XАРАКТЕР ЖИТТЄДІЯЛьНОСТІ rhizobium RADIOBACTER НА ПОВЕРхНІ НАСІННЯ І В ЗОНІ КОРІННЯ РОСЛИН ПШЕНИЦІ ОЗИМОЇ
Preservation of Rhizobium radiobacter strains 599, 1333, 5006 and 5718 on inoculated seeds and further development in the rhizosphere soil of winter wheat plants of Poliska 90 variety was observed. It was found that bacterial cells R. radiobacter 599, 1333, 5006 and 5718 can retain on bacterized see...
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| Datum: | 2013 |
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Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine
2013
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Agriciltural microbiology| _version_ | 1871465713949999104 |
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| author | Трепач , А.О. |
| author_facet | Трепач , А.О. |
| author_institution_txt_mv | [
{
"author": "А.О. Трепач ",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
}
] |
| author_sort | Трепач , А.О. |
| baseUrl_str | https://smic.in.ua/index.php/journal/oai |
| collection | OJS |
| datestamp_date | 2026-07-22T10:10:44Z |
| description | Preservation of Rhizobium radiobacter strains 599, 1333, 5006 and 5718 on inoculated seeds and further development in the rhizosphere soil of winter wheat plants of Poliska 90 variety was observed. It was found that bacterial cells R. radiobacter 599, 1333, 5006 and 5718 can retain on bacterized seeds of winter wheat for two months and can ecize in the root zone of plants. |
| doi_str_mv | 10.35868/1997-3004.17.31-38 |
| first_indexed | 2025-07-17T12:24:24Z |
| format | Article |
| fulltext |
NATURE OF RHIZOBIUM RADIOBACTER LIFE ACTIVITY ON THE SEEDS
SURFACE AND IN ROOT ZONE OF WINTER WHEAT PLANTS
Trepach A. O.
Institute of Agricultural Microbiology and Agro-Industrial Manufacture NAAS of
Ukraine,
97, Shevchenko Str., Chernihiv, 14027, Ukraine
E-mail: trepach@e-mail.ua
Preservation of Rhizobium radiobacter strains 599, 1333, 5006 and 5718 on
inoculated seeds and further development in the rhizosphere soil of winter wheat plants of
Poliska 90 variety was observed. It was found that bacterial cells R. radiobacter 599,
1333, 5006 and 5718 can retain on bacterized seeds of winter wheat for two months and
can ecize in the root zone of plants.
Keywords: Rhizobium radiobacter, winter wheat.
Availability of phosphorus is one of the key factors for plant growth and
development. However, due to the low solubility of soil phosphorus, cultivated plants
experience shortage of this element [1 – 3]. Therefore, optimization of phosphorus
nutrition of agricultural crops plays a significant role in improvement of plants
productivity. In particular, this applies to winter wheat, which has a low capacity to uptake
phosphorus from the soil and is particularly sensitive to its deficit during early stages of
organogenesis, when plants phosphorus supply is essential to their growth, development
and yield formation [4 – 6].
Application of phosphorous mobilizing bacteria and microbial preparations on their
basis in modern technologies of winter wheat cultivation can be an effective mean of crop
productivity enhancement especially taking into the account the undeniable importance of
phosphorus as one of the most important elements of plant nutrition.
Efficacy of phosphorous mobilizing bacteria in crop production technologies is
determined in great extent by their ability to ecize in the plants root zone. Basically,
survival of bacteria in the root zone of plants depends on their ability to colonize a
particular area of root system and compete for it with other microorganisms [7]. Therefore,
influence on the activity and orientation of plant-microbe interactions is only possible
under the thorough study of bacteria competitiveness upon their introduction to the root
zone of plants, their impact on the functioning of the existing microbial communities and
environmental influence on its activity [8].
Given study is aimed to examine the nature of Rhizobium radiobacter life activity
on seeds surface and in winter wheat roots area.
