ВПЛИВ ВНУТРІШНЬОГО СЕРЕДОВИЩА ОРГАНІЗМУ ТВАРИН НА ВЛАСТИВОСТІ БАКТЕРІЙ-ПРОБІОТИКІВ
The paper depicts the results of the study of influence of internal environment of animals on preservation of initial biological properties of probiotic bacteria. The increased by 10–15 % antagonistic activity was observed in Bacillus subtilis 44-p strain after its translocation to the laboratory ra...
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| Datum: | 2014 |
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| Sprache: | Englisch Ukrainisch |
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Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine
2014
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| Online Zugang: | https://smic.in.ua/index.php/journal/article/view/177 |
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| Назва журналу: | Agriciltural microbiology |
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Agriciltural microbiology| _version_ | 1871465673776955392 |
|---|---|
| author | Агеєв, В. О. |
| author_facet | Агеєв, В. О. |
| author_institution_txt_mv | [
{
"author": "В. О. Агеєв",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
}
] |
| author_sort | Агеєв, В. О. |
| baseUrl_str | https://smic.in.ua/index.php/journal/oai |
| collection | OJS |
| datestamp_date | 2026-07-22T10:10:43Z |
| description | The paper depicts the results of the study of influence of internal environment of animals on preservation of initial biological properties of probiotic bacteria. The increased by 10–15 % antagonistic activity was observed in Bacillus subtilis 44-p strain after its translocation to the laboratory rabbits while for B. subtilis B3 the changes in the morphology of colonies (size and shape) were noticed. Nevertheless, the properties of the studied lactic acid bacteria after exposition to the internal environment of the macroorganism remain unchanged. |
| doi_str_mv | 10.35868/1997-3004.19.89-94 |
| first_indexed | 2025-07-17T12:24:18Z |
| format | Article |
| fulltext |
THE EFFECT OF INTERNAL ENVIRONMENT OF ANIMALS ON
PROPERTIES OF PROBIOTIC BACTERIA
V. O. Aheyev
From the very beginning of
microbiological science an idea of the depth
of microorganism interaction with its
microflora was limited for a long time to the
confidence that normocenosis representatives
in all their diversity live exclusively on the
surfaces of a macroorganism, mainly on the
epithelium of the gastrointestinal tract (GIT).
The presence of any microorganisms in blood,
and even more so – in the tissues of
parenchymal organs was a priori interpreted
solely as the sign of progressive pathologic
state and (or) as a result of a substantial
weakening of organism protective capacity
[1].
Only in the late twentieth century,
mostly in foreign scientific literature, there
appear more experimental data that
contradicted with the traditional idea of the
mechanisms of probiotics action on animals
and humans. The authors from different
countries noted instances of probiotic
preparations efficacy that were are not subject
to explanation in terms of conventional vision
– not only to treat infections of the digestive
tract at the intestine colonization, but also in
the treatment of pathological processes
located outside the GIT, namely: external
cover injuries, postoperative suppurations,
pyelonephritis, respiratory diseases, etc. [2;
3].
The discovered phenomenon of
bacterial penetration into blood and
parenchymal organs through the uninjured
wall of the gastrointestinal tract was called
“bacterial translocation” and was first
described by American researcher R D. Berg
in 1985 [4].
At the beginning of the second
millennium, V. I. Nikitenko et al. [5], with
the help of two strains of hay bacillus
radiolabeled with H3-leucine, proved the
possibility of probiotic bacteria translocation
from the gastrointestinal tract into blood and
lymph flow. According to V. I. Nikitenko [6]
V. V. Smirnov et al. [7], V. A. Kopylov,
V. V. Zakharov [8], L. de Souza et al. [9] the
translocation may be a natural defense
mechanism conditional to microorganism
functions and not bacteria properties. Thus,
there is evidence, that probiotic strains
bacterial translocation and short-term
asymptomatic bacteraemia, caused by it, can
be one of important factors for activation and
maintenance of nonspecific resistance of
microorganism itself on a sufficient level.
Today the development of new
approaches to the strategy and tactics of
creation and use of biological preparations of
bacterial origin, including probiotics, is
urgent. And due to the comprehensive
research of mutual influence of
macroorganism and probiotic bacteria it
becomes possible to create probiotic
veterinary and medical preparations intended
for the prevention and treatment of not only
digestive system disorders, but many diseases
of farm animals and humans, localized
outside gastrointestinal tract [10-12].
