ЕФЕКТИВНІСТЬ ПЕРЕДПОСІВНОЇ ІНОКУЛЯЦІЇ У ВИРОЩУВАННІ СІЛЬСЬКОГОСПОДАРСЬКИХ КУЛЬТУР ЗА РІЗНИХ ОРГАНІЧНИХ АГРОФОНІВ
Objective. Study the competitiveness, complementarity and symbiotic activity of a new strain of chickpea nodule bacteria Mesorhizobium ciceri ND-64, study the influence of seed inoculation on the productivity and yield of chickpea varieties of Ukrainian selection under their cultivation in different...
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| Дата: | 2021 |
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| Автори: | , , , , , , |
| Формат: | Стаття |
| Мова: | Англійська |
| Опубліковано: |
Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine
2021
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| Назва журналу: | Agriciltural microbiology |
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Agriciltural microbiology| _version_ | 1871466015391481856 |
|---|---|
| author | Сидоренко, В. П. Волкогон, В. В. Дімова, С. Б. Волкогон, К. І. Луценко, Н. В. Штанько, Н. П. Земська, І. А. |
| author_facet | Сидоренко, В. П. Волкогон, В. В. Дімова, С. Б. Волкогон, К. І. Луценко, Н. В. Штанько, Н. П. Земська, І. А. |
| author_institution_txt_mv | [
{
"author": "В. П. Сидоренко",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "В. В. Волкогон",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "С. Б. Дімова",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "К. І. Волкогон",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "Н. В. Луценко",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "Н. П. Штанько",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "І. А. Земська",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
}
] |
| author_sort | Сидоренко, В. П. |
| baseUrl_str | https://smic.in.ua/index.php/journal/oai |
| collection | OJS |
| datestamp_date | 2026-07-22T10:10:47Z |
| description | Objective. Study the competitiveness, complementarity and symbiotic activity of a new strain of chickpea nodule bacteria Mesorhizobium ciceri ND-64, study the influence of seed inoculation on the productivity and yield of chickpea varieties of Ukrainian selection under their cultivation in different soil and climatic zones of Ukraine. Methods. Serological, gas chromatography, field experiment and statistical. Results. The use of a new strain of M. ciceri ND-64 for pre-sowing bacterization of chickpea seeds of varieties Skarb, Admiral, Odysei, Budzhak, Triumf and Pamiat upon growing in field experiments in the Steppe zone against the background of the local population of nodule bacteria contributes to a significant increase in nitrogen-fixing nodules by 5 % to 67 %, an increase in the mass of nodules by 10 % to 67 % and nitrogenase activity 26 % to 150 % compared with the positive control (inoculation with the reference strain of M. ciceri H-12). The use of M. ciceri ND- 64 for pre-sowing bacterization of chickpea seeds under cultivation in the Steppe zone of Ukraine also had a positive effect on increasing the yield of chickpea — by 4 % to 18 % compared to the positive control. Inoculation with a new strain of chickpea seeds of varieties Skarb, Triumf and Pamiat contributed to the formation of higher number of nodules (by 57 % to 89 %), increaseed mass of nodules (2.2–2.9 times) and their nitrogenase activity (2–4 times) compared with the positive control upon cultivation in the Polissia zone on the fields where there is no population of chickpea rhizobia. Under these conditions, an increase in structural parameters of yield, the highest gain in chickpea yield, increase in photosynthetic activity and protein content in grain upon inoculation with M. ciceri ND-64 was shown. Obtaining a specific antisera by immunization of rabbits allowed to detect serological relatedness of M. ciceri ND-64, M. ciceri H-101, Mesorhizobium sp. ND-601 and M. ciceri ND-64, isolated from chickpea nodules of Skarb and Pamiat varieties. The competitiveness of M. ciceri ND-64 was determined using the immunological method in the field experiment. It was found that 100 % of nodules have been formed by the representatives of M. ciceri ND-64 in the variant where this serological group was applied for inoculation in the variant with inoculation of the strain M. ciceri ND-64 100 % of the nodules were formed by representatives of this serogroup. Conclusion. M. ciceri ND-64 strain is an effective microsymbiont of chickpea plants, due to its virulence, competitiveness, nitrogen-fixing properties. According to the results of field experiments, it was shown that the new strain, complementary to all studied varieties differing in seed size and shape, type of bush and average height, forms an effective symbiosis for growing chickpea plants both against the active local population of rhizobia and upon the absence of M. cicerі population in the soil. Symbiotic parameters upon seed treatment with suspension of M. ciceri ND-64 exceed these values in the variants with inoculation using the reference strain of M. ciceri H-12 and strains isolated from the nodules of each study variety. Therefore, M. ciceri ND-64 can be used as a bioagent of a microbial preparation for inoculation of chickpea of different varieties in order to form an effective bean-rhizobial symbiosis, increase the productivity of this culture and improve seed quality. |
| doi_str_mv | 10.35868/1997-3004.32.18-34 |
| first_indexed | 2025-07-17T12:25:35Z |
| format | Article |
| fulltext |
18
Сільськогосподарська мікробіологія. 2020. Вип. 32. С. 18–34.
