ОСОБЛИВОСТІ ПРОРОСТАННЯ НАСІННЯ ТОМАТІВ ЗА ДІЇ МІКРОБНОГО ПРЕПАРАТУ БАКТОПАСЛЬОНУ
Positive influence of Bactopaslon on the tomato seeds germination rates was observed. It was shown that application of microbial preparation improves seeds germination 10 % to 25 %, germination rates – from 19% to 36%, germination evenness – from 2 % to 6 %, speed of seeds germination – from 29 % to...
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| Дата: | 2013 |
|---|---|
| Автор: | |
| Формат: | Стаття |
| Мова: | Англійська Українська |
| Опубліковано: |
Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine
2013
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| Онлайн доступ: | https://smic.in.ua/index.php/journal/article/view/199 |
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| Назва журналу: | Agriciltural microbiology |
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Репозитарії
Agriciltural microbiology| _version_ | 1871465719486480384 |
|---|---|
| author | Євтушенко , Т.А. |
| author_facet | Євтушенко , Т.А. |
| author_institution_txt_mv | [
{
"author": "Т.А. Євтушенко ",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
}
] |
| author_sort | Євтушенко , Т.А. |
| baseUrl_str | https://smic.in.ua/index.php/journal/oai |
| collection | OJS |
| datestamp_date | 2026-07-22T10:10:44Z |
| description | Positive influence of Bactopaslon on the tomato seeds germination rates was observed. It was shown that application of microbial preparation improves seeds germination 10 % to 25 %, germination rates – from 19% to 36%, germination evenness – from 2 % to 6 %, speed of seeds germination – from 29 % to 64 % and weight of seedlings – from 45 % to 113 %. |
| doi_str_mv | 10.35868/1997-3004.17.58-66 |
| first_indexed | 2025-07-17T12:24:26Z |
| format | Article |
| fulltext |
CHARACTERISTICS OF TOMATO SEEDS GERMINATION UNDER THE
INFLUENCE OF MICROBIAL PREPARATIONS BACTOPASLON
Yevtushenko T. A.
Institute of Agricultural Microbiology and Agroindustrial Manufacture, NAAS,
Chernihiv
97, Shevchenko str., Chernihiv, 14027
E-mail: tatianazherebor@rambler.ru
Positive influence of Bactopaslon on the tomato seeds germination rates was
observed. It was shown that application of microbial preparation improves seeds
germination 10 % to 25 %, germination rates – from 19% to 36%, germination
evenness – from 2 % to 6 %, speed of seeds germination – from 29 % to 64 % and
weight of seedlings – from 45 % to 113 %.
Keywords: Bactopaslon, tomatoes, seeds germination, germination rates, seeds
germination evenness, speed of seeds germination, weight of seedlings.
Germination of seeds is the most vulnerable stage of plants development
characterized with the minimal plants resistance to various adverse factors [1]. Thus,
it is relevant to study the effect of microbial preparations on the germination of
tomato seeds. In order to increase the efficiency of microbial preparations action, it is
perspective to the use plant lectins in the technologies of their production. However,
due to the very low output of tomato lectin, its use in the manufacture of microbial
preparations increases a lot their cost. By its physic-chemical and biological
properties and interaction with carbohydrates and glucoconjugates tomato lectin is
similar to potato lectin, which is much more accessible and cheaper. In addition, the
structure of tomato lectin is similar to the lectins structure of Solanaceae family
plants [2]. Thus, we developed a microbial preparation Bactopaslon based on
consortium of Azotobacter vinelandii and Azotobacter chroococcum strains,
cultivated with potato lectin, which effectiveness was proven in our previous work on
potato cultivation technology [3].
Taking that into the account, the purpose of given research was to study the
effect Bactopaslon on tomatoes seed germination of different maturity groups.
Materials and methods. Microbial preparation Bactopaslon, based on the
consortium of bacterial strains Azotobacter chroococcum and Azotobacter vinelandii
[4], cultivated with potato lectin was used. Influence of Bactopaslon on seed
germination of was investigated on tomato plants of Flora, Rozheviy gigant and
Slavskii rozheviy varieties in laboratory conditions. As positive control, we used the
biological preparation Azotobakteryn recommended for application in cultivation
techniques of vegetable crops [5].
