Preparation of the fluorescein reagent for solid-phase oligonucleotide 5'-labelling and its use for the synthesis of fluorescently labelled PCR primers for HIV-1 detection

• Дата: 1995
• Автори: Dubey, I.Ya., Fedoryak, O.D., Tkachak, O.M., Fedoryak, D.M.
• Формат: Стаття
• Мова: English
• Опубліковано: Інститут молекулярної біології і генетики НАН України 1995
• Назва видання: Биополимеры и клетка
• Онлайн доступ: http://dspace.nbuv.gov.ua/handle/123456789/155758
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: Preparation of the fluorescein reagent for solid-phase oligonucleotide 5'-labelling and its use for the synthesis of fluorescently labelled PCR primers for HIV-1 detection / I.Ya. Dubey, O.D. Fedoryak, O.M. Tkachuk, D.M. Fedoryak // Биополимеры и клетка. — 1995. — Т. 11, № 3-4. — С. 35-41. — Бібліогр.: 19 назв. — англ.

The preparative synthesis of fluorescein H-phosphonate reagent for solid phase 'labelling of oligonucteotides is described. Fluorescein-labelted thymidine and two PCR primers for HIV-1 detection were synthesized using this reagent.