Materials and methods. Experiments were performed with Rhizobium radiobacter
bacteria (strains 599, 1333, 5006, 5718), isolated by L.M. Tokmakova from the root zone
of cultured plants as active phosphorous mobilizing bacteria (Collection of useful soil
microorganisms of the Institute of Agricultural Microbiology and Agro-Industrial
Manufacture NAAS). Paenibacillus polymyxa KB phosphorous mobilizing bacteria –
biological agents of Polymyxobakteryn microbial preparation [9], which is recommended
for application in growing technology of some cultured crops, were used as a positive
control.
Preservation of bacterial cells on bacterized seeds of winter wheat was controlled by
wiping-off bacteria from seeds’ surface, their subsequent inoculation on meat-peptone
agar (MPA) and record using hemocytometer (Goryaev's chamber) [10]. Experiments
were conducted on the first day of seeds bacterization and monthly for 150 days.
Ecize of R. radiobacter bacteria in the rhizosphere of winter wheat plants of Poliska
90 variety was studied in small pot experiments on leached light loam meadow-chernozem
soils (humus – 2.12%, easily hydrolyzed nitrogen – 95.2 mg / kg, phosphorus – 226 mg /
kg, 108 mg / kg of exchangeable potassium, pHsalt = 5.30) on the experimental fields of
the Institute of Agricultural Microbiology and Agro-Industrial Manufacture NAAS. The
total area of experiment was 92.2 sq.m, repetition of the experiment – four-fold.
Scheme of the experiment with winter wheat (Poliska 90 variety):
1. Without bacterization (control).
Bacterization:
2. Paenibacillus polymyxa KB.
3. Rhizobium radiobacter 599.
4. R. radiobacter 1333.
5. R. radiobacter 5006.
6. R. radiobacter 5718.
Inoculation of winter wheat seeds was performed with the suspension of bacterial
cells of studied phosphorous mobilizing strains at the rate of 0.5 million cells per seed in
accordance to SOU 01.11-37-782 [11]. The labeled strains of bacteria R. radiobacter,
adapted to streptomycin by the Zibalskiy method [12] were used in the studies.
Record of streptomycin resistant bacteria in the root zone of winter wheat plants was
carried out in the tillering (at autumn), booting and heading stages. Bacteria were counted
using corresponding culture dilutions method on nutrient media MPA, containing 2 g / l of
streptomycin with sodium salt of levorin (for inhibition of micromycetes growth) using
method of antibiotic resistant variants [13]. Seeds without bacterization were used as
control keeping the record number of indigenous bacteria resistant to streptomycin.
Small pot experiments were planned and conducted in accordance to Dospehov
[14].
Results and discussion. It was established that within the first month after
bacterization there were at least 200 thousand bacterial cells persisted on the seed surface
(Fig. 1). R. radiobacter 599 and R. radiobacter 5006 had most number of the bacteria
preserved, 240 thous. / seed and 200 thous. / seed, correspondingly. Within two months
more than 100 thous. of bacterial cells / seed have remained on seeds. Subsequently, the
number of bacterial cells of R. radiobacter on seed have decreased. Nevertheless, the life
period of the increased number of inoculant cells on the seeds should be sufficient for
development and naturalization of bacteria in root zone of plants.
The results obtained have demonstrated that a large number of bacterial cells of R.
radiobacter had preserved on winter wheat seeds for two months.
0
100
200
300
400
500
600
inoculation
day
1 month 2 month 3 month 4 month 5 month
Exposition
N
um
be
r
of
b
ac
te
ri
al
c
el
ls
, t
ho
us
. /
se
ed
P. polymyxa KB R. radiobacter 599
R. radiobacter 1333 R. radiobacter 5006
R. radiobacter 5718
FIGURE 1. Preservation dynamics of phosphorous mobilizing bacteria cells on bacterized
seeds of winter wheat (Poliska 90 variety).
Assumption on the direct and indirect positive effects of introduced bacteria on
plants can be made only if inoculants ecize and develop in the root zone of plants. This
caused the examination of the ecize rates of R. radiobacter in the root zone of inoculated
winter wheat plants.