The influence of microbial preparations on the germination of tomatoes,
energy, seedling vigor, speed of germination and seedling mass was determined by
conventional methods [6, 7]. Seeds of tomatoes were grown in Petri dishes on filter
paper previously moistened with sterile tap water, treated with microbial
preparations, diluted with water at 1:100 ratio. The following experimental scheme
was used: 1) control (seeds treated with water); 2) seeds treatment with
Azotobakteryn; 3) seeds treatment Bactopaslon. Repetition of experiments – three
fold. Seeds were grown in incubator at 25 °C. The evaluation of studied parameters
of seeds germination was performed on a daily basis.
The data obtained was used for variation statistics analysis using the software
package «Excel – 2010» and «Statistica 6.0», difference probability was determined
using Student t-test [8].
Results and discussion. Germination and germinating rate are a key indicators
of seeds quality, affecting the productivity of crops [1, 6]. Seeds germination – its
ability to form well -developed seedlings is differentiate on laboratory (germination
in the laboratory conditions ensuring proper germination of most of the analyzed
seeds) and field (in field conditions).
Treatment of tomato seeds with Bactopaslon had ensured the highest
germination rates in laboratory tests. The germination rates in this variant had
exceeded the control values in tomato varieties Flora on 17%, Rozheviy gigant – on
25% Slavskii rozheviy – on 10% (Fig. 1). At this, the increase of studied parameters
in variants with seeds treatment with Azotobakteryn had exceeded the control values
on 10%, 18 % and 7 %, respectively.
FIGURE 1. Influence of microbial preparations on germination of tomato seeds
Along with laboratory germination, the seeds germinating energy was
determined – sprouting ability for rapid germination and seedling vigor that
characterizes seeds viability [7]. High germinating energy foresees that the seedlings
are strong and resistant to adverse environmental conditions during sowing and
emerging [1]. Numerous studies have shown that seeds with higher germinating
energy have better seedling vigor and evenness, while seeds with the same
germination, but with low germinating energy values. Field germination of seeds with
low germination energy is decreasing more intensively: field germination period is
increasing, which in turn increases the risk of plant damage with fungal diseases and
pests [9].
0
10
20
30
40
50
60
70
80
90
100
Control Azotobakteryn Bactopaslon
Se
ed
s g
er
m
in
at
io
n,
%
Variants
Flora Rozheviy gigant Slavskii rozheviy
It was established that the energy of tomato seeds germination had significantly
increased in the variants with microbial preparation use. Thus, Azotobakteryn had
increased the germination energy of tomato seeds of Flora variety on 10 % in
compared to control, on 16% - in Rozheviy gigant variety, and on 20% in Slavskii
rozheviy variety (Fig. 2). Bactopaslon appear to be more effective. In variants with
seeds treatment with Bactopaslon the germination energy of tomato seeds of studied
varieties had increased on 19%, 22% and 36%, correspondingly, if compared to
control.
FIGURE 2. Influence of microbial preparation on germination energy of tomato
seeds
The positive impact of studied microbial preparations on germination and
germinating energy of tomato seeds can be explained by the ability of bio-agents
(bacteria of the genus Azotobacter) to synthesis of plant hormones, since hormones
and hormone-like substances are interaction mediators between plants and
microorganisms [10]. Free living diazotrophs of Azotobacter genus are characterized
by their ability to accumulate high amount of hormones of auxin and cytokinin nature
in the culture medium (up to 200 mkg/dm3) [11, 12].
Phytohormonal compounds synthesized by Azotobacter can play an important
role in the exchange of metabolic products between plants and bacteria, that
positively affects the ryzoplan colonization with soil microflora and, respectively,
plants growth processes in (due to changes in the plant hormonal pool, and the ratio
of auxin and cytokinin in tissues) [11].
Germinating energy and laboratory germination are often correspond in
quantitative analysis, so in order to have more differentiated seeds characteristics the
germination rate, seedlings vigor and growth strength are used as additional
parameters. Germination rate shows the average duration of one seed germination (in
days), while the seedlings vigor – the average number of seeds that sprouted per day.
0
10
20
30
40
50
60
70
80
90
100
Control Azotobakteryn Bactopaslon
Se
ed
s g
er
m
in
at
in
g
en
er
gy
, %
Variants
Flora Rozheviy gigant Slavskii rozheviy
As it was shown bacterial preparations also had a positive effect on seedlings vigor of
tomato seeds. Thus, the most sensitive to the action of biological products was tomato
seeds of Rozheviy gigant variety. Treatment with Azotobakteryn had promoted
increase of seedlings vigor on 4%, if compared to control, while application of
Bactopaslon – on 6 % (Fig. 3).
