ЕПІФІТНА МІКРОФЛОРА РОСЛИН РИСУ (ORYZA SATIVA L.) ЯК ДЖЕРЕЛО ШТАМІВ З АГРОНОМІЧНО КОРИСНИМИ ДЛЯ РОСЛИН ВЛАСТИВОСТЯМИ
The paper covers the study of morphological, physiological and biochemical properties of associative microorganisms isolated from rice roots and plants’ response on inoculation. The agronomically useful properties, such as growth stimulation, ability to fix nitrogen and mobilize phosphates were obse...
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| Datum: | 2014 |
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| Format: | Artikel |
| Sprache: | Englisch Ukrainisch |
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Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine
2014
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Agriciltural microbiology| _version_ | 1871465695394398208 |
|---|---|
| author | Якубовська, А. І. |
| author_facet | Якубовська, А. І. |
| author_institution_txt_mv | [
{
"author": "А. І. Якубовська",
"institution": "Інститут сільського господарства Криму НААН"
}
] |
| author_sort | Якубовська, А. І. |
| baseUrl_str | https://smic.in.ua/index.php/journal/oai |
| collection | OJS |
| datestamp_date | 2026-07-22T10:10:44Z |
| description | The paper covers the study of morphological, physiological and biochemical properties of associative microorganisms isolated from rice roots and plants’ response on inoculation. The agronomically useful properties, such as growth stimulation, ability to fix nitrogen and mobilize phosphates were observed. The positive influence of isolated bacteria on growth and development of rice plants was observed. |
| doi_str_mv | 10.35868/1997-3004.18.100-108 |
| first_indexed | 2025-07-17T12:24:21Z |
| format | Article |
| fulltext |
EPIPHYTIC MICROFLORA OF RICE (ORYZA SATIVA L.) AS A SOURCE OF STRAINS
WITH AGRONOMICALLY USEFUL PLANT PROPERTIES
Yakubovskay A. I.
Institute of Agriculture Crimea NAAS, Department of Microbiology
107 Karl Marks str., Simferopol region, Gvardeyskoe 97513, Ukraine
E-mail: midyza_kiss@mail.ru
The paper covers the study of morphological, physiological and biochemical properties of
associative microorganisms isolated from rice roots and plants’ response on inoculation. The
agronomically useful properties, such as growth stimulation, ability to fix nitrogen and mobilize
phosphates were observed. The positive influence of isolated bacteria on growth and development
of rice plants was observed.
Key words: epiphytic microflora, associative microorganisms, rice.
Formation of soil fertility is influenced by a complex of natural and anthropogenic factors,
among which the most important is the vital activity of soil microorganisms [1–4]. Microorganisms
ensure the biological cycle of substances in nature, are involved in the transformation of mineral
and organic compounds, synthesize biologically active substances, affect physical and chemical
properties of the soil and regulate plant growth. Composition and number of microbial associations
in soils agrocoenoses determines the biological buffering of soils under the adverse influence of
various technological factors.
Rice cultivation cause significant problems related to the soil conditions. Thus, in order to
obtain high yields of rice the intensive technologies are used, including high yield potential
varieties, mineral and organic fertilizers, chemical pesticides against diseases, pests and segetal
plants). In some cases the crop is grown for 2–3 years in a row or in monoculture that results in
increasing doses of mineral fertilizers [7]. Besides, since the irrigation of rice in Ukraine is
performed by means of short flooding (water layer is missing at the beginning and end of the
growing season), that creates the optimal conditions for the accumulation of pathogens and
therefore increase of pesticides application leading to environmental pollution. Researchers from
different countries have established that prolonged flooding of rice fields decreases humus content
in soil, followed by waterlogging and salinization, which also reduces soil fertility, affects the
hydrolytic treatment of surrounding land and bays [8, 9].
One of the possible optimization ways of rice cultivation technology is use of microbial
preparations which stimulate development of useful forms of microorganisms that can compete with
the indigenous microbiota and dominate in plants rhizosphere [5, 6]. At this the promising area of
research, related to the selection of agronomically active strains is seemed to be the cultivation of
isolates of microbial communities of epiphytic microbiota. Epiphytes could be more adapted to
different plant species and be more efficient while using them as the basis for biological
preparations.
