НОВА ІНАКТИВОВАНА ВАКЦИНА ПРОТИ ЕНЗООТИЧНОГО ЕНЦЕФАЛОМІЄЛІТУ СВИНЕЙ
A specific inactivated vaccine for prevention of enzootic encephalomyelitis (Teschen disease) of swines was created. The vaccine is produced on the basis of «Dniprovskiy-34» strain of Porcine teschovirus-1 isolated in the Ukraine and adjuvant of ASRIAP. During vaccines production the original method...
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Agriciltural microbiology| _version_ | 1871465742823587840 |
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| author | Дерев’янко , С.В. Сорока , В.І. Бова , Т.О. |
| author_facet | Дерев’янко , С.В. Сорока , В.І. Бова , Т.О. |
| author_institution_txt_mv | [
{
"author": "С.В. Дерев’янко ",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "В.І. Сорока ",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "Т.О. Бова ",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
}
] |
| author_sort | Дерев’янко , С.В. |
| baseUrl_str | https://smic.in.ua/index.php/journal/oai |
| collection | OJS |
| datestamp_date | 2026-07-22T10:10:44Z |
| description | A specific inactivated vaccine for prevention of enzootic encephalomyelitis (Teschen disease) of swines was created. The vaccine is produced on the basis of «Dniprovskiy-34» strain of Porcine teschovirus-1 isolated in the Ukraine and adjuvant of ASRIAP. During vaccines production the original method of teshoviruses inactivation was used. A single vaccine dose of 2 cubic cm promotes specific swines immunity to Teschen disease remaining for 11 months. Protective antibody levels were observed on the 7th day after vaccination. The maximum level of virus neutralizing antibodies in the blood of vaccinated animals on 60th day have reached 1:8192. Geometric mean titer of antibodies is 1:512, which protects the vaccinated piglets even at intracerebral introduction of 10 lethal doses of control epizootic highly virulent strain of Teschen disease of swines «Chernihivskiy-2372». A high level of innovative product design was confirmed by commission tests conducted in cooperation with State Scientific Control Institute of Biotechnology and Microorganism Strains and protected with three patents of Ukraine for inventions and useful models. A normative product documentation (Technical Requirements of Ukraine, instructions for production and control and product insert) was created. Performed feasibility study of the «Teshovak» vaccine allows recommending it into the production and introduction into the practices of veterinary medicine. |
| doi_str_mv | 10.35868/1997-3004.17.170-184 |
| first_indexed | 2025-07-17T12:24:30Z |
| format | Article |
| fulltext |
NEW INACTIVATED VACCINE AGAINST ENZOOTIC
ENCEPHALOMYELITIS OF SWINES
Derevianko S. V., Soroka V. I., Bowa T. O.
Institute of Agricultural Microbiology and Agricultural Production NAAS,
97, Shevchenko Str., Chernihiv, 14027, Ukraine
E-mail: biopreparat@mail.ru
A specific inactivated vaccine for prevention of enzootic encephalomyelitis
(Teschen disease) of swines was created. The vaccine is produced on the basis of
«Dniprovskiy-34» strain of Porcine teschovirus-1 isolated in the Ukraine and
adjuvant of ASRIAP. During vaccines production the original method of teshoviruses
inactivation was used. A single vaccine dose of 2 cubic cm promotes specific swines
immunity to Teschen disease remaining for 11 months. Protective antibody levels
were observed on the 7th day after vaccination. The maximum level of virus
neutralizing antibodies in the blood of vaccinated animals on 60th day have reached
1:8192. Geometric mean titer of antibodies is 1:512, which protects the vaccinated
piglets even at intracerebral introduction of 10 lethal doses of control epizootic
highly virulent strain of Teschen disease of swines «Chernihivskiy-2372».
A high level of innovative product design was confirmed by commission tests
conducted in cooperation with State Scientific Control Institute of Biotechnology and
Microorganism Strains and protected with three patents of Ukraine for inventions
and useful models. A normative product documentation (Technical Requirements of
Ukraine, instructions for production and control and product insert) was created.
