Molecular diagnostics based on surface plasmon angular spectroscopy
With the purpose to reliably detect specific antibodies against the Epstein–Barr virus (EBV) and cardiovascular system molecular markers (myoglobin and fibrinogen) levels in the buffer solutions, human blood plasma and serum, it has been analyzed some techniques for protein immobilization from immun...
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| Дата: | 2020 |
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Інститут проблем реєстрації інформації НАН України
2020
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drspiprikievua-article-2188242020-12-23T11:03:30Z Molecular diagnostics based on surface plasmon angular spectroscopy Молекулярна діагностика на основі кутової спектроскопії поверхневих плазмонів Kostyukevych, K. V. Khrystosenko, R. V. Zagorodnya, S. D. Kostyukevych, S. O. Koptiukh, A. A. Kryuchyn, A. A. Oleksenko, P. F. поверхневий плазмонний резонанс антитіла міоглобін фібриноген віруси буферні розчини плазма та сироватка крові surface plasmon resonance antibodies viruses myoglobin fibrinogen buffer solutions blood plasma and serum With the purpose to reliably detect specific antibodies against the Epstein–Barr virus (EBV) and cardiovascular system molecular markers (myoglobin and fibrinogen) levels in the buffer solutions, human blood plasma and serum, it has been analyzed some techniques for protein immobilization from immune pair (antibody, antigen) onto the gold surface, as well as for increasing of sensor device («Plasmon» series instrument) sensitivity, stability and productivity, which based on surface plasmon angular spectroscopy with prism excitation.Immobilization of the EBV proteins was performed within the protective-orienting coating — three-dimensional structure of the polysaccharide dextran hydrogel deposited onto the gold film surface on glass substrate, the remaining free sites were blocked with bovine serum albumin molecules (1% BSA) to prevent non-specific interaction, and test the human blood serum from patient with EBV infection under 1:100 delusion in citrate buffer. For oriented attaching of monoclonal antibodies of mouse against the human myoglobin on the surface of gold protein A Staphylococcus aureus conjugate was used, and carry out investigation in human blood serum without delusion. The procedure of formation of am inorganic buffer layer on the surface of a chip for the site-oriented immobilization of biological receptors with the use of the step-like self-organization of the complex ferrocyanides of transition metals (five-ply film of copper aminopentacyanoferrate Cu3 [Fe(CN)5NH3]2) is developed. This gives possibility to get the concentration and calibrating curves (maximum sensitivity of 0,5 μg/ml) for the model fibrinogen in phosphate buffer, and to determine the concentration of fibrinogen in donors blood plasma under 1:300.A four-channel modification of the «Plasmon» series device with successive inquiry of photodiodes massif and with application of comparison reference channel to minimize the effect of the temperature change induced noise has been constructed for the laboratory studies execution. For the large-scale testing of the population, we propose a construction of the sensor in disk format on the basis of the prism scheme of excitation of SPR in the Kretschmann configuration with optical scanning over angles, where a planar chip with integrated gratings for the input-output of light was used as a sensor unit. Fig.: 14. Refs: 45 titles. Роботу присвячено дослідженню фізичних і біохімічних аспектів розробки багатоканальних імуносенсорних приладів з призмовим типом збудження (конфігурація Кречмана) поверхневого плазмонного резо-нансу (ППР) у плівці золота та механічному скануванні кута падіння монохроматичного світла для тестування населення на наявність вірусних і серцево-судинних захворювань при застосуванні специфічної молекулярної реакції антитіло-антиген. Розглянуто схеми іммобілізації білків на золотій поверхні сенсора для швидкого та достовірного виявлення рівня міоглобіну, фібриногену та антитіл до вірусу Епш-тейна-Барр у буферному розчині, сироватці та плазмі крові. Інститут проблем реєстрації інформації НАН України 2020-10-27 Article Article application/pdf http://drsp.ipri.kiev.ua/article/view/218824 10.35681/1560-9189.2020.22.3.218824 Data Recording, Storage & Processing; Vol. 22 No. 3 (2020); 14-30 Регистрация, хранение и обработка данных; Том 22 № 3 (2020); 14-30 Реєстрація, зберігання і обробка даних; Том 22 № 3 (2020); 14-30 1560-9189 uk http://drsp.ipri.kiev.ua/article/view/218824/220149 Авторське право (c) 2021 Реєстрація, зберігання і обробка даних |
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Data Recording, Storage & Processing |
