ЧИСЕЛЬНІСТЬ ФОСФАТМОБІЛІЗУВАЛЬНИХ БАКТЕРІЙ У КОРЕНЕВІЙ ЗОНІ РОСЛИН ПШЕНИЦІ ЗА ДІЇ ПОЛІМІКСОБАКТЕРИНУ ТА МІНЕРАЛЬНИХ ДОБРИВ
The paper presents the results of studies of the impact of microbial preparation Polymyxobacteryn and different doses of mineral fertilizers on the development of phosphorous mobilizing bacteria in rhizosphere soil of winter wheat plants (Sonechko variety). It was shown that quantity phosphorous mob...
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| Дата: | 2013 |
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Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine
2013
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Agriciltural microbiology| _version_ | 1871465723682881536 |
|---|---|
| author | Ковпак , П.В. Токмакова , Л.М. Ларченко , І.В. Трепач , А.О. |
| author_facet | Ковпак , П.В. Токмакова , Л.М. Ларченко , І.В. Трепач , А.О. |
| author_institution_txt_mv | [
{
"author": "П.В. Ковпак ",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "Л.М. Токмакова ",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "І.В. Ларченко ",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "А.О. Трепач ",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
}
] |
| author_sort | Ковпак , П.В. |
| baseUrl_str | https://smic.in.ua/index.php/journal/oai |
| collection | OJS |
| datestamp_date | 2026-07-22T10:10:44Z |
| description | The paper presents the results of studies of the impact of microbial preparation Polymyxobacteryn and different doses of mineral fertilizers on the development of phosphorous mobilizing bacteria in rhizosphere soil of winter wheat plants (Sonechko variety). It was shown that quantity phosphorous mobilizing bacteria increases under the influence of bacterization and fertilizers in doses not exceeding N60P60K60. |
| doi_str_mv | 10.35868/1997-3004.17.101-110 |
| first_indexed | 2025-07-17T12:24:27Z |
| format | Article |
| fulltext |
QUANTITY OF PHOSPHOROUS MOBILIZING BACTERIA IN THE ROOT
ZONE OF WHEAT UNDER THE INFLUENCE POLYMYXOBACTERYN AND
FERTILIZERS
Kovpak P. V., Tokmakova L. M., Larchenko I. V., Trepach A. O.
Institute of Agricultural Microbiology and Agricultural Production NAAS
97, Shevchenko Str., Chernihiv, 14027, Ukraine
E-mail: tokmakova_ln@mail.ru
The paper presents the results of studies of the impact of microbial preparation
Polymyxobacteryn and different doses of mineral fertilizers on the development of
phosphorous mobilizing bacteria in rhizosphere soil of winter wheat plants (Sonechko
variety). It was shown that quantity phosphorous mobilizing bacteria increases under the
influence of bacterization and fertilizers in doses not exceeding N60P60K60.
Keywords: mineral fertilizers, Polymyxobacteryn, winter wheat, phosphorous
mobilizing bacteria.
Phosphate nutrition plays an important role in yield formation of agricultural crops,
including wheat [1]. Due to the inaccessibility of certain forms of phosphorus for plants
their growth and productivity is often limited by the presence of water-soluble phosphorus
compounds [2]. Therefore, the problem of increasing the availability of phosphorus for
cultured crops from fertilizers and soil requires attention of scientists. Utilization of
techniques and methods capable to enhance activity of soil microorganisms, which play
major role in the transformation of phosphorus compounds of soil and fertilizers, is one of
the solutions. Due to the high reproduction rates bacteria is involved in the biological
cycle of many elements, including phosphorus [3, 4].
Application of microbial preparations basing on microorganisms capable of
metabolic conversion of soluble soil phosphate in soluble forms is one of the effective
measures to enhance the development of phosphorous mobilizing bacteria in soils of
agrocenosis [5-7]. In addition to improvement of phosphorus nutrition of bacterized plants,
microbial preparations stimulate growth of other members of certain ecological and
trophic groups of microorganisms in rhizosphere soil that indirectly indicates on the
positive metabolic changes in plants [8, 9]. However, the effectiveness of the introduction
of active strain – biological agent of preparation and its influence on the microbial
associations in root areas of bacterized plants depends on fertilizers background. In this
context, the aim of our study was to investigate the quantity of phosphorous mobilizing
bacteria in the rhizosphere of winter wheat plants treated with Polymyxobacteryn and
different doses of mineral fertilizers.
