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Implementation of the quantitative Real-Time PCR for the molecular-genetic diagnostics of spinal muscular atrophy

Aim. To develop an easy and reliable assay for quantitative analysis of the SMN1 gene exon 7 copy number with Real-Time PCR and a SYBR Green dye which can be used as a test-system for spinal muscular atrophy (SMA) diagnostics. Methods. For the quantification the SMN1 gene exon 7 copies we have used...

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Bibliographic Details
Main Authors: Soloviov, O.O., Livshits, G.B., Podlesnaya, S.S., Livshits, L.A.
Format: Article
Language:English
Published: Інститут молекулярної біології і генетики НАН України 2010
Series:Вiopolymers and Cell
Subjects:
Online Access:http://dspace.nbuv.gov.ua/handle/123456789/153859
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