ВПЛИВ AZOTOBACTER VINELANDII ІМВ В-7076 НА РІСТ ПШЕНИЦІ ТА СИНТЕЗ ЕКЗОМЕТАБОЛІТІВ В УМОВАХ ГІДРОПОНІКИ
Objective. Determine the influence of Azotobacter vinelandii ІМV В-7076 on the in vitro synthesis of exometabolites by wheat plants. Methods. Experiments were carried out with the winter wheat of Shestopalivka variety, which seeds first underwent surface sterilized and then grown for 14 days under t...
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| author | Курдиш, І. К. Чоботарьов, А. Ю. Броварська, О. С. Пархоменко, Н. Й. Чоботарьова, В. В. |
| author_facet | Курдиш, І. К. Чоботарьов, А. Ю. Броварська, О. С. Пархоменко, Н. Й. Чоботарьова, В. В. |
| author_institution_txt_mv | [
{
"author": "І. К. Курдиш",
"institution": "Інститут мікробіології і вірусології ім. Д. К. Заболотного НАН України"
},
{
"author": "А. Ю. Чоботарьов",
"institution": "Інститут мікробіології і вірусології ім. Д. К. Заболотного НАН України"
},
{
"author": "О. С. Броварська",
"institution": "Інститут мікробіології і вірусології ім. Д. К. Заболотного НАН України"
},
{
"author": "Н. Й. Пархоменко",
"institution": "Інститут мікробіології і вірусології ім. Д. К. Заболотного НАН України"
},
{
"author": "В. В. Чоботарьова",
"institution": "Інститут мікробіології і вірусології ім. Д. К. Заболотного НАН України"
}
] |
| author_sort | Курдиш, І. К. |
| baseUrl_str | https://smic.in.ua/index.php/journal/oai |
| collection | OJS |
| datestamp_date | 2026-07-22T10:10:52Z |
| description | Objective. Determine the influence of Azotobacter vinelandii ІМV В-7076 on the in vitro synthesis of exometabolites by wheat plants. Methods. Experiments were carried out with the winter wheat of Shestopalivka variety, which seeds first underwent surface sterilized and then grown for 14 days under the conditions of hydroponics with A. vinelandii ІМV В-7076. Bacteria were grown in 700 mL E-flasks contained 100 mL of Ashby medium and incubated on the orbital shakers at 220 rpm at 28 °С during two days. The wheat plants were grown in 1.5 L cylindrical glass vessels of 115 mm in diameter and 160 mm height. Stainless steel meshes were placed in the vessels at a distance of 5 mm from the bottom. The vessels were sterilized, after which 75 mL of sterile Farreus medium was added. After seed germination, five sprouts were placed on stainless steel meshes in glasses. Azotobacter suspensions were added to the medium in three different dilutions (105, 106 and 107 cells/mL, respectively). The effect of bacteria on plant morphometric parameters, chlorophyll and carotenoid content in plant leaves, and the accumulation of carbohydrates, proteins and phenolic compounds in the medium with root exudates were determined. Results. It was found that growing wheat on a medium with A. vinelandii IMV B-7076 had a positive effect on plant growth, the content of photosynthetic pigments in leaves and the accumulation of proteins, phenols and carbohydrates in root exudates. The stimulating effect of Azotobacter increased with an increase in the content of cells in the medium. When growing plants on a medium with 107 cells/mL, the length of sprouts increased by 26.4 % compared to the control (without Azotobacter), and the weight of plants increased by 63.6 %. When growing wheat plants under such conditions, the content of proteins and carbohydrates in the medium with plant root exudates increased more than twice compared to the control, and phenolic compounds — by 79.8 %. Conclusion. A. vinelandii ІМV В-7076 has the positive effect on the growth and development of the wheat plants and synthesis of exometabolites. |
| doi_str_mv | 10.35868/1997-3004.40.37-45 |
| first_indexed | 2025-09-17T09:33:22Z |
| format | Article |
| fulltext |
37
ҐРУНТОВА МІКРОБІОЛОГІЯ
Сільськогосподарська мікробіологія. 2024. Вип. 40. С. 37–45.