ISSN 1997-3004
https://doi.org/10.35868/1997-3004.32.18-34
UDC 579.64:631.862.1:631.894:631.894
EFFICIENCY OF PRE-SOWING INOCULATION IN
CULTIVATION OF AGRICULTURAL CROPS UNDER
DIFFERENT ORGANIC AGRARIAN BACKGROUNDS
V. P. Sydorenko, V. V. Volkohon, S. B. Dimova, K. I. Volkohon, N. L. Lutsenko,
N. P. Shtanko, I. A. Zemska
Institute of Agricultural Microbiology and Agroindustrial Manufacture, NAAS
97 Shevchenka str., Chernihiv, 14035; Ukraine; e-mail: sydorenkochrom@gmail.com
Objective. Explore the influence of different organic agrarian backgrounds and their combina-
tions on the efficiency of pre-sowing seed inoculation and the development of introduced microor-
ganisms in the root spheres of cultivated plants. Methods. Microbiological, gas chromatography,
vegetation and field experiment, statistical. Results. In a long-term stationary field experiment on
leached chernozem, the stimulating effect and aftereffect of intermediate green manure (lupine) on
the manifestation of the efficiency of microbial preparations in the cultivation of potatoes, spring
barley, peas and winter wheat in crop rotation was established. The application of 5 t/ha of straw
had a weak effect on the efficiency of inoculation, but the combination of straw with green manure
provided a significant increase in yields. The positive effect of straw on the efficiency of pre-sowing
inoculation develops upon the aftereffects of this fertilizer. The application of 40 t/ha of cattle ma-
nure wiped out the efficiency of microbial preparations during the year of direct effect and the first
year aftereffect of fertilizer. The negative impact of cattle manure on the action of biological prepa-
rations has decreased over time. During the cultivation of crops on the background of “cattle ma-
nure + green manure”, the negative effect of cattle manure on the effect of inoculation was signifi-
cantly reduced. When the conditions of growing crops were simulated in the vegetation experiments
and upon the use of genetically-labelled bacterial strains — biological agents of microbial prepara-
tions, it was found that during the year of application of organic fertilizers, the development of
Azospirillum brasilense 410Str in root spheres of plants is positively influenced by lupine manure,
and cattle manure on the contrary results in significant reduction of the number of azospirilla. The
combination of green manure with cattle manure to some extent compensated for the negative im-
pact of the latter on the development of the introduced bacteria. During the first year aftereffects of
organic fertilizers, these features are maintained in the root zone of spring barley plants. When gro-
wing peas, the relationship between the formation of root nodules Rhizobium leguminosarum 31Str
and the second year aftereffect of organic fertilizers is not observed. When cultivating winter wheat
during the third year aftereffect, organic fertilizers have little effect on the development of Paeni-
bacillus polymyxa КВStr in the root spheres of plants. At the same time, in the variants of straw or
green manure aftereffects, parameters are slightly higher than the control ones. A slight negative
effect on the number of bacteria is still reported in the variant with the aftereffect of manure. Con-
clusion. The use of organic fertilizers can significantly affect the efficiency of pre-sowing inocula-
tion. The effect and aftereffect of cattle manure negatively affects the development of agronomically
beneficial bacteria introduced into the agrocenosis and significantly wipes out the positive effect of
biological preparations on crop yields. The use of microbial preparations for inoculation of seeds
of agricultural crops for their cultivation on the background of direct effect and aftereffect of inter-
mediate lupine green manure, including the combination of green manure with straw provides in-
tensive development of introduced microorganisms in root areas of plants and increased yields.
Keywords: inoculation, microbial preparations, straw, green manure, cattle manure, genetical-
ly-labelled bacterial strains.
© V. P. Sydorenko, V. V. Volkohon, S. B. Dimova, K. I. Volkohon, N. L. Lutsenko, N. P. Shtanko, I. A. Zemska, 2020
19
Introduction. Soil bacteria and microscop-
ic fungi play one of the essential roles in ensur-
ing the viability of cultivated plants. The plant,
provided with a full complex of microorgan-
isms, actively develops roots, gets easily digest-
ible nutrients and physiologically active com-
pounds from the soil, which has a positive effect
on its metabolism and production process as a
whole. However, due to significant distortions
in the application of certain agronomic tech-
niques (unreasonable levels of mineral fertiliz-
ers, pesticides, crop rotations, etc.), the compo-
sition of groups of microorganisms, which re-
quires appropriate correction is distorted in most
soils of agrocenoses [1–8]. The optimal solution
to this issue is the introduction of selected
strains of agronomically beneficial microorgan-
isms into agrocenoses using microbial prepara-
tions based on them.
Analysis of the novel studies and publica-
tions. Interest in the use of microorganisms in
technologies for growing crops increased in the
late 20th century due to the discovery of the
phenomenon of associative nitrogen fixation
and the ability of certain species of microbiota
to actively reproduce in the root spheres of
plants [9; 10]. Currently, in various research
centres, biological preparations have been de-
veloped based on selected active strains of mi-
croorganisms for most types of crops, including
non-leguminous [11–21]. Today, the production
of certain biological preparations has a com-
mercial basis.
The efficiency of microbial preparations
can be impressively high. Numerous field and
production experiments, studies in lysimetric
units, experiments using 15N, etc. have shown
that the efficiency of preparations by their im-
pact on the production process can be equivalent
to 30–60 kg/ha of mineral nitrogen, 15–30 kg/ha
of phosphorus [14; 23]. This is due to the in-
crease in the degree of assimilation of the active
substance from fertilizers and the improvement
of the constructive metabolism of plants, in
which mineral compounds in the plant body are
actively directed to the synthesis of organic sub-
stances [23–25].
A range of studies have convincingly
shown the efficiency of pre-sowing inoculation
of seeds in the cultivation of crops on mineral
agrarian backgrounds, especially low [26–29].
At the same time, the productivity of bacteriza-
tion by beneficial microorganisms under organic
agrarian backgrounds and their combinations
has been insufficiently studied. A typical feature
of existing publications is the statement about
the effective interaction between microorgan-
isms introduced into agrocenoses with organic
backgrounds. Thus, it was noted that the maxi-
mum diameter of the heads, parameters of their
raw and dry weight were registered during the
cultivation of lettuce inoculated with azospirilla
(GM1M1Az3) on the background of green ma-
nure and mulching [30]. It has also been report-
ed that the interaction between green manure
and inoculation of seeds with Herbaspirillum
seropedicae had a positive effect on maize
yield, contributing to the increased number of
grains and cob weight [31]. There is also evi-
dence of high efficiency of agronomically bene-
ficial bacteria used against the background of
cattle manure. For example, study by Lai et al.
[32] showed that although the growth of lettuce
in soil fertilized with swine manure when using
Azospirillum rugosum IMMIB AFH-6 was sig-
nificantly lower than when growing bacterized
plants in soil fertilized with chemical fertilizers,
but the variant “inoculation + animal manure +
1/2NPK” showed the highest yields and other
studied parameters. Studies conducted in Tur-
key [33] show that the use of Bacillus cereus,
Rhizobium rubi and Brevibacillus reuszeri when
growing broccoli on a background of animal
manure, contributed to an increase in crop
yields by 17.0 %, 20.2 % and 24.3 % and chlo-
rophyll content in leaves by 14.7 %, 14.0 % and
13.7 % compared to control (use of manure on-
ly), respectively. At the same time, the assimila-
tion of macronutrients and micronutrients by
plants increased. The use of manure and mem-
bers of the genus Azotobacter for inoculation
increased the biological yield of wheat grown in
the region of Elam (Iran) [34]. Dutta et al. also
reported an increase in maize yield with the use
of bacteria of the genus Azotobacter and 10 t/ha
of animal manure [35].
The review of the literature by Adesemoye
and Kloepper [36], when considering the im-
portance of agronomically useful microorgan-
isms to increase the assimilation of nutrients
from fertilizers by inoculate plants, does not dif-
ferentiate animal manure and chemical fertiliz-
ers as substrates containing chemical elements.
The authors consider promising the use of mi-
crobial preparations on the background of ani-
mal manure. At the same time, our previous
ISSN 1997-3004 Сільськогосподарська мікробіологія. 2020. Вип. 32.
20
studies on leached chernozem indicate low effi-
ciency of pre-sowing bacterization of crops
grown under the effect and aftereffects of cattle
manure [37]. Similar results were obtained by
T. Miliutenko [38]. However, the reasons for
this have not been clearly established yet.