Performed feasibility study of the «Teshovak» vaccine allows recommending it
into the production and introduction into the practices of veterinary medicine.
Keywords: vaccine, Teschen disease of swines.
Despite of the rapid pace of globalization in world economy, the current trends
for realization of national development strategies in advanced countries are aimed on
the reduction of domestic production dependence on import and increase of export
potential. First of all, this is performed in order to prevent crisis phenomena,
increasing number of work places, promoting new higher standards of living and
nutrition improvement. Population provision with meat and meat products has been
and remains an important problem in agricultural production. In total meat production
pork plays a significant role due to precocity, multiple pregnancy and great slaughter
weight of animals and is traditional on the Ukrainian market. Therefore, stabilization
and further development of the swine industry is necessary.
Enzootic encephalomyelitis (Teschen disease) of swines is one of the most
dangerous infectious diseases of swines and can cause significant economic losses in
swine industry. The etiological factor of this disease is Porcine teschovirus-1. The
effectiveness of the effort against the disease depends on the timely implementation
of an anti-epizootic measures, and particularly on the quality of vaccine preparations.
Ukraine is rapidly increasing the scale of application of immunological
veterinary medicinal preparations, but existing domestic and foreign means for
specific prevention of Teschen disease [1, 2] cannot satisfy pork producers for several
reasons: antigenic discrepancy of production strains isolated in the early 70s of last
century to epizootic [3], high reactogenicity of vaccines, lack of intensity of post-
vaccination immunity, multiple input, high products viscosity, etc. As a result of
epizootic monitoring the low reactivity of swines vaccinated against Teschen disease
was established, indicating a lack of immunogenicity of existing vaccines and the
need for continuous monitoring of the intensity of post-vaccination immunity in
farms and need of additional financial costs.
With this in mind, the goal of given study was to create an up-to-date domestic
inactivated vaccine against the enzootic encephalomyelitis of swines – «Teshovak».
Materials and methods. Epizootic, standard, reference and production strains
of virus of Porcine teschovirus-1 species «Dniprvskiy-32», « Dniprvskiy-33», «
Dniprvskiy-34», «Gorodnyanskiy-31», «Perechynskiy-642», «Chernihivskiy- 2372 «,
Talfan, Teschen 199 and Tirol from the collection of the Institute of Agricultural
Microbiology and Agricultural Production of UAAS together with hyperimmune
rabbit serum to these strains obtained using own modified scheme were used in the
experiments [7].
Studies were performed on passaging cell cultures of embryonic swine kidney
lines (SNEV), kidneys of newborn Syrian hamster (BHK-21), passaging cells of
piglets testicles (TAP) and kidneys (PK-15). Culture media 199, Ihla, 0.5% solution
of albumin hydrolyzate lactate and cattle serum were used for maintenance of cell
cultures.
Virus titer was determined by serial dilutions of cell cultures and calculated by
Reed L.J. and Muench H. method [8].
The antigenic relationship of virus strains were established in cross-reaction of
neutralization of virus in cell culture at 100 TCD50 of viral antigen and 10
neutralizing doses of hyperimmune blood sera [9]. Determination of antibody titer in
serum of experimental animals was carried out by neutralization reaction at 100
TCD50 of virus and serial two-time dilutions of blood serum in accordance to
developed guidelines [10].
Virus inactivation was performed in accordance to our ethyleneimine
technology [5].
In production of experimental models of vaccine adjuvants of ASRIAP
(Russian Federation) [11] and ISA 25 (France) were used. Four experimental samples
of inactivated vaccine against the Teschen disease of swines were produced: sample 1
– strain «Dniprovskiy-34» with ISA 25 adjuvant, sample 2 – strain «Dniprovskiy-34»
with adjuvant ASRIAP, sample 3 – strain «Gorodnyanskiy-31» with ISA 25 adjuvant,
sample 4 – strain «Gorodnyanskiy-31» with adjuvant ASRIAP. Adjuvants were
mixed with virus containing raw materials at 1:1 ratio, allowing to obtain an emulsion
of «water in oil» type, which is more easily packaged and introduced to animals.