Materials and methods. Probiotic
strains of Bacillus subtilis 44-p (the basis of
BPS-44 preparation), B. subtilis B3,
Lactobacillus plantarum L5 (part of BPS-L
preparation), L. plantarum Lc-18
(perspective) were used in the study.
Pathogenic test cultures Staphylococcus
aureus 23, Escherichia coli 14/1, Salmonella
typhimurium 12, Shigella sonnei 22 from the
collection of the Probiotics Laboratory of the
Institute of Agricultural Microbiology and
Agroindustrial Manufacture of NAAS were
used to determine the antagonistic activity.
The cultivation probiotic strains of
aerobic bacilli and pathogenic test-cultures
(for the determination of antagonistic activity)
was performed on standard nutrient media –
meat-peptone broth (MPB) and meat-peptone
agar (MPA); lactic acid bacteria - on De Man
medium (MRS) [13]. Daily culture was used
for studies of bacterial translocation.
The investigation of the influence of
translocation process on cultural-
morphological and physiological and
biochemical properties of bacteria-probiotics
was conducted on laboratory rabbits. Groups
of animals were formed for that – one control
and four experimental (by the number of
investigated strains), 5 animals in each group.
Before the experiment, blood from all animals
was taken and bacteriologically examined for
the presence of aerobic bacilli and lactic acid
bacteria in it.
The investigated strains of bacteria
were administered to animals per os, once,
with water at the dose of 3×108CFU, drinking
water was administered to control animals.
The separation of bacteria from the
internal environment of experimental animals
was conducted in the moments of their
greatest accumulation in blood that had been
found during previous studies. For aerobic
bacilli that period is 5-10 min. from the
moment of their oral administration; for lactic
acid bacteria – 12-24 hours. Pure cultures
were isolated by limiting dilution using
appropriate culture media, inoculation was
performed from the dilutions of 100 (native
material) to 10-8 (to obtain isolated colonies).
Housing, feeding, care and all
manipulations with laboratory animals were
carried out under the European Convention
“For the Protection of Vertebrate Animals
used for Experimental and other Scientific
Purposes” (Strasbourg, 1986) [19] and
“General Ethical Principles of Experiments on
Animals” approved at the first National
Congress on Bioethics (Kyiv, 2001) [20].
Experiments were conducted in compliance
with the principles of humanity set out in the
European Community Directive [21].
Cultural-morphological (morphology
of cells and colonies, peculiarities of the
growth on nutrient media) and physiological
and biochemical (exoenzymes activity, the
ability to ferment various sources of Carbon,
the amount of formed acid, antagonistic
activity) properties were studied in the initial
probiotic strains and cultures isolated from
blood and organs of experimental animals
[14-18].
Mathematical processing of all
obtained results that are quantified, were done
by the methods of variation statistics using
Student t-test and correlation coefficients
[22].
So, the translocation process of
researched probiotic lactic acid bacteria into
the internal environment of warm-blooded
animals does not cause such changes in
cultural-morphological characteristics,
physiological and biochemical properties of
bacteria that could be detected with the help
of used methods; it can be explained by
greater stability of their phenotype determined
during phylogenesis, and higher heat
resistance.
