ЧУТЛИВІСТЬ РИЗОБАКТЕРІЙ РІЗНИХ ВИДІВ ДО ВПЛИВУ ҐРУНТОВИХ ГЕРБІЦИДІВ В УМОВАХ IN VITRO

• Datum: 2025
• Hauptverfasser: Коць, С. Я., Хоменко, В. Д., Кукол, К. П., Пухтаєвич, П. П.
• Format: Artikel
• Sprache: Englisch
• Veröffentlicht: Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine 2025
• Schlagworte: Bradyrhizobium japonicum; Bradyrhizobium elkanii; Azospirillum brasilense; Pseudomonas fluorescens; Azotobacter chroococcum; ґрунтовий гербіцид; чутливість; зона затримки росту
• Online Zugang: https://smic.in.ua/index.php/journal/article/view/544
• Назва журналу: Agriciltural microbiology

Objective. To investigate the sensitivity of Bradyrhizobium japonicum, Bradyrhizobium elkanii, Azospirillum brasilense, Pseudomonas fluorescens, and Azotobacter chroococcum to the effects of soil-applied herbicides under in vitro conditions. Methods. Microbiological and statistical. The sensitivity of rhizobacteria of different species to herbicidal preparations was studied using the well diffusion method on agarized nutrient medium plates. Results. As a result of the laboratory experiments series, it was established that the rhizobial strains Bradyrhizobium japonicum 634b, РС07, РС08, B20, T66, T21-2, B78 and Bradyrhizobium elkanii SAF18 were not sensitive to average recommended rate herbicide formulations containing 312.5 g/l S-metolachlor + 187 g/l terbuthylazine, 900 g/l acetochlor and 960 g/l S-metolachlor. A low level of sensitivity was observed in certain rhizobial strains to a herbicide formulation containing 500 g/l prometryn. Under the influence of the specified active ingredient, the growth inhibition zones on bacterial lawns of the analytically selected strains B. elkanii SAF18 and B. japonicum РС07 measured 5.0 mm and 11.0 mm, respectively. Exposure to prometryn at the tested concentration resulted in the formation of growth inhibition zones measuring 8.0 mm and 6.5 mm on bacterial lawns of Tn5 mutants of B. japonicum strains B20 and B78, respectively. As a result of examining the growth characteristics of A. brasilense 410, A. chroococcum T79, and P. fluorescens strains 33 and 267 under the influence of soil-applied herbicides, no growth inhibition zones were observed on the bacterial lawns of any of the tested strains. The formation and appearance of bacterial colonies were characteristic of each of the species studied. Conclusions. It was found that, under in vitro conditions, the chemical plant protection products used in the study did not exhibit bactericidal activity against rhizobacteria of various species under the conditions of applying the manufacturer’s recommended average rate. In vegetation and field experiments, it is advisable to determine the effect of herbicidal compounds on the realization of the symbiotic potential of rhizobia. It is also important to assess how these substances influence the plant growth-promoting properties of Pseudomonas, Azotobacter and Azospirillum strains. Such studies will contribute to resolving the issue of compatibility between the application of chemical plant protection products and complex bacterial inoculation.