Enhanced cytotoxicity of рhotoexcited fullerene C60 and cisplatin combination against drug-resistant leukemic cells

Enhanced cytotoxicity of рhotoexcited fullerene C60 and cisplatin combination against drug-resistant leukemic cells

Aim: To evaluate the viability of leukemic cells sensitive (L1210S) and resistant (L1210R) to cisplatin, ROS production and free cytosolic Ca²⁺ concentration under treatment with cisplatin or its combination with photoexcited fullerene C₆₀. Methods: Cell viability was assessed by the MTT reduction a...

Повний опис

Збережено в:
Бібліографічні деталі
Дата:2015
Автори: Franskevych, D.V., Prylutska, S.V., Grynyuk, I.I., Grebinyk, D.M., Matyshevska, O.P.
Формат: Стаття
Мова:English
Опубліковано: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2015
Назва видання:Experimental Oncology
Теми:
Original contributions
Онлайн доступ:http://dspace.nbuv.gov.ua/handle/123456789/145486
Теги: Додати тег
Немає тегів, Будьте першим, хто поставить тег для цього запису!
Назва журналу:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Цитувати:Enhanced cytotoxicity of рhotoexcited fullerene C60 and cisplatin combination against drug-resistant leukemic cells / D.V. Franskevych, S.V. Prylutska, I.I. Grynyuk, D.M. Grebinyk, O.P. Matyshevska // Experimental Oncology. — 2015. — Т. 37, № 3. — С. 187-191. — Бібліогр.: 34 назв. — англ.

