ВПЛИВ ДЖЕРЕЛ БІОГЕННИХ ЕЛЕМЕНТІВ НА РІСТ BRADYRHIZOBIUM JAPONICUM І AZOSPIRILLUM BRASILENSE ЗА ЇХ СУМІСНОГО КУЛЬТИВУВАННЯ
The paper presents the study of nutrient sources influence on the growth of Bradyrhizobium japonicum and Azospirillum brasilense at their co-cultivation. It was shown that at cultivation of diazotrophs in nutrient media with different concentrations of its components, soybean rhizobia had uneven gro...
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Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine
2014
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| author | Козар, С. Ф. |
| author_facet | Козар, С. Ф. |
| author_institution_txt_mv | [
{
"author": "С. Ф. Козар",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
}
] |
| author_sort | Козар, С. Ф. |
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| collection | OJS |
| datestamp_date | 2026-07-22T10:10:44Z |
| description | The paper presents the study of nutrient sources influence on the growth of Bradyrhizobium japonicum and Azospirillum brasilense at their co-cultivation. It was shown that at cultivation of diazotrophs in nutrient media with different concentrations of its components, soybean rhizobia had uneven growth activity. Glucose and molasses was observed to have the greatest impact on the rhizobia count. The results obtained are used as the basis for the optimization of culture medium for co-cultivation of rhizobia and azospirills. |
| doi_str_mv | 10.35868/1997-3004.18.75-86 |
| first_indexed | 2025-07-17T12:24:20Z |
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| fulltext |
1
THE EFFECT OF NUTRIENT SOURCES ON GROWTH OF BRADYRHIZOBIUM
JAPONICUM AND AZOSPIRILLUM BRASILENSE AT CO-CULTIVATION
Kozar S. F.
Institute of Agricultural Microbiology and AgroIndustrial Production NAAS
97, Shevchenko str., Chernihiv, 14027
The paper presents the study of nutrient sources influence on the growth of
Bradyrhizobium japonicum and Azospirillum brasilense at their co-cultivation. It was shown
that at cultivation of diazotrophs in nutrient media with different concentrations of its
components, soybean rhizobia had uneven growth activity. Glucose and molasses was
observed to have the greatest impact on the rhizobia count. The results obtained are used as
the basis for the optimization of culture medium for co-cultivation of rhizobia and azospirills.
Key words: Bradyrhizobium japonicum, Azospirillum brasilense, growth, nutrients,
cultural medium, cultivation.
Beneficial soil microorganisms including rhizobia bacteria used as the agents for
different microbial preparations used in modern crop growing technologies play an important
role in plant nutrition [1]. The effectiveness of biological preparations highly depends on the
titer and functional activity component microorganisms [2]. Thus, it is very important to
develop the means that will improve growth activity of diazotrophs ensuring their high
nitrogenase activity.
Use of mixed microorganisms’ cultures might be one of the ways to enhance the
growth of nitrogen fixing bacteria and increase their functional activity. According to our
data, and the results obtained by other authors [3, 4] creation of biological preparation for
soybeans, which would contain nodule bacteria Bradyrhizobium japonicum and associative
diazotroph Azospirillum brasilense can be very promising. Thus, at co-inoculation of
soybeans with these microorganisms the increase of size and weight of individual nodules on
the roots of plants as well as nitogen fixing activity was observed as compared to the pure
rhizobia cultures. Co-cultivation of B. japonicum and A. brasilense also results in higher
growth activity of microorganisms, leading to the higher titer of inoculant. At the same time,
different nutrient requirements of rhizobia and azospiryls result in number of problems at
medium optimization for co-cultivation of diazotrophs.
Based on the foregoing, our research was aimed to study the effect of different nutrient
sources on the growth of Bradyrhizobium japonicum and Azospirillum brasilense in mixed
culture.