Objective: to determine the efficiency of
microbial preparations in the cultivation of pota-
toes, spring barley, peas and winter wheat under
the effect and aftereffects of cattle manure, lu-
pine green manure, straw and their combina-
tions, to investigate the peculiarities of devel-
opment of microorganisms introduced into the
root zone of plants.
Materials and methods. Field studies were
conducted in 2017–2019 in a stationary experi-
ment of the Institute of Agricultural Microbiol-
ogy and Agroindustrial manufacture of the
NAAS on leached chernozem in conditions of
short-rotation crop rotation (potatoes – spring
barley – peas – winter wheat). The experiment
was started in 2009. Agrochemical characteris-
tics of the soil: pH — 5.3; humus content —
3.03 %; easily hydrolized nitrogen — 95 mg/kg
of soil; mobile phosphorus compounds (P2O5) —
150 (according to Kirsanov); the content of ex-
changeable potassium (K2O) (according to
Kirsanov) — 108 mg/kg of soil.
In the experiment, crops were grown ac-
cording to the scheme in 2 blocks — without
pre-sowing bacterization and with the use of
microbial preparations. Microbial preparations
used in the experiment — Biohran for potatoes
based on Azospirillum brasilense 410 (TU U
24.1-00497360-006:2009), Mikrohumin based
on A. brasilense 410 and physiologically active
substances of natural origin (TU U 24.1-
00497360-007:2009), Ryzohumin on the basis
of Rhizobium leguminosarum 31 (TU U 24.1-
00497360-003:2007) and Polimiksobakteryn on
the basis of phosphate-mobilizing bacteria Pae-
nibacillus polymyxa KB (TU U 24.1-00497360-
004:2009) are registered in the Ministry of En-
vironmental Protection of Ukraine and approved
for use.
Potatoes of the Skarbnytsia variety was
grown according to the following scheme:
1 — without fertilizers;
2 — straw;
3 — green manure;
4 — animal manure;
5 — straw + green manure;
6 — animal manure + green manure;
7 — N80Р80К80.
Spring barley of the Gosia variety was
grown according to the scheme (a — first year
aftereffect of organic matter):
1 — without fertilizers;
2 — strawа;
3 — green manureа;
4 — animal manureа;
5 — strawа + green manureа;
6 — animal manureа + green manureа;
7 — N60Р60К60.
Peas of Hotivsky variety was grown accord-
ing to the scheme (b — second year aftereffect
of organic matter):
1 — without fertilizers;
2 — strawb;
3 — green manureb;
4 — animal manureb;
5 — strawb + green manureb;
6 — animal manureb + green manureb;
7 — N30Р30К30.
Winter wheat of Poliska 90 variety was
grown according to the scheme (c — third year
aftereffect of organic matter):
1 — without fertilizers;
2 — strawc;
3 — green manurec;
4 — animal manurec;
5 — strawc + green manurec;
6 — animal manurec + green manurec;
7 — N60Р60К60.
Similar variants were provided in the block
of the experiment with bacterization.
Variants with the use of mineral fertilizers
were included in the scheme of the experiment
as a kind of control, for which high efficiency of
biological preparations was expected.
Fresh organic matter was added to the pota-
toes. Shredded straw in the amount of 5 t/ha was
placed into the soil immediately after harvesting
winter wheat (in crop rotation — the predeces-
sor of potatoes) by disking, and then intermedi-
ate green lupine was sown in the appropriate
variants. Green-manure mass of narrow-leaved
lupine (13–15 t/ha depending on the years of
study) was placed in the soil by disking fol-
lowed by shallow plowing (15 cm) in late au-
tumn (late November). At the same time, cattle
manure was applied into the soil at the rate of
40 t/ha in the relevant variants. Mineral fertiliz-
ers were applied each year according to the ex-
perimental scheme.
The area of the experimental plot is 43.2 m2
ISSN 1997-3004 Сільськогосподарська мікробіологія. 2020. Вип. 32.
21
(7.2 × 6.0), the repetition is 4 times.
In addition to field studies, in 2020 under
the conditions of vegetation experiments, the
degree of survival of bacteria, which are the
agents of relevant microbial preparations, in the
root spheres of plants were determined. 2.5 L
vessels were filled with 2 kg of soil each, the
humidity was maintained at 60 % of the total
moisture content.
The soil for vegetation experiments was se-
lected in the spring in the appropriate variants of
the field stationary experiment, the scheme of
which is described above. Therefore, organic
fertilizers were not introduced. Accordingly, the
soil for the experiment with potatoes contained
organic fertilizers applied since autumn, i. e.
their effect was direct. Soils for experiments
with barley, peas and wheat were characterized
by the first, second and third years aftereffects
of organic fertilizers, respectively. In the vari-
ants with mineral fertilizers, their appropriate
doses, equivalent to the field norms, were intro-
duced.
Bacteria used in the experiment were pre-
adapted by the resistance to streptomycin sul-
fate, which serves as a convenient genetic mark-
er. The method of spontaneously occurring mu-
tants by the antibiotic gradient in the digest me-
dium described by V. Zibalskyi was used [39].
The selected mutants were resistant to
3,000 μg/mL streptomycin sulfate doses and did
not differ from the parental forms in basic phys-
iological and biochemical parameters.
In the vegetation experiment with potatoes,
three fragments of potato tubers of 1 × 1 ×
× 2 cm3 with the same weight and one bud were
planted in the soil in each vessel. Upon planting,
each part of the tubers was treated with a sus-
pension of Azospirillum brasilense 410Str at a
rate of 500 thousand CFU/bud. After germina-
tion, the number of potato plants was reduced to
two per vessel.
In the experiment with barley, 10 seeds
were planted in the soil of each vessel. Before
planting, they were treated with a suspension of
Azospirillum brasilense 410Str at a rate of
225 thousand CFU/seed. After germination, the
number of seedlings was reduced to 5.
In the vegetation experiment with peas, 10
seeds were planted in each vessel. Before plant-
ing, they were treated with a suspension of
Rhizobium leguminosarum 31Str at a rate of
500 thousand CFU/seed. After germination, the
number of seedlings was reduced to 5.
Vegetation experiment with wheat involved
the use of a suspension of Paenibacillus poly-
myxa КВStr at a rate of 400 thousand CFU/seed.
Ten seeds were planted in each vessel, and after
the germination, the number of seedlings was
reduced to 5.
Repetition of experiments is three times.