The study of antigenic properties of four experimental samples of inactivated
vaccine against Teschen disease of swines was performed on piglets of 2 months old.
Piglets were inoculated in doses of 2 cubic cm. Observation period – 5 months.
Commission trials in cooperation with State Scientific Control Institute of
Biotechnology and Microorganism Strains of experimental sample of inactivated
vaccine Teschen based on the «Dniprovskiy-34' strain and adjuvant ASRIAP were
performed on animals in the vivarium of the Institute of Agricultural Microbiology
and Agricultural Production NAAS.
Harmlessness, immunogenicity and specificity of an inactivated vaccine was
determined on piglets of 3.5 months old. Animals of group I were introduced
intramuscularly with 1 dose of vaccine (2 cubic cm). Animals of group II were not
vaccinated. After 30 days the animals of groups I and II were intracerebrally infected
with virulent epizootic strains of the Teschen virus «Chernihivskiy-2372» at a dose of
0.05 cubic cm (10 LD50) with a titer of 4,5 lg TCD50 / cubic cm. The clinical
observations were conducted daily by measurement of body temperature for 50 days.
To determine the harmlessness and areactogenicity the double dose of vaccine
(4 cubic cm) was injected intramuscularly to piglets 3.5 months old. Observation
period – 14 days.
Experiments on establishing the shelf life of vaccine were performed with
rabbits. Animals of group I were injected intramuscularly with single dose (2 cubic
cm) of vaccine, that was stored for 12 months at a temperature of 2-8 C. Animals of
group II have received freshly made vaccine. The dynamics of virus neutralization
antibodies formation in the blood serum was observed.
Vaccine implementation system was tested in the PJSC «Snov» (Chernigov
region) on newborn piglets from vaccinated sows and piglets of different age groups.
Determination of vaccine the cost-effectiveness was performed using the
guidelines developed by the Institute of Agricultural Microbiology and Agricultural
Production NAAS [12].
Results and discussions. Strains of teshoviruses of swines «Dniprovskiy-32»,
«Dniprovskiy-33», «Dniprovskiy-34», «Gorodnyanskiy-31» were isolated as a result
of virological monitoring on the territory of Ukraine. Based on the analysis for
antigenic affinity and differences for experimental vaccine batches «Dniprovskiy-34»
and «Gorodnyanskiy-31» strains were selected. It was found that «Dniprovskiy-34»
strain is antigenically related to the reference strains Porcine teschovirus-1 Talfan,
Teschen 199 and Tirol, epizootic control strain «Chernihivskiy-2372» at 100% and
vaccine strain «Perechynskiy-642» of enzootic encephalomyelitis virus (Teschen
disease ) of swines by 67%. Antigenic affinity of «Gorodnyanskiy-31» strain with
epizootic, production and reference strains was within the range from 73 to 100%.
Based on selected perspective teshoviruses strains «Dniprovskiy-34» and
«Gorodnyanskiy-31» and two adjuvants four experimental samples of inactivated
vaccine against teshovirus encephalomyelitis of swines were produced and used in
the piglets study.
As the experiment results the immunogenic properties of four experimental
samples of inactivated vaccine were studied (Fig. 1). The maximum level of
antibodies in the blood of vaccinated animals (1:8192) was observed in variants with
sample #2, based on «Dniprovskiy-34» strain with the infectious activity of 8,2 lg
TCD50/cubic cm and adjuvant ASRIAP. Geometric mean titer of virus neutralizing
antibodies in response to its introduction for the entire period of the experiment was
1:588. At introduction of the sample #1 of the same strain and ISA 25 adjuvant the
largest titer was 1:512 with the average level of antibodies – 1:84. For sample #3 and
#4 based on «Gorodnyanskiy-31» strain with an infectious activity of 7,5 lg TCD50 /
cubic cm and ISA 25 adjuvant and ASRIAP the numbers were 1:52 and 1:128 and
1:512 and 1:64, respectively. The protective level of virus neutralizing antibodies
upon introduction of sample #2 was formed in 14 days, while for the other
vaccination vaccine samples – 21–30 days.
