Fluorescence tools for studies of membrane protein insertion

• Дата: 2018
• Автори: Kyrychenko, A.V., Ladokhin, A.S.
• Формат: Стаття
• Мова: English
• Опубліковано: 2018
• Назва видання: Інститут молекулярної біології і генетики НАН України
• Теми: Reviews
• Онлайн доступ: http://dspace.nbuv.gov.ua/handle/123456789/154274
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: Fluorescence tools for studies of membrane protein insertion / A.V. Kyrychenko, A.S. Ladokhin // Вiopolymers and Cell. — 2018. — Т. 34, № 4. — С. 251-270. — Бібліогр.: 71 назв. — англ.

The transition of soluble proteins into lipid membranes and their refolding therein is fundamental to numerous physiological and disease processes. In this review, we present the summary of the application of fluorescence spectroscopy for studying posttranslational insertion of membrane proteins into lipid bilayers. Various methods utilizing environment-sensitive probes, FRET, FCS and fluorescent quenching for structural and kinetic characterization of protein-lipid interactions are discussed. We also describe the application of steady-state and time-resolved fluorescence quenching by lipid-attached quenchers to characterize the membrane protein immersion into the lipid bilayer. Finally, we illustrate the use of the entire battery of spectroscopic approaches to characterize structural, kinetic and thermodynamic properties of the pH-triggered insertion/refolding pathway of the diphtheria toxin translocation domain.