Sampling of rhizosphere soil and washed
roots (as well as root nodules in the experiment
with peas) was carried out according to the ap-
propriate methods [40] over time, at Day 15, 30
and 45 after germination. The number of A. bra-
silense 410str was determined using the semi-
liquid medium Nfb [39] by the limiting dilutions
method. Additionally, an acetylene test was
used to establish positive (with a “+”) dilutions
[41]. Acetylene reduction activity was deter-
mined on a Chroom-5 gas chromatograph with a
flame ionization detector. The calculation of the
number of azospirilla was carried out by
McCready tables. To determine the number of
R. leguminosarum 31Str, an aliquot of the root
nodules of peas (each nodule separately) was
destroyed with a sterile glass rod in the sterile
test tubes with the addition of 1 mL of sterile
tap water, after which the resulting suspension
was sown on Petri dishes with pea agar (with
the addition of streptomycin sulphate
2.000 μg/mL). The dilution of rhizosphere soil
and washed wheat roots to detect P. polymyxa
KBStr was sown on MPA with streptomycin sul-
fate at a dose of 2,000 μg/mL.
The repletion of microbiological tests is
9 times.
The significance of differences between
variants was evaluated by Student’s t-test at
p ≤ 0.05. Test results are presented in the tables
using the smallest significant difference, in the
figures — as the arithmetic mean and the arith-
metic mean error.
Statistical processing of experimental data
was performed using Microsoft Office Excel
2003–2007.
Results and discussion.
Field experiments. Determining the de-
pendence of potato yield levels on fertilizer in-
dicates high efficiency of 40 t/ha of cattle ma-
nure (Table 1).
Significantly lower crop yields, although
with a significant gain in control parameters,
were observed with the use of lupine green ma-
nure. With the introduction of winter wheat
ISSN 1997-3004 Сільськогосподарська мікробіологія. 2020. Вип. 32.
22
Table 1. Influence of fertilization and inoculation on potato yield
Variants of experiments
Yield (average
for three years)
Gain from fertilizers
and inoculation Gain from inoculation
t/ha
Without inoculation
Without fertilizers (control) 13.0 – –
Straw 13.4 0.4 –
Green manure 14.6 1.6 –
Animal manure 22.8 9.8 –
Straw + green manure 16.2 3.2 –
Animal manure + green manure 24.0 11.0 –
N80P80K80 24.6 11.6 –
With Biohran
Without fertilizers 14.7 – 1.7
Straw 14.3 1.3 0.9
Green manure 16.5 3.5 1.9
Animal manure 23.1 10.1 0.3
Straw + green manure 19.7 6.7 3.5
Animal manure + green manure 25.9 12.9 1.9
N80P80K80 26.1 13.1 1.5
НІР05 1.1 – –
straw, we expected a decrease in potato yield
due to the possible immobilization of mineral
nitrogen compounds in the soil, but the data in-
dicate no intensive development of this pro-
cess — yield in this case was at the control lev-
el. This can be explained by the fact that under
the conditions of a long-term stationary field
experiment, the processes of biological trans-
formation of nitrogen compounds were balan-
ced. The combination of straw with green ma-
nure provided a significant increase in crop
yields. Lupine green manure helped to increase
productivity also in combination with animal
manure. Thus, green manure provided an in-
crease in the effective fertility of leached cher-
nozem.
Potato yields when mineral fertilizers were
introduced to the soil, were practically at the
level of the “animal manure + green manure”
variant.
The efficiency of Biohran depended on the
characteristics of the crop fertilization. A signif-
icant increase in inoculation parameters was re-
ported in the variant without fertilizers. The
predicted increase in yield was obtained by
growing inoculated plants on the background of
N80P80K80. Under the exposure to 5 t/ha of straw
and Biohran, a tendency to increase crop pro-
ductivity only was noted. The effect of the prep-
aration on the formation of yields when growing
potatoes on a green manure background should
be clearly emphasized. At the same time, the ef-
ficiency of potato inoculation was higher
against the background of green manure with
straw. When growing the crop on the back-
ground of animal manure, the efficiency of the
biological preparation was smoothed down, but
the cultivation of inoculated plants under com-
bining animal manure with lupine green manure
provided a significant increase in crop yield.
The first year aftereffect of organic fertiliz-
ers generally had a positive effect on the yield
of the next crop in the crop rotation — spring
barley (Table 2). However, the straw aftereffect
showed only a tendency towards the increase
yields. A significant increase was reported in
straw plus lupine green manure aftereffect. As
with potato growing, this combination contrib-
uted to higher yields than with each of these fer-
tilizers individually.
The yield of barley increased by 1.33 t/ha
under animal manure aftereffects, but against
ISSN 1997-3004 Сільськогосподарська мікробіологія. 2020. Вип. 32.
23
Table 2. Influence of fertilization and inoculation on spring barley yield
Variants of experiment
Yield (average
for three years)
Gain from fertilizers
and inoculation Gain from inoculation
t/ha
Without inoculation
Without fertilizers (control) 2.07 – –
Strawа 2.21 0.14 –
Green manureа 2.41 0.34 –
Animal manureа 3.40 1.33 –
Strawа + green manureа 2.67 0.60 –
Animal manureа + green manureа 3.64 1.57 –
N60P60K60 2.94 0.87 –
With Mikrohumin
Without fertilizers 2.53 0.46 0.46
Strawа 2.69 0.62 0.48
Green manureа 2.90 0.83 0.49
Animal manureа 3.64 1.57 0.24
Strawа + green manureа 3.25 1.18 0.58
Animal manureа + green manureа 4.02 1.95 0.38
N60P60K60 3.40 1.33 0.46
НІР05 0.21 – –
the background of the aftereffect of this fertiliz-
er applied together with green manure, an in-
crease of 1.57 t/ha was obtained.
The direct impact of mineral fertilizers on
the productivity of the agrocenosis was at the
level of 0.87 t/ha.
The use of Mikrohumin for pre-sowing in-
oculation of seeds contributed to a significant
increase in barley yield for all studied agrarian
backgrounds, but the lowest rate in the experi-
ment was observed in the first year aftereffect of
cattle manure.
When cultivating peas based on the second
year aftereffect of organic fertilizers, a positive
effect of all agrarian backgrounds on crop yields
was reported. Furthermore, the aftereffect of
5 t/ha of straw provided a significant increase in
yield (Table 3).
The aftereffect of cattle manure was almost
at the level of the aftereffect of lupine green
manure, but the effect of the combined applica-
tion of these two types of organic fertilizers was
much higher than the effect of each of them
separately.
The highest yields in the experiment were
obtained by direct action of N30P30K30.
The efficiency of Ryzohumin, used for pre-
sowing inoculation of pea seeds, was almost at
the same level for all organic agrarian back-
grounds. It was slightly higher under the direct
action of N30P30K30 (Table 3).
Under the third year aftereffects of organic
fertilizers, their influence on the formation of
winter wheat yield was insignificant and was
within the statistical error of the results (Tab-
le 4). The only exception was the aftereffect
of animal manure and lupine green manure ap-
plied together, which provided a yield increase
of 0.28 t/ha. The highest parameters of crop
yield in the experiment were obtained by direct
action of mineral fertilizers at the rate of
N60P60K60.
