АКТИВНІСТЬ ФЕРМЕНТНОГО ЦЕЛЮЛОЗОЛІТИЧНОГО КОМПЛЕКСУ ТА АНТАГОНІСТИЧНІ ВЛАСТИВОСТІ TRICHODERMA HARZIANUM 128

• Видавець: Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine
• Дата: 2021
• Автори: С. Б. , Дімова, С. М. , Деркач, В. В. , Волкогон
• Формат: Стаття
• Мова: Ukrainian
• Опубліковано: Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine 2021
• Теми: Trichoderma harzianum; cellulolytic enzymes; antagonistic activity; poultry manure; composting; compost; целюлозолітичні ферменти; антагоністична активність; пташиний послід; компостування; компости
• Онлайн доступ: https://smic.in.ua/index.php/journal/article/view/440

Objective. To study the activity of enzymes of the cellulolytic complex of the association of micromycetes Trichoderma harzianum 128 and their antagonistic activity; to study the possibility of the influence of fungal introduction to the composted substrate on the composting rate and quality of composts. Methods. Microbiological (for growing micromycetes in digest media, accounting the number of T. harzianum 128 and determining the antagonistic activity of the association), agrochemical (to determine the characteristics of composting chicken manure-based substrates and content of carbon and nitrogen in these substrates), biochemical (study of activity of cellulolytic enzymes), production inspection (on the basis of Agrofirma KOLOS LLC, Region of Kyiv). Results. The association of T. harzianum 128 micromycetes is characterized by high activity of exoglucanase, endoglucanase and β-glucosidase, which contributes to reduction in the composting time of organic matter upon the introduction of fungi to the chicken manure-based substrate. T. harzianum 128 has pronounced antifungal properties against pathogens of certain diseases of crops (Fusarium oxysporum, F. sulfurum, Nigrospora oryzae). Enrichment of composted substrates with the studied micromycetes provides their active development (up to 8,150 thous CFU/g of dry compost), accumulation of carbon and nitrogen in compost, which improves the quality of the final product. Conclusion. The association of T. harzianum 128 micromycetes is characterized by high activity of enzymes of the cellulolytic complex, has a pronounced antagonistic activity against pathogens of certain diseases of crops. The introduction of T. harzianum 128 to composted chicken manure-based substrates reduces the composting time and improves the quality of the final product, which can increase the productivity of agrocenoses by including biofertilizers in crop fertilization systems.