The aim of current research was to isolate active strains of epiphytic bacteria capable to
colonize the roots of rice and form effective associations with plants.
Objects and research methods. Given research was performed on roots of rice plants grown
on meadow – chestnut soil, selected from checks where rice was cultivated in monoculture for 10
years and microorganisms isolated from the aerial roots.
Isolation of associative microorganisms was performed using advanced method proposed by
Sherstoboyev M.K., Melnychuk T.M. and Melnyk L.I. [11]. Roots, free of soil and populated with
associative microorganisms, were obtained in sterile vegetation experiment. Roots were removed,
washed and, after a series of dilutions, solution was surface plated in Petri dishes on agar medium
cabbage medium # 19 [12]. The plates were incubated at 28 °C. Colony forming units (CFU) were
count at 3rd and 5th days. Abundance and occurrence frequency of morphotypes was calculated by
the method of qualitative determining of rhizosphere microorganisms [13].
Agronomically useful properties of selected bacteria were studied on Vynogradsky medium
without nitrogen (nitrogen fixation ability) and Muromtsev medium – to determine the ability of
microorganisms to dissolve soluble phosphates. Phytotoxicity of bacteria was studied using the
biological assay on wheat seeds [14]. The presence of phytotoxins in the bacterial culture was
determined by its influence on plants growth taking into the account the percentage of sprouted
seeds and measuring plants seedling length (embryonic shoot and roots).
To study the effect of isolates on rice plants the flooding and drying of the soil was
simulated close to the real field conditions. Later, the length of roots and shoots as well as their air-
dry mass were determined. Experiments planning and statistical analysis of data were performed by
the Dospekhov B. A. [15].
Results and discussion. Among the thirteen isolated morphotypes of bacteria from epiphytic
mikrobocoenosis on aerial roots of rice, five, which number was at least 10% of the total bacteria
count, appeared to be dominant (Table 1).
Table 1. Abundance of morphotypes of bacteria, isolated from air roots of rice
## of
isolate Appearance of colonies, medium #19 Occurrence
frequency, %
Abundance,
%
1
Rounded, 2–3 mm in diameter, smooth surface, even
edges, darker in the middle, rough rim of gray-blue
color, shiny, flat
10 2.6
2 Rounded, 5–7 mm in diameter, smooth surface, convex,
shiny, even edges, off-white or transparent 100 34.4
3 Rounded, 2–4 mm in diameter, smooth surface, even
edges, shiny, flat, orange 80 10.6
4 Rounded, 1–1.5 mm in diameter, even edges, smooth
surface, convex, shiny, white 50 16.6
5 Rounded, 5 mm in diameter, smooth surface, even
edges, convex with white rims 50 3.6
6 Rounded, 1–5 mm in diameter, smooth surface, even
edges, flat, mat, white 50 3.6
7 Rhizoidal shape, 7–8 mm in diameter, smooth surface,
flat, shiny, with a clear round midst 10 1.0
8 Rounded, 2 mm in diameter, smooth surface, even
edges, shiny, convex, concave from the middle, white 10 0.8
9 Rhizoidal shape, 3 mm in diameter, mat, flat, cream 10 1.4
10 Irregular shape, 8–10 mm in diameter, flat, mat, rough
surface, orange 30 13.4
11 Rounded, 5 mm in diameter, smooth surface, even
edges, convex, shiny, pale pink 10 0.6
12 Rounded, smooth surface, even edges, flat, shiny,
bright-red 10 0.2
13 Rounded, convex, shiny, white with pink roller on the
edge, 2–3 mm in diameter, even edges 10 10.3
Morphotypes of seven representatives out of thirteen (53.5%) were capable of nitrogen
fixation, whereas intensive growth was observed only in two cultures. 92.3% of isolates have shown
growth of various intensity on the Muromtsev cultural media. Among the studied isolates seven
were capable of nitrogen fixation and phosphorous mobilization.
As is known, many rhizobia produce physiologically active substances that can stimulate or
inhibit plants growth and development. It was shown that isolated bacteria had promoted
germination parameters in wheat test-culture by 7.7–30.8% as compared to control (Table 2).
