2025-02-22T09:59:59-05:00 DEBUG: VuFindSearch\Backend\Solr\Connector: Query fl=%2A&wt=json&json.nl=arrarr&q=id%3A%22irk-123456789-152987%22&qt=morelikethis&rows=5
2025-02-22T09:59:59-05:00 DEBUG: VuFindSearch\Backend\Solr\Connector: => GET http://localhost:8983/solr/biblio/select?fl=%2A&wt=json&json.nl=arrarr&q=id%3A%22irk-123456789-152987%22&qt=morelikethis&rows=5
2025-02-22T09:59:59-05:00 DEBUG: VuFindSearch\Backend\Solr\Connector: <= 200 OK
2025-02-22T09:59:59-05:00 DEBUG: Deserialized SOLR response
Generation of HEK-293 stable cell lines with disrupted expression of ribosomal protein S6 kinase (S6K1) isoforms using the CRISPR/Cas9 genome editing system

Generation of HEK-293 stable cell lines with disrupted expression of ribosomal protein S6 kinase (S6K1) isoforms using the CRISPR/Cas9 genome editing system

Aim. To generate HEK-293 cells with disrupted expression of S6K1 isoforms: p85, p70 and p60. Methods. CRISPR/Cas9 gene editing, Western blotting, immunofluorescent analysis, RT-PCR analysis, MTT assay, scratch assay. Results. Several clones of HEK-293 cells with a complete loss of p85/p70/p60-S6K1 p...

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Bibliographic Details
Main Authors: Zaiets, I.V., Sivchenko, A.S., Khoruzhenko, A.I., Filonenko, V.V.
Format: Article
Language:English
Published: Інститут молекулярної біології і генетики НАН України 2017
Series:Вiopolymers and Cell
Subjects:
Molecular and Cell Biotechnologies
Online Access:http://dspace.nbuv.gov.ua/handle/123456789/152987
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http://dspace.nbuv.gov.ua/handle/123456789/152987

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