Materials and methods. Research objects: Bradyrhizobium japonicum M-8 [5],
Azospirillum brasilense 18-2 [6], Azospirillum brasilense 410 [7]. All microorganisms were
obtained from the National Collection of Useful Soil Microorganisms of Institute of
Agricultural Microbiology and AgroIndustrial Production NAAS.
2
The number of B. japonicum was determined using cup method by deep inoculation of
suspension in agar medium on pea broth [8]. The number of A. brasilense was determined
using cup method in agar medium, g/dm3, potato broth – 1, malic acid – 3.6, sucrose – 2.5;
pH – 7.2-7.4 (refined with NaOH solution), agar – 11 g.
Petri dishes were kept in an incubator at 28.0 ± 2.0 °C. B. japonicum colonies were
counted on the tenth day, A. brasilense – on the fourth day.
For co-cultivation the medium with dry yeast, glucose, molasses, (NH4)2SO4, KH2PO4,
K2HPO4 ∙ 3H2O, MgSO4 ∙ 7H2O, CaCO3 was used.
The following variables were studied: (NH4)2SO4 at concentrations of 0.05 g/dm3 to
3.0 g/dm3, KH2PO4 – 0.01 g/dm3 to 0.55 g/dm3, K2HPO4 ∙ 3H2O – 0.01 g/dm3 to 0.55 g/dm3,
molasses – 1.0 g/dm3 to 45.0 g/dm3, glucose – from 2.0 g/dm3 to 20.0 g/dm3 yeast feed - 1.0
g/dm3 to 10.0 g/dm3, MgSO4 ∙ 7H2O – 0.01 g/dm3 to 0.5 g/dm3.
Bacteria were cultivated in 500 cm3 vessels on a shaker with 240 rpm frequency and
temperature 28.0 ± 2.0 °C. B. japonicum and A. brasilense were used at 1:1 ratio and
cultivated for 72 hours.
Statistical analysis of the experimental data was performed using Statistica 6.0 software
package.
Results. Cultivation of rhizobia in nutrient media with different concentrations of its
components, had resulted in uneven growth activity of soybean nodule bacteria. Thus, while
determining optimal concentrations of nitrogen sources it was revealed that the highest
growth activity of B. japonicum M-8 was observed at cultivation of microorganisms in the
nutrient medium with a mass fraction of (NH4)2SO4 – 1.0 g/dm3 (Table 1). Reduction of the
mass fraction diammonium sulfate in the nutrient medium had led to the decrease of B.
japonicum number. C0-cultivation of rhizobia with A. brasilense 18-2 in the medium with
(NH4)2SO4 as the nitrogen source with a mass fraction of 0.5 g/dm3, 0.1 g/dm3, 0.05 g/dm3 the
number of B. japonicum M-8 was lower, respectively by 16%, 68% and 92% compared to the
highest obtained number of rhizobia in the experiment. The similar effect on the growth of
rhizobia was observed using smaller content of diammonium sulfate co-cultured with A.
brasilense 410: the number of B. japonicum had decreased by 14%, 20% and 51%
respectively compared to the highest obtained number of these bacteria.
Co-cultivation of B. japonicum M-8 with A. brasilense 18-2 in the nutrient medium
with high content of nitrogen had resulted in reduction of the rhizobia number by 8% (at 1.5
g/dm3 and 2.0 g/dm3) and 32% (at 3.0 g/dm3). Joint cultivation of B. japonicum M-8 with A.
brasilense 410, at higher content of diammonium sulfate the reduction trend of rhizobia
number reduction was observed.
While determining the effect of optimal concentration of phosphorus source for
microorganisms nutrition it was noticed that the highest growth activity of B. japonicum M-8
was observed at their co-cultivation in culture medium with a mass fraction of KH2PO4 – 0.15
g/dm3, 0.25 g/dm3 and 0.35 g/dm3 (Table 2).