ISSN 1997-3004
https://doi.org/10.35868/1997-3004.40.37-45
UDC 579.64:631.52
INFLUENCE OF AZOTOBACTER VINELANDII IMV B-7076
ON THE GROWTH OF WHEAT AND THE SYNTHESIS
OF EXOMETABOLITES IN HYDROPONIC CONDITIONS
I. K. Kurdish, A. Yu. Chobotarov, O. S. Brovarska,
N. Y. Parkhomenko, V. V. Chobotarova
Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154, Akademika Zabolotnoho Str., Kyiv, 03143, Ukraine; e-mail: ivan.kurdish2016@gmail.com
Objective. Determine the influence of Azotobacter vinelandii ІМV В-7076 on the in vitro syn-
thesis of exometabolites by wheat plants. Methods. Experiments were carried out with the winter
wheat of Shestopalivka variety, which seeds first underwent surface sterilized and then grown for
14 days under the conditions of hydroponics with A. vinelandii ІМV В-7076. Bacteria were grown
in 700 mL E-flasks contained 100 mL of Ashby medium and incubated on the orbital shakers at
220 rpm at 28 °С during two days. The wheat plants were grown in 1.5 L cylindrical glass vessels
of 115 mm in diameter and 160 mm height. Stainless steel meshes were placed in the vessels at a
distance of 5 mm from the bottom. The vessels were sterilized, after which 75 mL of sterile Farreus
medium was added. After seed germination, five sprouts were placed on stainless steel meshes in
glasses. Azotobacter suspensions were added to the medium in three different dilutions (105, 106
and 107 cells/mL, respectively). The effect of bacteria on plant morphometric parameters, chloro-
phyll and carotenoid content in plant leaves, and the accumulation of carbohydrates, proteins and
phenolic compounds in the medium with root exudates were determined. Results. It was found that
growing wheat on a medium with A. vinelandii IMV B-7076 had a positive effect on plant growth,
the content of photosynthetic pigments in leaves and the accumulation of proteins, phenols and car-
bohydrates in root exudates. The stimulating effect of Azotobacter increased with an increase in the
content of cells in the medium. When growing plants on a medium with 107 cells/mL, the length
of sprouts increased by 26.4 % compared to the control (without Azotobacter), and the weight of
plants increased by 63.6 %. When growing wheat plants under such conditions, the content of pro-
teins and carbohydrates in the medium with plant root exudates increased more than twice com-
pared to the control, and phenolic compounds — by 79.8 %. Conclusion. A. vinelandii ІМV В-7076
has the positive effect on the growth and development of the wheat plants and synthesis of exo-
metabolites.
Key words: Azotobacter vinelandii, wheat, phenols, carbohydrates, chlorophylls, carotenoids.
Introduction. The most critical factor for
life on Earth is vegetation, which ensures the
functioning of all the living organisms. In the
process of plant growth, about 40 % of the pho-
tosynthesis products are released into the basal
zone of the soil [1]. Due to the release of root
exudates around plant roots at a distance of up
to 4–6 mm from its surface, in the rhizosphere, a
unique microbial community is formed for each
plant species [2] the number of which signifi-
cantly exceeds similar indicators of the soil la-
yer, distant from the surface of the root [3].
Microorganisms of the rhizosphere play a
significant role in the growth, development, and
productivity of plants. They can improve the ni-
trogen and phosphorus nutrition of plants, the
availability of a number of mineral elements for
them, stimulate their growth and development
through the synthesis of phytohormones and
other biologically active compounds [4–11].
© I. K. Kurdish, A. Yu. Chobotarov, O. S. Brovarska, N. Y. Parkhomenko, V. V. Chobotarova, 2024
38
Microorganisms of the rhizosphere are able to
protect plants from the influence of a number of
negative environmental factors [12–14]. At the
same time, the influence of the microbiota of the
rhizosphere and its individual components on
the synthesis of root exudates has hardly been
investigated.
Analysis of recent studies and publica-
tions. An important direction of increasing the
positive influence of microorganisms on the
growth and productivity of plants is the use of
effective microbial preparations. For this pur-
pose, a significant number of such preparations
have been developed and are widely used in
crop production [6–8; 15].
Complex microbial preparations created on
the basis of two or more types of microorgan-
isms show the most noticeable positive effect on
the growth and productivity of plants [9; 14;
15]. Based on the interaction of nitrogen-fixing
bacteria Azotobacter vinelandii IMV B-7076
and phosphate-mobilizing bacteria Bacillus sub-
tilis IMV B-7023 with natural mineral particles,
we developed a complex bacterial preparation
Azogran, which significantly improves the
growth and productivity of a significant number
of plants [9]. The effect of the components of
this preparation on the synthesis of plant exo-
metabolites has not been studied. Considering
this, the objective of the work was to determine
the effect of Azotobacter vinelandii IMV B-7076
on the synthesis of exometabolites by wheat
plants.
