ВИЯВЛЕННЯ, ПОШИРЕННЯ ТА ШКОДОЧИНІСТЬ МІКОПЛАЗМОЗУ ГРЕЧКИ
The paper presents the results of studies of spread and impact of Phytoplasma on the growth of buckwheat plants. Electron microscopic studies of Phytoplasma were conducted. The images of Phytoplasma established the possibility of a mixed infection, allowing further conduct targeted research and deve...
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| Date: | 2014 |
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| Main Authors: | , , , , |
| Format: | Article |
| Language: | English Ukrainian |
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Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine
2014
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| Online Access: | https://smic.in.ua/index.php/journal/article/view/175 |
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| Journal Title: | Agriciltural microbiology |
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Agriciltural microbiology| _version_ | 1871465668220551168 |
|---|---|
| author | Шевчук, В. К. Демченко, О. А. Юзвенко, Л. В. Радченко, В. Г. Бойко, А. Л. |
| author_facet | Шевчук, В. К. Демченко, О. А. Юзвенко, Л. В. Радченко, В. Г. Бойко, А. Л. |
| author_institution_txt_mv | [
{
"author": "В. К. Шевчук",
"institution": "Подільський державний аграрно-технічний університет"
},
{
"author": "О. А. Демченко",
"institution": "Інститут мікробіології і вірусології ім. Д. К. Заболотного НАН України"
},
{
"author": "Л. В. Юзвенко",
"institution": "Інститут мікробіології і вірусології ім. Д. К. Заболотного НАН України"
},
{
"author": "В. Г. Радченко",
"institution": "Інститут еволюційної екології НАН України"
},
{
"author": "А. Л. Бойко",
"institution": "Київський національний університет імені Тараса Шевченка"
}
] |
| author_sort | Шевчук, В. К. |
| baseUrl_str | https://smic.in.ua/index.php/journal/oai |
| collection | OJS |
| datestamp_date | 2026-07-22T10:10:43Z |
| description | The paper presents the results of studies of spread and impact of Phytoplasma on the growth of buckwheat plants. Electron microscopic studies of Phytoplasma were conducted. The images of Phytoplasma established the possibility of a mixed infection, allowing further conduct targeted research and development of methods to combat this pathogen. |
| doi_str_mv | 10.35868/1997-3004.19.78-82 |
| first_indexed | 2025-07-17T12:24:17Z |
| format | Article |
| fulltext |
IDENTIFICATION, SPREAD AND HARMFULNESS OF BUCKWHEAT
MYCOPLASMOSIS
V. K. Shevchuk1, O. A. Demchenko2,4, L. V. Yuzvenko2,
V. H. Radchenko4, A. L. Boiko3
1Podillia State Agrarian and Technical University
13, Shevchenka Str.; Kamianets-Podilskyi City, Khmelnytsk region, 32300, Ukraine
2Danylo Zabolotny Institute of Microbiology and Virology, NAS of Ukraine, Kyiv
154, Akademika Zabolotnoho Str., Kyiv City, MSP D03143, Ukraine
3Taras Shevchenko National University of Kyiv
60, Volodymyrska Str.; Kyiv City, 01601, Ukraine
4Institute for Evolutionary Ecology of NAS of Ukraine
37, Akademika Lebedieva Str.; Kyiv City, 03143, Ukraine
e-mail: spivak-spivak-n@ya.ru
Plant mycoplasmal diseases are
widespread in the world. A number of
Phytoplasmas are known which belong to
more than 30 groups and are pathogens for
diseases that affect more than 200 plant
species. It had been thought for a long time
that viruses cause them, although no
pathogens were isolated or visualized. In 1967
Japanese scientists [1] revealed the structures
morphologically similar to animal pathogens
– mycoplasma – in ultrathin sections of aster
plant phloem, diseased with chlorosis. Those
new plant pathogens were named
mycoplasma-like organisms and classified as
Mollicutes, i.e. bacteria without cell wall.
In the early 90-ies, based on
phylogenetic analysis of DNA sequences of
those organisms it was shown that they are a
great monophyletic (i.e. derived from one
ancestor) group within Mollicutes class and
the name “phytoplasma” was suggested for
them. Other representatives of this class were
Mycoplasmas, Acholeplasmas and
Spiroplasmas, closest to such bacteria as
Bacillus, Clostridium, Streptococcus. Current
trivial name “phytoplasma” was officially
adopted in 1994 at the 10th Congress of the
International Organization of
Mycoplasmologists to replace the term
“mycoplasma-like organisms” [2]. Today
phytoplasmas are separated as “Candidates
Phytoplasma” genus. Candidates category is
used to refer to organisms that cannot be
grown in vitro. The first 20 Candidates
species were attributed to this genus on the
basis that the identity of the DNA nucleotide
sequence of 16S rRNA gene was at least
97.5% [3].
These pathogens are well identified in
transmissible and scanning electron
microscopy. Thus, wheat and hops
phytoplasma were examined during the study
of homogenates and ultrathin sections of cells.
[4]
Phytoplasma cause diseases of
economically important crops, including
buckwheat – valuable cereal crop. Its fruits
contain 12-18% of protein, 2-4% of fat, 70-
85% of starch. For its nutritional value, taste
and dietary properties buckwheat cereal is
considered one of the most important food
products used in cooking to prepare different
dishes [5]. In addition, buckwheat is a rich
melliferous plant.
