A biosensor approach to probe the structure and function of the adsorbed proteins: fibrinogen at the gold surface

• Дата: 1998
• Автори: Snopok, B.A., Kostyukevych, K.V., Rengevych, O.V., Shirshov, Yu.M., Venger, E.F., Kolesnikova, I.N., Lugovskoi, E.V.
• Формат: Стаття
• Мова: English
• Опубліковано: Інститут фізики напівпровідників імені В.Є. Лашкарьова НАН України 1998
• Назва видання: Semiconductor Physics Quantum Electronics & Optoelectronics
• Онлайн доступ: http://dspace.nbuv.gov.ua/handle/123456789/114681
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: A biosensor approach to probe the structure and function of the adsorbed proteins: fibrinogen at the gold surface / B.A. Snopok, K.V. Kostyukevych, O.V. Rengevych, Yu.M. Shirshov, E.F. Venger, I.N. Kolesnikova, E.V. Lugovskoi // Semiconductor Physics Quantum Electronics & Optoelectronics. — 1998. — Т. 1, № 1. — С. 121-134. — Бібліогр.: 50 назв. — англ.

The kinetics of adsorption and surface structure of adsorbed layers of the human fibrinogen on the gold surface, determined by Surface Plasmon Resonance (SPR) and Atomic Force Microscopy (AFM) analysis, was employed to probe the lateral distribution and preferred orientation of protein molecules within the monolayer. In this study, special sets of immunoassays are presented for fibrinogen adsorption/conformation analysis. The results show that kinetic parameters of antigen-antibody interactions are directly related to the interfacial conformation of fibrinogen molecules. Various interfacial structures of adsorbed fibrinogen aggregates, namely single, bi- and three- molecular aggregates, were obtained using a combination of AFM imaging and SPR analysis. Adsorption of fibrinogen onto the surface of polycrystalline gold is a complex process including surface-induced unfolding, local self-assembly and adsorption, occurring concurrently with – and on the time scale of – each other. This result confirmed the utility of the proposed approach for detecting the spatial distribution and biofunctional properties of specific proteins adsorbed from biological liquids in biosensors.