Table 2. Influence of bacterial isolates on wheat seed germination parameters
Variants
Number of
germinated
seeds,
pcs.*
Seeds
germination, % Increment to control, %
Control 13.0 65 -
Isolates
ARR 1 16.0 80 23.1
ARR 2 16.0 80 23.1
ARR 3 15.0 75 15.4
ARR 5 17.0 85 30.8
ARR 6 15.0 75 15.4
ARR 7 17.0 85 30.8
ARR 9 17.0 85 30.8
ARR 10 15.0 75 15.4
ARR 11 16.0 80 23.1
ARR 13 14.0 70 7.7
LSD05 2.81 - -
Eight isolates were capable to stimulate root growth, four of which had also stimulated
growth of wheat seedlings. Three isolates had enhanced development of both roots (increase of
length by 31.5%, 20.8% and 32.6% to control) and wheat germs (respectively 6.4%, 4.4% and
6.4%) (fig. 1).
Figure 1. Influence isolates the length of roots and shoots of wheat seeds
1 – control; 2 – ARR 1; 3 – ARR 2; 4 – ARR 3; 5 – ARR 5; 6 – ARR 6; 7 – ARR 7;
8 – ARR 9; 10 – ARR 10; 11 – ARR 11; 12 – ARR 13.
1 2 3 4 5 6 7 8 9 10 11
length of roots, mm
length of seedling, mm
Isolates effect on the development of rice plants was studied in the green house experiment.
The slight increase of seedlings length in the variant with of seeds inoculation with ARR 7 isolate
by 1.5% was observed. In variant with ARR 3 isolate the 30.3% increase of roots length and 5.4%
increment of their air-dry weight comparing to the control was noticed.
According to the primary results the three isolates: ARR 3, ARR 6, ARR 7 were selected for
the subsequent study in the field conditions. ARR 7 isolate was obtained from the colonies of
orange color, irregular shape, diameter 8 – 10 mm, flat, matte, with a rough surface, frequency of
occurrence – 13.4% (Fig. 2). Occurrence frequency of other bacteria morphotypes that formed
round colonies, 1 – 5 mm in diameter, with smooth surface, level edges, flat, dull and white – was
50% (selected isolate ARR 3) (Fig. 2). At cultivation on cabbage agar, the high growth rate of these
morphotypes was observed.
Figure 2. Morphotypes of epiphytic microbiota of rice air-roots
(1 – ARR 7; 2 – ARR 3)
Thus, the initial study of strains efficiency had revealed that isolates had promoted seeds
germination, but in different degrees (from 7.7% to 30.8%, compared to control). Eight isolates
were able to stimulate root growth of wheat seeds, and four – to promote growth of wheat seedlings.
Isolates ARR 6, ARR 7, ARR 10 were able to stimulate growth of both roots and shoots.
Among the studied bacteria three isolates with the most distinguished agronomically useful
properties (growth stimulation, nitrogen fixation, mobilization of phosphorous) were selected for
further research.