One of the factors limiting obtaining
high buckwheat yields is its affection by
diseases. Buckwheat phytoplasma infestation
belongs understudied diseases and was first
identified by S. F. Sydorova in 1965. At that
this pathogen was not visualized [6].
The objective of our research is to
study the features of this disease pathogen
spreading and to study its morphological
characteristics.
Materials and methods. To identify
buckwheat phytoplasma route survey of
buckwheat crops and stationary experiments
with buckwheat were conducted. Buckwheat
mycoplasmosis harmfulness was determined
by comparing the elements of
morphobiometric performance of diseased
and healthy plants.
To prove the pathogenicity of
buckwheat phytoplasma pathogen visual
diagnosis and engrafting of diseased plants
upon plant indicator (rosy periwinkle
Catharanthus roseus G. Don) were used. The
infection was performed by cleft grafting. The
observation was conducted during the month.
In order to visualize and localize the
pathogen electron microscopic examination
was carried out. The contrasting was
performed using uranyl acetate and
phosphotungstic acid (PTA).
To study mixed infection in
buckwheat plants (mycoplasma, fungi,
viruses, bacteria) conventional methods for
pathogen detection were used [7].
So, through the analysis of diseased
buckwheat plants we found and identified
phytoplasma (mycoplasma), allowing further
conducting targeted research and development
of pathogen control measures.
|
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| language | English Ukrainian |
| last_indexed | 2026-07-23T01:05:41Z |
| publishDate | 2014 |
| publisher | Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine |
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| spelling | oai:ojs2.smic.in.ua:article-1752026-07-22T10:10:43Z IDENTIFICATION, SPREAD AND HARMFULNESS OF BUCKWHEAT MYCOPLASMOSIS ВИЯВЛЕННЯ, ПОШИРЕННЯ ТА ШКОДОЧИНІСТЬ МІКОПЛАЗМОЗУ ГРЕЧКИ Шевчук, В. К. Демченко, О. А. Юзвенко, Л. В. Радченко, В. Г. Бойко, А. Л. buckwheat plant, Phytoplasma, mixed infection рослини гречки, фітоплазма, змішана інфекція The paper presents the results of studies of spread and impact of Phytoplasma on the growth of buckwheat plants. Electron microscopic studies of Phytoplasma were conducted. The images of Phytoplasma established the possibility of a mixed infection, allowing further conduct targeted research and development of methods to combat this pathogen. У статті вперше представлено результати досліджень щодо поширення і впливу фітоплазм на ріст і розвиток рослин гречки. Проведені електронно мікроскопічне дослідження фітоплазм, встановлена можливість змішаної інфекції, що дозволяє в подальшому проводити цілеспрямовані пошуки та розробку заходів боротьби з цим патогеном. Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine 2014-06-26 Article Article Рецензована Стаття application/pdf application/pdf https://smic.in.ua/index.php/journal/article/view/175 10.35868/1997-3004.19.78-82 Agricultural microbiology; Vol. 19 (2014): Agriciltural microbiology; 78-82 Сільськогосподарська мікробіологія; Том 19 (2014): Сільськогосподарська мікробіологія; 78-82 1997-3004 10.35868/1997-3004.19 en uk https://smic.in.ua/index.php/journal/article/view/175/233 https://smic.in.ua/index.php/journal/article/view/175/234 Авторське право (c) 2014 V. K. Shevchuk, O. A. Demchenko, L. V. Yuzvenko, V. H. Radchenko, A. L. Boiko https://creativecommons.org/licenses/by/4.0 |
| spellingShingle | рослини гречки фітоплазма змішана інфекція Шевчук, В. К. Демченко, О. А. Юзвенко, Л. В. Радченко, В. Г. Бойко, А. Л. ВИЯВЛЕННЯ, ПОШИРЕННЯ ТА ШКОДОЧИНІСТЬ МІКОПЛАЗМОЗУ ГРЕЧКИ |
| title | ВИЯВЛЕННЯ, ПОШИРЕННЯ ТА ШКОДОЧИНІСТЬ МІКОПЛАЗМОЗУ ГРЕЧКИ |
| title_alt | IDENTIFICATION, SPREAD AND HARMFULNESS OF BUCKWHEAT MYCOPLASMOSIS |
| title_full | ВИЯВЛЕННЯ, ПОШИРЕННЯ ТА ШКОДОЧИНІСТЬ МІКОПЛАЗМОЗУ ГРЕЧКИ |
| title_fullStr | ВИЯВЛЕННЯ, ПОШИРЕННЯ ТА ШКОДОЧИНІСТЬ МІКОПЛАЗМОЗУ ГРЕЧКИ |
| title_full_unstemmed | ВИЯВЛЕННЯ, ПОШИРЕННЯ ТА ШКОДОЧИНІСТЬ МІКОПЛАЗМОЗУ ГРЕЧКИ |
| title_short | ВИЯВЛЕННЯ, ПОШИРЕННЯ ТА ШКОДОЧИНІСТЬ МІКОПЛАЗМОЗУ ГРЕЧКИ |
| title_sort | виявлення, поширення та шкодочиність мікоплазмозу гречки |
| topic | рослини гречки фітоплазма змішана інфекція |
| topic_facet | buckwheat plant Phytoplasma mixed infection рослини гречки фітоплазма змішана інфекція |
| url | https://smic.in.ua/index.php/journal/article/view/175 |
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