Materials and methods. In the experi-
ments, Azotobacter vinelandii IMB B-7076 was
used, which was isolated in the Department of
Microbiological Processes on Hard Surfaces of
the D. K. Zabolotny Institute of Microbiology
and Virology, the NAS of Ukraine [16]. The
bacteria were grown in 700 mL Erlenmeyer
flasks containing 100 mL of Ashby medium
[17] and incubated on orbital shakers at 220 rpm
at 28 °C for two days. The population of azoto-
bacter in the suspension was determined by se-
rial dilution and plating onto agarized Ashby
medium, followed by cultivation and colony
counting.
The experiments were carried out on
Shestopalivka wheat provided by the National
Scientific Center “Institute of Agriculture” of
the NAS of Ukraine. Wheat plants were grown
in 1.5 liters cylindrical glass vessels with
a diameter of 115 mm, and a height of 160 mm.
Stainless steel meshes were placed in these ves-
sels at a distance of 5 mm from the bottom. The
vessels were sterilized at 160 °C, after which
75 mL of sterile Farreus medium of the follo-
wing composition per liter was added: CaCl2 —
0.1 g, MgSO4·7H2O — 0.12 g, KH2PO4 —
0.1 g, Na2HPO4·12H2O — 0.15 g, Fe citrate —
0.005 g, and trace amounts of Cu, Zn, B, Mn,
Mo, pH 6.5 [18].
Before application in the experiments, these
wheat seeds were sterilized for 7 minutes in
96 % ethanol and 50 % hydrogen peroxide with
the 1:1 ratio of these solutions. Then, the seeds
were thoroughly washed five times with sterile
distilled water and placed on the surface of po-
tato agar in Petri dishes. After the germination,
five sterile seeds were placed on stainless me-
shes in glasses. The study was conducted in four
variants. Bacteria were not added to the medium
of the 1st variant (control). In the following va-
riants, the concentration of azotobacter in the
medium was 105, 106, and 107 cells/mL, respec-
tively.
Plants were grown in a phytotron for 14
days at a temperature of 20 °C with 16 hours of
light. After that, the medium with the root exu-
date was sterilely drained for the subsequent de-
termination of the metabolite content; the length
of the plant shoots and roots and their weight
were determined.
To determine the content of chlorophylls a
and b and carotenoids in plants, 1 g fresh cru-
shed leaves were placed in a mortar and ground
with a small amount of magnesium carbonate;
10 mL of cooled 96 % ethanol was added under
thorough subsequent grinding for 2–3 minutes.
The resulting extract was carefully drained and
filtered through a membrane filter. The extrac-
tion was carried out several times until the fil-
trate stopped decolorizing. The resulting filtrate
was placed in a 25 mL volumetric flask, and the
required volume was made up with 96 % etha-
nol. The amount of green and yellow pig-
ments was determined spectrophotometrically
on SF-46 LOMO [19]. The absorption maxi-
mum of chlorophyll a was determined 665 nm,
that of chlorophyll b — at 649 nm, and that of
carotenoids — at 441 nm.
The concentration of chlorophyll a (Ca,
mg/L) and b (Cb, mg/L) was calculated using
the following formulas:
ISSN 1997-3004 Сільськогосподарська мікробіологія. 2024. Вип. 40.
39
Cа = 13.70 · А665 – 5.76 · А649,
Cb = 25.80 · A649 – 7.60 · A665,
where A665 is the optical density of the solution
at 665 nm;
A649 is the optical density of the solution at
649 nm.
The concentration of carotenoids (Cc,
mg/L) was calculated using the formula:
Сc = 4.695 · A441 – 0.268 (Сa + Сb),
where A441 is the optical density of the solution
at 441 nm;
(Сa + Сb) is the total content of chlorophyll
a and b in the solution, mg/L.
After determining the pigment concentra-
tions in the extract, the quantitative content of
the pigments (X, mg/mL) in the raw material
was calculated using the formula:
Х = V · C / m · 1000,
where: V is the volume of the alcoholic extract,
mL;
C is the concentration of chlorophyll in the
alcoholic solution, mg/L;
m is the weight of the raw material, g.
The protein content in cell-free supernatants
was determined according to the Bradford
method [20]. This method is based on the reac-
tion of Coomassie with arginine and hydropho-
bic amino acid residues.
The quantitative determination of carbohy-
drates was carried out using the colorimetric
method, which is based on the ability of both
free sugars and those composed of monosaccha-
ride residues of homo- and heteropolymers to
create a yellow-brown color during interaction
with phenol and sulfuric acid [21].
The total content of phenols was deter-
mined using the Folin-Ciocalteu reagent [22].
The reaction mixture contained the following
components: the test sample — 1 mL, Folin-
Ciocalteu reagent : H2O = 1 : 9 — 2.5 mL. After
mixing, the samples were placed in a dark place
for three minutes, then 2 mL of 3.5 % sodium
carbonate were added to each sample and placed
in a dark place again for two hours. The total
content of phenols was determined at 765 nm.