Репозитарії

Digital Library of Periodicals of National Academy of Sciences of Ukraine
id irk-123456789-145486
record_format dspace
spelling irk-123456789-1454862019-01-24T01:23:23Z Enhanced cytotoxicity of рhotoexcited fullerene C60 and cisplatin combination against drug-resistant leukemic cells Franskevych, D.V. Prylutska, S.V. Grynyuk, I.I. Grebinyk, D.M. Matyshevska, O.P. Original contributions Aim: To evaluate the viability of leukemic cells sensitive (L1210S) and resistant (L1210R) to cisplatin, ROS production and free cytosolic Ca²⁺ concentration under treatment with cisplatin or its combination with photoexcited fullerene C₆₀. Methods: Cell viability was assessed by the MTT reduction assay. Light-emitting diode lamp (2.45 J/сm2) was used for photoexcitation of intracellular accumulated fullerene C₆₀. Free cytosolic calcium concentration ([Ca²⁺]i) and ROS production in cells were estimated with the use of fluorescent probes Indo-1 and 2′,7′-dichlorodihydrofluorescein diacetate (DCF-DA), respectively. Results: It is shown that viability of L1210R cells wasn’t changed under treatment with cisplatin in concentration range 0.1–10 μg/ml. 50% and 30% decrease of L1210S cells were observed after 24 h of incubation with cisplatin at concentrations 5 and 1 μg/ml, respectively. Intensification of extranuclear cytotoxic effects (ROS production and [Ca²⁺]iincrease) after treatment with 1 μg/ml was detected in L1210S, but not in L1210R cells. The most strongly pronounced increase of ROS production and [Ca²⁺]i in both L1210 cell lines was revealed in dynamics after combined treatment with cisplatin (1 μg/ml) and photoexcited fullerene C₆₀ (10⁻⁵ M) and was followed by decreased viability of not only L1210S, but of L1210R cells as well.. Conclusion: Combined treatment with photoexcited C₆₀ and cisplatin allowed to decrease effective concentration of cisplatin against parental L1210 cells and to increase sensibility of resistant cells to the drug. Key Words: cisplatin, L1210 cells, photoexcited fullerene C₆₀, ROS, cytosolic Ca²⁺, cell viability. 2015 Article Enhanced cytotoxicity of рhotoexcited fullerene C60 and cisplatin combination against drug-resistant leukemic cells / D.V. Franskevych, S.V. Prylutska, I.I. Grynyuk, D.M. Grebinyk, O.P. Matyshevska // Experimental Oncology. — 2015. — Т. 37, № 3. — С. 187-191. — Бібліогр.: 34 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/145486 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
language English
topic Original contributions
Original contributions
spellingShingle Original contributions
Original contributions
Franskevych, D.V.
Prylutska, S.V.
Grynyuk, I.I.
Grebinyk, D.M.
Matyshevska, O.P.
Enhanced cytotoxicity of рhotoexcited fullerene C60 and cisplatin combination against drug-resistant leukemic cells
Experimental Oncology
description Aim: To evaluate the viability of leukemic cells sensitive (L1210S) and resistant (L1210R) to cisplatin, ROS production and free cytosolic Ca²⁺ concentration under treatment with cisplatin or its combination with photoexcited fullerene C₆₀. Methods: Cell viability was assessed by the MTT reduction assay. Light-emitting diode lamp (2.45 J/сm2) was used for photoexcitation of intracellular accumulated fullerene C₆₀. Free cytosolic calcium concentration ([Ca²⁺]i) and ROS production in cells were estimated with the use of fluorescent probes Indo-1 and 2′,7′-dichlorodihydrofluorescein diacetate (DCF-DA), respectively. Results: It is shown that viability of L1210R cells wasn’t changed under treatment with cisplatin in concentration range 0.1–10 μg/ml. 50% and 30% decrease of L1210S cells were observed after 24 h of incubation with cisplatin at concentrations 5 and 1 μg/ml, respectively. Intensification of extranuclear cytotoxic effects (ROS production and [Ca²⁺]iincrease) after treatment with 1 μg/ml was detected in L1210S, but not in L1210R cells. The most strongly pronounced increase of ROS production and [Ca²⁺]i in both L1210 cell lines was revealed in dynamics after combined treatment with cisplatin (1 μg/ml) and photoexcited fullerene C₆₀ (10⁻⁵ M) and was followed by decreased viability of not only L1210S, but of L1210R cells as well.. Conclusion: Combined treatment with photoexcited C₆₀ and cisplatin allowed to decrease effective concentration of cisplatin against parental L1210 cells and to increase sensibility of resistant cells to the drug. Key Words: cisplatin, L1210 cells, photoexcited fullerene C₆₀, ROS, cytosolic Ca²⁺, cell viability.
format Article
author Franskevych, D.V.
Prylutska, S.V.
Grynyuk, I.I.
Grebinyk, D.M.
Matyshevska, O.P.
author_facet Franskevych, D.V.
Prylutska, S.V.
Grynyuk, I.I.
Grebinyk, D.M.
Matyshevska, O.P.
author_sort Franskevych, D.V.
title Enhanced cytotoxicity of рhotoexcited fullerene C60 and cisplatin combination against drug-resistant leukemic cells
title_short Enhanced cytotoxicity of рhotoexcited fullerene C60 and cisplatin combination against drug-resistant leukemic cells
title_full Enhanced cytotoxicity of рhotoexcited fullerene C60 and cisplatin combination against drug-resistant leukemic cells
title_fullStr Enhanced cytotoxicity of рhotoexcited fullerene C60 and cisplatin combination against drug-resistant leukemic cells
title_full_unstemmed Enhanced cytotoxicity of рhotoexcited fullerene C60 and cisplatin combination against drug-resistant leukemic cells
title_sort enhanced cytotoxicity of рhotoexcited fullerene c60 and cisplatin combination against drug-resistant leukemic cells
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
publishDate 2015
topic_facet Original contributions
url http://dspace.nbuv.gov.ua/handle/123456789/145486
citation_txt Enhanced cytotoxicity of рhotoexcited fullerene C60 and cisplatin combination against drug-resistant leukemic cells / D.V. Franskevych, S.V. Prylutska, I.I. Grynyuk, D.M. Grebinyk, O.P. Matyshevska // Experimental Oncology. — 2015. — Т. 37, № 3. — С. 187-191. — Бібліогр.: 34 назв. — англ.
series Experimental Oncology
work_keys_str_mv AT franskevychdv enhancedcytotoxicityofrhotoexcitedfullerenec60andcisplatincombinationagainstdrugresistantleukemiccells
AT prylutskasv enhancedcytotoxicityofrhotoexcitedfullerenec60andcisplatincombinationagainstdrugresistantleukemiccells
AT grynyukii enhancedcytotoxicityofrhotoexcitedfullerenec60andcisplatincombinationagainstdrugresistantleukemiccells
AT grebinykdm enhancedcytotoxicityofrhotoexcitedfullerenec60andcisplatincombinationagainstdrugresistantleukemiccells
AT matyshevskaop enhancedcytotoxicityofrhotoexcitedfullerenec60andcisplatincombinationagainstdrugresistantleukemiccells
first_indexed 2023-05-20T17:22:17Z
last_indexed 2023-05-20T17:22:17Z
_version_ 1796153143006855168

Схожі ресурси