НАПРАВЛЕНІСТЬ БІОЛОГІЧНИХ ПРОЦЕСІВ У КОРЕНЕВІЙ ЗОНІ РОСЛИН КАРТОПЛІ ЗА ДІЇ ДОБРИВ ТА БІОПРЕПАРАТУ
The paper covers the results of the orientation of different biological processes (nitrogen fixation, N2O and CO2 emissions) in the root zone of potato plants under the different fertilization systems in a field experiment in stationary experiment on leached black soils. The application of 40 t/ha o...
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| Datum: | 2014 |
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| Sprache: | Englisch Ukrainisch |
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Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine
2014
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| Назва журналу: | Agriciltural microbiology |
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Agriciltural microbiology| _version_ | 1871465634642001920 |
|---|---|
| author | Волкогон, В. В. Журба, М. А. Дімова, С. Б. Токмакова, Л. М. Волкогон, К. І. Проценко, О. І. |
| author_facet | Волкогон, В. В. Журба, М. А. Дімова, С. Б. Токмакова, Л. М. Волкогон, К. І. Проценко, О. І. |
| author_institution_txt_mv | [
{
"author": "В. В. Волкогон",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "М. А. Журба",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "С. Б. Дімова",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "Л. М. Токмакова",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "К. І. Волкогон",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "О. І. Проценко",
"institution": "Чернігівська філія ДУ «Інститут охорони ґрунтів України»"
}
] |
| author_sort | Волкогон, В. В. |
| baseUrl_str | https://smic.in.ua/index.php/journal/oai |
| collection | OJS |
| datestamp_date | 2026-07-22T10:10:43Z |
| description | The paper covers the results of the orientation of different biological processes (nitrogen fixation, N2O and CO2 emissions) in the root zone of potato plants under the different fertilization systems in a field experiment in stationary experiment on leached black soils. The application of 40 t/ha of cattle manure was shown to be more environmentally beneficial (although with some caution, due to the high level of N2O emissions). The N40P40K40 dose of fertilizers was shown to be optimal, while the balance between environmental requirements and crop productivity shows the practicability of application of N80P80K80. High doses of fertilizers (N120P120K120) and organic mineral fertilization (40 t/ha manure + N80P80K80) had resulted in high levels of N2O and CO2 emissions and reduced nitrogen fixation activity. Application of biological preparation Biogran was shown to be efficient in the potato growing technology due to its proved ability to optimize the ecological condition of soils in agrocoenoses, increase productivity and improve the quality of potatoes. |
| doi_str_mv | 10.35868/1997-3004.19.3-10 |
| first_indexed | 2025-07-17T12:24:12Z |
| format | Article |
| fulltext |
ORIENTATION OF BIOLOGICAL PROCESSES IN THE ROOT ZONE OF
POTATO PLANTS UNDER THE INFLUENCE OF FERTILIZERS AND
BIOLOGICAL PREPARATION
V. V. Volkohon1, M. A. Zhurba1, S. B. Dimova1, L. M. Tokmakova1,
K. I. Volkohon1, O. I. Protsenko2
2Chernihiv branch of State Institution «Soil Protection Institute of Ukraine»
41, Malynovskoho Str., Chernihiv, 14020, Ukraine
The existing systems of crops
fertilization typically do not account for the
environmental protection requirements, as
required doses of fertilizers are calculated by
the nutrients removal rates (NPK) with
planned yield, and the degree of use of
fertilizers active ingredient is rather low.
Thus, according to the averaged data the rates
of nitrogen fixation are within 0.35-0.50,
phosphorus – not more than 0.2%, potassium
– 0.25-0.60 depending on soil type. [1] The
methods for calculating the required amount
of fertilizers would be ideal if fertilizer
absorption were at the rate of 100%. Since it
is impossible, the use of such calculations
leads to deliberate pollution with unused
fertilizers. In this regard special concern is
about mineral nitrogen as the biggest polluter
of agrocenoses.