The calibration curve was constructed using gal-
lic acid. Statistical analysis of experimental data
was performed using Microsoft Excel 2010.
Results. It was established that during the
cultivation of wheat for 14 days in the Farre-
us medium, which contained 105 cells/mL of
A. vinelandii IMV B-7076, the stimulating ef-
fect of these bacteria on the growth and mass of
plants was insignificant. The length of the root
grew by only 1.8 %, the length of the stem —
by 8.8 %. At the same time, the average weight
of plants increased by 27.3 % compared to the
control (azotobacter was not used) (Table 1).
When wheat was grown in the medium with
106 cells/mL of these bacteria, the morphomet-
ric indicators of the plants increased more no-
ticeably. The indices for the length of the root
were 12.3 % higher, for the stem — 8.2 % hig-
her, and for the average plant weight — 27.3 %
higher than those in the control.
The most noticeable stimulatory effect was
observed for growing wheat in the medium with
the addition of 107 cells/mL. Under these condi-
tions, compared to the control, the root length
increased by 45.6 %, the stem length — by
Table 1. The influence of different contents of Azotobacter vinelandii IMV B-7076 on the
morphometric indicators of wheat plants when grown in the Farreus medium
Variants of research Average root length,
cm / %
Average stem length,
cm / %
Average mass of
plants, g / %
Medium without
A. vinelandii
5.7 ± 1.4
100.0
18.2 ± 1.8
100.0
0.11 ± 0.01
100.0
A. vinelandii,
(3.00 ± 0.06) · 105 cells/mL
5.8 ± 1.4
101.8
19.8 ± 1.9
108.8
0.14 ± 0.02
127.3
A. vinelandii,
(2.32 ± 0.02) · 106 cells/mL
6.4 ± 0.8
112.3
19.7 ± 0.9
108.2
0.14 ± 0.01
127.3
A. vinelandii,
(2.23 ± 0.03) · 107 cells/mL
8.3 ± 1.0
145.6
23.0 ± 1.8
126.4
0.18 ± 0.02
163.6
ISSN 1997-3004 Сільськогосподарська мікробіологія. 2024. Вип. 40.
40
26.4 %, and the average plant weight — by
63.6 % (Table 1).
It was established that after 14 days of
growing plants in a medium containing A. vinelan-
dii IMV B-7076 in the amount of 105 cells/mL,
the content of chlorophyll a in the leaves was
4 % higher than the value in the control (Tab-
le 2), and those for chlorophyll b and carote-
noids were lower than the indicators in the
control.
When plants were grown in the medium
with A. vinelandii IMV B-7076 in the amount of
106 cells/mL, the content of chlorophyll a in-
creased by 9 % compared to the control, and
that for chlorophyll b increased slightly. Gro-
wing wheat in a medium containing 107 cells/mL
increased the content of chlorophyll a and chlo-
rophyll b by 13 and 11 %, respectively. At the
same time, the concentration of carotenoids was
slightly lower than the values in the control.
The cultivation of wheat plants in Farreus
medium containing A. vinelandii IMV B-7076
cells increased the protein content in root exu-
dates (Fig. 1).
At the level of 105 and 106 cells/mL of
these bacteria in this medium, the protein con-
tent in the exudates increased by 68 % and
55 %, respectively, compared to the control.
When wheat was grown in the medium with the
addition of 107 cells/mL, the protein content in
root exudates increased more than twice com-
pared to the control.
The cultivation of wheat plants in the
Farreus medium, which contained the A. vine-
landii IMV B-7076 bacteria, influenced the ac-
cumulation of phenolic compounds in it (Fig. 2).
When these plants were grown in the control
variant (without bacteria), the content of these
compounds in the medium was 0.66 μg/mL.
Fig. 1. Protein content in the Farreus medi-
um in case of wheat cultivation for 14 days
and introduction of different amounts of
A. vinelandii bacteria: 1 — 105 cells/mL;
2 — 106 cells/mL; 3 — 107 cells/mL.
Fig. 2. The soil of phenolic compounds in
the Farreus medium for the cultivation of
wheat for 14 days and the introduction of
different amounts of A. vinelandii bacteria:
1 — 105 cells/mL; 2 — 106 cells/mL; 3 —
107 cells/mL.
