Expression and secretion of human recombinant LIF by genetically modified mammalian cells

• Дата: 2011
• Автори: Rymar, S.Yu., Ruban, T.A., Irodov, D.M., Kordium, V.A.
• Формат: Стаття
• Мова: English
• Опубліковано: Інститут молекулярної біології і генетики НАН України 2011
• Назва видання: Вiopolymers and Cell
• Теми: Molecular and Cell Biotechnologies
• Онлайн доступ: http://dspace.nbuv.gov.ua/handle/123456789/156336
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: Expression and secretion of human recombinant LIF by genetically modified mammalian cells / S.Yu. Rymar, T.A. Ruban, D.M. Irodov, V.A. Kordium // Вiopolymers and Cell. — 2011. — Т. 27, № 1. — С. 53-58. — Бібліогр.: 29 назв. — англ., рос.

Aim. The aim of this work was to express the human LIF gene in mammalian cells and to study the secretion of recombinant LIF into culture medium. Methods. Recombinant LIF was detected by Western blot analysis and immunoprecipitation in culture medium of CHO-K1, L-M (TK– ) (ins+ ), 293Ò cells, tr...