Features of embryonic development of parasitic nematodes of the species Trichuris sylvilagi (Nematoda, Trichuridae)

In the present study we established in vitro the terms and stages of exogenous development of nematodes of the species Trichuris sylvilagi Tiner, 1950 isolated from Lepus europaeus, and the degree of the nematode viability at different temperatures. It was found that the most favorable temperature f...

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Дата:2021
Автори: Yevstafieva, V., Melnychuk, V. , Nagorna, L. , Stybel, V. , Gutyj, B. , Yatsenko, I. , Petrenko, M. , Nikiforova, O. , Filonenko, S. , Savenkova, O. , Tahiltseva, Y. 
Формат: Стаття
Мова:English
Опубліковано: Publishing House "Akademperiodyka" of the National Academy of Sciences of Ukraine 2021
Онлайн доступ:https://ojs.akademperiodyka.org.ua/index.php/Zoodiversity/article/view/203
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Назва журналу:Journal Zoodiversity

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Journal Zoodiversity
id zoodiv-article-203
record_format ojs
spelling zoodiv-article-2032021-08-25T09:38:35Z Features of embryonic development of parasitic nematodes of the species Trichuris sylvilagi (Nematoda, Trichuridae) Yevstafieva, V. Melnychuk, V.  Nagorna, L.  Stybel, V.  Gutyj, B.  Yatsenko, I.  Petrenko, M.  Nikiforova, O.  Filonenko, S.  Savenkova, O.  Tahiltseva, Y.  Trichuris sylvilagi nematode eggs exogenous development temperature regime viability In the present study we established in vitro the terms and stages of exogenous development of nematodes of the species Trichuris sylvilagi Tiner, 1950 isolated from Lepus europaeus, and the degree of the nematode viability at different temperatures. It was found that the most favorable temperature for embryonic development of parasitic helminths was 30°C, with the formation of 88.67 % of viable eggs with a motile larva in 20 days in laboratory conditions, and a lethality rate of 11.33 % of eggs. With a decrease in temperature to 20°C, the embryogenesis occurred in 32 days. The viability of eggs, cultured at that temperature regime, decreased: 68.0 % of eggs reached larval stage, and 32.0 % of eggs died. At 10°C eggs did not develop. At the 32nd day of cultivation, 27.33 % of eggs remained at the zygote stage, 52.0 % at the stage of blastomere cleavage and formation, and 20.67 % died. Embryogenesis was characterized by metric changes in egg parameters. At optimal temperature the growth and development of eggs was accompanied by an increase in the egg width and plugs with a simultaneous decrease in the length of eggs and plugs, as well as thinning of the shell. Publishing House "Akademperiodyka" of the National Academy of Sciences of Ukraine 2021-07-01 Article Article application/pdf https://ojs.akademperiodyka.org.ua/index.php/Zoodiversity/article/view/203 Zoodiversity; Vol. 55 No. 4 (2021): Zoodiversity Zoodiversity (Vestnik Zoologii); Том 55 № 4 (2021): Zoodiversity 2707-7268 2707-725X 10.15407/zoo2021.04 en https://ojs.akademperiodyka.org.ua/index.php/Zoodiversity/article/view/203/114 Copyright (c) 2021 Zoodiversity
institution Journal Zoodiversity
baseUrl_str
datestamp_date 2021-08-25T09:38:35Z
collection OJS
language English
topic_facet Trichuris sylvilagi
nematode eggs
exogenous development
temperature regime
viability
format Article
author Yevstafieva, V.
Melnychuk, V. 
Nagorna, L. 
Stybel, V. 
Gutyj, B. 
Yatsenko, I. 
Petrenko, M. 
Nikiforova, O. 
Filonenko, S. 
Savenkova, O. 
Tahiltseva, Y. 
spellingShingle Yevstafieva, V.
Melnychuk, V. 
Nagorna, L. 
Stybel, V. 
Gutyj, B. 
Yatsenko, I. 
Petrenko, M. 
Nikiforova, O. 
Filonenko, S. 
Savenkova, O. 
Tahiltseva, Y. 
Features of embryonic development of parasitic nematodes of the species Trichuris sylvilagi (Nematoda, Trichuridae)
author_facet Yevstafieva, V.
Melnychuk, V. 
Nagorna, L. 
Stybel, V. 
Gutyj, B. 
Yatsenko, I. 
Petrenko, M. 
Nikiforova, O. 
Filonenko, S. 
Savenkova, O. 
Tahiltseva, Y. 
author_sort Yevstafieva, V.
title Features of embryonic development of parasitic nematodes of the species Trichuris sylvilagi (Nematoda, Trichuridae)
title_short Features of embryonic development of parasitic nematodes of the species Trichuris sylvilagi (Nematoda, Trichuridae)
title_full Features of embryonic development of parasitic nematodes of the species Trichuris sylvilagi (Nematoda, Trichuridae)
title_fullStr Features of embryonic development of parasitic nematodes of the species Trichuris sylvilagi (Nematoda, Trichuridae)
title_full_unstemmed Features of embryonic development of parasitic nematodes of the species Trichuris sylvilagi (Nematoda, Trichuridae)
title_sort features of embryonic development of parasitic nematodes of the species trichuris sylvilagi (nematoda, trichuridae)
description In the present study we established in vitro the terms and stages of exogenous development of nematodes of the species Trichuris sylvilagi Tiner, 1950 isolated from Lepus europaeus, and the degree of the nematode viability at different temperatures. It was found that the most favorable temperature for embryonic development of parasitic helminths was 30°C, with the formation of 88.67 % of viable eggs with a motile larva in 20 days in laboratory conditions, and a lethality rate of 11.33 % of eggs. With a decrease in temperature to 20°C, the embryogenesis occurred in 32 days. The viability of eggs, cultured at that temperature regime, decreased: 68.0 % of eggs reached larval stage, and 32.0 % of eggs died. At 10°C eggs did not develop. At the 32nd day of cultivation, 27.33 % of eggs remained at the zygote stage, 52.0 % at the stage of blastomere cleavage and formation, and 20.67 % died. Embryogenesis was characterized by metric changes in egg parameters. At optimal temperature the growth and development of eggs was accompanied by an increase in the egg width and plugs with a simultaneous decrease in the length of eggs and plugs, as well as thinning of the shell.
publisher Publishing House "Akademperiodyka" of the National Academy of Sciences of Ukraine
publishDate 2021
url https://ojs.akademperiodyka.org.ua/index.php/Zoodiversity/article/view/203
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