Preparation of the fluorescein reagent for solid-phase oligonucleotide 5'-labelling and its use for the synthesis of fluorescently labelled PCR primers for HIV-1 detection
The preparative synthesis of fluorescein H-phosphonate reagent for solid phase 'labelling of oligonucteotides is described. Fluorescein-labelted thymidine and two PCR primers for HIV-1 detection were synthesized using this reagent.
Збережено в:
| Дата: | 1995 |
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| Автори: | , , , |
| Формат: | Стаття |
| Мова: | English |
| Опубліковано: |
Інститут молекулярної біології і генетики НАН України
1995
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| Назва видання: | Биополимеры и клетка |
| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/155758 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Preparation of the fluorescein reagent for solid-phase oligonucleotide 5'-labelling and its use for the synthesis of fluorescently labelled PCR primers for HIV-1 detection / I.Ya. Dubey, O.D. Fedoryak, O.M. Tkachuk, D.M. Fedoryak // Биополимеры и клетка. — 1995. — Т. 11, № 3-4. — С. 35-41. — Бібліогр.: 19 назв. — англ. |
Репозитарії
Digital Library of Periodicals of National Academy of Sciences of Ukraine| id |
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irk-123456789-1557582019-06-18T01:26:51Z Preparation of the fluorescein reagent for solid-phase oligonucleotide 5'-labelling and its use for the synthesis of fluorescently labelled PCR primers for HIV-1 detection Dubey, I.Ya. Fedoryak, O.D. Tkachak, O.M. Fedoryak, D.M. The preparative synthesis of fluorescein H-phosphonate reagent for solid phase 'labelling of oligonucteotides is described. Fluorescein-labelted thymidine and two PCR primers for HIV-1 detection were synthesized using this reagent. Описано препаративний метод синтезу Н-фосфонатного реагента для флюоресцентного мічення олігонуклеотидів безпосередньо в процесі їх твердофазного синтезу. За допомогою цього реагента синтезовано флюоресцеїн-мічені тимідин та два праймери для ампліфікації дліяики гена HIV-1. Описан препаративный метод синтеза Н-фосфонатного реагента для флюоресцентного мечения олигонуклеотидов непосредственно в процессе пх твердофазного сшпеза. С помощью этого реагента синтезированы флюоресценнмеченные тимидны и два праймера для амплификации участка гена HlV-1. 1995 Article Preparation of the fluorescein reagent for solid-phase oligonucleotide 5'-labelling and its use for the synthesis of fluorescently labelled PCR primers for HIV-1 detection / I.Ya. Dubey, O.D. Fedoryak, O.M. Tkachuk, D.M. Fedoryak // Биополимеры и клетка. — 1995. — Т. 11, № 3-4. — С. 35-41. — Бібліогр.: 19 назв. — англ. 0233-7657 DOI: http://dx.doi.org/10.7124/bc.0003EB http://dspace.nbuv.gov.ua/handle/123456789/155758 577.113.6:542.95 en Биополимеры и клетка Інститут молекулярної біології і генетики НАН України |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| language |
English |
| description |
The preparative synthesis of fluorescein H-phosphonate reagent for solid phase 'labelling of oligonucteotides is described. Fluorescein-labelted thymidine and two PCR primers for HIV-1 detection were synthesized using this reagent. |
| format |
Article |
| author |
Dubey, I.Ya. Fedoryak, O.D. Tkachak, O.M. Fedoryak, D.M. |
| spellingShingle |
Dubey, I.Ya. Fedoryak, O.D. Tkachak, O.M. Fedoryak, D.M. Preparation of the fluorescein reagent for solid-phase oligonucleotide 5'-labelling and its use for the synthesis of fluorescently labelled PCR primers for HIV-1 detection Биополимеры и клетка |
| author_facet |
Dubey, I.Ya. Fedoryak, O.D. Tkachak, O.M. Fedoryak, D.M. |
| author_sort |
Dubey, I.Ya. |
| title |
Preparation of the fluorescein reagent for solid-phase oligonucleotide 5'-labelling and its use for the synthesis of fluorescently labelled PCR primers for HIV-1 detection |
| title_short |
Preparation of the fluorescein reagent for solid-phase oligonucleotide 5'-labelling and its use for the synthesis of fluorescently labelled PCR primers for HIV-1 detection |
| title_full |
Preparation of the fluorescein reagent for solid-phase oligonucleotide 5'-labelling and its use for the synthesis of fluorescently labelled PCR primers for HIV-1 detection |
| title_fullStr |
Preparation of the fluorescein reagent for solid-phase oligonucleotide 5'-labelling and its use for the synthesis of fluorescently labelled PCR primers for HIV-1 detection |
| title_full_unstemmed |
Preparation of the fluorescein reagent for solid-phase oligonucleotide 5'-labelling and its use for the synthesis of fluorescently labelled PCR primers for HIV-1 detection |
| title_sort |
preparation of the fluorescein reagent for solid-phase oligonucleotide 5'-labelling and its use for the synthesis of fluorescently labelled pcr primers for hiv-1 detection |
| publisher |
Інститут молекулярної біології і генетики НАН України |
| publishDate |
1995 |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/155758 |
| citation_txt |
Preparation of the fluorescein reagent for solid-phase oligonucleotide 5'-labelling and its use for the synthesis of fluorescently labelled PCR primers for HIV-1 detection / I.Ya. Dubey, O.D. Fedoryak, O.M. Tkachuk, D.M. Fedoryak // Биополимеры и клетка. — 1995. — Т. 11, № 3-4. — С. 35-41. — Бібліогр.: 19 назв. — англ. |
| series |
Биополимеры и клетка |
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