3
Table 1. Effect of nitrogen source on the growth of B. japonicum and A. brasilense
in liquid nutrient media upon their co-cultivation
Concentrations
(NH4)2SO4,
g/dm3
Number of bacteria, billion cells / cm3
B. japonicum
М-8
A. brasilense
18-2
B. japonicum
М-8
A. brasilense
410
0,05 0,3 ± 0,12 2,7 ± 0,30 1,7 ± 0,15 2,7 ± 0,15
0,1 1,2 ± 0,20 3,5 ± 0,12 2,8 ± 0,18 3,0 ± 0,12
0,5 3,1 ± 0,20 3,6 ± 0,12 3,0 ± 0,15 3,3 ± 0,12
1,0 3,7 ± 0,15* 3,8 ± 0,15 3,5 ± 0,18 3,7 ± 0,15
1,5 3,4 ± 0,12 3,6 ± 0,15 3,4 ± 0,21 3,5 ± 0,15
2,0 3,4 ± 0,20 3,4 ± 0,15 3,3 ± 0,15 3,4 ± 0,15
3,0 2,5 ± 0,20 3,4 ± 0,15 3,2 ± 0,15 2,7 ± 0,15
*Note. The highest number of bacteria (within the limits of experimental error) is
highlightened in bold
The data obtained had showed that the highest number of rhizobia were observed at
KH2PO4 concentrations 0.15 g/dm3 and 0.25 g/dm3, but upon the increase of salt mass
fraction to 0.45 g/dm3 the titer of B. japonicum M-8 cultured together with A. brasilense 18-2,
was reduced by 14%.
The highest titer of B. japonicum M-8, co-cultivated with A. brasilense 410 was seen in
the medium containing KH2PO4 – 0.05 g/dm3, 0.15 g/dm3 and 0.25 g/dm3. Decrease of
KH2PO4 mass fraction to 0.01 g/dm3 had resulted in reduction of rhizobia number by 26%,
while increasing of its mass fraction to 0.35 g/dm3, 0.45 g/dm3 and 0.55 g/dm3 the studied
index had decreased, respectively, by 14%, 26% and 34%. Presented data had indicated that
co-cultivation of B. japonicum M-8 with azospirills, the recommended dose of KH2PO4 is
0.15 g/dm3.
Efficiency study of different K2HPO4 × 3H2O concentrations had showed that the
highest titer of B. japonicum M-8 was observed within the 0.15 g/dm3 and 0.25 g/dm3 of
dipotassium hydrogen. Both increase or decrease in the content of K2HPO4 × 3H2O in the
medium, had significantly reduced the titer of rhizobia.
Molasses and glucose were used for determination of the optimum mass fractions of
carbon sources in the culture medium. The highest growth activity of B. japonicum M-8, co-
cultured with A. brasilense 18-2 was observed in variants with 10.0 g/dm3, 15.0 g/dm3 and
20.0 g/dm3 of molasses (Table 3). At this, the rhizobia titer had ranged from 3.6 billion cells /
cm3 to 3.7 billion cells / cm3.
