOPMENT CAUSED BY POTATO VIRUS M UNDER THE IMPACT OF BIOPREPARATIONS ON POTATO PLANTS ОСОБЛИВОСТІ РОЗВИТКУ ВІРУСНОЇ ІНФЕКЦІЇ, СПРИЧИНЕНОЇ POTATO VIRUS M, НА РОСЛИНАХ КАРТОПЛІ ЗА ДІЇ МІКРОБНИХ ПРЕПАРАТІВ Будзанівська, І. Г. Бова, Т. О. Кучерявенко, О. О. Пиріг, О. В. Дмитрук, О. О. potato, viral infection, ribonuclease, microbial preparations картопля, вірусна інфекція, рибонуклеаза, мікробні препарати The paper presents the results of developmental study of viral infection caused by Potato virus M under the impact of microbial preparations on potato plants. Use of biopreparations Biogran and Bactopaslen, at artificial plants infection with PVM had ensured development of higher plants infected with virus, increase of total chlorophyll content compared to control, decrease of virus concentration in plants and activity of ribonuclease enzyme in potato plants infected with PVM. Досліджено особливості розвитку вірусної інфекції, спричиненої Potato virus M, на рослинах картоплі за дії мікробних препаратів. Застосування біопрепаратів Біограну та Бактопасльону за умов штучного інфікування МВК сприяло збільшенню висоти уражених рослин та сумарного вмісту хлорофілу відносно контролю, зниженню концентрації вірусу в рослинах та активності ферменту рибонуклеази в інфікованих МВК рослинах картоплі. Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine 2014-12-22 Article Article Рецензована Стаття application/pdf application/pdf https://smic.in.ua/index.php/journal/article/view/160 10.35868/1997-3004.20.60-65 Agricultural microbiology; Vol. 20 (2014): Agriciltural microbiology; 60-65 Сільськогосподарська мікробіологія; Том 20 (2014): Сільськогосподарська мікробіологія; 60-65 1997-3004 10.35868/1997-3004.20 en uk https://smic.in.ua/index.php/journal/article/view/160/205 https://smic.in.ua/index.php/journal/article/view/160/206 Авторське право (c) 2014 I. H. Budzanivska, T. O. Bova, O. O. Kucheriavenko, O. V. Pyrih, O. O. Dmytruk https://creativecommons.org/licenses/by/4.0 |
| spellingShingle | картопля вірусна інфекція рибонуклеаза мікробні препарати Будзанівська, І. Г. Бова, Т. О. Кучерявенко, О. О. Пиріг, О. В. Дмитрук, О. О. ОСОБЛИВОСТІ РОЗВИТКУ ВІРУСНОЇ ІНФЕКЦІЇ, СПРИЧИНЕНОЇ POTATO VIRUS M, НА РОСЛИНАХ КАРТОПЛІ ЗА ДІЇ МІКРОБНИХ ПРЕПАРАТІВ |
| title | ОСОБЛИВОСТІ РОЗВИТКУ ВІРУСНОЇ ІНФЕКЦІЇ, СПРИЧИНЕНОЇ POTATO VIRUS M, НА РОСЛИНАХ КАРТОПЛІ ЗА ДІЇ МІКРОБНИХ ПРЕПАРАТІВ |
| title_alt | CHARACTERISTICS OF VIRAL INFECTION DEVELOPMENT CAUSED BY POTATO VIRUS M UNDER THE IMPACT OF BIOPREPARATIONS ON POTATO PLANTS |
| title_full | ОСОБЛИВОСТІ РОЗВИТКУ ВІРУСНОЇ ІНФЕКЦІЇ, СПРИЧИНЕНОЇ POTATO VIRUS M, НА РОСЛИНАХ КАРТОПЛІ ЗА ДІЇ МІКРОБНИХ ПРЕПАРАТІВ |
| title_fullStr | ОСОБЛИВОСТІ РОЗВИТКУ ВІРУСНОЇ ІНФЕКЦІЇ, СПРИЧИНЕНОЇ POTATO VIRUS M, НА РОСЛИНАХ КАРТОПЛІ ЗА ДІЇ МІКРОБНИХ ПРЕПАРАТІВ |
| title_full_unstemmed | ОСОБЛИВОСТІ РОЗВИТКУ ВІРУСНОЇ ІНФЕКЦІЇ, СПРИЧИНЕНОЇ POTATO VIRUS M, НА РОСЛИНАХ КАРТОПЛІ ЗА ДІЇ МІКРОБНИХ ПРЕПАРАТІВ |
| title_short | ОСОБЛИВОСТІ РОЗВИТКУ ВІРУСНОЇ ІНФЕКЦІЇ, СПРИЧИНЕНОЇ POTATO VIRUS M, НА РОСЛИНАХ КАРТОПЛІ ЗА ДІЇ МІКРОБНИХ ПРЕПАРАТІВ |
| title_sort | особливості розвитку вірусної інфекції, спричиненої potato virus m, на рослинах картоплі за дії мікробних препаратів |
| topic | картопля вірусна інфекція рибонуклеаза мікробні препарати |
| topic_facet | potato viral infection ribonuclease microbial preparations картопля вірусна інфекція рибонуклеаза мікробні препарати |
| url | https://smic.in.ua/index.php/journal/article/view/160 |
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