Theoretically, nitrogen fertilizers
should be applied within the physiological
relevance. Only under this condition we can
minimize the risk of pollution with dangerous
to human life nitrogen compounds. But the
criterion of truth – what amount of mineral
nitrogen can be considered physiologically
appropriate for plants – is unknown. Such
opportunity appeared with the development of
biological testing methods, including the use
of indicators of functional activity of nitrogen
fixation bacteria associated with the roots of
crops. Since nitrogen fixers are able to fix
nitrogen from the atmosphere only in the
absence of excessive amounts of nitrogen
compounds in the environment, and as they
are closely connected with the roots of plants,
by the determination of nitrogenase activity of
the bacteria of the root zone of plants grown
in different agricultural backgrounds, we can
take choose such a dose that will not reduce
the activity of the process compared to the
control (without nitrogen) variant. On the
other hand, we know that diazotrophs stop
fixing nitrogen from the atmosphere in case of
nitrogen fertilizers excess and start using its
bound forms, since it is energetically more
favourable for bacteria cells. Thus, in addition
to nitrogen fixation for constructive
metabolism, bacteria carry out denitrification
process. So, having followed N2O emission
activity at different doses of mineral nitrogen
we can select the one that does not cause
significant losses of gaseous nitrogen from
the soil. Comparing the peculiarities of the
process of nitrogen fixation and
denitrification in agrocenoses, we can select
optimal dose of mineral nitrogen in
physiological aspect [2].
An additional test for the feasibility of
crops fertilization is the determination of the
intensity of mineralization processes in
agrocenoses soils, incl. the indicators of
carbon dioxide emission.
In connection with the above, the goal
of the study was to determine the orientation
of biological processes in the root zone of
potato plants under the effect of different
fertilization systems.
Materials and methods. The study
was conducted during 2011-2013 in stationary
field experiments on leached black soil (pHsal.
5.2; humus content – 3.01%, easily hydrolized
nitrogen – 109 mg/kg, mobile forms of
phosphate (P2O5) – 168 mg/kg, exchangeable
potassium (K2O) – 58 mg/kg of soil) of the
Institute of Agricultural Microbiology and
Agroindustrial Manufacture of NAAS. Potato
of Bellaroza variety was grown in rotation:
potato – spring barley – pea – winter wheat.
We determined the feasibility of such
fertilization systems: organic, low mineral,
medium mineral, high mineral and organic-
mineral.
Under organic system of fertilization
in crop rotation the manure was applied at the
rate of 40 t/ha once per rotation during
autumn ploughing (for potato). Mineral
fertilization of potato involved the use of
N40P40K40, N80P80K80 and Na120P120K120.
Organo-mineral system included the
application of 40 t/ha of manure and mineral
fertilizers (N80P80K80).
The experiment scheme also included
a block of variants, where microbial
preparation Biogran (TU 24.1-00497360-006:
2009) was used at the aforementioned
agricultural backgrounds.
The area of experimental plot – 86.4
m2 (7.2 × 12.0 m), the repetition of the
experiment is quadruple. The location of plots
is randomized.
The potential activity of nitrogen
fixation of potato plants in rhizosphere soil
was determined by M. Umarov [3]. To
evaluate the emission of nitrogen oxide in the
system “soil-plant” the method of closed
chambers was used [4; 5]. The 10 litres
chambers with rubber membrane for gases
separation were punched into the soil to the
depth of 10 cm, the soil around the chambers
was humidified to create a water gate.
Weighing bottles with calcium carbide
moistened with water to produce acetylene,
which inhibits nitric oxide reductase and
constrains the process of NO3ˉ NO2ˉ
dissimilation on at the stage of nitric oxide
restoration, were placed into the chambers to
determine N2O emission intensity. The
exposure was performed for three hours after
which gas samples were taken and placed into
previously evacuated vessels. The repetition
of sampling is three-time.
The selected gas samples were
analyzed by gas chromatograph. Nitrogenase
activity was determined by gas
chromatograph “Chrom-4” with a flame
ionization detector (steel column of 3 m
length filled with sorbent Parapak Q 60-80
mesh; thermostat temperature 40°C, gas flow:
hydrogen – 15 cm3/min., nitrogen – 1 0
cm3/min., and air – 500 cm3/min.).
