ФІЗИКО-ХІМІЧНІ ВЛАСТИВОСТІ ІЗОЛЯТІВ ВІРУСУ СКРУЧУВАННЯ ЛИСТЯ КАРТОПЛІ В КУЛЬТУРАХ КЛІТИН ССАВЦІВ
The paper presents the research data of physical and chemical properties of potato leaf rolling virus isolates in the PEKV and BHK-21 cell cultures. It was shown that in mammalian cell cultures the isolates of potatoes leaf rolling virus were resistant to the action of lipid dissolving agents, cultu...
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| Date: | 2014 |
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| Main Authors: | , , |
| Format: | Article |
| Language: | English Ukrainian |
| Published: |
Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine
2014
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| Online Access: | https://smic.in.ua/index.php/journal/article/view/176 |
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| Journal Title: | Agriciltural microbiology |
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Agriciltural microbiology| _version_ | 1871465671270858752 |
|---|---|
| author | Бова, Т. О. Волкова, І. В. Решотько, Л. М. |
| author_facet | Бова, Т. О. Волкова, І. В. Решотько, Л. М. |
| author_institution_txt_mv | [
{
"author": "Т. О. Бова",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "І. В. Волкова",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "Л. М. Решотько",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
}
] |
| author_sort | Бова, Т. О. |
| baseUrl_str | https://smic.in.ua/index.php/journal/oai |
| collection | OJS |
| datestamp_date | 2026-07-22T10:10:43Z |
| description | The paper presents the research data of physical and chemical properties of potato leaf rolling virus isolates in the PEKV and BHK-21 cell cultures. It was shown that in mammalian cell cultures the isolates of potatoes leaf rolling virus were resistant to the action of lipid dissolving agents, cultural media with pH from 4.0 to 10.0 and were thermoresistant at 50 °C in the absence of divalent ions of magnesium. The virus inactivation temperature point along with the optimum temperature for its reproduction were established. |
| doi_str_mv | 10.35868/1997-3004.19.83-88 |
| first_indexed | 2025-07-17T12:24:17Z |
| format | Article |
| fulltext |
INVESTIGATION OF PHYSICAL AND CHEMICAL PROPERTIES OF
POTATO LEAFROLL VIRUS ISOLATES IN CULTURES OF MAMMALIAN
CELLS
T. O. Bova, I. V. Volkova, L. M. Reshotko
Potato leaf rolling virus (PLRV) is
among the most economically important
pathogenic viruses. Yield losses due to
infection by PLRV, depending on potato
variety, climate and soil conditions, range
from 20% to 90% [1; 2]. PLRV is common in
all countries, where potato is grown. Potato
leaf rolling virus affects a wide range of
plants (today, this list comprises about 40
species) belonging to different families:
Amaranthaceae, Cucurbitaceae,
Chenopodiaceae, Brassicaceae, Asteraceae,
Lamiaceae, Portulaceae, Nolanaceae,
Solanaceae, but the main virus host is potato
[3; 4]. Different types of crops: tobacco,
tomatoes, strawberry tomato and others are
also susceptible to virus. Potato leaf rolling
virus is found in weed and ornamental plants
which, due to their susceptibility may be
reserves of the virus in agrocenoses.
Great harm of PLRV is explained first
of all by rapid virus spread by insect vectors
and transmission vegetatively through tubers
that wholly or partly lose their germination
ability.
Three hazard zones were distinguished
in the area of PLRV distribution [5]. In the
zone of strong harmfulness the prevalence of
disease on potatoes is more than 40%, there
are frequent epiphytoties. In the area of
average harmfulness epiphytoties occur at the
intervals of 8-10 years and in the zone of low
harmfulness the disease does not exceed 10%.
The zone of strong harmfulness brings
together the countries of Central Asia, the
Caucasus, Kazakhstan, the North Caucasus,
the western regions of Ukraine. Average
harmfulness is characteristic for Belarus,
Moldova, Central Black Earth area,
Primorskii Krai, the Baltic countries,
Sakhalin, Altai, Nizhnii Novgorod, Ivanovo
and Novgorod regions. On the rest of potato
cultivation territory disease development does
not exceed 10%.
But the issue of harmfulness and
prevalence of the virus in different climatic
zones of Ukraine remains uncertain. In 1989-
1992 and 2008-2009 the prevalence of potato
viral diseases was studied on the basis of
Ustymivsk Experimental Station [6]. It was
shown that severe viral diseases, described
earlier as gothic, are complex diseases caused
by the compatible action of PLRV with
mosaic virus group. Thus, virus leaf rolling is
the most common and harmful disease in the
conditions of southern Woodlands of Ukraine.