At this the postvaccination virus neutralizing antibodies have persisted in the
serum blood of immunized animals with titer 1:128-1:2048 for 6 months. Antibody
titers have remained above the protective level during the next 5 months of
observation.
Thus, the sample #2, based on «Dniprovskiy-34» strain and adjuvant ASRIAP
appear to be the most promising and was selected for further studies.
Selected experimental sample of inactivated vaccine against the Teschen
disease of swines was put into the commission trials. It was established that given
vaccine is harmless and non-reactogenic. Piglets that received double dose of vaccine
intramuscularly (4 cubic cm), within the 14-days observation were clinically healthy,
without tissue inflammation at the injection site, clinical complications or adverse
effects on body weight increase (Table 1).
FIGURE 1. Dynamics of the immune response in swines vaccinated with the
experimental samples of inactivated vaccine against the Teschen disease of swines.
Sample #1 – strain «Dniprovskiy-34» with ISA 25 adjuvant,
sample #2 – strain «Dniprovskiy-34» with ASRIAP adjuvant,
sample #3 – strain «Gorodnyanskiy-31» with ISA 25 adjuvant,
sample #4 – strain «Gorodnyanskiy-31» with ASRIAP adjuvant.
The protective level of virus neutralizing antibodies in piglets (1:64 or higher)
was observed within 7 days after vaccination. Geometric mean titer at this was 1:104.
Maximum antibody levels were observed in 21–28 days. Their geometric mean titer
was 1:1024. Individual characteristics of piglet organisms have not significantly
affect the formation of immune responses. After 28 days the level of antibodies in
animals was 1:1024 with geometric mean titer increased to 1:512.
0
1000
2000
3000
4000
5000
6000
7000
8000
9000
0 7 14 21 30 60 90 120 150 180
Se
ru
m
d
ilu
tio
ns
Days after vaccination
Sample #1 Sample #2 Sample #3 Sample #4
TABLE 1. Harmlessness and non-reactogenicity of experimental sample of
inactivated vaccine against Teschen disease of swines based on the «Dniprovskiy-34»
strain
Group of
animals
№ of
piglets
live weight, kg Increase of live
weight, kg
Daily increase,
mean, g
Before the
experi-
ment
After
the
experi-
ment
By each
animal
By
group
By each
animal
By
group
І
(experim
ental)
1 19,0 23,0 4,0
11,5
285,7
273,8 2 17,5 22,0 4,5 321,4
3 14,0 17,0 3,0 214,3
ІІ
(control)
4 13,5 17,0 3,5
10,0
250,0
238,1 5 16,5 21,0 4,5 285,7
7 12,0 14,0 2,0 142,9
The antigenic activity of given samples of inactivated vaccine on piglets was
established (Table 2).
Results of immunogenicity study of experimental sample of inactivated
vaccine against Teschen disease of swines are presented in Table 3.
TABLE 2. Antigenic activity of the experimental sample of inactivated vaccine
against Teschen disease of swines based on «Dniprovskiy-34» strain
№№ Piglets Antibody titers* per day Geometric mean
titer 0 7 14 21 28
6 4 64 256 512 1024 256
8 0 128 512 1024 1024 512
9 2 128 256 2048 1024 512
Geometric mean titer 2 104 341 1024 1024
Note. * – Antibody titers are in the reciprocal values.
TABLE 3. Immunogenic activity of the experimental sample of inactivated vaccine
against Teschen disease of swines, based on «Dniprovskiy-34» strain
Group of animals №№ animal Infection results
sick/dead (days)
І (experimental)
6
Clinically healthy 8
9
II (control) 4 11 14
7 23 26
After 30 days animals of the group I that were vaccinated intramuscularly with
the single dose of 2 cubic cm and animals of the group II (without vaccination) were
intracerebrally infected with epizootic control strain «Chernihivskiy-2372» of
Teschen disease virus. Observation results have shown that vaccinated piglets
remained clinically healthy throughout the observation period (50 days). Control
piglets on 11th and 23rd days got sick with symptoms of Teschen disease: food denial,
ohlum-like state, ataxia, tremor of body muscles, shortness of breath, increase of skin
sensitiveness, clonic and toxic convulsions, squealing, paresis and paralysis of the
limbs. Piglet #4 have died within 14 days. Piglet #7 was slaughtered after 26 days
after infection. Autopsy have revealed pathological body changes typical for Teschen
disease.
