СТИМуЛюВАННЯ ПРОРОСТАННЯ НАСІННЯ ПЕЛюШКИ бІОПРЕПАРАТАМИ ВІТчИЗНЯНОгО ВИРОбНИЦТВА
The paper covers the research results of biological inoculation effect on field pea Pisum arvense seeds germination under different temperature regimes. The optimum temperatures ensuring high seeds germination rates were defined. It was established that Rhizohumin have showed the best results by the...
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| Date: | 2013 |
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Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine
2013
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| Journal Title: | Agriciltural microbiology |
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Agriciltural microbiology| _version_ | 1871465716078608384 |
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| author | Вишневська , О.В. Маркіна , О.В. |
| author_facet | Вишневська , О.В. Маркіна , О.В. |
| author_institution_txt_mv | [
{
"author": "О.В. Вишневська ",
"institution": "Інститут сільського господарства Полісся НААН"
},
{
"author": "О.В. Маркіна ",
"institution": "Інститут сільського господарства Полісся НААН"
}
] |
| author_sort | Вишневська , О.В. |
| baseUrl_str | https://smic.in.ua/index.php/journal/oai |
| collection | OJS |
| datestamp_date | 2026-07-22T10:10:44Z |
| description | The paper covers the research results of biological inoculation effect on field pea Pisum arvense seeds germination under different temperature regimes. The optimum temperatures ensuring high seeds germination rates were defined. It was established that Rhizohumin have showed the best results by the efficiency coefficient of biological preparation use on the early stages of plant development. |
| doi_str_mv | 10.35868/1997-3004.17.39-47 |
| first_indexed | 2025-07-17T12:24:24Z |
| format | Article |
| fulltext |
PROMOTION OF FIELD PEA PISUM ARVENSE SEEDS GERMINATION
WITH BIOLOGICAL PREPARATIONS OF DOMESTIC PRODUCTION
O. V. Vyshnevska, O. V. Markina
Institute of Agriculture of Polissya NAAS
131, Kyivse shosse, 10007, Zhytomyr, Ukraine
E-mail: oksanavish@rambler.ru
The paper covers the research results of biological inoculation effect on
field pea Pisum arvense seeds germination under different temperature regimes.
The optimum temperatures ensuring high seeds germination rates were defined. It
was established that Rhizohumin have showed the best results by the efficiency
coefficient of biological preparation use on the early stages of plant development.
Keywords: rhizohumin, azotofit, germination rate, germination.
Plants ontogenesis is taken place in close interaction with soil
microorganisms that colonize the rhizosphere and form "microorganisms – root
system" associations [4]. During its life activity this system is influenced by
various external biotic and abiotic factors, some of which are unfavorable to living
organisms. Therefore, improvement of ecosystem resistance to stressful conditions,
enhancement of the organisms’ protective functions with specific biological
methods could significantly reduce or prevent the influence of negative factors.
Thus, many authors have showed the versatile action of rhizosphere
microorganisms that enter into the compositions of biological inoculants [1, 2, 3, 4,
6].
However, the question of bacterial preparations influence on field pea seeds
germination, especially during the early stages of their interaction at different
temperatures remains poorly understood. Therefore, we studied the effect of
biological preparations on the seedlings development in the juvenile period of plant
growth at different temperature regimes.
Materials and methods. Experiments were conducted with the seeds of
field pea Pisum arvense (Zvyagelska variety) used for grains and biological
inoculants (Rhizohumin based on Rhizobium leguminosarum 31, Institute of
Agricultural Microbiology and Agricultural Production NAAS) and Azotofit
(based on Azotobacter chroococcum, "BTU Center"). The following parameters
were recorded: germination rate, germination capacity, root system and seedlings
growth rates depending on inoculation and temperature regimes.
Germination rates and germination capacity was determined in laboratory
using standard method [5]. Field pea Pisum arvense seeds germination rates were
determined after 72 hours. Root system and seedlings growth rates were measured
on the 4th, 5th, 6th and 7th day. Repitability of model experiments – three-fold.
Statistical processing of the experimental data was performed using standard
methods [7, 8].