4
Table 2. Effect of phosphorus sources on the growth of B. japonicum and A. brasilense
in liquid nutrient medium at their co-cultivation
Phosphorous
concentrations in
phosphates form,
g/dm3
Number of bacteria, billion cells / cm3
B. japonicum
М-8
A. brasilense
18-2
B. japonicum
М-8
A. brasilense
410
KH2PO4
0,01 0,8 ± 0,12 2,2 ± 0,18 2,6 ± 0,23 2,6 ± 0,20
0,05 1,9 ± 0,18 3,5 ± 0,09 3,4 ± 0,09 3,3 ± 0,09
0,15 3,7 ± 0,03 3,7 ± 0,12 3,5 ± 0,25 3,6 ± 0,23
0,25 3,7 ± 0,15 3,8 ± 0,15 3,5 ± 0,18 3,7 ± 0,15
0,35 3,5 ± 0,15 3,7 ± 0,15 3,0 ± 0,09 3,5 ± 0,18
0,45 3,2 ± 0,12 3,6 ± 0,15 2,6 ± 0,15 3,0 ± 0,15
0,55 2,7 ± 0,12 3,3 ± 0,22 2,3 ± 0,12 3,0 ± 0,24
K2HPO4×3H2O
0,01 0,7 ± 0,15 1,7 ± 0,24 1,8 ± 0,12 2,4 ± 0,18
0,05 1,6 ± 0,18 3,5 ± 0,15 2,7 ± 0,35 3,1 ± 0,12
0,15 3,7 ± 0,07 3,6 ± 0,18 3,4 ± 0,26 3,5 ± 0,18
0,25 3,7 ± 0,15 3,8 ± 0,15 3,5 ± 0,18 3,7 ± 0,15
0,35 3,5 ± 0,15 3,7 ± 0,12 3,3 ± 0,15 3,6 ± 0,15
0,45 3,3 ± 0,15 3,7 ± 0,15 2,8 ± 0,12 3,3 ± 0,12
0,55 2,0 ± 0,09 3,5 ± 0,17 2,5 ± 0,15 2,7 ± 0,17
Molasses concentration range for B. japonicum M-8 co-cultured with A. brasilense 410
was broad – from 10.0 g/dm3 to 25.0 g/dm3, while rhizobia titer had ranged from 3.2 billion
cells / cm3 to 3.5 billion cells / cm3. Either decrease or increase the mass fraction of molasses
in the medium, the number of B. japonicum M-8 had significantly decreased. For all
investigated molasses concentrations, the 10.0 g/dm3 is recommended for rhizobia co-
cultivation with azospirills.
5
Table 3. Effect of carbon source on the growth of B. japonicum and A. brasilense
in liquid nutrient medium at their co-cultivation
Concentration of
carbon source,
g/dm3
Number of bacteria, billion cells / cm3
B. japonicum
М-8
A. brasilense
18-2
B. japonicum
М-8
A. brasilense
410
Molasses
1,0 2,6 ± 0,17 0,8 ± 0,15 2,6 ± 0,20 2,2 ± 0,20
5,0 3,0 ± 0,15 2,9 ± 0,12 3,1 ± 0,12 3,0 ± 0,09
10,0 3,7 ± 0,15 3,8 ± 0,15 3,5 ± 0,18 3,7 ± 0,15
15,0 3,6 ± 0,07 3,7 ± 0,18 3,4 ± 0,18 3,8 ± 0,15
20,0 3,6 ± 0,12 3,7 ± 0,12 3,4 ± 0,15 3,6 ± 0,17
25,0 3,2 ± 0,15 3,7 ± 0,23 3,2 ± 0,15 3,4 ± 0,09
30,0 3,3 ± 0,12 3,6 ± 0,15 2,7 ± 0,15 3,1 ± 0,12
35,0 2,6 ± 0,15 3,4 ± 0,09 2,2 ± 0,12 2,3 ± 0,12
40,0 2,3 ± 0,15 2,7 ± 0,12 2,3 ± 0,15 2,0 ± 0,19
45,0 1,2 ± 0,24 2,1 ± 0,21 1,8 ± 0,17 2,7 ± 0,15
Glucose
2,0 1,7 ± 0,15 3,4 ± 0,23 2,1 ± 0,09 3,4 ± 0,15
4,0 2,8 ± 0,24 3,4 ± 0,21 2,5 ± 0,15 3,4 ± 0,12
6,0 3,3 ± 0,18 3,5 ± 0,20 3,1 ± 0,12 3,5 ± 0,12
8,0 3,5 ± 0,12 3,4 ± 0,20 3,4 ± 0,12 3,5 ± 0,15
10,0 3,8 ± 0,09 3,5 ± 0,12 3,5 ± 0,12 3,6 ± 0,12
12,0 3,7 ± 0,15 3,5 ± 0,21 3,5 ± 0,18 3,7 ± 0,15
14,0 3,7 ± 0,09 3,3 ± 0,15 3,5 ± 0,20 3,5 ± 0,17
16,0 3,7 ± 0,22 3,5 ± 0,09 3,6 ± 0,15 3,3 ± 0,18
18,0 3,9 ± 0,09 3,3 ± 0,09 3,5 ± 0,15 3,1 ± 0,17
20,0 3,9 ± 0,12 3,2 ± 0,12 3,4 ± 0,15 3,1 ± 0,21
Rhizobia cultivation in medium with glucose concentrations ranging from 10.0 g/dm3
to 20.0 g/dm3, the number of B. japonicum M-8 was at the level of 3.7 billion cells / cm3 to
3.9 billion cells / cm3 at co-cultivation A. brasilense 18-2 and from 3.4 billion cells / cm3 to
3.6 billion cells / cm3 – with A. brasilense 410. Reduction of glucose content in medium, the
number of soybean rhizobia had also reduced. Glucose concentration at 10.0 g/dm3, is
recommended. The increasing of glucose content in the medium is economically
unreasonable as the changes of bacteria number was within the error.