Study of vaccine application system have revealed that in blood serum of
piglets born from vaccinated sows (identification on the 15th day after piglets) the
geometric mean titer of virus neutralizing colostral antibodies were 1:208 (Table 4).
TABLE 4. Antigenic activity of the experimental sample of vaccine against Teschen
disease of swines in piglets of different ages
Piglets age
Geometric mean titers of antibodies*
Colostral antibodies,
after/days after/months
0 15 30 45 60 1 2 3 4 5 6 7
15–60 days – 208 91 39 14 – – – – – – –
30–45 days – – – – – 111 91 111 – – – –
3.5–4 months – – – – – 512 676 588 588 676 446 512
Note. * – Antibody titers are in reciprocal values.
On the 15th day, a gradual decrease of antibodies was noticed. Within the 60
days their level was 1:14. In piglets vaccinated against the Teschen disease prior the 2
months age, the immune response was lower as compared with the ones vaccinated at
the age of 3.5–4 months. Swines that were vaccinated in 3.5–4 months age the titer of
virus neutralizing antibodies after 7 months have remained at protective levels and
were 1:512.
To determine vaccine suitability after 12 months of storage at a temperature of
2–8 C rabbits were injected intramuscularly with single dose (2 cubic cm) of vaccine
against Teschen disease. Investigation results of blood serum of rabbits injected with
freshly prepared vaccine against Teschen disease and with vaccine after 12 months of
storage are depicted in Table 5. In 14 days after introduction of freshly prepared
vaccine the titers of antibodies in the blood serum of rabbits were 1: 256–1:512. After
12 months of storage vaccine have stimulated immune response at 1:128–1:256. At
21st day, the immune response of rabbits to vaccination was almost leveled. Virus
neutralizing titers of antibodies in the blood serum of rabbits 28 days after vaccine
introduction of freshly prepared and stored for 12 months vaccines were respectively
1:1024–1:2048 and 1:128–1:512.
The results obtained, have shown that after the 12 months of storage at 2–8 C
the experimental sample of inactivated vaccines against the Teschen disease was
active and utilizable.
Based on these data the normative product documentation (Technical
Requirements of Ukraine, instructions for production and control and product insert)
for vaccine was created.
TABLE 4. Antigenic activity of the experimental sample of vaccine against Teschen
disease of swines freshly made (C) and after 12 months of storage (S)
№№
rabbits
Titers of virus neutralizing antibodies* after/days Geometric mean titers
of antibodies 14 21 28
С S С S С S С S
1, 2 512 256 256 512 2048 512 630 388
3, 4 256 256 – 512 – 512 256 388
5, 6 256 128 256 128 1024 128 388 128
Note. * – Antibody titers are in reciprocal values.
Thereby, a new domestic inactivated vaccine «Teshovak» was created on the
basis of highly immunogenic antigenically dominant strain «Dniprovskiy-34»
(Porcine teschovirus-1), isolated in Ukraine and deposited in the Depository of State
Scientific Control Institute of Biotechnology and Microorganisms Strains
(registration number 486) and protected with the patent of Ukraine [4].
During vaccine manufacturing the etylenimin is used for virus inactivation
which, unlike formaldehyde, inactivates nucleic acid of the virus without damaging
its capsid. This technology provides a high level of virus inactivation in a short period
of time, while maintaining the antigenic structure of the capsid inherent to the native
virus. Given inactivation method of enzootic encephalomyelitis virus (Teschen
disease) of swines is protected by patent of Ukraine for useful model [5].
ASRIAP adjuvant was used for vaccine production [11]. It has a low viscosity
and has no problems with vaccines introduction to animals even at low temperatures.