Direct N2O emission was measured by
gas chromatograph “Tsvet 500 M” with the
detector of electrons capture. Columns
temperature – 40°C, evaporator temperature –
120°C, detector – 330°C. The losses of carrier
gas (argon with methane 95/5) – 35 cm3/min.
Sorption steel column 3 m high filled with
sorbent Parapak Q 60-80 mesh.
CO2 concentration was measured by
gas chromatograph “Tsvet 500 M” with
thermal conductivity detector (bridge current
130 mA). Temperature of columns 25°C,
detector – 40°C. The losses of carrier gas
(helium) – 20 cm3/min. [3]. Sorption steel
columns were filled with sorbent Parapak Q
60-80 mesh.
The experiment performance, crop
record and statistical analysis of the results
were carried out by B. Dospiekhov [6]. The
content of starch, nitrates and ascorbic acid
were determined according to the methods
[7].
Thus, according to the obtained
results, the lowest dose of mineral fertilizers
is optimal for environmental positions, but the
compromise between environmental
feasibility and culture performance suggests
in favour of promising choice of N80P80K80. It
makes sense (though with some reservations)
to apply 40 t/ha of manure under potato.
To optimize the ecological state of
agrocenoses, to increase potato productivity
and to improve products quality the use of
microbial preparation Biogran is promising in
the technologies of growing crops.
|
| id | oai:ojs2.smic.in.ua:article-164 |
| institution | Agriciltural microbiology |
| keywords_txt_mv | keywords |
| language | English Ukrainian |
| last_indexed | 2026-07-23T01:05:09Z |
| publishDate | 2014 |
| publisher | Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine |
| record_format | ojs |
| resource_txt_mv | smicinua/d7/2fa65246bc43b29f4f8d67c7336dcdd7.pdf |
| spelling | oai:ojs2.smic.in.ua:article-1642026-07-22T10:10:43Z ORIENTATION OF BIOLOGICAL PROCESSES IN THE ROOT ZONE OF POTATO PLANTS UNDER THE INFLUENCE OF FERTILIZERS AND BIOLOGICAL PREPARATION НАПРАВЛЕНІСТЬ БІОЛОГІЧНИХ ПРОЦЕСІВ У КОРЕНЕВІЙ ЗОНІ РОСЛИН КАРТОПЛІ ЗА ДІЇ ДОБРИВ ТА БІОПРЕПАРАТУ Волкогон, В. В. Журба, М. А. Дімова, С. Б. Токмакова, Л. М. Волкогон, К. І. Проценко, О. І. microbiological processes, fertilizers, nitrogen fixation, N2O emission, CO2 emission, potatoes мікробіологічні процеси, добрива, азотфіксація, емісія N2O і CO2, картопля The paper covers the results of the orientation of different biological processes (nitrogen fixation, N2O and CO2 emissions) in the root zone of potato plants under the different fertilization systems in a field experiment in stationary experiment on leached black soils. The application of 40 t/ha of cattle manure was shown to be more environmentally beneficial (although with some caution, due to the high level of N2O emissions). The N40P40K40 dose of fertilizers was shown to be optimal, while the balance between environmental requirements and crop productivity shows the practicability of application of N80P80K80. High doses of fertilizers (N120P120K120) and organic mineral fertilization (40 t/ha manure + N80P80K80) had resulted in high levels of N2O and CO2 emissions and reduced nitrogen fixation activity. Application of biological preparation Biogran was shown to be efficient in the potato growing technology due to its