The positive effect of the microbial prepara-
tion Polimiksobakteryn on the yield of winter
wheat was manifested in all variants of the ex-
periment, but when growing the crop in the third
year aftereffect of organic fertilizers, it was
much smaller than under direct action of miner-
al fertilizers. There was also no significant dif-
ference in the efficiency of the biological prepa-
ration in the cultivation of the crop under the af-
tereffects of organic fertilizers.
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24
Table 3. Influence of fertilization and bacterization on pea yield
Variants of experiment
Yield (average
for three years)
Gain from fertilizers
and inoculation Gain from inoculation
t/ha
Without inoculation
Without fertilizers (control) 2,16 – –
Strawb 2.33 0.17 –
Green manureb 2.38 0.22 –
Animal manureb 2.44 0.28 –
Strawb + green manureb 2.38 0.22 –
Animal manureb + green manureb 2.58 0.42 –
N30P30K30 2.70 0.54 –
With Ryzohumin
Without fertilizers 2.48 0.32 0.32
Strawb 2.63 0.47 0.30
Green manureb 2.73 0.57 0.35
Animal manureb 2.78 0.62 0.34
Strawb + green manureb 2.77 0.61 0.39
Animal manureb + green manureb 2.94 0.78 0.36
N30P30K30 3.19 1.03 0.49
НІР05 0.16 – –
The obtained results of the influence of mi-
crobial preparations on the formation of crop
yields during their cultivation upon organic
backgrounds indicate the ambiguous nature of
the manifestation of the efficiency of microbio-
logical factors. Thus, the positive effect of bio-
logical preparations is smoothed out at the year
of direct action of cattle manure and is gradually
restored over time under the aftereffects of this
factor.
We attribute this to the fact that a huge
number of microorganisms enter the soil along
with animal manure [42; 43], which can create a
strong competitive environment for the agro-
nomically useful microorganism introduced into
the agrocenosis. Although animal manure mi-
croorganisms are represented by many species
of bacteria and micromycetes, including those
that are not characteristic of effective interaction
with plants, they can provide high competition
for the substrate and occupy free ecological
niches and create unfavourable conditions for
survival in the root spheres of bacterial plants
which is the basis of the studied biological
preparations. We have previously [37] hypothe-
sized non-specific bacterization of the soil with
the introduction of animal manure, which may
prevent the successful introduction of agronomi-
cally beneficial microorganisms in agrocenoses.
Another situation is registered upon the cul-
tivation of crops on the background of lupine
green manure. The biomass of green manure is
relatively weakly contaminated with microbiota
and, in our opinion, does not interfere with the
successful introduction of agronomically valua-
ble bacteria. At the same time, green manure
contains a significant amount of water-soluble
organic substances, proteins and starch [44],
which are rapidly mineralized and can addition-
ally provide microorganisms with easily digest-
ible organic compounds. Also, this organic ferti-
lizer has a positive effect on plant nutrition, due
to which they increase the root system and the
production of root exudates [45], which can
stimulate the reproduction of microorganisms.
This is confirmed by the improvement of
the situation when combining green manure
with straw and even animal manure.
Straw as a fertilizer has little effect on the
efficiency of pre-sowing inoculation, which
confirms the well-known thesis that it rather has
influence on the potential fertility of the soil
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25
Table 4. Influence of fertilization and bacterization on wheat yield
Variants of experiment
Yield (average
for three years)
Gain from fertilizers
and inoculation Gain from inoculation
t/ha
Without inoculation
Without fertilizers (control) 3.92 – –
Strawс 3.95 0.03 –
Green manureс 4.04 0.12 –
Animal manureс 4.12 0.20 –
Strawс + green manureс 4.03 0.11 –
Animal manureс + green manureс 4.20 0.28 –
N60P60K60 4.42 0.50 –
With Polimiksobakteryn
Without fertilizers (control) 4.24 0.32 0.32
Strawс 4.35 0.43 0.40
Green manureс 4.53 0.61 0.49
Animal manureс 4.42 0.50 0.30
Strawс + green manureс 4.43 0.51 0.40
Animal manureс + green manureс 4.62 0.70 0.42
N60P60K60 5.30 1.38 0.88
НІР05 0.21 – –
(due to the high content of carbon and the
possibility of its sequestration) than on the ef-
fective one. At the same time, the use of straw
can affect the efficiency of biological prepara-
tions to some extent through the impact on the
processes of biological transformation of nitro-
gen compounds.
Both positive and negative effects of organ-
ic fertilizers fade over time, which is quite logi-
cal given the changes in the state of the micro-
biota in the soil, and due to the gradual shortage
of nutrients.
Study of the survival of biological agents
of microbial preparations in root spheres of
plants. To clarify some assumptions about the
influence of organic agrarian backgrounds on
the survival of agronomically useful microor-
ganisms in the root spheres of plants, studies
were conducted under the conditions of vegeta-
tion experiments.
Studies of the development of A. brasilense
410Str in the root spheres of potato plants have
revealed significant differences between the var-
iants. The development of the introduced mi-
croorganism in the rhizosphere soil is the largest
under the mineral agrarian background (Fig. 1).
Compared to other variants of the experiment,
azospirilla develop more intensively under the
action of lupine green manure, as well as when
combining green manure with winter wheat
straw.
The lowest number of the studied bacteria
in the rhizosphere soil were observed when
growing potatoes against the background of cat-
tle manure.
The number of azospirilla during the culti-
vation of plants on a background of straw has
almost no difference from the control parame-
ters. We explain this by the fact that in the year
of straw application, the processes of its miner-
alization are only beginning to develop and do
not significantly affect the number of A. bra-
silense 410Str.
The dependence of A. brasilense 410Str de-
velopment from the agrarian background on the
roots of potato plants has a much more con-
trasting appearance (Fig. 2). Azospirilla develop
intensively on the roots under a mineral agrarian
background, however the highest parameters of
the number of bacteria are noted under the cul-
tivation of plants on a green manure back-
ground. Whilst they are the highest in the exper-
ISSN 1997-3004 Сільськогосподарська мікробіологія. 2020. Вип. 32.
26
W/o fert.: without fertilizer; G.m.: green manure; A.m.: animal manure
Fig. 1. Number of A. brasilense 410Str in rhizosphere soil of potato plants depending on the
agrarian background.
W/o fert.: without fertilizer; G.m.: green manure; A.m.: animal manure
Fig. 2. Development of A. brasilense 410Str on the roots of potato plants depending on the
agrarian background.
iment. Introduced azospirilla are extremely
poorly developed on the roots of plants grown
on the background of cattle manure. No signifi-
cant difference was observed between the pa-
rameters of the number of A. brasilense 410Str
upon growing potatoes in the control variant and
on the background of straw both in the rhizo-
sphere soil, and on the roots of plants. At the
same time, when combining straw with lupine
green manure, the intensity of development of
azospirilla increases significantly.