When 105 or 106 cells/mL of these bacteria
were added to the medium, the content of these
compounds decreased by almost 20 % compa-
red to the control. At the same time, during
Table 2. The content of chlorophyll a and b and carotenoids in wheat plants when grown in
the Farreus medium with different contents of Azotobacter vinelandii IMV B-7076
Variants of research
Content of photosynthetic pigments, mg/g
chlorophyll a chlorophyll b carotenoids
Control (plants without azotobacter) 1.28 ± 0.18 0.80 ± 0.09 0.35 ± 0.04
Wheat plants + A. vinelandii,
(3.00 ± 0.06) · 105 cells/mL 1.33 ± 0.07 0.66 ± 0.08 0.34 ± 0.04
Wheat plants + A. vinelandii,
(2.32 ± 0.02) · 106 cells/mL 1.40 ± 0.02 0.82 ± 0.09 0.32 ± 0.04
Wheat plants + A. vinelandii,
(2.23 ± 0.03) · 107 cells/mL 1.45 ± 0.14 0.88 ± 0.09 0.34 ± 0.05
0,00
1,00
2,00
Control 1 2 3
To
ta
l p
ro
te
in
, µ
g/
m
L
Sample
0,00
1,00
2,00
Control 1 2 3
To
ta
l p
he
no
lic
c
on
te
nt
s,
µg
/m
L
Sample
ISSN 1997-3004 Сільськогосподарська мікробіологія. 2024. Вип. 40.
41
the cultivation of these plants in the medium
containing 107 cells/mL of azotobacter, the con-
tent of phenolic substances increased by 79.8 %
compared to the control (Fig. 2).
The cultivation of wheat plants in an envi-
ronment with the introduced A. vinelandii IMV
B-7076 had a noticeable effect on the accumula-
tion of carbohydrates in root exudates (Table 3).
While the carbohydrate content of dry exudate
in the control was 26.8 μg/mg, during the cul-
tivation of plants in a medium containing
105 cells/mL the amount of carbohydrates in-
creased to 41.8 μg/mg of dry exudate mass,
and with azotobacter in the amount of 106 and
107 cells/mL, the concentration of carbohydrates
increased to 58.7 and 65.7 μg/mg.
Table 3. Total carbohydrate content in
dried preparations of exudates of wheat grown
with Azotobacter vinelandii IMV B-7076
Variants of research
Carbohydrate
content in
exudates, μg/mg
Control (plants without
azotobacter) 26.80 ± 1.30
Wheat plants + A. vinelandii,
(3.00 ± 0.06) · 105 cells/mL 41.80 ± 2.10
Wheat plants + A. vinelandii,
(2.32 ± 0.02) · 106 cells/mL 58.73 ± 2.55
Wheat plants + A. vinelandii,
(2.23 ± 0.03) · 107 cells/mL 65.77 ± 3.29
Thus, the cultivation of Shestopalivka
wheat plants in hydroponic conditions with
A. vinelandii IMV B-7076 in the medium
caused a noticeable stimulating effect on the
morphometric indicators of the plants, the con-
tent of photosynthetic pigments in them, the ac-
cumulation of proteins, phenolic substances, and
carbohydrates in root exudates.
Discussion. The intensive use of chemical
fertilizers, pesticides, and other chemical com-
pounds in crop production harms the environ-
ment, reduces the quality of the obtained plant
products, and can have a negative impact on
people’s health. With this in mind, in the last
decade, considerable attention has been paid to
the biologization of crop production [23]. An
essential component of this approach is the use
of microbial preparations in crop production,
which can improve the growth, development,
and productivity of plants without harming the
environment. One of them is the complex bacte-
rial preparation Azogran. This drug improves
the growth and productivity of plants signifi-
cantly [9; 10]. Its component is Azotobacter
vinelandii IMV B-7076.
The results of our research show that under
hydroponic cultivation of Shestopalivka wheat
plants, the effect of this strain on the growth ac-
tivity of plants depends on the concentration of
these bacteria in the cultivation medium. After
14 days of growing wheat with 107 cells/mL of
A. vinelandii IMV B-7076 in the medium, the
length of the plant stems was 26.4 % longer
compared to the control. The obtained data con-
firm the results of our previous research on the
stimulating effect of the Azogran preparation on
the morphometric indicators of wheat plants of
other varieties [24].
A significant role in the growth and produc-
tivity of plants belongs to photosynthetic pig-
ments, chlorophyll a and b, and carotenoids,
which, in the process of functioning in the plant
leaves, transform the energy of solar radiation
into the energy of chemical bonds, which is the
basis for the functioning of living organisms.
We have shown that when growing wheat plants
in the medium with A. vinelandii IMV B-7076,
the content of chlorophyll a increases and is
higher than the index of chlorophyll b and ca-
rotenoids.
