Вивчення люмінесценції замінника гуаніну при низьких температурах для реєстрації біополімерів
The optical absorption at 300 K and the fluorescence and phosphorescence at 78 K of the emissive guanine substitute, deoxythienoguanosine, (dthG) were investigated in aqueous and TRIS-HCl-buffer solutions. Two optical absorption and fluorescence centers at room temperature were attributed to two ket...
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| Datum: | 2020 |
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| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | English |
| Veröffentlicht: |
Publishing house "Academperiodika"
2020
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| Online Zugang: | https://ujp.bitp.kiev.ua/index.php/ujp/article/view/2019626 |
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| Назва журналу: | Ukrainian Journal of Physics |
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Ukrainian Journal of Physics| Zusammenfassung: | The optical absorption at 300 K and the fluorescence and phosphorescence at 78 K of the emissive guanine substitute, deoxythienoguanosine, (dthG) were investigated in aqueous and TRIS-HCl-buffer solutions. Two optical absorption and fluorescence centers at room temperature were attributed to two keto-enol tautomers of dthG, which confirms previously obtained results. In contrast to room temperature, only one emission band was observed at 78 K in fluorescence spectra that was close to the long-wave fluorescence band at room temperature and could be associated with the tautomer with long-wave absorption. This phenomenon can be explained by the energy transfer by excitations in a frozen solution between two types of the optical centers mentioned above. The similar conclusion is drawn for the phosphorescence: only one tautomer phosphorescence band is observed. The spectral positions of this band maximum are essentially different for aqueous and buffer solutions (∼50 nm). |
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