ОСОБЛИВОСТІ ПІДТРИМАННЯ ШТАМІВ ТА ІЗОЛЯТІВ ВІРУСУ СКРУЧУВАННЯ ЛИСТЯ КАРТОПЛІ В КОЛЕКЦІЇ ФІТОПАТОГЕННИХ ВІРУСІВ КАРТОПЛІ

• Дата: 2026
• Автори: Решотько, Л. М., Волкова, І. В.
• Формат: Стаття
• Мова: Українська
• Опубліковано: Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine 2026
• Теми: віруси картоплі; колекція; підтримання штамів; ВСЛК; Potato leafroll virus
• Онлайн доступ: https://smic.in.ua/index.php/journal/article/view/561
• Назва журналу: Agriciltural microbiology

Objective. To analyze the peculiarities of maintaining strains and isolates of the potato leafroll virus (PLRV) in the collection of potato phytopathogenic viruses. Methods. Collection samples of PLRV are stored and studied using visual diagnosis, indicator plants, electron microscopy, immunological, and molecular genetic methods. Results. To maintain PLRV strains and isolates in the collection of potato phytopathogenic viruses (the Collection), a duplicate management system for infected plant samples is used. Firstly, potato plants are grown in open-field conditions, despite the risks of secondary infection, which allows for the reproduction of the natural rhythm of vegetation and tuber formation and contributes to a significant increase in material without the risk of its loss. Secondly, a method for the long-term cultivation of phytopathogenic viruses in crop plants in vitro under sterile conditions has been developed and implemented into routine practice, which has eliminated the risk of accumulation of fungal and bacterial pathogens and reinfection with other viruses. Microbulbs formed in test tubes are periodically planted in growing containers under controlled conditions and in open ground, gradually replacing the tuber part of the collection. Thus, the Collection simultaneously contains tuber material from open ground, sterile in vitro culture plants, and microtubers, as well as material obtained from microtubers under sterile conditions. In 2009, the introduction, maintenance, and preservation of PLRV strains and isolates in mammalian cell culture were added to the described technology for the Collection’s operation. Observations made during the maintenance, storage, and use of collection samples are reflected in the technical specifications of each strain and isolate. Currently, the Collection includes 2 strains in tubers and in potato plants during the growing season, and 3 strains in mammalian cell culture. Conclusions. Since a distinctive feature of potato viruses is their near-total absence in seeds, strains and isolates can only be preserved in green plants or in tubers. Under these conditions, the collection can be maintained through dual virus storage: in the form of virus-infected potato plants in summer and tubers in winter, and in the form of sterile test-tube-cultured infected potato plants throughout the year. Such a “living” collection requires constant updating and monitoring. For the VSLK, there is also the possibility of maintaining samples using an alternative method — in mammalian cell culture.