INFLUENCE OF BIOPREPARATION RHYZOHUMIN AND PLANT GROWTH REGULATOR BIOGLOBINE ON SYMBIOTIC NITROGEN-FIXING SYSTEM AND YIELD OF SOYBEAN
The paper presents the results of study on the influence of pre-sowing treatment of soybeanseeds with the microbial preparation Rhyzohumin and growth regulator Bioglobine, and their separate application (Rhyzohumin was used for seeds bacterization while growth regulator was appliedfoliar during diff...
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Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine
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| author | Murach, O. M. Volkohon, V. V. |
| author_facet | Murach, O. M. Volkohon, V. V. |
| author_institution_txt_mv | [
{
"author": "O. M. Murach",
"institution": null
},
{
"author": "V. V. Volkohon",
"institution": null
}
] |
| author_sort | Murach, O. M. |
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| collection | OJS |
| datestamp_date | 2026-07-22T10:10:40Z |
| description | The paper presents the results of study on the influence of pre-sowing treatment of soybeanseeds with the microbial preparation Rhyzohumin and growth regulator Bioglobine, and their separate application (Rhyzohumin was used for seeds bacterization while growth regulator was appliedfoliar during different phases of plants organogenesis) on the formation and functioning of a symbiotic nitrogen-fixing apparatus in the conditions of the north-eastern forest-steppe of Ukraine.Separate application of studied preparations has intensively stimulated the formation and activityof soybean-rhizobial symbiosis, compared to their joint application. Considering their stage-bystage application the preparations has a positive action and increase the grain yield of soybeans. |
| doi_str_mv | 10.35868/1997-3004.27.52-59 |
| first_indexed | 2025-07-17T12:23:28Z |
| format | Article |
| fulltext |
52
Сільськогосподарська мікробіологія. — 2018. — Вип. 27. — С. 52–59.
ISSN 1997-3004
UDC 581.138.1:631.8:635.655
INFLUENCE OF BIOPREPARATION RHYZOHUMIN
AND PLANT GROWTH REGULATOR BIOGLOBINE
ON SYMBIOTIC NITROGEN-FIXING SYSTEM
AND YIELD OF SOYBEAN
O. M. Murach1, V. V. Volkohon2
1Institute of Agriculture of Northern East, NAAS
Zelena Str., Sad, Sumy region, 42343, Ukraine; e-mail: agronauka@gmail.com
2Institute of Agricultural Microbiology and Agroindustrial Manufacture, NAAS
97, Shevchenka str., Chernihiv, 14027, Ukraine; e-mail: volkogon@ukr.net
The paper presents the results of study on the influence of pre-sowing treatment of soybean
seeds with the microbial preparation Rhyzohumin and growth regulator Bioglobine, and their sepa-
rate application (Rhyzohumin was used for seeds bacterization while growth regulator was applied
foliar during different phases of plants organogenesis) on the formation and functioning of a sym-
biotic nitrogen-fixing apparatus in the conditions of the north-eastern forest-steppe of Ukraine.
Separate application of studied preparations has intensively stimulated the formation and activity
of soybean-rhizobial symbiosis, compared to their joint application. Considering their stage-by-
stage application the preparations has a positive action and increase the grain yield of soybeans.
Key words: soybean, bacterization, Rhyzohumin, plant growth regulator Bioglobine, soybean-
rhizobial symbiosis, yield.
One of the important issues in modern crop
production is the development and implementa-
tion of environmentally adapted technologies
for agriculture crops. Their main peculiarity is
to help plants maximally adapt to environmental
conditions to maximize the genetic capabilities
of modern varieties and highly heterozygous
hybrids. However, the variability of weather
conditions in different years requires constant
correction of the conditions for the development
of plants through the application of particular
agrotechnical means. In addition, it is well
known that the effect of the same agrotechnical
technique in different weather conditions can
provide not only the same effect, and even have
a different direction of action. It should also be
noted that it is not always possible to withstand
all components of different cultivating technol-
ogy in agricultural production. In connection
with this, there is a need for the use of certain
agrotechnical techniques that would compensate
to the greatest extent the possible crop losses,
caused by a changes in the previous agro-
technical technique or the terms of their imple-
mentation.
