Influence of two pt(iv) complexes on viability, apoptosis and cell cycle of B16 mouse melanoma tumors

Influence of two pt(iv) complexes on viability, apoptosis and cell cycle of B16 mouse melanoma tumors

Several platinum(IV) complexes are showing considerable promise in initial trials, producing reactive intermediates that then interact with DNA. Aim: To perform in vitro study of two new platinum(IV) complexes cytotoxic effect on B16 mouse melanoma cells. Methods: PtCl₄ (dbtp)₂ and PtCl₂ (6m...

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Бібліографічні деталі
Опубліковано в: :Experimental Oncology
Дата:2009
Автори: Kaznica, A., Drewa, T., Lakomska, I., Ryta-Stamirowska, P., Debski, R., Styczynski, J., Drewa, G., Szłyk, E.
Формат: Стаття
Мова:English
Опубліковано: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2009
Теми:
Original contributions
Онлайн доступ:https://nasplib.isofts.kiev.ua/handle/123456789/135114
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Назва журналу:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Цитувати:Influence of two pt(iv) complexes on viability, apoptosis and cell cycle of B16 mouse melanoma tumors / A. Kaznica, T. Drewa, I. Lakomska, P. Ryta-Stamirowska, R. Debski, J. Styczynski, G. Drewa, E. Szłyk // Experimental Oncology. — 2009. — Т. 31, № 1. — С. 33–36. — Бібліогр.: 19 назв. — англ.

Репозитарії

Digital Library of Periodicals of National Academy of Sciences of Ukraine
id nasplib_isofts_kiev_ua-123456789-135114
record_format dspace
spelling Kaznica, A.
Drewa, T.
Lakomska, I.
Ryta-Stamirowska, P.
Debski, R.
Styczynski, J.
Drewa, G.
Szłyk, E.
2018-06-14T15:40:56Z
2018-06-14T15:40:56Z
2009
Influence of two pt(iv) complexes on viability, apoptosis and cell cycle of B16 mouse melanoma tumors / A. Kaznica, T. Drewa, I. Lakomska, P. Ryta-Stamirowska, R. Debski, J. Styczynski, G. Drewa, E. Szłyk // Experimental Oncology. — 2009. — Т. 31, № 1. — С. 33–36. — Бібліогр.: 19 назв. — англ.
1812-9269
https://nasplib.isofts.kiev.ua/handle/123456789/135114
Several platinum(IV) complexes are showing considerable promise in initial trials, producing reactive intermediates that then interact with DNA. Aim: To perform in vitro study of two new platinum(IV) complexes cytotoxic effect on B16 mouse melanoma cells. Methods: PtCl₄ (dbtp)₂ and PtCl₂ (6mp)₂ complexes were prepared. PtCl₄ (dbtp)₂ was created as modification of PtCl₄ (dmtp) test previously.Apoptosis and necrosis were examined using flow cytometry, upon Annexin V/PI staining. Results: LC₁₀,LC₅₀ andLC₉₀ parameters established for PtCl₄ (dbtp)₂ were as following: 2.6, 17.0, 58.0 μmol/L. However LC₁₀ andLC₅₀ established for PtCl₂ (6mp)₂ were 1.2 and 14.0μmol/l respectively. The both complexes induced apoptosis. PtCl₂ (6mp)₂ induced cell cycle arrest in G0/G1, while PtCl₄ (dbtp)₂ — in S-phase. Conclusions: PtCl₄ (dbtp)₂ appeared to be more cytotoxic against B16 cells than PtCl₂ (6mp)₂ . Apoptosis was the main mechanism of cell loss in cultures incubated with both tested complexes.
Financial support from Nicolaus Copernicus University grant 551/Ch-CM is greatly acknowledged.
en
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
Experimental Oncology
Original contributions
Influence of two pt(iv) complexes on viability, apoptosis and cell cycle of B16 mouse melanoma tumors
Article
published earlier
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
title Influence of two pt(iv) complexes on viability, apoptosis and cell cycle of B16 mouse melanoma tumors
spellingShingle Influence of two pt(iv) complexes on viability, apoptosis and cell cycle of B16 mouse melanoma tumors
Kaznica, A.
Drewa, T.
Lakomska, I.
Ryta-Stamirowska, P.
Debski, R.
Styczynski, J.
Drewa, G.
Szłyk, E.
Original contributions
title_short Influence of two pt(iv) complexes on viability, apoptosis and cell cycle of B16 mouse melanoma tumors
title_full Influence of two pt(iv) complexes on viability, apoptosis and cell cycle of B16 mouse melanoma tumors
title_fullStr Influence of two pt(iv) complexes on viability, apoptosis and cell cycle of B16 mouse melanoma tumors
title_full_unstemmed Influence of two pt(iv) complexes on viability, apoptosis and cell cycle of B16 mouse melanoma tumors
title_sort influence of two pt(iv) complexes on viability, apoptosis and cell cycle of b16 mouse melanoma tumors
author Kaznica, A.
Drewa, T.
Lakomska, I.
Ryta-Stamirowska, P.
Debski, R.
Styczynski, J.
Drewa, G.
Szłyk, E.
author_facet Kaznica, A.
Drewa, T.
Lakomska, I.
Ryta-Stamirowska, P.
Debski, R.
Styczynski, J.
Drewa, G.
Szłyk, E.
topic Original contributions
topic_facet Original contributions
publishDate 2009
language English
container_title Experimental Oncology
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
format Article
description Several platinum(IV) complexes are showing considerable promise in initial trials, producing reactive intermediates that then interact with DNA. Aim: To perform in vitro study of two new platinum(IV) complexes cytotoxic effect on B16 mouse melanoma cells. Methods: PtCl₄ (dbtp)₂ and PtCl₂ (6mp)₂ complexes were prepared. PtCl₄ (dbtp)₂ was created as modification of PtCl₄ (dmtp) test previously.Apoptosis and necrosis were examined using flow cytometry, upon Annexin V/PI staining. Results: LC₁₀,LC₅₀ andLC₉₀ parameters established for PtCl₄ (dbtp)₂ were as following: 2.6, 17.0, 58.0 μmol/L. However LC₁₀ andLC₅₀ established for PtCl₂ (6mp)₂ were 1.2 and 14.0μmol/l respectively. The both complexes induced apoptosis. PtCl₂ (6mp)₂ induced cell cycle arrest in G0/G1, while PtCl₄ (dbtp)₂ — in S-phase. Conclusions: PtCl₄ (dbtp)₂ appeared to be more cytotoxic against B16 cells than PtCl₂ (6mp)₂ . Apoptosis was the main mechanism of cell loss in cultures incubated with both tested complexes.
issn 1812-9269
url https://nasplib.isofts.kiev.ua/handle/123456789/135114
citation_txt Influence of two pt(iv) complexes on viability, apoptosis and cell cycle of B16 mouse melanoma tumors / A. Kaznica, T. Drewa, I. Lakomska, P. Ryta-Stamirowska, R. Debski, J. Styczynski, G. Drewa, E. Szłyk // Experimental Oncology. — 2009. — Т. 31, № 1. — С. 33–36. — Бібліогр.: 19 назв. — англ.
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AT lakomskai influenceoftwoptivcomplexesonviabilityapoptosisandcellcycleofb16mousemelanomatumors
AT rytastamirowskap influenceoftwoptivcomplexesonviabilityapoptosisandcellcycleofb16mousemelanomatumors
AT debskir influenceoftwoptivcomplexesonviabilityapoptosisandcellcycleofb16mousemelanomatumors
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first_indexed 2025-12-07T16:57:25Z
last_indexed 2025-12-07T16:57:25Z
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