Клонування та експресія білків Mycobacterium bovis MPB63 і MPB83 у клітинах Escherichia coli
Gene fragments mpb83 and mpb63 from Mycobacterium bovis BCG were amplified using PCR. Plasmids containing mpb63 and mpb83 fragments have been constructed using the expressi- on vector pET24a (Novagen). Expression of recombinant proteins was obtained in E. coli Rosetta (DE3) сеlls under control of...
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| Date: | 2007 |
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| Main Authors: | , , , , , , |
| Format: | Article |
| Language: | Ukrainian |
| Published: |
Видавничий дім "Академперіодика" НАН України
2007
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| Subjects: | |
| Online Access: | http://dspace.nbuv.gov.ua/handle/123456789/3152 |
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| Journal Title: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Cite this: | Клонування та експресія білків Mycobacterium bovis MPB63 і MPB83 у клітинах Escherichia coli / Т.А. Редчук, О.С. Олійник, А.А. Кабернюк, Д.О. Буркальова, С.І. Романюк, Д.В. Колибо, С.В. Комісаренко // Доп. НАН України. — 2007. — № 9. — С. 161-166. — Бібліогр.: 12 назв. — укр. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| Summary: | Gene fragments mpb83 and mpb63 from Mycobacterium bovis BCG were amplified using PCR.
Plasmids containing mpb63 and mpb83 fragments have been constructed using the expressi-
on vector pET24a (Novagen). Expression of recombinant proteins was obtained in E. coli
Rosetta (DE3) сеlls under control of strong T7 promoter. Lysates were analyzed by immu-
noblotting with anti HisTag conjugate. His-Tag fusion proteins were purified with batch metal
affіnity chromatography under denaturative conditions. Results are expected to lay groundwork
for the further development of improved diagnostic tools or vaccines against human and bovine
tuberculosis. |
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