Results and discussion. Laboratory studies had established that biological
preparations differently affect seeds germination rates depending on the
temperature. Thus, the optimum temperature for germination of field pea seeds was
15 °C and 20 °C. In this regime the highest seeds germination rate by the seventh
day of experiment was 80 – 94% (depending on the treatment (Fig. 1a). The
biggest number of germinated seeds – 92 – 94% at these temperatures was
recorded in variants with Rhizohumin, with maximum 94% – at 20 °C, that exceed
control values on 10 – 15%. Efficiency ratio of Rhizohumin to Azotofit for seeds
germination was 1.02 – 1.19 (Fig. 1b). It was established that both increase up to
24 °C and decrease of temperature to 10 °C have reduced the efficiency of
biological preparations as compared to the control on 9 – 24%.
During examination of seeds germination the germination rate index should
also be considered defining the growth rate. Thus, the highest germination rate of
field pea seeds was observed at 20 °C (50 – 62%) (Fig. 2a). Slightly lower
germination rates were recorded at 15 °C and 24 °C. Moreover, at 24 °C the
germination rates of seeds treated with different microbial preparations, did not
exceed control values. At 10 °C the percentage of seed germination was 20 – 32%,
which is 1.3 – 1.7 times lower when compared to the 15 °C and 24 °C temperature
regimes.
Rhizohumin have showed the advantages over the control and Azotofit by
the germination rate index (32 – 62%) in laboratory conditions at different
temperatures except for the variant with 24 °C temperature. At this, the efficiency
ratio of Rhizohumin to Azotofit for germination rate was 1.1 – 1.6.
Rhizohumin have also predominated in laboratory studies in terms of
development rates of main indicators of field pea seeds germination at different
temperatures (Fig. 3). Thus, seed treatment with Rhizohumin the number of
germinating seeds per day was in 1.1 – 2.1 times higher than in control, depending
on the temperature (Fig. 3a). Efficiency ratio of Rhizohumin to Azotofit by this
index was 1.1 – 1.6 depending on the temperature and development rates.
Correspondingly, the growth of roots of first order at Rhizohumin application was
more intense than in control plants (in 1.1 – 1.8 times). The efficiency ratio
between the studied preparations was 1.1 – 1.8, except for the variants with seeds
exposed to 24 °C temperature, where Azotofit had an advantage with a efficiency
ratio 1.1 – 2.5 depending on the development stage in dynamics (Fig. 3b).
Inoculated seedlings have exceeded control values not only by germinating
capacity and dynamics of growth processes, but also by root mass. Thus,
influenced by biological preparation the raw root weight was higher on 2 – 22%
when compared to control where the root mass was within the 0.023 – 0.043 g /
plant, depending on the temperature.
Similar growth peculiarities were also observed in study of vegetative mass
development in variants with Rhizohumin inoculation. Seedlings in this variant
were formed 5.43 – 18.0 mm long in up to 8 units (Fig. 4). The best conditions for
shoots formation were established at 20 °C with the growth dynamics 0.02 – 0.08
mm per day. Compared to control plants in variants with Rhizohumin inoculation
were developed better in 1.2 – 2.8 times. At this, the efficiency ratio of
Rhizohumin to Azotofit was 1.8 – 4.8 except for the variant with plants grown
under the 24 °C temperature.
FIGURE 1. Effect of temperature on field pea Pisum arvense seeds germination on the seventh day (a) and comparative analysis
of the biological inoculants impact (efficiency ratio) (b) on seeds germination based on the temperature
FIGURE 2. Effect of temperature on field pea Pisum arvense seeds germination rates (a) and comparative analysis of the
biological inoculants influence (efficiency ratio) (b) on seeds germination rates in different temperature regimes.
FIGURE.3. Germination and root development rates of field pea Pisum arvense,
depending on the biological preparation and temperature.
Note: a – number of germinated seeds,%; b – length of the roots of the
first order, mm
Hence, it was established that biological preparations based on the
rhizosphere bacteria have a positive effect on germination capacity and
germination rate of field pea Pisum arvense seeds, as well as on plants growth and
development in the early stages of their ontogenesis. It was shown that biological
preparations perform better under the 15 °C and 20 °C temperature conditions with
Rhizohumin predominating effect on seeds germination and seedlings growth
rates.
FIGURE 4. Vegetative mass accumulation rates of field pea Pisum arvense during
seeds germination, depending on the type of biological preparation
and temperature.
