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Generation and characterization of polyclonal antibodies specific to N-terminal extension of p85 isoform of ribosomal protein S6 kinase 1 (p85 S6K1)

Aim. Generation of polyclonal antibodies specific to the ribosomal protein S6 kinase isoform – p85S6K1 and directed to the N-terminal (1–23 aa) extension of p85S6K1. Methods. Animal immunization with synthetic (1–23 aa) peptide, ELISA, Western blot, Immunoprecipitation, immunofluorescent analysis. R...

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Bibliographic Details
Main Authors: Savinska, L.O., Klipa, O.M., Khoruzenko, A.I., Shkarina, K.A., Garifulin, O.M., Filonenko, V.V.
Format: Article
Language:English
Published: Інститут молекулярної біології і генетики НАН України 2015
Series:Вiopolymers and Cell
Subjects:
Online Access:http://dspace.nbuv.gov.ua/handle/123456789/152562
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