Вивчення люмінесценції замінника гуаніну при низьких температурах для реєстрації біополімерів

• Main Authors: Kudrya, V. Yu., Yashchuk, V. M., Naumenko, A. P., Mely, Y., Gumenyuk, Ya. O.
• Format: Article
• Language: English
• Published: Publishing house "Academperiodika" 2020
• Subjects: emissive guanine substitute; fluorescence; phosphorescence; energy transfer by excitations; -
• Online Access: https://ujp.bitp.kiev.ua/index.php/ujp/article/view/2019626

The optical absorption at 300 K and the fluorescence and phosphorescence at 78 K of the emissive guanine substitute, deoxythienoguanosine, (dthG) were investigated in aqueous and TRIS-HCl-buffer solutions. Two optical absorption and fluorescence centers at room temperature were attributed to two keto-enol tautomers of dthG, which confirms previously obtained results. In contrast to room temperature, only one emission band was observed at 78 K in fluorescence spectra that was close to the long-wave fluorescence band at room temperature and could be associated with the tautomer with long-wave absorption. This phenomenon can be explained by the energy transfer by excitations in a frozen solution between two types of the optical centers mentioned above. The similar conclusion is drawn for the phosphorescence: only one tautomer phosphorescence band is observed. The spectral positions of this band maximum are essentially different for aqueous and buffer solutions (∼50 nm).