It is known that photosynthetic pigments
are extremely sensitive to the physiological
state of plants. Even a short-term stress leads to
a change in the total content of these pigments
[25]. In plants, affected by phytopathogens, the
content of chlorophyll b increases significantly
[26], while the content of carotenoids can de-
crease considerably [27].
The results of our research demonstrate
a positive effect of the preparation Azogran on
the functioning of the photosynthetic system of
wheat plants, on their development and accumu-
lation of phenols, proteins, and carbohydrates in
the root exudates of these plants.
Conclusions. The results of our study
demonstrated a positive effect of A. vinelandii
IMV B-7076 bacteria on the growth and deve-
lopment of wheat under conditions of hydropo-
nic cultivation and the accumulation of biolo-
gically active substances in the root exudates
of these plants. It was established that the high-
est stimulatory effect of these bacteria was
ISSN 1997-3004 Сільськогосподарська мікробіологія. 2024. Вип. 40.
42
observed when growing plants in the medium
with the addition of 107 cells/mL of A. vinelan-
dii IMV B-7076. Under these conditions, the
length of the stem was 26.4 % greater than the
indices in the control, and the weight of the
plants increased by 63.6 %. The content of chlo-
rophyll a and b increased by 13 % and 11 %, re-
spectively. When growing wheat in the medium
containing such amounts of azotobacter, the
content of proteins, phenolic compounds, and
carbohydrates in plant root exudates increased
significantly. The obtained results demonstrated
a significant stimulating effect of A. vinelandii
IMV B-7076 on the growth and development of
wheat plants and the synthesis of exometabo-
lites by these plants.
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of Plant Physiology, 15(4), 399–403.
Received 24.07.2024
https://doi.org/10.35868/1997-3004.40.37-45
УДК 579.64:631.52
ВПЛИВ AZOTOBACTER VINELANDII ІМВ В-7076 НА РІСТ ПШЕНИЦІ
ТА СИНТЕЗ ЕКЗОМЕТАБОЛІТІВ В УМОВАХ ГІДРОПОНІКИ
І. К. Курдиш, А. Ю. Чоботарьов, О. С. Броварська,
Н. Й. Пархоменко, В. В. Чоботарьова
Інститут мікробіології і вірусології ім. Д. К. Заболотного НАН України
e-mail: ivan.kurdish2016@gmail.com
Мета. Визначити вплив бактерії Azotobacter vinelandii ІМВ В-7076 на синтез екзоме-
таболітів рослинами пшениці в умовах in vitro. Методи. Досліди проводили з пшеницею ози-
мою сорту Шестопалівка, насіння якої після поверхневої стерилізації вирощували впродовж
14 діб в умовах гідропоніки з бактерією A. vinelandii ІМВ В-7076. Бактерії вирощували в кол-
бах Ерленмейєра на 700 мл, що містили 100 мл середовища Ешбі, та інкубували на орбіталь-
них шейкерах при 220 об./хв. за 28 °С протягом двох діб. Рослини пшениці вирощували в ци-
ліндричних скляних посудинах об’ємом 1,5 л, діаметром 115 мм, висотою 160 мм. Сітки
ISSN 1997-3004 Сільськогосподарська мікробіологія. 2024. Вип. 40.
44
з нержавіючої сталі поміщали в посудини на відстані 5 мм від дна. Посудини стерилізували,
після чого додавали 75 мл стерильного середовища Фарреуса. Після пророщування насіння
п’ять паростків поміщали на нержавіючі сітки в склянки. До середовища додавали суспензії
азотобактера в трьох різних розведеннях (105, 106 і 107 клітин/мл відповідно). Визначали
вплив бактерій на морфометричні показники рослин, вміст хлорофілів і каротиноїдів у лис-
тках рослин, накопичення вуглеводів, білків і фенольних сполук у середовищі з кореневими ек-
судатами. Результати. Встановлено, що вирощування пшениці на середовищі з бактерією
A. vinelandii ІМВ В-7076 позитивно впливало на ріст рослин, вміст фотосинтетичних піг-
ментів у листках та накопичення білків, фенолів і вуглеводів у кореневих ексудатах. Стиму-
лювальна дія азотобактера зростала зі збільшенням вмісту клітин у середовищі. При виро-
щуванні рослин на середовищі з 107 кл./мл довжина паростків збільшилася на 26,4 % проти
контролю (без азотобактера), а маса рослин підвищилася на 63,6 %. При вирощуванні рос-
лин пшениці за таких умов уміст білків і вуглеводів у середовищі з кореневими ексудатами
рослин зріс більш ніж удвічі проти контролю, а фенольних сполук — на 79,8 %. Виснов-
ки. A. vinelandii ІМВ В-7076 позитивно впливає на ріст і розвиток рослин пшениці та синтез
ними екзометаболітів.