6
As it was showed in our previous studies it was recommended the use of dry yeasts for
co-cultivation of B. japonicum M-8 with A. brasilense. Their use is determined by the fact
that yeast is rich in proteins as their content can reach 66%, with 10% of essential amino
acids. In addition, yeasts is also a source of growth factors, including vitamins.
Our studies indicate that all investigated concentrations were effective except of 1.0
g/dm3 and 10.0 g/dm3 (Table 4) at co-cultivation of rhizobia with A. brasilense 18-2.
Co-cultivation of B. japonicum M-8 with A. brasilense 410 in medium with glucose
concentrations ranging from 3.0 g/dm3 to 8.0 g/dm3 had not affected the number of rhizobia,
which have stayed within the error. Thus, the economically justified dose of dry yeast was 3
g/dm3.
Magnesium is required by rhizobia in small amounts for maintenance of bacteria
structure and functioning of enzyme systems in microorganism cells. Magnesium – is an
essential component of the enzyme ATP-dependent biochemical reactions. It is involved in
the metabolism of carbohydrates, proteins and fats, as well as redox reactions. Magnesium
activates glycolytic enzymes, enzymes cellular oxidation enzymes synthesis of nucleic acids.
Effect of different concentrations of MgSO4 × 7H2O in nutrient medium on growth activity of
diazotrophs was also studied.
Table 4. Effect of dry yeasts on growth of B. japonicum and A. brasilense
in liquid nutrient medium at their co-cultivation
Concentrations,
g/dm3
Number of bacteria, billion cells / cm3
B. japonicum
М-8
A. brasilense
18-2
B. japonicum
М-8
A. brasilense
410
1,0 3,5 ± 0,15 3,4 ± 0,15 2,7 ± 0,15 3,2 ± 0,12
2,0 3,8 ± 0,12 3,5 ± 0,20 3,3 ± 0,09 3,4 ± 0,15
3,0 3,6 ± 0,21 3,6 ± 0,18 3,4 ± 0,08 3,5 ± 0,12
4,0 3,7 ± 0,23 3,3 ± 0,18 3,6 ± 0,12 3,5 ± 0,17
5,0 3,7 ± 0,15 3,5 ± 0,21 3,5 ± 0,18 3,7 ± 0,15
6,0 3,7 ± 0,20 3,5 ± 0,12 3,5 ± 0,27 3,3 ± 0,15
7,0 3,6 ± 0,22 3,5 ± 0,21 3,5 ± 0,12 3,1 ± 0,15
8,0 3,6 ± 0,15 3,3 ± 0,21 3,4 ± 0,20 3,1 ± 0,23
9,0 3,7 ± 0,20 3,3 ± 0,17 3,3 ± 0,17 2,8 ± 0,15
10,0 3,3 ± 0,15 3,4 ± 0,18 3,3 ± 0,15 2,6 ± 0,15
7
The data presented in Table 5 indicate that the highest number of B. japonicum M-8 co-
cultivated with A. brasilense 18-2 was observed in variants with MgSO4 × 7H2O
concentration ranging from 0.1 g/dm3 to 0.5 g/dm3.