|
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| institution | Agriciltural microbiology |
| keywords_txt_mv | keywords |
| language | English Ukrainian |
| last_indexed | 2026-07-23T01:05:47Z |
| publishDate | 2014 |
| publisher | Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine |
| record_format | ojs |
| resource_txt_mv | smicinua/5c/81dde41efbd1036d0c2fd556ac70d35c.pdf |
| spelling | oai:ojs2.smic.in.ua:article-1772026-07-22T10:10:43Z THE EFFECT OF INTERNAL ENVIRONMENT OF ANIMALS ON PROPERTIES OF PROBIOTIC BACTERIA ВПЛИВ ВНУТРІШНЬОГО СЕРЕДОВИЩА ОРГАНІЗМУ ТВАРИН НА ВЛАСТИВОСТІ БАКТЕРІЙ-ПРОБІОТИКІВ Агеєв, В. О. Bacillus subtilis, lactic acid bacteria, bacterial translocation, blood, cultural-morphological characteristics, physiological and biochemical properties аеробні бацили, молочнокислі бактерії, бактеріальна транслокація, кров, культурально-морфологічні ознаки, фізіолого-біохімічні властивості The paper depicts the results of the study of influence of internal environment of animals on preservation of initial biological properties of probiotic bacteria. The increased by 10–15 % antagonistic activity was observed in Bacillus subtilis 44-p strain after its translocation to the laboratory rabbits while for B. subtilis B3 the changes in the morphology of colonies (size and shape) were noticed. Nevertheless, the properties of the studied lactic acid bacteria after exposition to the internal environment of the macroorganism remain unchanged. Представлено результати вивчення впливу внутрішнього середовища організму тварин на збереженість вихідних біологічних властивостей пробіотичних бактерій. Встановлено, що у штаму Bacillus subtilis 44-p після транслокації в організм лабораторних кролів на 10–15 % збільшується антагоністична активність, у B. subtilis В3 спостерігаються зміни морфології колоній (розміру та форми). Разом з тим, властивості досліджених молочнокислих бактерій після перебування у внутрішньому середовищі макроорганізму залишаються без змін. Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine 2014-06-26 Article Article Рецензована Стаття application/pdf application/pdf https://smic.in.ua/index.php/journal/article/view/177 10.35868/1997-3004.19.89-94 Agricultural microbiology; Vol. 19 (2014): Agriciltural microbiology; 89-94 Сільськогосподарська мікробіологія; Том 19 (2014): Сільськогосподарська мікробіологія; 89-94 1997-3004 10.35868/1997-3004.19 en uk https://smic.in.ua/index.php/journal/article/view/177/237 https://smic.in.ua/index.php/journal/article/view/177/238 Авторське право (c) 2014 V. O. Aheyev https://creativecommons.org/licenses/by/4.0 |
| spellingShingle | аеробні бацили молочнокислі бактерії бактеріальна транслокація кров культурально-морфологічні ознаки фізіолого-біохімічні властивості Агеєв, В. О. ВПЛИВ ВНУТРІШНЬОГО СЕРЕДОВИЩА ОРГАНІЗМУ ТВАРИН НА ВЛАСТИВОСТІ БАКТЕРІЙ-ПРОБІОТИКІВ |
| title | ВПЛИВ ВНУТРІШНЬОГО СЕРЕДОВИЩА ОРГАНІЗМУ ТВАРИН НА ВЛАСТИВОСТІ БАКТЕРІЙ-ПРОБІОТИКІВ |
| title_alt | THE EFFECT OF INTERNAL ENVIRONMENT OF ANIMALS ON PROPERTIES OF PROBIOTIC BACTERIA |
| title_full | ВПЛИВ ВНУТРІШНЬОГО СЕРЕДОВИЩА ОРГАНІЗМУ ТВАРИН НА ВЛАСТИВОСТІ БАКТЕРІЙ-ПРОБІОТИКІВ |
| title_fullStr | ВПЛИВ ВНУТРІШНЬОГО СЕРЕДОВИЩА ОРГАНІЗМУ ТВАРИН НА ВЛАСТИВОСТІ БАКТЕРІЙ-ПРОБІОТИКІВ |
| title_full_unstemmed | ВПЛИВ ВНУТРІШНЬОГО СЕРЕДОВИЩА ОРГАНІЗМУ ТВАРИН НА ВЛАСТИВОСТІ БАКТЕРІЙ-ПРОБІОТИКІВ |
| title_short | ВПЛИВ ВНУТРІШНЬОГО СЕРЕДОВИЩА ОРГАНІЗМУ ТВАРИН НА ВЛАСТИВОСТІ БАКТЕРІЙ-ПРОБІОТИКІВ |
| title_sort | вплив внутрішнього середовища організму тварин на властивості бактерій-пробіотиків |
| topic | аеробні бацили молочнокислі бактерії бактеріальна транслокація кров культурально-морфологічні ознаки фізіолого-біохімічні властивості |
| topic_facet | Bacillus subtilis lactic acid bacteria bacterial translocation blood cultural-morphological characteristics physiological and biochemical properties аеробні бацили молочнокислі бактерії бактеріальна транслокація кров культурально-морфологічні ознаки фізіолого-біохімічні властивості |
| url | https://smic.in.ua/index.php/journal/article/view/177 |
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