Generation and characterization of polyclonal antibodies specific to N-terminal extension of p85 isoform of ribosomal protein S6 kinase 1 (p85 S6K1)

• Дата: 2015
• Автори: Savinska, L.O., Klipa, O.M., Khoruzenko, A.I., Shkarina, K.A., Garifulin, O.M., Filonenko, V.V.
• Формат: Стаття
• Мова: English
• Опубліковано: Інститут молекулярної біології і генетики НАН України 2015
• Назва видання: Вiopolymers and Cell
• Теми: Molecular and Cell Biotechnologies
• Онлайн доступ: http://dspace.nbuv.gov.ua/handle/123456789/152562
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: Generation and characterization of polyclonal antibodies specific to N-terminal extension of p85 isoform of ribosomal protein S6 kinase 1 (p85 S6K1) / L.O. Savinska, O.M. Klipa, A.I. Khoruzenko, K.A. Shkarina, O.M. Garifulin, V.V. Filonenko // Вiopolymers and Cell. — 2015. — Т. 31, № 4. — С. 294-300. — Бібліогр.: 20 назв. — англ.

Aim. Generation of polyclonal antibodies specific to the ribosomal protein S6 kinase isoform – p85S6K1 and directed to the N-terminal (1–23 aa) extension of p85S6K1. Methods. Animal immunization with synthetic (1–23 aa) peptide, ELISA, Western blot, Immunoprecipitation, immunofluorescent analysis. Results. Polyclonal antibodies have been generated, which specifically recognize only p85 but not p70 isoform of S6K1 in western blot, immunoprecipitation and immunofluorescence analysis. Conclusions. The obtained antibodies can be recommended for studies on the p85S6K1 and other S6K1 isoforms possessing the N-terminal extension – the identification of binding protein partners, analysis of subcellular localization under different physiological conditions, elucidation of the signal transduction pathways involving different S6K1 isoforms.