FIGURE 3. Influence of microbial preparations on the seedlings vigor of tomato
seeds
The positive effect of microbial preparations on seedlings vigor was also
observed experiments with other tomato varieties, but those values were within the
error of experiment range.
The rate of seed germination had also increased under the influence of microbial
preparations. Thus, treatment of tomato seeds with Azotobakteryn had increased the
rate of seeds germination in comparison to control in Flora variety on 19 %, and on
27% in varieties Rozheviy gigant and Slavskii rozheviy (Fig. 4). Application of
Bactopaslon had stimulated the rate of seeds germination on 29%, 43% and 64 %,
respectively. It should be noted that treatment of seeds with Bactopaslon had boosted
the germination rate if compared to Azotobakteryn on 8% for Flora variety, on 12%
for variety Rozheviy gigant and on 29 % for variety Slavskii rozheviy.
The ability to form normal seed sprouts in field conditions promote emerging
and develop into productive plants, or the growth strength of seeds, is the another
additional parameter used for determination of seeds quality in the laboratory. Seeds
growth strength has a high correlation with field germination and is characterized by
two parameters: the number and weight of shoots that appear over a certain period of
seeds germination.
0
5
10
15
20
25
Control Azotobakteryn Bactopaslon
Se
ed
lin
gs
v
ig
or
, %
Variants
Flora Rozheviy gigant Slavskii rozheviy
FIGURE 4. Influence of microbial preparations on the germination rate of tomato
seeds
Our results had shown that microbial preparations have a positive effect on the
formation of tomato seedlings due to the significant increase of their green weight in
comparison with the control variant. It should be noted, that treatment of seeds of all
varieties with Bactopaslon had significantly increased those values. Figure 5 shows
that tomato seedlings of Flora variety had formed higher biomass in the variants with
Bactopaslon treatment which exceeded the control values on 45 %, while
Azotobakteryn aplication had promoted biomass accumulatioon on 30 %.
A similar dependence was observed also for the tomato of Rozheviy gigant variety:
biomass of seedlings in the variants with Bactopaslon was 62 % higher than in
controls and 24 % higher if compared with seedlings biomass in the variants with
Azotobakteryn.
It should be noted that the most significant biological effect on seedlings
formation was observed in tomatoes of Slavskii rozheviy variety. The studied
parameter in the variants with Bactopaslon application was two times higher if
compared to the control and exceeded Azotobakteryn effect on 47%.
The data presented indicate that Bactopaslon had influenced the germination of
tomato seeds in a greater extent than Azotobakteryn, which is quite obvious, since
add-on of the potato lectin had stimulated the synthesis of Azotobacter substances of
auxin and cytokinin nature [13].
Thus, it was established that microbial preparation Bactopaslon had increased
germination of tomato seeds, its germinating energy, seedlings vigor, germination
rates and seedling weight, indicating the possibility of implementation of this
microbial preparation into the growing technology of tomato plants.