Ключові слова: Azotobacter vinelandii, пшениця, феноли, вуглеводи, хлорофіли, кароти-
ноїди.
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Отримано 24.07.2024
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| id | oai:ojs2.smic.in.ua:article-530 |
| institution | Agriciltural microbiology |
| keywords_txt_mv | keywords |
| language | English |
| last_indexed | 2026-07-23T01:16:14Z |
| publishDate | 2024 |
| publisher | Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine |
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| resource_txt_mv | smicinua/e0/e290ad525530782d3e93f99c086b8ee0.pdf |
| spelling | oai:ojs2.smic.in.ua:article-5302026-07-22T10:10:52Z INFLUENCE OF AZOTOBACTER VINELANDII IMV B-7076 ON THE GROWTH OF WHEAT AND THE SYNTHESIS OF EXOMETABOLITES IN HYDROPONIC CONDITIONS ВПЛИВ AZOTOBACTER VINELANDII ІМВ В-7076 НА РІСТ ПШЕНИЦІ ТА СИНТЕЗ ЕКЗОМЕТАБОЛІТІВ В УМОВАХ ГІДРОПОНІКИ Курдиш, І. К. Чоботарьов, А. Ю. Броварська, О. С. Пархоменко, Н. Й. Чоботарьова, В. В. Azotobacter vinelandii, wheat, phenols, carbohydrates, chlorophylls, carotenoids Azotobacter vinelandii, пшениця, феноли, вуглеводи, хлорофіли, каротиноїди Objective. Determine the influence of Azotobacter vinelandii ІМV В-7076 on the in vitro synthesis of exometabolites by wheat plants. Methods. Experiments were carried out with the winter wheat of Shestopalivka variety, which seeds first underwent surface sterilized and then grown for 14 days under the conditions of hydroponics with A. vinelandii ІМV В-7076. Bacteria were grown in 700 mL E-flasks contained 100 mL of Ashby medium and incubated on the orbital shakers at 220 rpm at 28 °С during two days. The wheat plants were grown in 1.5 L cylindrical glass vessels of 115 mm in diameter and 160 mm height. Stainless steel meshes were placed in the vessels at a distance of 5 mm from the bottom. The vessels were sterilized, after which 75 mL of sterile Farreus medium was added. After seed germination, five sprouts were placed on stainless steel meshes in glasses. Azotobacter suspensions were added to the medium in three different dilutions (105, 106 and 107 cells/mL, respectively). The effect of bacteria on plant morphometric parameters, chlorophyll and carotenoid content in plant leaves, and the accumulation of carbohydrates, proteins and phenolic compounds in the medium with root exudates were determined. Results. It was found that growing wheat on a medium with A. vinelandii IMV B-7076 had a positive effect on plant growth, the content of photosynthetic pigments in leaves and the accumulation of proteins, phenols and carbohydrates in root exudates. The stimulating effect of Azotobacter increased with an increase in the content of cells in the medium. When growing plants on a medium with 107 cells/mL, the length of sprouts increased by 26.4 % compared to the control (without Azotobacter), and the weight of plants increased by 63.6 %. When growing wheat plants under such conditions, the content of proteins and carbohydrates in the medium with plant root exudates increased more than twice compared to the control, and phenolic compounds — by 79.8 %. Conclusion. A. vinelandii ІМV В-7076 has the positive effect on the growth and development of the wheat plants and synthesis of exometabolites. Мета. Визначити вплив бактерії Azotobacter vinelandii ІМВ В-7076 на синтез екзометаболітів рослинами пшениці в умовах in vitro. Методи. Досліди проводили з пшеницею озимою сорту Шестопалівка, насіння якої після поверхневої стерилізації вирощували впродовж 14 діб в умовах гідропоніки з бактерією A. vinelandii ІМВ В-7076. Бактерії вирощували в колбах Ерленмейєра на 700 мл, що містили 100 мл середовища