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Expression and secretion of human recombinant LIF by genetically modified mammalian cells

Expression and secretion of human recombinant LIF by genetically modified mammalian cells

Aim. The aim of this work was to express the human LIF gene in mammalian cells and to study the secretion of recombinant LIF into culture medium. Methods. Recombinant LIF was detected by Western blot analysis and immunoprecipitation in culture medium of CHO-K1, L-M (TK– ) (ins+ ), 293Ò cells, tr...

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Bibliographic Details
Main Authors: Rymar, S.Yu., Ruban, T.A., Irodov, D.M., Kordium, V.A.
Format: Article
Language:English
Published: Інститут молекулярної біології і генетики НАН України 2011
Series:Вiopolymers and Cell
Subjects:
Molecular and Cell Biotechnologies
Online Access:http://dspace.nbuv.gov.ua/handle/123456789/156336
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http://dspace.nbuv.gov.ua/handle/123456789/156336

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