The reported peculiarities of A. brasilense
410Str development in the root spheres of potato
plants are largely maintained in the experiment
with spring barley, where the first year afteref-
fect of organic fertilizers on the survival of
azospirilla was studied. The highest parameters
of the number of streptomycin-resistant azospi-
rilla in the rhizosphere soil of barley plants were
observed for growing plants on a mineral back-
ground (Fig. 3). Relatively high intensity of
A. brasilense 410Str development is observed in
the first year aftereffect of lupine green manure,
including the combination of this fertilizer with
straw.
The lowest values of streptomycin-resistant
azospirilla were observed upon the aftereffects
of cattle manure.
The development of A. brasilense 410Str on
the roots of barley plants also depended on the
agrarian background (Fig. 4). The highest val-
ues were reported under the cultivation of plants
on the mineral background, as well as under the
aftereffects of green manure, including combi-
nation with wheat straw. Variants with cattle
manure are still unfavourable for the develop-
ment of Azospirillum brasilense 410Str. A slight
positive effect is observed from the aftereffect
of wheat straw.
In the vegetation experiment with peas,
which studied the effect of the second year af-
tereffects of organic fertilizers on the formation
of nitrogen-fixing nodules with the involvement
of Rh. leguminosarum 31Str we have found no
0
15
30
45
60
75
90
W/o fert. Straw G.m. A.m. Straw+g.m. A.m.+g.m. N80Р80К80
th
ou
sa
nd
p
er
1
g
o
f d
ry
s
oi
l
Day 15 Day 30 Day 45
0
4
8
12
16
20
24
28
W/o fert. Straw G.m. A.m. Straw+g.m. A.m+g.m. N80Р80К80
m
ln
p
er
1
g
o
f d
ry
ro
ot
Day 15 Day 30 Day 45
N80P80K80
N80P80K80
ISSN 1997-3004 Сільськогосподарська мікробіологія. 2020. Вип. 32.
27
W/o fert.: without fertilizer; G.m.: green manure; A.m.: animal manure
Fig. 3. Number of A. brasilense 410Str in rhizosphere soil of spring barley depending on the
agrarian background.
W/o fert.: without fertilizer; G.m.: green manure; A.m.: animal manure
Fig. 4. Development of A. brasilense 410Str on the roots of spring barley depending on the
agrarian background.
significant differences between the variants.
This can be explained both by a decrease in the
effect of organic fertilizers on the efficiency of
bacterial introduction over time, and (or) the
lack of significant competition between micro-
organisms in the formation of nitrogen-fixing
symbioses. These studies should be continued
using other methods, including serological.
In the vegetation experiment with winter
wheat upon the third year aftereffect of organic
fertilizers, a significant smoothing up of both
the negative impact of cattle manure and the
positive effect of lupine green manure on the
development of P. polymyxa KBStr in the root
spheres was registered (Fig. 5 and 6). At the
same time, the parameters of variants with green
manure still exceed the value of control, which
indicates its positive impact on the development
of plant-microbial interactions even in the
fourth year aftereffect. A slight negative impact
on the studied parameter is still observed in the
variant with animal manure.
Conclusion. The efficiency of microbial
preparations in the system of organic farming
largely depends on agrarian backgrounds. The
use of cattle manure offsets the positive effect
of inoculation in the year of direct action of the
fertilizer and in the first year aftereffect, due to
the low level of survival of the microorganism
introduced into the agrocenosis in the root
spheres of plants under these conditions. Over
time, the negative effect of animal manure on
the efficiency of biological preparations de-
creases. The use of green manure (narrow-
leaved lupine) for fertilization helps to increase
the efficiency of pre-sowing inoculation both in
the year of direct action of this fertilizer and un-
der its aftereffects. To reduce the negative im-
pact of litter manure on the efficiency of micro-
bial preparations, it is proposed to use lupine
green manure together with animal manure.
This is confirmed both by the level of crop yield
and by studies of the survival of microorgan-
isms introduced into agrocenoses in the root
0
15
30
45
60
75
90
105
W/o fert. Straw G.m. A.m. Straw+g.m. A.m.+g.m. N60Р60К60
th
ou
sa
nd
p
er
1
g
o
f d
ry
s
oi
l
Day 15 Day 30 Day 45
0
3
6
9
12
15
W/o fert. Straw G.m. A.m. Straw+g.m. A.m.+g.m. N60Р60К60
m
ln
p
er
1
g
o
f d
ry
ro
ot
Day 15 Day 30 Day 45
ISSN 1997-3004 Сільськогосподарська мікробіологія. 2020. Вип. 32.
N60P60K60
N60P60K60
28
W/o fert.: without fertilizer; G.m.: green manure; A.m.: animal manure
Fig. 5. Number of P. polymyxa КВStr in rhizosphere soil of winter wheat plants upon different
agrarian backgrounds
W/o fert.: without fertilizer; G.m.: green manure; A.m.: animal manure
Fig. 6. Development of P. polymyxa КВStr on the roots of winter wheat depending on the agrar-
ian background
spheres of plants. The use of straw as a fertilizer
does not significantly affect the efficiency of
microbial preparations upon the first and second
year aftereffects. Over time, the influence of
straw on the efficiency of inoculants increases.
To increase the effect of biological preparations
on crop productivity, the combination of straw
with green manure deserves attention.