Considering the ability of soybean plants to
form a symbiosis with nodular bacteria and thus
to improve nitrogen nutrition conditions, many
studies have been conducted in recent years fo-
cusing on the effectiveness of the use of bacteri-
al preparations. According to long-term data,
soybean yield under the use of microbial prepa-
rations can grow by 25–30 %, and protein con-
tent in grain by 2–6 % [1; 2].
Scientific data also claim the significant pro-
spects for soybean seeds treatments with growth
regulators, which contributes to a significant in-
crease in the crop productivity at low cost. How-
ever, the information on the possibility of com-
bining pre-sowing bacterization with the use of
growth regulators is quite controversial.
In this regard, the purpose of our work was
to study the effectiveness of the use of plant
growth regulator alone and in combination with
the bacterial preparation in the cultivation of
soybean under the conditions of the north-
eastern forest-steppe of Ukraine.
Materials and methods. The study was
conducted during 2016–2017 in the field condi-
tions in the Institute of Agriculture of the North-
© O. M. Murach, V. V. Volkohon, 2018
53
East of the NAAS on a typical, low-humus,
slightly leached, large-peal-medium-loamy cher-
nozem on loess, the arable layer of which is
characterized by the following main parameters:
humus content — 4.1 %, pHsal. — 6.3, the
amount of absorbed bases is 31 mg-Eq, content
of the mobile forms of phosphorus is 11.3 mg /
/ 100 g, exchangeable potassium is 9.2 mg /
/ 100 g, content of easily hydrolyzed nitrogen by
Cornfield — 11.2 mg / 100 g of soil.
The preparations efficacy was studied on
Siverka soybean variety.
The scheme of two-factor experiments in-
cluded 16 variants (see the tables below), re-
peated four times. Variants in the repetition
were placed systematically. The total area of the
site in the experiment was 137 m2, accounting
area — 67.2 m2. The cultivating technology of
the experiment was similar to the generally ac-
cepted in given soil-climatic zone with winter
wheat pre-crop. Tillage, seeding, crop mainte-
nance and harvesting were carried out according
to zonal recommendations. Soybean seeding
rate was 850 thousand similar seeds per 1 hec-
tare, seeded with ordinary row width 15 cm.
Soybean seeds were treated with Maxim XL
035 FS (1 L/ton) 14 days before sowing and
with microbial preparation and plant growth
regulator at the day of seeding according to the
experimental scheme following the manufactur-
er’s recommendations [3]. For pre-sowing seed
inoculation a biological preparation of complex
action Rhyzohumin (TU U 24.1-00497360-003:
2007) on the basis of Bradyrhizobium japo-
nicum M-8 was used at a rate of 2 kg/ton of
seed. In addition to pre-sowing bacterization,
seeds were treated with a solution of plant
growth regulator Bioglobine (developed by
Medbiocom Ukraine) — 1,000 mL/t of seed and
foliar — 1,000 mL/ha according to the scheme
of experiment. Biometric studies and accounting
for soybeans were conducted according to gen-
erally accepted methods [4]. The effectiveness
of legume-rhizobial symbiosis was estimated at
the flowering and pod filling stage counting the
number, weight and nitrogenase activity of the
nodules per plant. The number and weight of
nodules on the roots of plants were determined
after washing out the soil-plant monoliths [5],
activity of symbiotic nitrogen fixation was
measured using the acetylene method [6]. The
statistical data analysis of the obtained results
was carried out in accordance with the existing
methods [4] and the use of the software package
Statistica 6.0.
Results and discussion. The conducted
studies indicate that the seeds treatment with the
focus preparations improves the conditions for
the formation of nitrogen-fixing nodules on the
roots. Their number in the flowering stage in
variants with microbial preparation, alone and in
combination with the growth regulator, were
significantly higher (1.1–1.2-fold) compared to
control data in 2016 (Table 1).