Materials and methods. The study was conducted during the 2011–2012 years on
winter wheat (Sonechko variety) in stationary field experiment in the Institute of
Agricultural Microbiology and Agricultural Production NAAS on meadow-chernozem
soils containing 2.12% humus, 95.2 mg / kg of easily hydrolyzed nitrogen, 226 mg / kg of
phosphorus, 108 mg / kg of exchangeable potassium, pHsalt – 5.30. The following variants
were included into the experiment scheme: 1 – without fertilizer (control), 2 – N30P30K30
(N20 in autumn + N10 at tillering stage), 3 – N60P60K60 (N20 in autumn + N40 at tillering
stage), 4 – N90P90K90 (N20 in autumn + N40 at tillering stage + N30 at booting stage), 5 – 8 –
same variants combined with bacterization. Research plots area was 86.4 sq.m (7.2 m x
12.0 m), repetition of the experiment – four-fold. Winter wheat farming techniques –
common to the Polissya region. Pre-sowing seeds treatment was performed in accordance
to the SOU 01.11-37-782:2008 [10].
Samples of rhizosphere soil were taken for microbiological analysis during
following phases of wheat plants development: booting, flowering and milky ripeness.
Reckoning of bacteria number capable to dissolve mineral and organic phosphorus
compounds was performed on agar nutrient medium of Muromtsev with the addition of
tricalcium phosphate or calcium phosphoglycerol [11]. Field experiments and
mathematical analysis of results were performed according to the conventional methods
[12].
Results and discussion. The results obtained in 2011 have shown that the growth of
microorganisms that dissolve mineral forms of phosphate in the rhizosphere soil of wheat
plants depends on fertilizers background. Analysis of dependence found have shown its
parabolic nature. Application of low fertilizers doses have stimulated the growth of studied
microorganisms. Use of fertilizer doses over N60P60K60 have reduced the number
phosphorous mobilizing bacteria. On the contrary, pre-sowing seeds bacterization with
Polymyxobacteryn have increased population of wheat plants rhizosphere soil bacteria.
Most likely, the quantity of bacteria have increased due to the development of the
introduced bacterial strain and effect of microbial preparation on plants growth,
intensification of photosynthesis and provision of root zone microbial communities with
plant assimilates. Under the bacterization conditions the development of bacteria was also
of parabolic nature and have clearly depended on fertilizers background (Fig. 1). Optimal
for the growth of the microorganisms of the studied group were N30P30K30 and N60P60K60
doses of fertilizer. Excess levels of mineral fertilizers in the experiment have decreased the
number of bacteria that dissolve mineral phosphates as comparing to the above mentioned
fertilizers background.
Data received in 2012 have generally confirmed the results of the fertilizers
backgrounds influence separately and in combination with pre-sowing seeds treatment
with microbial preparation on formation of microorganisms associations of studied groups
(Fig. 2).
Thus, these results obtained indicate that the optimum conditions for growth of
microorganisms that dissolve mineral forms of phosphate in the root zone of plants winter
wheat is a fertilizer application in doses not exceeding N60P60K60. Increased doses of
fertilizer reduces the number of bacteria that dissolve mineral phosphates.
FIGURE 1. Effect of bacterization and fertilizers on the quantity of bacteria that dissolve mineral forms of phosphate in the
rhizosphere soil of winter wheat plants (Sonechko variety, meadow-chernozem soils, 2011).
Booting stage Flowering stage Milky ripeness stage
FIGURE 2. Effect of bacterization and fertilizers on the quantity of bacteria that dissolve mineral forms of phosphate in
the rhizosphere soil of winter wheat plants (Sonechko variety, meadow-chernozem soils, 2012).
Booting stage Flowering stage Milky ripeness stage
Reckoning results of bacteria which hydrolyze organic forms of phosphate, have
showed some differences from the above described group of microorganisms under the
influence of fertilizers and Polymyxobacteryn. Thus, the data obtained in 2011 have
shown that the optimal conditions for the development of these bacteria in the rhizosphere
of wheat plants during the booting stage was the average dose of fertilizer (N60P60K60),
while bacterization on this background has also shown the biggest values by this
parameter – 12.0 million / g soil. A similar situation was observed during the flowering
stage – the number of bacteria in plants root zone on N60P60K background is 13.9 million/g
soil, while treatment with Polymyxobacteryn have increased this number to 21.7 million/g
soil. During milk ripeness stage, almost all variants with Polymyxobacteryn bacterization
have decreased number of this group of microorganisms, which is probably due to the
impoverishment of organic phosphorus in the root zone of winter wheat plants (Fig. 3).