Application of new strain, adjuvant and virus inactivation technology for
ensure high innovation level of vaccine. Commission trials of inactivated vaccines
against the Teschen disease of swines confirm its reliable consumer characteristics:
high specificity, immunogenicity, immunity tension, antigenic activity, avirulence,
lack of reactogenicity, ease of use even at low temperatures. Currently this is the only
inactivated vaccine against Teschen disease of swines, which ensures such level of
immunity tension that allows swines protection against the Teschen disease of
swines, even at 10 intracerebral introduction of lethal doses of epizootic control
strain. The vaccine provides a high level of colostral antibodies in newborn piglets
from vaccinated sows and prolonged immunity tension. Created vaccine has a long
shelf life and is protected with the Patent Ukraine for useful model [6].
Combination of the above-mentioned characteristics ensures «Teshovak»
vaccine competitiveness in relation to existing domestic and foreign analogues, that is
confirmed by expert judgments and economic evaluations.
The domestic market for veterinary preparations is about 100 million USD,
with the immunological part – about 40 % [13]. Swines population in Ukraine is
more than 7.5 million. The economic effect (prevented losses) from the vaccine
introduction on farms may reach 350 million UAH per year, if vaccinating 1 million
of livestock, which is 1000 times larger the cost of its creation. The potential sales
volume of inactivated vaccines against the Teschen disease of swines «Teshovak»
(under the same conditions) can be up to 3 million UAH per year if seting single dose
price to 3 UAH.
The introduction of new vaccine into the production should improve the
epizootic situation with Teschen disease of swines, enhance development and
application of domestic scientific and technological capacity by engaging scientific
personnel into the manufacturing process. That will significantly improve the
performance of biotechnological production and create more jobs, which, in turn,
may have a positive impact on competitiveness of the domestic biotechnological
industry, reducing Ukraine's dependence on imports of immunological preparations
and promoting export of domestic immunobiological products.
|
| id | oai:ojs2.smic.in.ua:article-212 |
| institution | Agriciltural microbiology |
| keywords_txt_mv | keywords |
| language | English Ukrainian |
| last_indexed | 2026-07-23T01:06:52Z |
| publishDate | 2013 |
| publisher | Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine |
| record_format | ojs |
| resource_txt_mv | smicinua/b8/142619914ecf770cde70f71b5405f6b8.pdf |
| spelling | oai:ojs2.smic.in.ua:article-2122026-07-22T10:10:44Z NEW INACTIVATED VACCINE AGAINST ENZOOTIC ENCEPHALOMYELITIS OF SWINES НОВА ІНАКТИВОВАНА ВАКЦИНА ПРОТИ ЕНЗООТИЧНОГО ЕНЦЕФАЛОМІЄЛІТУ СВИНЕЙ Дерев’янко , С.В. Сорока , В.І. Бова , Т.О. vaccine, Teschen disease of swines вакцина, хвороба Тешена свиней A specific inactivated vaccine for prevention of enzootic encephalomyelitis (Teschen disease) of swines was created. The vaccine is produced on the basis of «Dniprovskiy-34» strain of Porcine teschovirus-1 isolated in the Ukraine and adjuvant of ASRIAP. During vaccines production the original method of teshoviruses inactivation was used. A single vaccine dose of 2 cubic cm promotes specific swines immunity to Teschen disease remaining for 11 months. Protective antibody levels were observed on the 7th day after vaccination. The maximum level of virus neutralizing antibodies in the blood of vaccinated animals on 60th day have reached 1:8192. Geometric mean titer of antibodies is 1:512, which protects the vaccinated piglets even at