2017 data show in general the low numbers
of root nodules compared to the previous year,
caused by the arid weather conditions (62 % of
the average multi-year rainfall value during the
vegetation period, HTF — 0.7). Plant growth
regulator, used for seed treatment, contributed
more to the formation of nodules. A significant
increase in the parameters was also obtained
under the use of microbial preparation. Com-
bined, the use of Rhyzohumin and growth regu-
lator Bioglobine for seed treatment has no sig-
nificant increase in the number of nodules.
The study of the nodule activity in the pod
filling phase showed the increase of the number
of nodules in the studied plants, with both mi-
crobial preparation and growth regulator con-
tributed to the increase in the parameters com-
pared with the control variant. Thus, in the
phase of pod filling, the number of nodules in-
creased 1.3–1.4-fold (2016) and 1.3–1.8-fold
(2017), depending on the experimental variants
comparing to the previous period (the flowering
phase).
Significant influence of separate use of
plant growth regulator for seed treatment on the
formation of symbiotic apparatus of soybeans
was noted in 2016. Thus the number of nodules
exceeded the absolute control parameters 1.7–
fold (102.8 versus 61.3 units per plant).
Under the 2017 conditions, the maximum
increase of the number of nodules (10.9 units/
/plant versus 19.8 units/plant in absolute con-
trol) was observed during the pod filling phase
in the experimental variants with seed bacteri-
zation.
The optimal conditions for the formation of
nodules under the influence of both studied fac-
tors were different depending upon the year of
study. Thus, under the foliar use of the growth
regulator on soybeans during the phase of bud-
ding and pod filling on the background of seed
treatment with a microbial preparation in com-
ISSN 1997-3004 Сільськогосподарська мікробіологія. — 2018. — Вип. 27.
54
Table 1. The influence of microbial preparation Rhyzohumin and growth regulator Bioglo-
bine under the different methods of their application on the formation of nitrogen-fixing nodules
Seed
processing
(factor А)
Plant processing with Bio-
globine solution by
vegetation (factor B)
Number of nodules, U/plant
2016 2017
flowering
stage
pod filling
stage
flowering
stage
pod filling
stage
Control
no treatment 62.9 61.3 11.3 19.8
budding stage 66.8 80.7 17.2 19.4
grain filling stage 64.9 85.6 16.7 21.8
budding stage + grain filling
stage 65.0 90.6 17.9 22.2
Rhyzohumin
no treatment 69.4 90.9 19.0 30.7
budding stage 78.1 98.9 17.9 28.9
grain filling stage 80.2 95.1 17.3 24.7
budding stage + grain filling
stage 86.1 106.3 19.7 25.7
Bioglobine
no treatment 63.2 102.8 23.3 26.6
budding stage 70.8 112.4 21.7 28.1
grain filling stage 65.8 113.2 21.3 25.7
budding stage + grain filling
stage 73.0 138.8 17.7 31.4
Rhyzohumin +
Bioglobine
no treatment 77.9 91.1 15.2 25.0
budding stage 89.8 101.2 15.6 22.2
grain filling stage 79.0 121.9 16.6 30.3
budding stage + grain filling
stage 97.7 123.6 17.4 24.7
LSD05 for factor А
LSD05 for factor В
5.1
6.1
13.7
11.2
5.3
1.3
6.1
5.5
bination with the growth regulator, the largest
number of nodules (123.6 units per plant) was
obtained, which was more than 2.0-fold higher
compared with the values of absolute control
and 1.4-fold higher in comparison with the simi-
lar treatment of plants in the control variant in
2017, the maximum values for this parameter
(31.4 units/plant versus 19.8 units/plant in abso-
lute control) were obtained at the background of
pre-sowing treatment of the seeds by the growth
regulator with subsequent plant-foliar treat-
ments with the same preparation in two phases
of organogenesis of plants.