The results obtained in 2012 have shown that the quantity of bacteria that hydrolyze
organic forms of phosphate has increased at application of microbial preparation
Polymyxobacteryn on all fertilizer backgrounds. The best result was noticed at the
combined use of Polymyxobacteryn and fertilizer in dose of N60P60K60. During booting
stage the number of bacteria on N60P60K60 background was 12.5 m / g soil, while in
variants with Polymyxobacteryn – 15.0 million/g soil. At next stage the number of
microorganisms has increased from 11.2 million/g soil (on N60P60K60 background) to 22.3
million/g soil (microbial preparation) (Fig. 4).
Thus, the use of fertilizers in doses that not exceed N60P60K60 in cultivation of winter
wheat plants has a positive effect on the quantity of bacteria that hydrolyze organic forms
of phosphate. It was established that Polymyxobacteryn application in combination with
mineral fertilizers in doses N60P60K60 created the best conditions for the development of
phosphorous mobilizing microorganisms.
Therefore, it was shown that intermittent dosing of fertilizer in low doses creates the
best conditions formation of phosphorous mobilizing microorganisms in the rhizosphere of
winter wheat plants. Application of microbial preparation Polymyxobacteryn has a
significantly increased the number of bacteria that dissolve both mineral and organic forms
of phosphate in the rhizosphere of winter wheat plants, especially on the N60P60K60
background.
FIGURE 3. Effect of bacterization and fertilizers on the quantity of bacteria that hydrolyze organic forms of phosphate in the
rhizosphere soil of winter wheat plants (Sonechko variety, meadow-chernozem soils, 2011).
Booting stage Flowering stage Milky ripeness stage
FIGURE 4. Effect of bacterization and fertilizers on the quantity of bacteria that hydrolyze organic forms of phosphate in the
rhizosphere soil of winter wheat plants (Sonechko variety, meadow-chernozem soils, 2012).
Booting stage Flowering stage Milky ripeness stage
|
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| institution | Agriciltural microbiology |
| keywords_txt_mv | keywords |
| language | English Ukrainian |
| last_indexed | 2026-07-23T01:06:34Z |
| publishDate | 2013 |
| publisher | Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine |
| record_format | ojs |
| resource_txt_mv | smicinua/61/8a48c1ece30420f251156c5c0b7f0661.pdf |
| spelling | oai:ojs2.smic.in.ua:article-2052026-07-22T10:10:44Z QUANTITY OF PHOSPHOROUS MOBILIZING BACTERIA IN THE ROOT ZONE OF WHEAT UNDER THE INFLUENCE POLYMYXOBACTERYN AND FERTILIZERS ЧИСЕЛЬНІСТЬ ФОСФАТМОБІЛІЗУВАЛЬНИХ БАКТЕРІЙ У КОРЕНЕВІЙ ЗОНІ РОСЛИН ПШЕНИЦІ ЗА ДІЇ ПОЛІМІКСОБАКТЕРИНУ ТА МІНЕРАЛЬНИХ ДОБРИВ Ковпак , П.В. Токмакова , Л.М. Ларченко , І.В. Трепач , А.