1 2
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| id | oai:ojs2.smic.in.ua:article-187 |
| institution | Agriciltural microbiology |
| keywords_txt_mv | keywords |
| language | English Ukrainian |
| last_indexed | 2026-07-23T01:06:07Z |
| publishDate | 2014 |
| publisher | Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine |
| record_format | ojs |
| resource_txt_mv | smicinua/1c/a08d199b5a1cccf9607d70fea4419a1c.pdf |
| spelling | oai:ojs2.smic.in.ua:article-1872026-07-22T10:10:44Z EPIPHYTIC MICROFLORA OF RICE (ORYZA SATIVA L.) AS A SOURCE OF STRAINS WITH AGRONOMICALLY USEFUL PLANT PROPERTIES ЕПІФІТНА МІКРОФЛОРА РОСЛИН РИСУ (ORYZA SATIVA L.) ЯК ДЖЕРЕЛО ШТАМІВ З АГРОНОМІЧНО КОРИСНИМИ ДЛЯ РОСЛИН ВЛАСТИВОСТЯМИ Якубовська, А. І. epiphytic microflora, associative microorganisms, rice епіфітна мікрофлора, асоціативні мікроорганізми, рослини рису The paper covers the study of morphological, physiological and biochemical properties of associative microorganisms isolated from rice roots and plants’ response on inoculation. The agronomically useful properties, such as growth stimulation, ability to fix nitrogen and mobilize phosphates were observed. The positive influence of isolated bacteria on growth and development of rice plants was observed. Проведено виділення асоціативних мікроорганізмів з коренів рису. Досліджено морфологічні і фізіолого-біохімічні властивості ізолятів та реакцію рослин на інокуляцію. Показано наявність у ізолятів агрономічно корисних властивостей: рістстимуляції, азотфіксації, фосфатмобілізації. Відмічено їх позитивний вплив на розвиток рослин рису. Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine 2014-03-31 Article Article Рецензована Стаття application/pdf application/pdf https://smic.in.ua/index.php/journal/article/view/187 10.35868/1997-3004.18.100-108 Agricultural microbiology; Vol. 18 (2014): Agriciltural microbiology; 100-108 Сільськогосподарська мікробіологія; Том 18 (2014): Сільськогосподарська мікробіологія; 100-108 1997-3004 10.35868/1997-3004.18 en uk https://smic.in.ua/index.php/journal/article/view/187/257 https://smic.in.ua/index.php/journal/article/view/187/258 Авторське право (c) 2014 A. I. Yakubovskay https://creativecommons.org/licenses/by/4.0 |
| spellingShingle | епіфітна мікрофлора асоціативні мікроорганізми рослини рису Якубовська, А. І. ЕПІФІТНА МІКРОФЛОРА РОСЛИН РИСУ (ORYZA SATIVA L.) ЯК ДЖЕРЕЛО ШТАМІВ З АГРОНОМІЧНО КОРИСНИМИ ДЛЯ РОСЛИН ВЛАСТИВОСТЯМИ |
| title | ЕПІФІТНА МІКРОФЛОРА РОСЛИН РИСУ (ORYZA SATIVA L.) ЯК ДЖЕРЕЛО ШТАМІВ З АГРОНОМІЧНО КОРИСНИМИ ДЛЯ РОСЛИН ВЛАСТИВОСТЯМИ |
| title_alt | EPIPHYTIC MICROFLORA OF RICE (ORYZA SATIVA L.) AS A SOURCE OF STRAINS WITH AGRONOMICALLY USEFUL PLANT PROPERTIES |
| title_full | ЕПІФІТНА МІКРОФЛОРА РОСЛИН РИСУ (ORYZA SATIVA L.) ЯК ДЖЕРЕЛО ШТАМІВ З АГРОНОМІЧНО КОРИСНИМИ ДЛЯ РОСЛИН ВЛАСТИВОСТЯМИ |
| title_fullStr | ЕПІФІТНА МІКРОФЛОРА РОСЛИН РИСУ (ORYZA SATIVA L.) ЯК ДЖЕРЕЛО ШТАМІВ З АГРОНОМІЧНО КОРИСНИМИ ДЛЯ РОСЛИН ВЛАСТИВОСТЯМИ |
| title_full_unstemmed | ЕПІФІТНА МІКРОФЛОРА РОСЛИН РИСУ (ORYZA SATIVA L.) ЯК ДЖЕРЕЛО ШТАМІВ З АГРОНОМІЧНО КОРИСНИМИ ДЛЯ РОСЛИН ВЛАСТИВОСТЯМИ |
| title_short | ЕПІФІТНА МІКРОФЛОРА РОСЛИН РИСУ (ORYZA SATIVA L.) ЯК ДЖЕРЕЛО ШТАМІВ З АГРОНОМІЧНО КОРИСНИМИ ДЛЯ РОСЛИН ВЛАСТИВОСТЯМИ |
| title_sort | епіфітна мікрофлора рослин рису (oryza sativa l.) як джерело штамів з агрономічно корисними для рослин властивостями |
| topic | епіфітна мікрофлора асоціативні мікроорганізми рослини рису |
| topic_facet | epiphytic microflora associative microorganisms rice епіфітна мікрофлора асоціативні мікроорганізми рослини рису |
| url | https://smic.in.ua/index.php/journal/article/view/187 |
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