0
5
10
15
20
25
30
35
Control Azotobakteryn Bactopaslon
Se
ed
s g
er
m
in
at
io
n
ra
te
, d
ay
s
Variants
Flora Rozheviy gigant Slavskii rozheviy
FIGURE 5. Influence of microbial preparations on the biomass accumulation of
tomato seedlings
0
10
20
30
40
50
Control Azotobakteryn Bactopaslon
Se
ed
lin
gs
m
as
s,
m
g
Variants
Flora Rozheviy gigant Slavskii rozheviy
|
| id | oai:ojs2.smic.in.ua:article-199 |
| institution | Agriciltural microbiology |
| keywords_txt_mv | keywords |
| language | English Ukrainian |
| last_indexed | 2026-07-23T01:06:30Z |
| publishDate | 2013 |
| publisher | Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine |
| record_format | ojs |
| resource_txt_mv | smicinua/f5/77589cdc9f08606fb8a295d4c16fd9f5.pdf |
| spelling | oai:ojs2.smic.in.ua:article-1992026-07-22T10:10:44Z CHARACTERISTICS OF TOMATO SEEDS GERMINATION UNDER THE INFLUENCE OF MICROBIAL PREPARATIONS BACTOPASLON ОСОБЛИВОСТІ ПРОРОСТАННЯ НАСІННЯ ТОМАТІВ ЗА ДІЇ МІКРОБНОГО ПРЕПАРАТУ БАКТОПАСЛЬОНУ Євтушенко , Т.А. Bactopaslon, tomatoes, seeds germination, germination rates, seeds germination evenness, speed of seeds germination, weight of seedlings Бактопасльон, томати, схожість насіння, дружність проростання насіння, енергія проростання насіння, швидкість проростання насіння, маса проростків Positive influence of Bactopaslon on the tomato seeds germination rates was observed. It was shown that application of microbial preparation improves seeds germination 10 % to 25 %, germination rates – from 19% to 36%, germination evenness – from 2 % to 6 %, speed of seeds germination – from 29 % to 64 % and weight of seedlings – from 45 % to 113 %. Встановлено позитивний вплив мікробного препарату Бактопасльону на проростання насіння томатів. За дії біопрепарату схожість насіння підвищувалася від 10 % до 25 %, енергія проростання – від 19 % до 36 %, дружність проростання – від 2 % до 6 %, швидкість проростання – від 29 % до 64 %. При цьому маса проростків збільшувалася на 45–113 %. Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine 2013-10-01 Article Article Рецензована Стаття application/pdf application/pdf https://smic.in.ua/index.php/journal/article/view/199 10.35868/1997-3004.17.58-66 Agricultural microbiology; Vol. 17 (2013): Agriciltural microbiology; 58-66 Сільськогосподарська мікробіологія; Том 17 (2013): Сільськогосподарська мікробіологія; 58-66 1997-3004 10.35868/1997-3004.17 en uk https://smic.in.ua/index.php/journal/article/view/199/279 https://smic.in.ua/index.php/journal/article/view/199/280 Авторське право (c) 2013 T. A. Yevtushenko https://creativecommons.org/licenses/by/4.0 |
| spellingShingle | Бактопасльон томати схожість насіння дружність проростання насіння енергія проростання насіння швидкість проростання насіння маса проростків Євтушенко , Т.А. ОСОБЛИВОСТІ ПРОРОСТАННЯ НАСІННЯ ТОМАТІВ ЗА ДІЇ МІКРОБНОГО ПРЕПАРАТУ БАКТОПАСЛЬОНУ |
| title | ОСОБЛИВОСТІ ПРОРОСТАННЯ НАСІННЯ ТОМАТІВ ЗА ДІЇ МІКРОБНОГО ПРЕПАРАТУ БАКТОПАСЛЬОНУ |
| title_alt | CHARACTERISTICS OF TOMATO SEEDS GERMINATION UNDER THE INFLUENCE OF MICROBIAL PREPARATIONS BACTOPASLON |
| title_full | ОСОБЛИВОСТІ ПРОРОСТАННЯ НАСІННЯ ТОМАТІВ ЗА ДІЇ МІКРОБНОГО ПРЕПАРАТУ БАКТОПАСЛЬОНУ |
| title_fullStr | ОСОБЛИВОСТІ ПРОРОСТАННЯ НАСІННЯ ТОМАТІВ ЗА ДІЇ МІКРОБНОГО ПРЕПАРАТУ БАКТОПАСЛЬОНУ |
| title_full_unstemmed | ОСОБЛИВОСТІ ПРОРОСТАННЯ НАСІННЯ ТОМАТІВ ЗА ДІЇ МІКРОБНОГО ПРЕПАРАТУ БАКТОПАСЛЬОНУ |
| title_short | ОСОБЛИВОСТІ ПРОРОСТАННЯ НАСІННЯ ТОМАТІВ ЗА ДІЇ МІКРОБНОГО ПРЕПАРАТУ БАКТОПАСЛЬОНУ |
| title_sort | особливості проростання насіння томатів за дії мікробного препарату бактопасльону |
| topic | Бактопасльон томати схожість насіння дружність проростання насіння енергія проростання насіння швидкість проростання насіння маса проростків |
| topic_facet | Bactopaslon tomatoes seeds germination germination rates seeds germination evenness speed of seeds germination weight of seedlings Бактопасльон томати схожість насіння дружність проростання насіння енергія проростання насіння швидкість проростання насіння маса проростків |
| url | https://smic.in.ua/index.php/journal/article/view/199 |
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