Incidence and prevalence of potato
varieties and hybrids by PLRV is studied in
the countries of Europe, the Americas, Africa,
Asia, Australia and New Zealand. There is an
opinion about the cyclicity of epiphytoties
caused by PLRV and their relationship to
solar activity and droughts [7; 8].
PLRV has such features as extremely
low concentration in plant extracts,
determined by virus localization in the
phloem, inability of mechanical transmission
and small size of virions, which complicates
the study of virus isolates and diagnosis of
diseases that are caused by it.
To study the properties of the isolates
and strains of PLRV potato plants or plants-
indicators are used, to which the virus is
transferred from infected plants by grafting or
aphids. Aphids are previously fed on infected
plants or through membranes or virus
preparation is injected through micro-
injections [9]. Sometimes, for PLRV
reproduction, protoplasts of tobacco or potato
mesophile and poly-L-ornithine are involved,
which forms complex with viral particles and
facilitates protoplasts infection [10].
In 2009 we described the phenomenon
of PLRV productive infection in mammalian
cells cultures [11; 12]. According to the
results of further research neutralization and
identification reaction in cell culture was
suggested to indicate PLRV in plant material
[13].
In this regard, the objective of our
work was to separate potato leaf rolling virus
isolates into a pure culture from vegetating
plants, tubers and aphids and explore their
physicochemical properties.
Materials and methods. To separate
potato leaf rolling virus isolates the primary
seed growing plot of CJSC
“Chernihivelitkartoplia” was examined. 18
samples of potato leaves with potato leaf
rolling virus symptoms and aphids were
selected.
The separation of PLRV isolates was
conducted in cell cultures of finite line of pig
embryonic kidney (PEK) and finite line of
newborn hamster kidney (BHK-21), which
was grown in test tubes. Before the virus
introduction culture medium was removed
from test tubes, the monolayer was washed
twice with 0.15M NaCl or Hanks' solution.
Investigated samples of 0.1cm3, broken by
chloroform, were added and kept in an
incubator at 37°C for a contact within 1 hour.
Then the monolayer was washed with Hanks'
solution and supportive medium was poured.
Inoculated tubes with cells culture were
incubated in an incubator at 37°C until the
signs of virus cytopathic effect (CPE)
appeared. 3-5 blind passages were conducted
in cell cultures [11].
Virus thermoresistance was studied by
heating the samples on a water bath over 1
hour at 50°C in the presence of 1M MgCl2
and without it. Cold virus samples and heated
in the presence of divalent ions of magnesium
and without them were titrated [14].
The stability of viruses at different pH
of the medium was studied in PEKV cell
culture. For that, viruses were kept in the
medium with pH 2.0; 3.0; 4.0; 7.2; 10.0; 11.0
at room temperature for 10 minutes. Then pH
value was adjusted to 7.2 in all samples. Virus
sensitivity to acidic and alkaline pH was
determined by the difference of virus titres
compared with control [14].
Temperature effect on the
performance of PLRV – animal cells system
was studied in BHK-21 cell culture. Cell
culture monolayer was inoculated with
Sednivskyi strain of potato leaf rolling virus
at 1 TCD50. The incubation was carried out at
2, 10, 24 and 37 °C. Degenerative changes of
the monolayer, time of their occurrence and
infectious virus activity were recorded [11].
So, four isolates of potato leaf rolling
virus were identified in mammalian cell
cultures that occurred to be resistant to lipid
dissolving agents, cultural media with pH
from 4.0 to 10.0 and were thermoresistant at
50°C in the absence of divalent ions of
magnesium, TIP is within the range of 60-
65°C. Optimum temperature for PLRV
reproduction is +24°C and above.