An important criterion for the interaction of
plants and bacteria is the mass of nodules on the
roots of legumes. Upon the investigation of pe-
culiarities of the formation of a soybean nitro-
gen-fixing apparatus, it was established that the
nodulation process was active in all variants, in-
cluding control, where the nodules were formed
due to the presence of aboriginal rhizobia in the
soil. However, not all variants of the experiment
had a positive correlation between the number
and weight of the nodules (Table 2).
It was established that prior to the pod fill-
ing stage the weight of nodules has increased
differently. Thus, at flowering stage the roots of
single soybean plant has accumulated, on aver-
age, from 0.22 to 0.44 mg (2016) and from 0.05
to 0.15 mg (2017) of nodules, while during pod
filling stage, this figure increased 1.6–1.9-fold
(2016) and 2.3–3.2-fold depending on the ex-
perimental variant in 2017 (Table 2).
It should be noted that the studied prepara-
tions influenced the formation of modules in
different ways. In particular, the variant with
pre-sowing seeds treatment with a complex of
preparations was characterized by a greater no-
dular activity, while the nodules, due to such
treatment, were smaller and had a lower weight
compared with similar parameters of the variant
with the seeds inoculation with Rhyzohumin.
A similar feature was noted when comparing
variants of seed treatment with a growth regu-
ISSN 1997-3004 Сільськогосподарська мікробіологія. — 2018. — Вип. 27.
55
Table 2. The influence of microbial preparation Rhyzohumin and growth regulator Bioglo-
bine under the different methods of their application on the dry weight of nodules
Seed
processing
(factor А)
Plant processing with Bio-
globine solution by
vegetation (factor B)
Weight of dry nodules per one plant, mg
2016 2017
flowering
stage
pod filling
stage
flowering
stage
pod filling
stage
Control
no treatment 0.22 0.43 0.09 0.16
budding stage 0.26 0.41 0.10 0.20
grain filling stage 0.24 0.47 0.12 0.17
budding stage + grain filling
stage 0.25 0.49 0.13 0.18
Rhyzohumin
no treatment 0.33 0.50 0.13 0.28
budding stage 0.41 0.58 0.12 0.26
grain filling stage 0.38 0.59 0.13 0.35
budding stage + grain filling
stage 0.44 0.71 0.15 0.29
Bioglobine
no treatment 0.25 0.48 0.10 0.23
budding stage 0.30 0.68 0.13 0.28
grain filling stage 0.32 0.67 0.06 0.31
budding stage + grain filling
stage 0.34 0.66 0.05 0.35
Rhyzohumin +
Bioglobine
no treatment 0.29 0.40 0.08 0.22
budding stage 0.36 0.48 0.13 0.21
grain filling stage 0.29 0.50 0.10 0.30
budding stage + grain filling
stage 0.39 0.54 0.11 0.26
LSD05 for factor А
LSD05 for factor В
0.08
0.09
0.05
0.08
0.03
0.02
0.09
0.06
lator and bacterization.
The most efficient symbiotic system was
formed by plants, inoculated with B. japoni-
cum — the weight of nodules during flowering
and pod filling stages was 1.2- and 1.5-fold
higher, respectively, than control parameter
(2016) (see Tables 1 and 2). The results of 2017
studies showed the same pattern. That is, for the
use of bacterial inoculum, the weight of nodules
was also the highest and increased 1.4- and 1.8-
fold relatively to the stage of development,
compared with the control variant.
Biological nitrogen fixation is an effective
ecological, energy-saving mean to increase ni-
trogen reserves in soils [9]. In this regard, it is
important to have an understanding of the inten-
sity of nitrogen fixation process and ways to
enhance it.
In our experiments the nitrogen-fixing ac-
tivity of the studied symbiotic systems varied
over the years (Table 3).
Plants in the control variant formed sym-
biotic system due to aboriginal rhizobia present
in the soil, however their nitrogen-fixing activi-
ty was low.
All investigated preparations enhanced ni-
trogenase activity compared to the control vari-
ants. However, during the flowering and pod
filling stages soybean ontogenesis, the growth
of nitrogen-fixation activity was observed main-
ly due to the use of Rhyzohumin. Pre-sowing
seed treatment by the growth regulator signifi-
cantly influenced this parameter only in 2017 —
with the transition of plants to the pod filling
stage.
