Флуоресцентний моніторинг вивільнення іонів міді з поверхні функціональних матеріалів з використанням біосумісних хемосенсорних молекул

• Опубліковано в: Хімія, фізика та технологія поверхні
• Дата: 2026
• Автори: Eremenko, A.M., Petryk , I.S., Smirnova, N.P., Naumenko, A.P.
• Формат: Стаття
• Опубліковано: Chuiko Institute of Surface Chemistry National Academy of Sciences of Ukraine 2026
• Теми: флуоресценція; акридиновий помаранчевий; іони міді; додецилсульфат натрію; функція Штерна-Фольмера
• Онлайн доступ: https://www.cpts.com.ua/index.php/cpts/article/view/898
• Назва журналу: Chemistry, Physics and Technology of Surface

The use of organic dyes or biomolecules that fluoresce under the action of UV or visible light – fluorescent chemosensors – allows for high-precision quantitative assessment of the level of metal ions in water or biological fluids in living cells by quenching their fluorescence when interacting with transition metal ions, in particular Cu2+. The fluorescence of acridine orange (AO) when interacting with a metal ion is quenched in proportion to the number of interacting ions. according to the Stern-Volmer function. The dependence of the specific amount of copper ions released from the tissue surface into the aqueous solution of AO and sodium dodecyl sulfate (SDS), calculated from the AO fluorescence decay spectra, is nonlinear. Its shape indicates that both mechanisms of fluorescence quenching are present in the system: dynamic and static. It is shown that in the presence of SDS micelles at pH ~ 8, the efficiency of fluorescence quenching is significantly increased and no re-adsorption of copper ions on the surface of the fabric is observed, which allows such a determination to be carried out in situ, directly in biological environments. This method makes it possible to assess quantitatively the dynamics of desorption of metal ions that have bactericidal properties over time and determine the optimal time for using wound dressings (powders, hydrogels, textiles, films, and other systems with action based on the release of ions of copper and other heavy metals).