О. mineral fertilizers, Polymyxobacteryn, winter wheat, phosphorous mobilizing bacteria фосфатмобілізувальні бактерії, пшениця озима, мінеральні добрива, Поліміксобактерин The paper presents the results of studies of the impact of microbial preparation Polymyxobacteryn and different doses of mineral fertilizers on the development of phosphorous mobilizing bacteria in rhizosphere soil of winter wheat plants (Sonechko variety). It was shown that quantity phosphorous mobilizing bacteria increases under the influence of bacterization and fertilizers in doses not exceeding N60P60K60. Представлено результати досліджень впливу мікробного препарату Поліміксобактерину та різних доз мінеральних добрив на розвиток фосфатмобілізувальних бактерій у ризосферному грунті рослин пшениці озимої сорту Сонечко. Чисельність фосфатмобілізаторів зростає за дії бактеризації та добрив у дозах, що не перевищують NN60P60K60. Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine 2013-10-01 Article Article Рецензована Стаття application/pdf application/pdf https://smic.in.ua/index.php/journal/article/view/205 10.35868/1997-3004.17.101-110 Agricultural microbiology; Vol. 17 (2013): Agriciltural microbiology; 101-110 Сільськогосподарська мікробіологія; Том 17 (2013): Сільськогосподарська мікробіологія; 101-110 1997-3004 10.35868/1997-3004.17 en uk https://smic.in.ua/index.php/journal/article/view/205/289 https://smic.in.ua/index.php/journal/article/view/205/290 Авторське право (c) 2013 P. V. Kovpak , L. M. Tokmakova , I. V. Larchenko , A. O. Trepach https://creativecommons.org/licenses/by/4.0 |
| spellingShingle | фосфатмобілізувальні бактерії пшениця озима мінеральні добрива Поліміксобактерин Ковпак , П.В. Токмакова , Л.М. Ларченко , І.В. Трепач , А.О. ЧИСЕЛЬНІСТЬ ФОСФАТМОБІЛІЗУВАЛЬНИХ БАКТЕРІЙ У КОРЕНЕВІЙ ЗОНІ РОСЛИН ПШЕНИЦІ ЗА ДІЇ ПОЛІМІКСОБАКТЕРИНУ ТА МІНЕРАЛЬНИХ ДОБРИВ |
| title | ЧИСЕЛЬНІСТЬ ФОСФАТМОБІЛІЗУВАЛЬНИХ БАКТЕРІЙ У КОРЕНЕВІЙ ЗОНІ РОСЛИН ПШЕНИЦІ ЗА ДІЇ ПОЛІМІКСОБАКТЕРИНУ ТА МІНЕРАЛЬНИХ ДОБРИВ |
| title_alt | QUANTITY OF PHOSPHOROUS MOBILIZING BACTERIA IN THE ROOT ZONE OF WHEAT UNDER THE INFLUENCE POLYMYXOBACTERYN AND FERTILIZERS |
| title_full | ЧИСЕЛЬНІСТЬ ФОСФАТМОБІЛІЗУВАЛЬНИХ БАКТЕРІЙ У КОРЕНЕВІЙ ЗОНІ РОСЛИН ПШЕНИЦІ ЗА ДІЇ ПОЛІМІКСОБАКТЕРИНУ ТА МІНЕРАЛЬНИХ ДОБРИВ |
| title_fullStr | ЧИСЕЛЬНІСТЬ ФОСФАТМОБІЛІЗУВАЛЬНИХ БАКТЕРІЙ У КОРЕНЕВІЙ ЗОНІ РОСЛИН ПШЕНИЦІ ЗА ДІЇ ПОЛІМІКСОБАКТЕРИНУ ТА МІНЕРАЛЬНИХ ДОБРИВ |
| title_full_unstemmed | ЧИСЕЛЬНІСТЬ ФОСФАТМОБІЛІЗУВАЛЬНИХ БАКТЕРІЙ У КОРЕНЕВІЙ ЗОНІ РОСЛИН ПШЕНИЦІ ЗА ДІЇ ПОЛІМІКСОБАКТЕРИНУ ТА МІНЕРАЛЬНИХ ДОБРИВ |
| title_short | ЧИСЕЛЬНІСТЬ ФОСФАТМОБІЛІЗУВАЛЬНИХ БАКТЕРІЙ У КОРЕНЕВІЙ ЗОНІ РОСЛИН ПШЕНИЦІ ЗА ДІЇ ПОЛІМІКСОБАКТЕРИНУ ТА МІНЕРАЛЬНИХ ДОБРИВ |
| title_sort | чисельність фосфатмобілізувальних бактерій у кореневій зоні рослин пшениці за дії поліміксобактерину та мінеральних добрив |
| topic | фосфатмобілізувальні бактерії пшениця озима мінеральні добрива Поліміксобактерин |
| topic_facet | mineral fertilizers Polymyxobacteryn winter wheat phosphorous mobilizing bacteria фосфатмобілізувальні бактерії пшениця озима мінеральні добрива Поліміксобактерин |
| url | https://smic.in.ua/index.php/journal/article/view/205 |
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