It should be noted that the combined use of
Rhyzohumin and Bioglobine for seeds treatment
did not stimulated the nitrogen fixation activity,
which was lower compared to the separate use
of studied preparations. This feature was ob-
served during all studied stages in both years.
It should be noted that in 2016, the level of
nitrogenase activity of the nodules reached the
maximum during the flowering stage, decreas-
ISSN 1997-3004 Сільськогосподарська мікробіологія. — 2018. — Вип. 27.
56
Table 3. The influence of microbial preparation Rhyzohumin and growth regulator Bioglo-
bine under the different methods of their application on the nitrogenase activity of soybean root
nodules
Seed
processing
(factor А)
Plant processing with Bio-
globine solution by
vegetation (factor B)
Nitrogenase activity, nmol С2Н4 / plant per hour
2016 2017
flowering
stage
pod filling
stage
flowering
stage
pod filling
stage
Control
no treatment 423 142 225 210
budding stage 496 371 323 254
grain filling stage 442 398 247 218
budding stage + grain filling
stage 490 402 306 159
Rhyzohumin
no treatment 822 543 440 467
budding stage 1,010 658 460 689
grain filling stage 882 812 420 689
budding stage + grain filling
stage 970 829 555 963
Bioglobine
no treatment 677 413 367 579
budding stage 785 438 420 694
grain filling stage 687 460 345 631
budding stage + grain filling
stage 812 552 755 858
Rhyzohumin +
Bioglobine
no treatment 514 313 259 342
budding stage 682 339 345 362
grain filling stage 491 401 315 464
budding stage + grain filling
stage 639 592 328 444
LSD05 for factor А
LSD05 for factor В
118
87
266
255
52
88
169
174
ing with the onset of pod filling stage. However,
in the variant, where the microbial preparation
was used for seed treatment, nitrogen-fixing ac-
tivity was the highest and at the same time more
than 3.8-fold higher compared with the absolute
control parameter. The effects of both investi-
gated factors on nitrogen-fixing activity were
achieved under the seeds treatment with Rhyzo-
humin, followed by foliar application of Bioglo-
bine during budding and pod filling stages (this
parameter exceeded the value in the control var-
iant with a similar treatment of plants by the
growth regulator 2.1-fold.
The exploration of the dynamics of symbi-
otic nitrogen fixation in 2017 indicates a signifi-
cant increase in the activity of fixation of at-
mospheric nitrogen during the pod filling stage.
The exception was the parameters of absolute
control, as well as controls, where a growth reg-
ulator was additionally applied in different stag-
es of soybeans development.
Significant growth of the nitrogen fixation
activity (+449 nmol C2H4 / plant per hour) dur-
ing the flowering stage was observed in the var-
iant with the plants treatment with the growth
regulator during the budding stage on the back-
ground of pre-sowing seeds treatment with the
same preparation compared to 306 nmol C2H4 /
/ plant per hour in a control variant with a simi-
lar treatment of plants.
The maximum values of nitrogen fixation
activity in pod filling stage were observed in the
variant with foliar application of growth regula-
tor in both stages on the background of Rhyzo-
humin used for seed treatment, where its growth
in comparison with the similar treatment of con-
trol plants (159 nmol C2H4 / plant per hour) was
6.1 fold.
As a result of the conducted study, it was
established that the soybeans yield was formed
depending on the weather conditions during the
years of study and application methods of plant
ISSN 1997-3004 Сільськогосподарська мікробіологія. — 2018. — Вип. 27.
57
growth regulator and microbial preparation use.
Thus, the conditions in 2016 were more favour-
able for the soybean production, as evidenced
by the average yield — 2.47 t/ha (Table 4).
Pre-sowing seed treatment with microbial
preparation used for optimization of nitrogen
nutrition of plants is an effective agrotechnical
technique used in modern agriculture. Overall,
inoculation of soybean seeds increased the yield
of grain by 0.21 t/ha (9.1 %) compared to
2.30 t/ha in absolute control.