The results obtained have demonstrated that a large number of bacterial cells of R.
radiobacter had preserved on winter wheat seeds for two months.
Assumption on the direct and indirect positive effects of introduced bacteria on
plants can be made only if inoculants ecize and develop in the root zone of plants. This
caused the examination of the ecize rates of R. radiobacter in the root zone of inoculated
winter wheat plants.
It was shown that rhizosphere soil of bacterized winter wheat plants has many
streptomycin resistant bacteria, especially at tillering stage – up to 1021 thous. / g soil (in
variant with R. radiobacter 1333 strain) (Fig. 2). During later organogenesis stages –
booting and heading, the number of inoculant cells in the root zone of plants had gradually
decreased and was 559 thous. / g soil and 239 thous. / g soil, respectively. At the same
time, the number of bacteria naturally resistant to streptomycin was significantly lower
and had slightly varied during the study (219 – 233 thous. / g soil).
It was shown that by the number of streptomycin resistant bacteria, the variants with
plants inoculated with R. radiobacter had not inferior variant of P. polymyxa KB.
22
1
23
3
21
9
25
0
54
0
10
30
20
9
51
0
98
7
21
1
52
1
10
21
23
9
55
9
98
4
22
2
50
1
10
09
0
200
400
600
800
1000
1200
Tillering Booting Heading
Plant development stage
N
um
be
r
of
b
ac
te
ri
a,
th
ou
s.
/ g
o
f s
oi
l
W/o inoculation P. polymyxa KB R. radiobacter 599
R. radiobacter 1333 R. radiobacter 5006 R. radiobacter 5718
FIGURE. 2. Number of streptomycin resistant bacteria in the root zone of winter wheat
plants (Poliska 90 variety)
The high number of introduced bacterial cells in the root zone of winter wheat
plants is a very important index, since it is known, that during the early period of crop
vegetation wheat plants needs for phosphorus is particularly high, with its absorption rate
per unit of dry weight higher than at later developmental stages [15]. Despite the relative
decrease of introduced microorganisms’ number, high initial bacterial pool improves plant
growth conditions by both dissolving hard soil phosphates [16] and production of
phytohormones [17].
Therefore, the period of active phosphorus uptake of by plants is consistent with
active development of phosphorous mobilizing bacteria introduced into the root zone of
winter wheat plants, indicating a high agronomic value of seeds bacterization with R.
radiobacter.
Thus, it was established that phosphorous mobilizing bacteria R. radiobacter can
preserve on bacterized seeds for two months with the bacterial count of more than 100
thousand cells / seed and can ecize and develop in the root zone of winter wheat plants.
The results obtained may might be interesting not only from the scientific point of view
but also for the practical application of studied microorganisms in the technologies of
winter wheat cultivation. In addition to the bacteria impact on the production process of
winter wheat crop, the ability of bacterial cells to persist on seeds surface for two months
is of a big importance creating favorable conditions for pre-sowing seeds inoculation.