|
| id | oai:ojs2.smic.in.ua:article-176 |
| institution | Agriciltural microbiology |
| keywords_txt_mv | keywords |
| language | English Ukrainian |
| last_indexed | 2026-07-23T01:05:44Z |
| publishDate | 2014 |
| publisher | Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine |
| record_format | ojs |
| resource_txt_mv | smicinua/b8/6859b7e52fded4c77fa5e71552d77eb8.pdf |
| spelling | oai:ojs2.smic.in.ua:article-1762026-07-22T10:10:43Z INVESTIGATION OF PHYSICAL AND CHEMICAL PROPERTIES OF POTATO LEAFROLL VIRUS ISOLATES IN CULTURES OF MAMMALIAN CELLS ФІЗИКО-ХІМІЧНІ ВЛАСТИВОСТІ ІЗОЛЯТІВ ВІРУСУ СКРУЧУВАННЯ ЛИСТЯ КАРТОПЛІ В КУЛЬТУРАХ КЛІТИН ССАВЦІВ Бова, Т. О. Волкова, І. В. Решотько, Л. М. potato, potato leafroll virus, mammalian cells culture, physico-chemical properties картопля, вірус скручування листя картоплі, культура клітин ссавців, фізико-хімічні властивості The paper presents the research data of physical and chemical properties of potato leaf rolling virus isolates in the PEKV and BHK-21 cell cultures. It was shown that in mammalian cell cultures the isolates of potatoes leaf rolling virus were resistant to the action of lipid dissolving agents, cultural media with pH from 4.0 to 10.0 and were thermoresistant at 50 °C in the absence of divalent ions of magnesium. The virus inactivation temperature point along with the optimum temperature for its reproduction were established. Наведено результати дослідження фізико-хімічних властивостей ізолятів вірусу скручування листя картоплі в культурах клітин СНЕВ та ВНК-21. Показано, що в культурах клітин ссавців ізоляти вірусу виявилися стійкими до ліпідорозчинників, до середовищ із значеннями рН від 4,0 до 10,0 та терморезистентними при 50 °С за відсутності двовалентних іонів магнію. Визначено точку температурної інактивації вірусу та оптимальну температуру для репродукції. Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine 2014-06-26 Article Article Рецензована Стаття application/pdf application/pdf https://smic.in.ua/index.php/journal/article/view/176 10.35868/1997-3004.19.83-88 Agricultural microbiology; Vol. 19 (2014): Agriciltural microbiology; 83-88 Сільськогосподарська мікробіологія; Том 19 (2014): Сільськогосподарська мікробіологія; 83-88 1997-3004 10.35868/1997-3004.19 en uk https://smic.in.ua/index.php/journal/article/view/176/235 https://smic.in.ua/index.php/journal/article/view/176/236 Авторське право (c) 2014 T. O. Bova, I. V. Volkova, L. M. Reshotko https://creativecommons.org/licenses/by/4.0 |
| spellingShingle | картопля вірус скручування листя картоплі культура клітин ссавців фізико-хімічні властивості Бова, Т. О. Волкова, І. В. Решотько, Л. М. ФІЗИКО-ХІМІЧНІ ВЛАСТИВОСТІ ІЗОЛЯТІВ ВІРУСУ СКРУЧУВАННЯ ЛИСТЯ КАРТОПЛІ В КУЛЬТУРАХ КЛІТИН ССАВЦІВ |
| title | ФІЗИКО-ХІМІЧНІ ВЛАСТИВОСТІ ІЗОЛЯТІВ ВІРУСУ СКРУЧУВАННЯ ЛИСТЯ КАРТОПЛІ В КУЛЬТУРАХ КЛІТИН ССАВЦІВ |
| title_alt | INVESTIGATION OF PHYSICAL AND CHEMICAL PROPERTIES OF POTATO LEAFROLL VIRUS ISOLATES IN CULTURES OF MAMMALIAN CELLS |
| title_full | ФІЗИКО-ХІМІЧНІ ВЛАСТИВОСТІ ІЗОЛЯТІВ ВІРУСУ СКРУЧУВАННЯ ЛИСТЯ КАРТОПЛІ В КУЛЬТУРАХ КЛІТИН ССАВЦІВ |
| title_fullStr | ФІЗИКО-ХІМІЧНІ ВЛАСТИВОСТІ ІЗОЛЯТІВ ВІРУСУ СКРУЧУВАННЯ ЛИСТЯ КАРТОПЛІ В КУЛЬТУРАХ КЛІТИН ССАВЦІВ |
| title_full_unstemmed | ФІЗИКО-ХІМІЧНІ ВЛАСТИВОСТІ ІЗОЛЯТІВ ВІРУСУ СКРУЧУВАННЯ ЛИСТЯ КАРТОПЛІ В КУЛЬТУРАХ КЛІТИН ССАВЦІВ |
| title_short | ФІЗИКО-ХІМІЧНІ ВЛАСТИВОСТІ ІЗОЛЯТІВ ВІРУСУ СКРУЧУВАННЯ ЛИСТЯ КАРТОПЛІ В КУЛЬТУРАХ КЛІТИН ССАВЦІВ |
| title_sort | фізико-хімічні властивості ізолятів вірусу скручування листя картоплі в культурах клітин ссавців |
| topic | картопля вірус скручування листя картоплі культура клітин ссавців фізико-хімічні властивості |
| topic_facet | potato potato leafroll virus mammalian cells culture physico-chemical properties картопля вірус скручування листя картоплі культура клітин ссавців фізико-хімічні властивості |
| url | https://smic.in.ua/index.php/journal/article/view/176 |
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