Due to unfavourable weather conditions in
2017, soybean yield was relatively low (an av-
erage of 2.28 t/ha). However, even under these
conditions, the significant effect of separate ap-
plication of inoculation and growth regulator for
pre-sowing seed treatment on the formation of
the crop was noted. The yield from these treat-
ments was at a similar level of 2.29 t/ha, which
is 0.24 t/ha (11.7 %) higher compared to an ab-
solute control.
By estimating the average yield of soybeans
for two years, a clear (by 10.1 %) yields
increase compared to the control in the variant
using Rhyzohumin was noted. The use of the
growth regulator for seed treatment increased
this parameter by 9.2 %, while the simultaneous
use of both preparations — by 5.6 %.
According to individual studies [9; 10],
more tangible effects of growth regulators were
detected under their use as seeds treatment sim-
ultaneously with microbial preparations during
the vegetation period. Our study contradicts this
statement. Separately, by the years of study and
on average, the simultaneous pre-sowing seeds
treatment with Rhyzohumin and plant growth
regulator was less efficient in terms of yield
formation. At the same time, foliar application
of growth regulator during the budding and pod
filling stages at the background of seed treat-
ment with Rhyzohumin, resulted in 0.32 t/ha
(14.7 %) yield increase relatively to absolute
control (2.18 t/ha). We explain the reduction of
the studied parameters under the combined soy-
bean seeds inoculation with both preparations
compared with their effectiveness when applied
Table 4. The influence of microbial preparation Rhyzohumin and growth regulator Bioglo-
bine under the different methods of their application on the soybean yield of Siverka variety
Seed pro-
cessing (factor
А)
Plant processing with
Bioglobine solution by
vegetation (factor B)
Yield, t/ha Increment, t/ha
2016 2017 mean ± by factor А ± by factor B
Control
no treatment 2.30 2.05 2.18 К К
budding stage 2.32 2.14 2.23
0.06
grain filling stage 2.36 2.26 2.31 0.14
budding stage + grain
filling stage 2.40 2.30 2.35 0.18
Rhyzohumin
no treatment 2.51 2.29 2.40 0.23 К
budding stage 2.55 2.35 2.45
0.05
grain filling stage 2.57 2.36 2.47 0.06
budding stage + grain
filling stage 2.62 2.38 2.50 0.10
Bioglobine
no treatment 2.47 2.29 2.38 0.21 К
budding stage 2.50 2.32 2.41
0.03
grain filling stage 2.55 2.35 2.45 0.07
budding stage + grain
filling stage 2.51 2.34 2.43 0.04
Rhyzohumin +
Bioglobine
no treatment 2.41 2.21 2.31 0.14 К
budding stage 2.43 2.29 2.36
0.05
grain filling stage 2.47 2.32 2.40 0.09
budding stage + grain
filling stage 2.48 2.32 2.40 0.09
LSD05 for factor А
LSD05 for factor В
0.15
0.07
0.18
0.05
ISSN 1997-3004 Сільськогосподарська мікробіологія. — 2018. — Вип. 27.
58
separately with the possible overdose of physio-
logically active substances. At separate use of
preparations — pre-sowing seeds bacterization
with Rhyzohumin and foliar application of Bio-
globine, such overdose does not occur, as the
growth regulator is influencing not the seed or
seedling, but enhance the already formed leg-
ume-rhizobial nitrogen fixation system.
Consequently, the stage-by-stage applica-
tion of the studied factors: a microbial prepara-
tion as a seed treatment and a growth regulator
as a foliar treatment of vegetative plants during
the budding and pod filling stages has contribut-
ed the most to the increase of the productivity of
the symbiotic soybean-rhizobial system and
crop yield, while the combined seeds treatment
with Rhyzohumin and plant growth regulator
Bioglobine was shown to be the least effective
for the studied parameters.
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С. 43–45.