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Received 06.10.2020
https://doi.org/10.35868/1997-3004.32.18-34
УДК 579.64:631.862.1:631.894:631.894
ЕФЕКТИВНІСТЬ ПЕРЕДПОСІВНОЇ ІНОКУЛЯЦІЇ
У ВИРОЩУВАННІ СІЛЬСЬКОГОСПОДАРСЬКИХ КУЛЬТУР
ЗА РІЗНИХ ОРГАНІЧНИХ АГРОФОНІВ
В. П. Сидоренко, В. В. Волкогон, С. Б. Дімова, К. І. Волкогон, Н. В. Луценко,
Н. П. Штанько, І. А. Земська
Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН, м. Чернігів
e-mail: sydorenkochrom@gmail.com
Мета. Дослідити вплив різних органічних агрофонів та їх поєднань на ефективність пе-
редпосівної інокуляції насіння та розвиток інтродукованих мікроорганізмів у кореневих сфе-
рах культурних рослин. Методи. Мікробіологічні, газохроматографічний, вегетаційного та
польового дослідів, статистичні. Результати. У тривалому польовому стаціонарному дос-
ліді на чорноземі вилуженому встановлено стимулювальну дію та післядію проміжного си-
дерату (люпин вузьколистий) на прояв ефективності мікробних препаратів за вирощування
картоплі, ячменю ярого, гороху та пшениці озимої в сівозміні. Застосування 5 т/га соломи
слабо впливало на ефективність інокуляції, проте поєднання соломи із зеленим добривом за-
безпечувало суттєве зростання показників урожайності. Позитивний вплив соломи на ефе-
ктивність передпосівної інокуляції проявляється за післядії цього добрива. Внесення 40 т/га
підстилкового гною великої рогатої худоби (ВРХ) нівелювало ефективність мікробних пре-
паратів у рік прямої дії та першого року післядії добрива. З часом негативний вплив гною на
дію біопрепаратів зменшувався. За вирощування сільськогосподарських культур по фону
«гній + сидерат» негативна дія гною на вплив інокуляції суттєво знижувалася. За моделю-
вання умов вирощування сільськогосподарських культур у вегетаційних дослідах та викори-
стання генетично маркованих штамів бактерій — біологічних агентів мікробних препара-
тів — встановлено, що в рік внесення органічних добрив на розвиток Azospirillum brasilense
410Str у кореневих сферах рослин позитивно впливає люпиновий сидерат, а використання під-
стилкового гною ВРХ — навпаки, призводить до суттєвого зменшення чисельності азоспі-
рил. Поєднання зеленого добрива з гноєм ВРХ деякою мірою компенсувало негативний вплив
останнього на розвиток інтродукованої бактерії. В умовах першого року післядії органічних
добрив в кореневій зоні рослин ячменю ярого ці особливості зберігаються. За вирощування
гороху залежність між утворенням кореневих бульбочок Rhizobium leguminosarum 31Str та
другого року післядії органічних добрив не простежується. За вирощування пшениці озимої в
умовах третього року післядії органічні добрива незначною мірою впливають на розвиток
Paenibacillus polymyxa КВStr у кореневих сферах рослин. Водночас у варіантах післядії соломи
або сидерату показники дещо перевершують контрольні. Незначний негативний вплив на
чисельність бактерії все ще проявляється у варіанті з післядією гною. Висновки. Викорис-
тання органічних добрив може суттєво впливати на ефективність передпосівної інокуляції.
Дія та післядія підстилкового гною ВРХ негативно впливає на розвиток інтродукованих в
агроценоз агрономічно корисних бактерій і значною мірою нівелює позитивний вплив біопре-
паратів на урожайність культур. Застосування мікробних препаратів для інокуляції насіння
ISSN 1997-3004 Сільськогосподарська мікробіологія. 2020. Вип. 32.
32
сільськогосподарських культур за їх вирощування по фону прямої дії та післядії проміжного
люпинового сидерату, у т. ч. й за поєднання зеленого добрива з соломою, забезпечує інтен-
сивний розвиток інтродукованих мікроорганізмів в кореневих сферах рослин і зростання
урожайності.
Ключові слова: інокуляція, мікробні препарати, солома, сидерат, гній ВРХ, генетично
марковані штами бактерій.
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| language | English |
| last_indexed | 2026-07-23T01:11:12Z |
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| spelling | oai:ojs2.smic.in.ua:article-3392026-07-22T10:10:47Z EFFICIENCY OF PRE-SOWING INOCULATION IN CULTIVATION OF AGRICULTURAL CROPS UNDER DIFFERENT ORGANIC AGRARIAN BACKGROUNDS ЕФЕКТИВНІСТЬ ПЕРЕДПОСІВНОЇ ІНОКУЛЯЦІЇ У ВИРОЩУВАННІ СІЛЬСЬКОГОСПОДАРСЬКИХ КУЛЬТУР ЗА РІЗНИХ ОРГАНІЧНИХ АГРОФОНІВ Сидоренко, В. П. Волкогон, В. В. Дімова, С. Б. Волкогон, К. І. Луценко, Н. В. Штанько, Н. П. Земська, І. А. bean-rhizobial symbiosis, nodular bacteria, Mesorhizobium ciceri, chickpea інокуляція, мікробні препарати, солома, сидерат, гній ВРХ, генетично марковані штами бактерій Objective. Study the competitiveness, complementarity and symbiotic activity of a new strain of chickpea nodule bacteria Mesorhizobium ciceri ND-64, study the influence of seed inoculation on the productivity and yield of chickpea varieties of Ukrainian selection under their cultivation in different soil and climatic zones of Ukraine. Methods. Serological, gas chromatography, field experiment and statistical. Results. The use of a new strain of M. ciceri ND-64 for pre-sowing bacterization of chickpea seeds of varieties Skarb, Admiral, Odysei, Budzhak, Triumf and Pamiat upon growing in field experiments in the Steppe zone against the background of the local population of nodule bacteria contributes to a significant increase in nitrogen-fixing nodules by 5 % to 67 %, an increase in the mass of nodules by 10 % to 67 % and nitrogenase activity 26 % to 150 % compared with the positive control (inoculation with the reference strain of M. ciceri H-12). The use of M. ciceri ND- 64 for pre-sowing bacterization of chickpea seeds under cultivation in the Steppe zone of Ukraine also had a positive effect on increasing the yield of chickpea — by 4 % to 18 % compared to the positive control. Inoculation with a new strain of chickpea seeds of varieties Skarb, Triumf and Pamiat contributed to the formation of higher number of nodules (by 57 % to 89 %), increaseed mass of nodules (2.2–2.9 times) and their nitrogenase activity (2–4 times) compared with the positive control upon cultivation in the Polissia zone on the fields where there is no population of chickpea rhizobia. Under these conditions, an increase in structural parameters of yield, the highest gain in chickpea yield, increase in photosynthetic activity and protein content in grain upon inoculation with M. ciceri ND-64 was shown. Obtaining a specific antisera by immunization of rabbits allowed to detect serological relatedness of M. ciceri ND-64, M. ciceri H-101, Mesorhizobium sp. ND-601 and M. ciceri ND-64, isolated from chickpea nodules of Skarb and Pamiat varieties. The competitiveness of M. ciceri ND-64 was determined using the immunological method in the field