Table 5. Effect of MgSO4 × 7H2O on growth of B. japonicum and A. brasilense
in liquid nutrient medium at their co-cultivation
Concentrations,
g/dm3
Number of bacteria, billion cells / cm3
B. japonicum
М-8
A. brasilense
18-2
B. japonicum
М-8
A. brasilense
410
0,01 2,8 ± 0,15 3,3 ± 0,15 2,7 ± 0,17 2,8 ± 0,24
0,05 2,9 ± 0,12 3,6 ± 0, 21 2,6 ± 0,18 3,4 ± 0,17
0,1 3,6 ± 0,15 3,4 ± 0,23 3,3 ± 0,12 3,6 ± 0,09
0,2 3,5 ± 0,20 3,6 ± 0,20 3,5 ± 0,18 3,7 ± 0,15
0,3 3,3 ± 0,18 3,5 ± 0,23 3,5 ± 0,21 3,5 ± 0,15
0,4 3,4 ± 0,18 3,4 ± 0,15 3,3 ± 0,12 3,5 ± 0,15
0,5 3,3 ± 0,29 3,1 ± 0,27 2,7 ± 0,15 3,3 ± 0,17
It should be noted that use of given component in an amount of 0.1 g/dm3 is
reasonable, as it increases the titer of rhizobia the most. The same concentration of MgSO4 ×
7H2O is recommended for use at co-cultivation of B. japonicum M-8 with A. brasilense 410
since the increasing magnesium content in the medium from 0.1 g/dm3 to 0.4 g/dm3 the
number of bacteria ad varied but remained within the error.
As a result of our studies the various concentrations of nutrients sources that ensure the
maximum growth effect with minimum reasonable nutrients content in the medium for the
co-cultivation of soybean rhizobia with azospirills were identified. Data obtained can be used
as the basis for optimization of culture media for co-cultivation of B. japonicum and A.
brasilense.
|
| id | oai:ojs2.smic.in.ua:article-185 |
| institution | Agriciltural microbiology |
| keywords_txt_mv | keywords |
| language | English Ukrainian |
| last_indexed | 2026-07-23T01:06:03Z |
| publishDate | 2014 |
| publisher | Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine |
| record_format | ojs |
| resource_txt_mv | smicinua/95/bad3ed03f808b9ab6bfba77ca0227c95.pdf |
| spelling | oai:ojs2.smic.in.ua:article-1852026-07-22T10:10:44Z THE EFFECT OF NUTRIENT SOURCES ON GROWTH OF BRADYRHIZOBIUM JAPONICUM AND AZOSPIRILLUM BRASILENSE AT CO-CULTIVATION ВПЛИВ ДЖЕРЕЛ БІОГЕННИХ ЕЛЕМЕНТІВ НА РІСТ BRADYRHIZOBIUM JAPONICUM І AZOSPIRILLUM BRASILENSE ЗА ЇХ СУМІСНОГО КУЛЬТИВУВАННЯ Козар, С. Ф. Bradyrhizobium japonicum, Azospirillum brasilense, growth, nutrients, cultural medium, cultivation Bradyrhizobium japonicum, Azospirillum brasilense, ріст, біогенні елементи, середовище, культивування The paper presents the study of nutrient sources influence on the growth of Bradyrhizobium japonicum and Azospirillum brasilense at their co-cultivation. It was shown that at cultivation of diazotrophs in nutrient media with different concentrations of its components, soybean rhizobia had uneven growth activity. Glucose and molasses was observed to have the greatest impact on the rhizobia count. The results obtained are used as the basis for the optimization of culture medium for co-cultivation of rhizobia and