8. Іщенко В. А. Ефективність використання
Ризогуміну і Поліміксобактерину у поєднанні з
мікродобривом та регулятором росту при виро-
щуванні гороху вусатого типу в Північному Сте-
пу / В. А. Іщенко // Сільськогосподарська мікро-
біологія. — 2013. — Вип. 17. — С. 89–100.
9. Мишустин Е. Н. Биологическая фиксация
атмосферного азота / Е. Н. Мишустин, В. К. Шиль-
никова. — М. : Наука, 1968. — 531 с.
ВПЛИВ БІОПРЕПАРАТУ РИЗОГУМІНУ
ТА РЕГУЛЯТОРА РОСТУ РОСЛИН
БІОГЛОБІНУ НА СИМБІОТИЧНУ
АЗОТФІКСУВАЛЬНУ СИСТЕМУ І
ПРОДУКТИВНІСТЬ СОЇ
О. М. Мурач1, В. В. Волкогон2
1Інститут сільського господарства Північного
Сходу НААН, с. Сад, Сумський район,
Сумська область
2Інститут сільськогосподарської мікробіології
та агропромислового виробництва НААН,
м. Чернігів
Наведено результати досліджень впливу
передпосівної обробки насіння сої мікробним
препаратом Ризогуміном та регулятором
росту Біоглобіном, а також поетапного їх
застосування (Ризогумін для бактеризації
насіння, а рістстимулятор — по листу у
ВЛИЯНИЕ БИОПРЕПАРАТА
РИЗОГУМИНА И РЕГУЛЯТОРА
РОСТА РАСТЕНИЙ БИОГЛОБИНА
НА СИМБИОТИЧЕСКУЮ
АЗОТФИКСИРУЮЩУЮ СИСТЕМУ
И ПРОДУКТИВНОСТЬ СОИ
О. Н. Мурач1, В. В. Волкогон2
1Институт сельского хозяйства
Северо-Востока НААН, с. Сад, Сумской р-н,
Сумская обл.
2Институт сельскохозяйственной микробиологии
и агропромышленного производства НААН,
г. Чернигов
Приведены результаты исследований
влияния предпосевной обработки семян сои
микробным препаратом Ризогумином и ре-
гулятором роста Биоглобином, а также по-
этапного их применения (Ризогумин для
бактеризации семян, а ростстимулятор —
ISSN 1997-3004 Сільськогосподарська мікробіологія. — 2018. — Вип. 27.
59
різні фази органогенезу рослин) на форму-
вання і функціонування симбіотичного азо-
тфіксувального апарату в умовах північно-
східного Лісостепу України. Окреме засто-
сування для передпосівної обробки насіння
мікробного препарату та Біоглобіну інтен-
сивніше стимулює формування і активність
соєво-ризобіального симбіозу, ніж викорис-
тання препаратів у комплексі. Препарати
сприяють підвищенню зернової продуктив-
ності сої за умови їх поетапного застосу-
вання.
Ключові слова: соя, бактеризація, Ризо-
гумін, регулятор росту рослин Біоглобін, со-
єво-ризобіальний симбіоз, урожайність.
по листу в различные фазы органогенеза
растений) на формирование и функциониро-
вание симбиотического азотфиксирующего
аппарата в условиях северо-восточной Ле-
состепи Украины. Раздельное применение
для предпосевной обработки семян микроб-
ного препарата и Биоглобина более интен-
сивно стимулирует формирование и актив-
ность соево-ризобиального симбиоза, чем
использование препаратов в комплексе. Пре-
параты способствуют увеличению зерновой
продуктивности сои при поэтапном их при-
менении.
Ключевые слова: соя, бактеризация, Ри-
зогумин, регулятор роста растений Биогло-
бин, соево-ризобиальный симбиоз, урожай-
ность.