(a – number of seeds which formed shoots, %; b – shoots length, mm)
|
| id | oai:ojs2.smic.in.ua:article-197 |
| institution | Agriciltural microbiology |
| keywords_txt_mv | keywords |
| language | English Ukrainian |
| last_indexed | 2026-07-23T01:06:27Z |
| publishDate | 2013 |
| publisher | Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine |
| record_format | ojs |
| resource_txt_mv | smicinua/13/9574c325f8b83aa7fed74d5c05440d13.pdf |
| spelling | oai:ojs2.smic.in.ua:article-1972026-07-22T10:10:44Z PROMOTION OF FIELD PEA PISUM ARVENSE SEEDS GERMINATION WITH BIOLOGICAL PREPARATIONS OF DOMESTIC PRODUCTION СТИМуЛюВАННЯ ПРОРОСТАННЯ НАСІННЯ ПЕЛюШКИ бІОПРЕПАРАТАМИ ВІТчИЗНЯНОгО ВИРОбНИЦТВА Вишневська , О.В. Маркіна , О.В. rhizohumin, azotofit, germination rate, germination Ризогумін, Азотофіт, пелюшка, енергія проростання, схожість насіння The paper covers the research results of biological inoculation effect on field pea Pisum arvense seeds germination under different temperature regimes. The optimum temperatures ensuring high seeds germination rates were defined. It was established that Rhizohumin have showed the best results by the efficiency coefficient of biological preparation use on the early stages of plant development. Наведено результати досліджень впливу біоінокуляції за різних температурних режимів на процеси проростання насіння пелюшки. Визначено оптимальні температури для забезпечення високої енергії проростання, схожості насіння. Встановлено, що за коефіцієнтом ефективності використання біопрепаратів на початкових етапах розвитку рослин кращим є Ризогумін. Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine 2013-10-01 Article Article Рецензована Стаття application/pdf application/pdf https://smic.in.ua/index.php/journal/article/view/197 10.35868/1997-3004.17.39-47 Agricultural microbiology; Vol. 17 (2013): Agriciltural microbiology; 39-47 Сільськогосподарська мікробіологія; Том 17 (2013): Сільськогосподарська мікробіологія; 39-47 1997-3004 10.35868/1997-3004.17 en uk https://smic.in.ua/index.php/journal/article/view/197/275 https://smic.in.ua/index.php/journal/article/view/197/276 Авторське право (c) 2013 O. V. Vyshnevska, O. V. Markina https://creativecommons.org/licenses/by/4.0 |
| spellingShingle | Ризогумін Азотофіт пелюшка енергія проростання схожість насіння Вишневська , О.В. Маркіна , О.В. СТИМуЛюВАННЯ ПРОРОСТАННЯ НАСІННЯ ПЕЛюШКИ бІОПРЕПАРАТАМИ ВІТчИЗНЯНОгО ВИРОбНИЦТВА |
| title | СТИМуЛюВАННЯ ПРОРОСТАННЯ НАСІННЯ ПЕЛюШКИ бІОПРЕПАРАТАМИ ВІТчИЗНЯНОгО ВИРОбНИЦТВА |
| title_alt | PROMOTION OF FIELD PEA PISUM ARVENSE SEEDS GERMINATION WITH BIOLOGICAL PREPARATIONS OF DOMESTIC PRODUCTION |
| title_full | СТИМуЛюВАННЯ ПРОРОСТАННЯ НАСІННЯ ПЕЛюШКИ бІОПРЕПАРАТАМИ ВІТчИЗНЯНОгО ВИРОбНИЦТВА |
| title_fullStr | СТИМуЛюВАННЯ ПРОРОСТАННЯ НАСІННЯ ПЕЛюШКИ бІОПРЕПАРАТАМИ ВІТчИЗНЯНОгО ВИРОбНИЦТВА |
| title_full_unstemmed | СТИМуЛюВАННЯ ПРОРОСТАННЯ НАСІННЯ ПЕЛюШКИ бІОПРЕПАРАТАМИ ВІТчИЗНЯНОгО ВИРОбНИЦТВА |
| title_short | СТИМуЛюВАННЯ ПРОРОСТАННЯ НАСІННЯ ПЕЛюШКИ бІОПРЕПАРАТАМИ ВІТчИЗНЯНОгО ВИРОбНИЦТВА |
| title_sort | стимулювання проростання насіння пелюшки біопрепаратами вітчизняного виробництва |
| topic | Ризогумін Азотофіт пелюшка енергія проростання схожість насіння |
| topic_facet | rhizohumin azotofit germination rate germination Ризогумін Азотофіт пелюшка енергія проростання схожість насіння |
| url | https://smic.in.ua/index.php/journal/article/view/197 |
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