experiment. It was found that 100 % of nodules have been formed by the representatives of M. ciceri ND-64 in the variant where this serological group was applied for inoculation in the variant with inoculation of the strain M. ciceri ND-64 100 % of the nodules were formed by representatives of this serogroup. Conclusion. M. ciceri ND-64 strain is an effective microsymbiont of chickpea plants, due to its virulence, competitiveness, nitrogen-fixing properties. According to the results of field experiments, it was shown that the new strain, complementary to all studied varieties differing in seed size and shape, type of bush and average height, forms an effective symbiosis for growing chickpea plants both against the active local population of rhizobia and upon the absence of M. cicerі population in the soil. Symbiotic parameters upon seed treatment with suspension of M. ciceri ND-64 exceed these values in the variants with inoculation using the reference strain of M. ciceri H-12 and strains isolated from the nodules of each study variety. Therefore, M. ciceri ND-64 can be used as a bioagent of a microbial preparation for inoculation of chickpea of different varieties in order to form an effective bean-rhizobial symbiosis, increase the productivity of this culture and improve seed quality. Мета. Дослідити вплив різних органічних агрофонів та їх поєднань на ефективність передпосівної інокуляції насіння та розвиток інтродукованих мікроорганізмів у кореневих сферах культурних рослин. Методи. Мікробіологічні, газохроматографічний, вегетаційного тапольового дослідів, статистичні. Результати. У тривалому польовому стаціонарному досліді на чорноземі вилуженому встановлено стимулювальну дію та післядію проміжного сидерату (люпин вузьколистий) на прояв ефективності мікробних препаратів за вирощуваннякартоплі, ячменю ярого, гороху та пшениці озимої в сівозміні. Застосування 5 т/га соломислабо впливало на ефективність інокуляції, проте поєднання соломи із зеленим добривом забезпечувало суттєве зростання показників урожайності. Позитивний вплив соломи на ефективність передпосівної інокуляції проявляється за післядії цього добрива. Внесення 40 т/гапідстилкового гною великої рогатої худоби (ВРХ) нівелювало ефективність мікробних препаратів у рік прямої дії та першого року післядії добрива. З часом негативний вплив гною надію біопрепаратів зменшувався. За вирощування сільськогосподарських культур по фону«гній + сидерат» негативна дія гною на вплив інокуляції суттєво знижувалася. За моделювання умов вирощування сільськогосподарських культур у вегетаційних дослідах та використання генетично маркованих штамів бактерій — біологічних агентів мікробних препаратів — встановлено, що в рік внесення органічних добрив на розвиток Azospirillum brasilense410Str у кореневих сферах рослин позитивно впливає люпиновий сидерат, а використання підстилкового гною ВРХ — навпаки, призводить до суттєвого зменшення чисельності азоспірил. Поєднання зеленого добрива з гноєм ВРХ деякою мірою компенсувало негативний впливостаннього на розвиток інтродукованої бактерії. В умовах першого року післядії органічнихдобрив в кореневій зоні рослин ячменю ярого ці особливості зберігаються. За вирощуваннягороху залежність між утворенням кореневих бульбочок Rhizobium leguminosarum 31Str тадругого року післядії органічних добрив не простежується. За вирощування пшениці озимої вумовах третього року післядії органічні добрива незначною мірою впливають на розвитокPaenibacillus polymyxa КВStr у кореневих сферах рослин. Водночас у варіантах післядії соломиабо сидерату показники дещо перевершують контрольні. Незначний негативний вплив начисельність бактерії все ще проявляється у варіанті з післядією гною. Висновки. Використання органічних добрив може суттєво впливати на ефективність передпосівної інокуляції.Дія та післядія підстилкового гною ВРХ негативно впливає на розвиток інтродукованих вагроценоз агрономічно корисних бактерій і значною мірою нівелює позитивний вплив біопрепаратів на урожайність культур. Застосування мікробних препаратів для інокуляції насіннясільськогосподарських культур за їх вирощування по фону прямої дії та післядії проміжноголюпинового сидерату, у т. ч. й за поєднання зеленого добрива з соломою, забезпечує інтенсивний розвиток інтродукованих мікроорганізмів в кореневих сферах рослин і зростанняурожайності. Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine 2021-01-04 Article Article Рецензована Стаття application/pdf https://smic.in.ua/index.php/journal/article/view/339 10.35868/1997-3004.32.18-34 Agricultural microbiology; Vol. 32 (2021): Agriciltural microbiology; 18-34 Сільськогосподарська мікробіологія; Том 32 (2021): Сільськогосподарська мікробіологія; 18-34 1997-3004 10.35868/1997-3004.32 en https://smic.in.ua/index.php/journal/article/view/339/424 Авторське право (c) 2021 V. P. Sydorenko, V. V. Volkohon, S. B. Dimova, K. I. Volkohon, N. L. Lutsenko, N. P. Shtanko, I. A. Zemska https://creativecommons.org/licenses/by/4.0 |
| spellingShingle | інокуляція мікробні препарати солома сидерат гній ВРХ генетично марковані штами бактерій Сидоренко, В. П. Волкогон, В. В. Дімова, С. Б. Волкогон, К. І. Луценко, Н. В. Штанько, Н. П. Земська, І. А. ЕФЕКТИВНІСТЬ ПЕРЕДПОСІВНОЇ ІНОКУЛЯЦІЇ У ВИРОЩУВАННІ СІЛЬСЬКОГОСПОДАРСЬКИХ КУЛЬТУР ЗА РІЗНИХ ОРГАНІЧНИХ АГРОФОНІВ |
| title | ЕФЕКТИВНІСТЬ ПЕРЕДПОСІВНОЇ ІНОКУЛЯЦІЇ У ВИРОЩУВАННІ СІЛЬСЬКОГОСПОДАРСЬКИХ КУЛЬТУР ЗА РІЗНИХ ОРГАНІЧНИХ АГРОФОНІВ |
| title_alt | EFFICIENCY OF PRE-SOWING INOCULATION IN CULTIVATION OF AGRICULTURAL CROPS UNDER DIFFERENT ORGANIC AGRARIAN BACKGROUNDS |
| title_full | ЕФЕКТИВНІСТЬ ПЕРЕДПОСІВНОЇ ІНОКУЛЯЦІЇ У ВИРОЩУВАННІ СІЛЬСЬКОГОСПОДАРСЬКИХ КУЛЬТУР ЗА РІЗНИХ ОРГАНІЧНИХ АГРОФОНІВ |
| title_fullStr | ЕФЕКТИВНІСТЬ ПЕРЕДПОСІВНОЇ ІНОКУЛЯЦІЇ У ВИРОЩУВАННІ СІЛЬСЬКОГОСПОДАРСЬКИХ КУЛЬТУР ЗА РІЗНИХ ОРГАНІЧНИХ АГРОФОНІВ |
| title_full_unstemmed | ЕФЕКТИВНІСТЬ ПЕРЕДПОСІВНОЇ ІНОКУЛЯЦІЇ У ВИРОЩУВАННІ СІЛЬСЬКОГОСПОДАРСЬКИХ КУЛЬТУР ЗА РІЗНИХ ОРГАНІЧНИХ АГРОФОНІВ |
| title_short | ЕФЕКТИВНІСТЬ ПЕРЕДПОСІВНОЇ ІНОКУЛЯЦІЇ У ВИРОЩУВАННІ СІЛЬСЬКОГОСПОДАРСЬКИХ КУЛЬТУР ЗА РІЗНИХ ОРГАНІЧНИХ АГРОФОНІВ |
| title_sort | ефективність передпосівної інокуляції у вирощуванні сільськогосподарських культур за різних органічних агрофонів |
| topic | інокуляція мікробні препарати солома сидерат гній ВРХ генетично марковані штами бактерій |
| topic_facet | bean-rhizobial symbiosis nodular bacteria Mesorhizobium ciceri chickpea інокуляція мікробні препарати солома сидерат гній ВРХ генетично марковані штами бактерій |
| url | https://smic.in.ua/index.php/journal/article/view/339 |
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