proved ability to optimize the ecological condition of soils in agrocoenoses, increase productivity and improve the quality of potatoes. У польовому стаціонарному досліді на чорноземі вилугуваному досліджено направленість біологічних процесів (азотфіксації, емісії N2O та СО2) у кореневій зоні рослин картоплі за різних систем удобрення. Екологічно сприятливим (хоча і з певним застереженням, зважаючи на високий рівень емісії N2O) є застосування 40 т/га гною ВРХ; доза мінеральних добрив N40P40K40 є оптимальною, проте компроміс між екологічними вимогами і продуктивністю культури свідчить про доцільність внесення N80P80K80. Високі дози мінеральних добрив (N120P120K120), а також органо-мінеральне удобрення (40 т/га гною + N80P80K80) призводять до високих рівнів емісії N2O і CO2 та зводять до мінімуму активність азотфіксації. З метою оптимізації екологічного стану ґрунтів агроценозів, підвищення продуктивності картоплі та покращення якості продукції в технологіях вирощування культури доцільне використання мікробного препарату Біограну. Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine 2014-06-26 Article Article Рецензована Стаття application/pdf application/pdf https://smic.in.ua/index.php/journal/article/view/164 10.35868/1997-3004.19.3-10 Agricultural microbiology; Vol. 19 (2014): Agriciltural microbiology; 3-10 Сільськогосподарська мікробіологія; Том 19 (2014): Сільськогосподарська мікробіологія; 3-10 1997-3004 10.35868/1997-3004.19 en uk https://smic.in.ua/index.php/journal/article/view/164/211 https://smic.in.ua/index.php/journal/article/view/164/212 Авторське право (c) 2014 V. V. Volkohon, M. A. Zhurba, S. B. Dimova, L. M. Tokmakova, K. I. Volkohon, O. I. Protsenko https://creativecommons.org/licenses/by/4.0 |
| spellingShingle | мікробіологічні процеси добрива азотфіксація емісія N2O і CO2 картопля Волкогон, В. В. Журба, М. А. Дімова, С. Б. Токмакова, Л. М. Волкогон, К. І. Проценко, О. І. НАПРАВЛЕНІСТЬ БІОЛОГІЧНИХ ПРОЦЕСІВ У КОРЕНЕВІЙ ЗОНІ РОСЛИН КАРТОПЛІ ЗА ДІЇ ДОБРИВ ТА БІОПРЕПАРАТУ |
| title | НАПРАВЛЕНІСТЬ БІОЛОГІЧНИХ ПРОЦЕСІВ У КОРЕНЕВІЙ ЗОНІ РОСЛИН КАРТОПЛІ ЗА ДІЇ ДОБРИВ ТА БІОПРЕПАРАТУ |
| title_alt | ORIENTATION OF BIOLOGICAL PROCESSES IN THE ROOT ZONE OF POTATO PLANTS UNDER THE INFLUENCE OF FERTILIZERS AND BIOLOGICAL PREPARATION |
| title_full | НАПРАВЛЕНІСТЬ БІОЛОГІЧНИХ ПРОЦЕСІВ У КОРЕНЕВІЙ ЗОНІ РОСЛИН КАРТОПЛІ ЗА ДІЇ ДОБРИВ ТА БІОПРЕПАРАТУ |
| title_fullStr | НАПРАВЛЕНІСТЬ БІОЛОГІЧНИХ ПРОЦЕСІВ У КОРЕНЕВІЙ ЗОНІ РОСЛИН КАРТОПЛІ ЗА ДІЇ ДОБРИВ ТА БІОПРЕПАРАТУ |
| title_full_unstemmed | НАПРАВЛЕНІСТЬ БІОЛОГІЧНИХ ПРОЦЕСІВ У КОРЕНЕВІЙ ЗОНІ РОСЛИН КАРТОПЛІ ЗА ДІЇ ДОБРИВ ТА БІОПРЕПАРАТУ |
| title_short | НАПРАВЛЕНІСТЬ БІОЛОГІЧНИХ ПРОЦЕСІВ У КОРЕНЕВІЙ ЗОНІ РОСЛИН КАРТОПЛІ ЗА ДІЇ ДОБРИВ ТА БІОПРЕПАРАТУ |
| title_sort | направленість біологічних процесів у кореневій зоні рослин картоплі за дії добрив та біопрепарату |
| topic | мікробіологічні процеси добрива азотфіксація емісія N2O і CO2 картопля |
| topic_facet | microbiological processes fertilizers nitrogen fixation N2O emission CO2 emission potatoes мікробіологічні процеси добрива азотфіксація емісія N2O і CO2 картопля |
| url | https://smic.in.ua/index.php/journal/article/view/164 |
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