Received 20.01.2018
ISSN 1997-3004 Сільськогосподарська мікробіологія. — 2018. — Вип. 27.
|
| id | oai:ojs2.smic.in.ua:article-31 |
| institution | Agriciltural microbiology |
| keywords_txt_mv | keywords |
| language | English |
| last_indexed | 2026-07-23T01:02:15Z |
| publishDate | 2018 |
| publisher | Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine |
| record_format | ojs |
| resource_txt_mv | smicinua/9e/1779c91360a6276167334d77455e6c9e.pdf |
| spelling | oai:ojs2.smic.in.ua:article-312026-07-22T10:10:40Z INFLUENCE OF BIOPREPARATION RHYZOHUMIN AND PLANT GROWTH REGULATOR BIOGLOBINE ON SYMBIOTIC NITROGEN-FIXING SYSTEM AND YIELD OF SOYBEAN Murach, O. M. Volkohon, V. V. The paper presents the results of study on the influence of pre-sowing treatment of soybeanseeds with the microbial preparation Rhyzohumin and growth regulator Bioglobine, and their separate application (Rhyzohumin was used for seeds bacterization while growth regulator was appliedfoliar during different phases of plants organogenesis) on the formation and functioning of a symbiotic nitrogen-fixing apparatus in the conditions of the north-eastern forest-steppe of Ukraine.Separate application of studied preparations has intensively stimulated the formation and activityof soybean-rhizobial symbiosis, compared to their joint application. Considering their stage-bystage application the preparations has a positive action and increase the grain yield of soybeans. Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine 2018-07-06 Article Article Рецензована Стаття application/pdf https://smic.in.ua/index.php/journal/article/view/31 10.35868/1997-3004.27.52-59 Agricultural microbiology; Vol. 27 (2018): Agriciltural microbiology; 52-59 Сільськогосподарська мікробіологія; Том 27 (2018): Сільськогосподарська мікробіологія; 52-59 1997-3004 10.35868/1997-3004.27 en https://smic.in.ua/index.php/journal/article/view/31/28 Авторське право (c) 2018 O. M. Murach, V. V. Volkohon https://creativecommons.org/licenses/by/4.0 |
| spellingShingle | Murach, O. M. Volkohon, V. V. INFLUENCE OF BIOPREPARATION RHYZOHUMIN AND PLANT GROWTH REGULATOR BIOGLOBINE ON SYMBIOTIC NITROGEN-FIXING SYSTEM AND YIELD OF SOYBEAN |
| title | INFLUENCE OF BIOPREPARATION RHYZOHUMIN AND PLANT GROWTH REGULATOR BIOGLOBINE ON SYMBIOTIC NITROGEN-FIXING SYSTEM AND YIELD OF SOYBEAN |
| title_full | INFLUENCE OF BIOPREPARATION RHYZOHUMIN AND PLANT GROWTH REGULATOR BIOGLOBINE ON SYMBIOTIC NITROGEN-FIXING SYSTEM AND YIELD OF SOYBEAN |
| title_fullStr | INFLUENCE OF BIOPREPARATION RHYZOHUMIN AND PLANT GROWTH REGULATOR BIOGLOBINE ON SYMBIOTIC NITROGEN-FIXING SYSTEM AND YIELD OF SOYBEAN |
| title_full_unstemmed | INFLUENCE OF BIOPREPARATION RHYZOHUMIN AND PLANT GROWTH REGULATOR BIOGLOBINE ON SYMBIOTIC NITROGEN-FIXING SYSTEM AND YIELD OF SOYBEAN |
| title_short | INFLUENCE OF BIOPREPARATION RHYZOHUMIN AND PLANT GROWTH REGULATOR BIOGLOBINE ON SYMBIOTIC NITROGEN-FIXING SYSTEM AND YIELD OF SOYBEAN |
| title_sort | influence of biopreparation rhyzohumin and plant growth regulator bioglobine on symbiotic nitrogen-fixing system and yield of soybean |
| url | https://smic.in.ua/index.php/journal/article/view/31 |
| work_keys_str_mv | AT murachom influenceofbiopreparationrhyzohuminandplantgrowthregulatorbioglobineonsymbioticnitrogenfixingsystemandyieldofsoybean AT volkohonvv influenceofbiopreparationrhyzohuminandplantgrowthregulatorbioglobineonsymbioticnitrogenfixingsystemandyieldofsoybean |