Ешбі, та інкубували на орбітальних шейкерах при 220 об./хв. за 28 °С протягом двох діб. Рослини пшениці вирощували в циліндричних скляних посудинах об’ємом 1,5 л, діаметром 115 мм, висотою 160 мм. Сітки з нержавіючої сталі поміщали в посудини на відстані 5 мм від дна. Посудини стерилізували, після чого додавали 75 мл стерильного середовища Фарреуса. Після пророщування насіння п’ять паростків поміщали на нержавіючі сітки в склянки. До середовища додавали суспензії азотобактера в трьох різних розведеннях (105, 106 і 107 клітин/мл відповідно). Визначали вплив бактерій на морфометричні показники рослин, вміст хлорофілів і каротиноїдів у листках рослин, накопичення вуглеводів, білків і фенольних сполук у середовищі з кореневими ексудатами. Результати. Встановлено, що вирощування пшениці на середовищі з бактерією A. vinelandii ІМВ В-7076 позитивно впливало на ріст рослин, вміст фотосинтетичних пігментів у листках та накопичення білків, фенолів і вуглеводів у кореневих ексудатах. Стимулювальна дія азотобактера зростала зі збільшенням вмісту клітин у середовищі. При вирощуванні рослин на середовищі з 107 кл./мл довжина паростків збільшилася на 26,4 % проти контролю (без азотобактера), а маса рослин підвищилася на 63,6 %. При вирощуванні рослин пшениці за таких умов уміст білків і вуглеводів у середовищі з кореневими ексудатами рослин зріс більш ніж удвічі проти контролю, а фенольних сполук — на 79,8 %. Висновки. A. vinelandii ІМВ В-7076 позитивно впливає на ріст і розвиток рослин пшениці та синтез ними екзометаболітів. Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine 2024-10-23 Article Article Рецензована Стаття application/pdf https://smic.in.ua/index.php/journal/article/view/530 10.35868/1997-3004.40.37-45 Agricultural microbiology; Vol. 40 (2024): Agriciltural microbiology; 37-45 Сільськогосподарська мікробіологія; Том 40 (2024): Сільськогосподарська мікробіологія; 37-45 1997-3004 10.35868/1997-3004.40 en https://smic.in.ua/index.php/journal/article/view/530/599 Авторське право (c) 2024 I. K. Kurdish, A. Yu. Chobotarov, O. S. Brovarska, N. Y. Parkhomenko, V. V. Chobotarova https://creativecommons.org/licenses/by/4.0 |
| spellingShingle | Azotobacter vinelandii пшениця феноли вуглеводи хлорофіли каротиноїди Курдиш, І. К. Чоботарьов, А. Ю. Броварська, О. С. Пархоменко, Н. Й. Чоботарьова, В. В. ВПЛИВ AZOTOBACTER VINELANDII ІМВ В-7076 НА РІСТ ПШЕНИЦІ ТА СИНТЕЗ ЕКЗОМЕТАБОЛІТІВ В УМОВАХ ГІДРОПОНІКИ |
| title | ВПЛИВ AZOTOBACTER VINELANDII ІМВ В-7076 НА РІСТ ПШЕНИЦІ ТА СИНТЕЗ ЕКЗОМЕТАБОЛІТІВ В УМОВАХ ГІДРОПОНІКИ |
| title_alt | INFLUENCE OF AZOTOBACTER VINELANDII IMV B-7076 ON THE GROWTH OF WHEAT AND THE SYNTHESIS OF EXOMETABOLITES IN HYDROPONIC CONDITIONS |
| title_full | ВПЛИВ AZOTOBACTER VINELANDII ІМВ В-7076 НА РІСТ ПШЕНИЦІ ТА СИНТЕЗ ЕКЗОМЕТАБОЛІТІВ В УМОВАХ ГІДРОПОНІКИ |
| title_fullStr | ВПЛИВ AZOTOBACTER VINELANDII ІМВ В-7076 НА РІСТ ПШЕНИЦІ ТА СИНТЕЗ ЕКЗОМЕТАБОЛІТІВ В УМОВАХ ГІДРОПОНІКИ |
| title_full_unstemmed | ВПЛИВ AZOTOBACTER VINELANDII ІМВ В-7076 НА РІСТ ПШЕНИЦІ ТА СИНТЕЗ ЕКЗОМЕТАБОЛІТІВ В УМОВАХ ГІДРОПОНІКИ |
| title_short | ВПЛИВ AZOTOBACTER VINELANDII ІМВ В-7076 НА РІСТ ПШЕНИЦІ ТА СИНТЕЗ ЕКЗОМЕТАБОЛІТІВ В УМОВАХ ГІДРОПОНІКИ |
| title_sort | вплив azotobacter vinelandii імв в-7076 на ріст пшениці та синтез екзометаболітів в умовах гідропоніки |
| topic | Azotobacter vinelandii пшениця феноли вуглеводи хлорофіли каротиноїди |
| topic_facet | Azotobacter vinelandii wheat phenols carbohydrates chlorophylls carotenoids Azotobacter vinelandii пшениця феноли вуглеводи хлорофіли каротиноїди |
| url | https://smic.in.ua/index.php/journal/article/view/530 |
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