intracerebral introduction of 10 lethal doses of control epizootic highly virulent strain of Teschen disease of swines «Chernihivskiy-2372». A high level of innovative product design was confirmed by commission tests conducted in cooperation with State Scientific Control Institute of Biotechnology and Microorganism Strains and protected with three patents of Ukraine for inventions and useful models. A normative product documentation (Technical Requirements of Ukraine, instructions for production and control and product insert) was created. Performed feasibility study of the «Teshovak» vaccine allows recommending it into the production and introduction into the practices of veterinary medicine. Розроблено інактивовану вакцину для специфічної профілактики ензоотичного енцефаломієліту (хвороби Тешена) свиней. Вакцина виготовляється на основі штаму «Дніпровський-34» Porcine teschovirus-1, виділеного на території україни, та ад’юванту ВНДІЗТ. у процесі виробництва вакцини використовується оригінальний спосіб інактивації тешовірусів. Одноразове введення вакцини в дозі 2 см3 сприяє формуванню специфічного імунітету у свиней до збудника хвороби Тешена, що зберігається упродовж 11 місяців. Захисний рівень антитіл спостерігається вже через 7 днів після введення вакцини. Максимальний рівень віруснейтралізуючих антитіл у крові щеплених тварин на 60 добу сягав 1:8192. Середньогеометричний титр антитіл становить 1:512, що забезпечує захист вакцинованих поросят навіть при інтрацеребральному введенні 10 летальних доз контрольного епізоотичного високо вірулентного штаму хвороби Тешена свиней «Чернігівський-2372». Високий інноваційний рівень розробки підтверджено комісійними випробуваннями, проведеними спільно з співробітниками Державного науково-контрольного інституту біотехнології і штамів мікроорганізмів, та захищено 3 патентами україни на винаходи. Розроблено нормативну документацію. Проведено техніко-економічне обґрунтування застосування вакцини «Тешовак», що дозволяє рекомендувати її до виробництва та впровадження в практику ветеринарної медицини. Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine 2013-10-01 Article Article Рецензована Стаття application/pdf application/pdf https://smic.in.ua/index.php/journal/article/view/212 10.35868/1997-3004.17.170-184 Agricultural microbiology; Vol. 17 (2013): Agriciltural microbiology; 170-184 Сільськогосподарська мікробіологія; Том 17 (2013): Сільськогосподарська мікробіологія; 170-184 1997-3004 10.35868/1997-3004.17 en uk https://smic.in.ua/index.php/journal/article/view/212/304 https://smic.in.ua/index.php/journal/article/view/212/305 Авторське право (c) 2013 S. V. Derevianko , V. I. Soroka , T. O. Bowa https://creativecommons.org/licenses/by/4.0 |
| spellingShingle | вакцина хвороба Тешена свиней Дерев’янко , С.В. Сорока , В.І. Бова , Т.О. НОВА ІНАКТИВОВАНА ВАКЦИНА ПРОТИ ЕНЗООТИЧНОГО ЕНЦЕФАЛОМІЄЛІТУ СВИНЕЙ |
| title | НОВА ІНАКТИВОВАНА ВАКЦИНА ПРОТИ ЕНЗООТИЧНОГО ЕНЦЕФАЛОМІЄЛІТУ СВИНЕЙ |
| title_alt | NEW INACTIVATED VACCINE AGAINST ENZOOTIC ENCEPHALOMYELITIS OF SWINES |
| title_full | НОВА ІНАКТИВОВАНА ВАКЦИНА ПРОТИ ЕНЗООТИЧНОГО ЕНЦЕФАЛОМІЄЛІТУ СВИНЕЙ |
| title_fullStr | НОВА ІНАКТИВОВАНА ВАКЦИНА ПРОТИ ЕНЗООТИЧНОГО ЕНЦЕФАЛОМІЄЛІТУ СВИНЕЙ |
| title_full_unstemmed | НОВА ІНАКТИВОВАНА ВАКЦИНА ПРОТИ ЕНЗООТИЧНОГО ЕНЦЕФАЛОМІЄЛІТУ СВИНЕЙ |
| title_short | НОВА ІНАКТИВОВАНА ВАКЦИНА ПРОТИ ЕНЗООТИЧНОГО ЕНЦЕФАЛОМІЄЛІТУ СВИНЕЙ |
| title_sort | нова інактивована вакцина проти ензоотичного енцефаломієліту свиней |
| topic | вакцина хвороба Тешена свиней |
| topic_facet | vaccine Teschen disease of swines вакцина хвороба Тешена свиней |
| url | https://smic.in.ua/index.php/journal/article/view/212 |
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