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OJS |
| language |
Ukrainian |
| topic |
поверхневий плазмонний резонанс антитіла міоглобін фібриноген віруси буферні розчини плазма та сироватка крові surface plasmon resonance antibodies viruses myoglobin fibrinogen buffer solutions blood plasma and serum |
| spellingShingle |
поверхневий плазмонний резонанс антитіла міоглобін фібриноген віруси буферні розчини плазма та сироватка крові surface plasmon resonance antibodies viruses myoglobin fibrinogen buffer solutions blood plasma and serum Kostyukevych, K. V. Khrystosenko, R. V. Zagorodnya, S. D. Kostyukevych, S. O. Koptiukh, A. A. Kryuchyn, A. A. Oleksenko, P. F. Molecular diagnostics based on surface plasmon angular spectroscopy |
| topic_facet |
поверхневий плазмонний резонанс антитіла міоглобін фібриноген віруси буферні розчини плазма та сироватка крові surface plasmon resonance antibodies viruses myoglobin fibrinogen buffer solutions blood plasma and serum |
| format |
Article |
| author |
Kostyukevych, K. V. Khrystosenko, R. V. Zagorodnya, S. D. Kostyukevych, S. O. Koptiukh, A. A. Kryuchyn, A. A. Oleksenko, P. F. |
| author_facet |
Kostyukevych, K. V. Khrystosenko, R. V. Zagorodnya, S. D. Kostyukevych, S. O. Koptiukh, A. A. Kryuchyn, A. A. Oleksenko, P. F. |
| author_sort |
Kostyukevych, K. V. |
| title |
Molecular diagnostics based on surface plasmon angular spectroscopy |
| title_short |
Molecular diagnostics based on surface plasmon angular spectroscopy |
| title_full |
Molecular diagnostics based on surface plasmon angular spectroscopy |
| title_fullStr |
Molecular diagnostics based on surface plasmon angular spectroscopy |
| title_full_unstemmed |
Molecular diagnostics based on surface plasmon angular spectroscopy |
| title_sort |
molecular diagnostics based on surface plasmon angular spectroscopy |
| title_alt |
Молекулярна діагностика на основі кутової спектроскопії поверхневих плазмонів |
| description |
With the purpose to reliably detect specific antibodies against the Epstein–Barr virus (EBV) and cardiovascular system molecular markers (myoglobin and fibrinogen) levels in the buffer solutions, human blood plasma and serum, it has been analyzed some techniques for protein immobilization from immune pair (antibody, antigen) onto the gold surface, as well as for increasing of sensor device («Plasmon» series instrument) sensitivity, stability and productivity, which based on surface plasmon angular spectroscopy with prism excitation.Immobilization of the EBV proteins was performed within the protective-orienting coating — three-dimensional structure of the polysaccharide dextran hydrogel deposited onto the gold film surface on glass substrate, the remaining free sites were blocked with bovine serum albumin molecules (1% BSA) to prevent non-specific interaction, and test the human blood serum from patient with EBV infection under 1:100 delusion in citrate buffer. For oriented attaching of monoclonal antibodies of mouse against the human myoglobin on the surface of gold protein A Staphylococcus aureus conjugate was used, and carry out investigation in human blood serum without delusion. The procedure of formation of am inorganic buffer layer on the surface of a chip for the site-oriented immobilization of biological receptors with the use of the step-like self-organization of the complex ferrocyanides of transition metals (five-ply film of copper aminopentacyanoferrate Cu3 [Fe(CN)5NH3]2) is developed. This gives possibility to get the concentration and calibrating curves (maximum sensitivity of 0,5 μg/ml) for the model fibrinogen in phosphate buffer, and to determine the concentration of fibrinogen in donors blood plasma under 1:300.A four-channel modification of the «Plasmon» series device with successive inquiry of photodiodes massif and with application of comparison reference channel to minimize the effect of the temperature change induced noise has been constructed for the laboratory studies execution. For the large-scale testing of the population, we propose a construction of the sensor in disk format on the basis of the prism scheme of excitation of SPR in the Kretschmann configuration with optical scanning over angles, where a planar chip with integrated gratings for the input-output of light was used as a sensor unit. Fig.: 14. Refs: 45 titles. |
| publisher |
Інститут проблем реєстрації інформації НАН України |
| publishDate |
2020 |
| url |
http://drsp.ipri.kiev.ua/article/view/218824 |
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2024-04-21T19:34:16Z |
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2024-04-21T19:34:16Z |
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