azospirills. Досліджено вплив джерел біогенних елементів на ріст Bradyrhizobium japonicum і Azospirillum brasilense за їх сумісного культивування. Показано, що за вирощування діазотрофів у живильних середовищах із різними концентраціями їх складових бульбочкові бактерій сої мали неоднакову ростову активність. Найбільший вплив на чисельність ризобій мали глюкоза і меляса. Отримані результати досліджень є основою для оптимізації живильних середовищ для сумісного культивування ризобій і азоспірил. Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine 2014-03-31 Article Article Рецензована Стаття application/pdf application/pdf https://smic.in.ua/index.php/journal/article/view/185 10.35868/1997-3004.18.75-86 Agricultural microbiology; Vol. 18 (2014): Agriciltural microbiology; 75-86 Сільськогосподарська мікробіологія; Том 18 (2014): Сільськогосподарська мікробіологія; 75-86 1997-3004 10.35868/1997-3004.18 en uk https://smic.in.ua/index.php/journal/article/view/185/251 https://smic.in.ua/index.php/journal/article/view/185/252 Авторське право (c) 2014 S. F. Kozar https://creativecommons.org/licenses/by/4.0 |
| spellingShingle | Bradyrhizobium japonicum Azospirillum brasilense ріст біогенні елементи середовище культивування Козар, С. Ф. ВПЛИВ ДЖЕРЕЛ БІОГЕННИХ ЕЛЕМЕНТІВ НА РІСТ BRADYRHIZOBIUM JAPONICUM І AZOSPIRILLUM BRASILENSE ЗА ЇХ СУМІСНОГО КУЛЬТИВУВАННЯ |
| title | ВПЛИВ ДЖЕРЕЛ БІОГЕННИХ ЕЛЕМЕНТІВ НА РІСТ BRADYRHIZOBIUM JAPONICUM І AZOSPIRILLUM BRASILENSE ЗА ЇХ СУМІСНОГО КУЛЬТИВУВАННЯ |
| title_alt | THE EFFECT OF NUTRIENT SOURCES ON GROWTH OF BRADYRHIZOBIUM JAPONICUM AND AZOSPIRILLUM BRASILENSE AT CO-CULTIVATION |
| title_full | ВПЛИВ ДЖЕРЕЛ БІОГЕННИХ ЕЛЕМЕНТІВ НА РІСТ BRADYRHIZOBIUM JAPONICUM І AZOSPIRILLUM BRASILENSE ЗА ЇХ СУМІСНОГО КУЛЬТИВУВАННЯ |
| title_fullStr | ВПЛИВ ДЖЕРЕЛ БІОГЕННИХ ЕЛЕМЕНТІВ НА РІСТ BRADYRHIZOBIUM JAPONICUM І AZOSPIRILLUM BRASILENSE ЗА ЇХ СУМІСНОГО КУЛЬТИВУВАННЯ |
| title_full_unstemmed | ВПЛИВ ДЖЕРЕЛ БІОГЕННИХ ЕЛЕМЕНТІВ НА РІСТ BRADYRHIZOBIUM JAPONICUM І AZOSPIRILLUM BRASILENSE ЗА ЇХ СУМІСНОГО КУЛЬТИВУВАННЯ |
| title_short | ВПЛИВ ДЖЕРЕЛ БІОГЕННИХ ЕЛЕМЕНТІВ НА РІСТ BRADYRHIZOBIUM JAPONICUM І AZOSPIRILLUM BRASILENSE ЗА ЇХ СУМІСНОГО КУЛЬТИВУВАННЯ |
| title_sort | вплив джерел біогенних елементів на ріст bradyrhizobium japonicum і azospirillum brasilense за їх сумісного культивування |
| topic | Bradyrhizobium japonicum Azospirillum brasilense ріст біогенні елементи середовище культивування |
| topic_facet | Bradyrhizobium japonicum Azospirillum brasilense growth nutrients cultural medium cultivation Bradyrhizobium japonicum Azospirillum brasilense ріст біогенні елементи середовище культивування |
| url | https://smic.in.ua/index.php/journal/article/view/185 |
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