|
| id | oai:ojs2.smic.in.ua:article-196 |
| institution | Agriciltural microbiology |
| keywords_txt_mv | keywords |
| language | English Ukrainian |
| last_indexed | 2026-07-23T01:06:25Z |
| publishDate | 2013 |
| publisher | Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine |
| record_format | ojs |
| resource_txt_mv | smicinua/46/b77bfdbbf072e571faf491bb7d8d2146.pdf |
| spelling | oai:ojs2.smic.in.ua:article-1962026-07-22T10:10:44Z NATURE OF RHIZOBIUM RADIOBACTER LIFE ACTIVITY ON THE SEEDS SURFACE AND IN ROOT ZONE OF WINTER WHEAT PLANTS XАРАКТЕР ЖИТТЄДІЯЛьНОСТІ rhizobium RADIOBACTER НА ПОВЕРхНІ НАСІННЯ І В ЗОНІ КОРІННЯ РОСЛИН ПШЕНИЦІ ОЗИМОЇ Трепач , А.О. Rhizobium radiobacter, winter wheat Rhizobium radiobacter, пшениця озима Preservation of Rhizobium radiobacter strains 599, 1333, 5006 and 5718 on inoculated seeds and further development in the rhizosphere soil of winter wheat plants of Poliska 90 variety was observed. It was found that bacterial cells R. radiobacter 599, 1333, 5006 and 5718 can retain on bacterized seeds of winter wheat for two months and can ecize in the root zone of plants. у дослідженнях з пшеницею озимою сорту Поліська 90 показано можливість збереження клітин штамів бактерій Rhizobium radiobacter 599, 1333, 5006, 5718 на бактеризованому насінні та їх розвитку у ризосферному ґрунті рослин. Встановлено, що клітини бактерій R. radiobacter 599, 1333, 5006, 5718 зберігаються на бактеризованому насінні пшениці озимої протягом двох місяців та здатні приживатися у кореневій зоні рослин. Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine 2013-10-01 Article Article Рецензована Стаття application/pdf application/pdf https://smic.in.ua/index.php/journal/article/view/196 10.35868/1997-3004.17.31-38 Agricultural microbiology; Vol. 17 (2013): Agriciltural microbiology; 31-38 Сільськогосподарська мікробіологія; Том 17 (2013): Сільськогосподарська мікробіологія; 31-38 1997-3004 10.35868/1997-3004.17 en uk https://smic.in.ua/index.php/journal/article/view/196/273 https://smic.in.ua/index.php/journal/article/view/196/274 Авторське право (c) 2013 A. O. Trepach https://creativecommons.org/licenses/by/4.0 |
| spellingShingle | Rhizobium radiobacter пшениця озима Трепач , А.О. XАРАКТЕР ЖИТТЄДІЯЛьНОСТІ rhizobium RADIOBACTER НА ПОВЕРхНІ НАСІННЯ І В ЗОНІ КОРІННЯ РОСЛИН ПШЕНИЦІ ОЗИМОЇ |
| title | XАРАКТЕР ЖИТТЄДІЯЛьНОСТІ rhizobium RADIOBACTER НА ПОВЕРхНІ НАСІННЯ І В ЗОНІ КОРІННЯ РОСЛИН ПШЕНИЦІ ОЗИМОЇ |
| title_alt | NATURE OF RHIZOBIUM RADIOBACTER LIFE ACTIVITY ON THE SEEDS SURFACE AND IN ROOT ZONE OF WINTER WHEAT PLANTS |
| title_full | XАРАКТЕР ЖИТТЄДІЯЛьНОСТІ rhizobium RADIOBACTER НА ПОВЕРхНІ НАСІННЯ І В ЗОНІ КОРІННЯ РОСЛИН ПШЕНИЦІ ОЗИМОЇ |
| title_fullStr | XАРАКТЕР ЖИТТЄДІЯЛьНОСТІ rhizobium RADIOBACTER НА ПОВЕРхНІ НАСІННЯ І В ЗОНІ КОРІННЯ РОСЛИН ПШЕНИЦІ ОЗИМОЇ |
| title_full_unstemmed | XАРАКТЕР ЖИТТЄДІЯЛьНОСТІ rhizobium RADIOBACTER НА ПОВЕРхНІ НАСІННЯ І В ЗОНІ КОРІННЯ РОСЛИН ПШЕНИЦІ ОЗИМОЇ |
| title_short | XАРАКТЕР ЖИТТЄДІЯЛьНОСТІ rhizobium RADIOBACTER НА ПОВЕРхНІ НАСІННЯ І В ЗОНІ КОРІННЯ РОСЛИН ПШЕНИЦІ ОЗИМОЇ |
| title_sort | xарактер життєдіяльності rhizobium radiobacter на поверхні насіння і в зоні коріння рослин пшениці озимої |
| topic | Rhizobium radiobacter пшениця озима |
| topic_facet | Rhizobium radiobacter winter wheat Rhizobium radiobacter пшениця озима |
| url | https://smic.in.ua/index.php/journal/article/view/196 |
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