Influence of two pt(iv) complexes on viability, apoptosis and cell cycle of B16 mouse melanoma tumors
Several platinum(IV) complexes are showing considerable promise in initial trials, producing reactive intermediates that then interact with DNA.
 Aim: To perform in vitro study of two new platinum(IV) complexes cytotoxic effect on B16 mouse melanoma cells. Methods: PtCl₄
 (dbtp)₂ a...
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| Published in: | Experimental Oncology |
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| Date: | 2009 |
| Main Authors: | , , , , , , , |
| Format: | Article |
| Language: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2009
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| Online Access: | https://nasplib.isofts.kiev.ua/handle/123456789/135114 |
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| Journal Title: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Cite this: | Influence of two pt(iv) complexes on viability, apoptosis and cell cycle of B16 mouse melanoma tumors / A. Kaznica, T. Drewa, I. Lakomska, P. Ryta-Stamirowska, R. Debski, J. Styczynski, G. Drewa, E. Szłyk // Experimental Oncology. — 2009. — Т. 31, № 1. — С. 33–36. — Бібліогр.: 19 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1862706068609564672 |
|---|---|
| author | Kaznica, A. Drewa, T. Lakomska, I. Ryta-Stamirowska, P. Debski, R. Styczynski, J. Drewa, G. Szłyk, E. |
| author_facet | Kaznica, A. Drewa, T. Lakomska, I. Ryta-Stamirowska, P. Debski, R. Styczynski, J. Drewa, G. Szłyk, E. |
| citation_txt | Influence of two pt(iv) complexes on viability, apoptosis and cell cycle of B16 mouse melanoma tumors / A. Kaznica, T. Drewa, I. Lakomska, P. Ryta-Stamirowska, R. Debski, J. Styczynski, G. Drewa, E. Szłyk // Experimental Oncology. — 2009. — Т. 31, № 1. — С. 33–36. — Бібліогр.: 19 назв. — англ. |
| collection | DSpace DC |
| container_title | Experimental Oncology |
| description | Several platinum(IV) complexes are showing considerable promise in initial trials, producing reactive intermediates that then interact with DNA.
Aim: To perform in vitro study of two new platinum(IV) complexes cytotoxic effect on B16 mouse melanoma cells. Methods: PtCl₄
(dbtp)₂ and
PtCl₂
(6mp)₂
complexes were prepared. PtCl₄
(dbtp)₂ was created as modification of PtCl₄
(dmtp) test previously.Apoptosis and necrosis were examined
using flow cytometry, upon Annexin V/PI staining. Results: LC₁₀,LC₅₀ andLC₉₀ parameters established for PtCl₄
(dbtp)₂
were as following:
2.6, 17.0, 58.0 μmol/L. However LC₁₀ andLC₅₀ established for PtCl₂
(6mp)₂
were 1.2 and 14.0μmol/l respectively. The both complexes induced
apoptosis. PtCl₂
(6mp)₂
induced cell cycle arrest in G0/G1, while PtCl₄
(dbtp)₂ — in S-phase. Conclusions: PtCl₄
(dbtp)₂
appeared to be more
cytotoxic against B16 cells than PtCl₂
(6mp)₂
. Apoptosis was the main mechanism of cell loss in cultures incubated with both tested complexes.
|
| first_indexed | 2025-12-07T16:57:25Z |
| format | Article |
| fulltext | |
| id | nasplib_isofts_kiev_ua-123456789-135114 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1812-9269 |
| language | English |
| last_indexed | 2025-12-07T16:57:25Z |
| publishDate | 2009 |
| publisher | Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| record_format | dspace |
| spelling | Kaznica, A. Drewa, T. Lakomska, I. Ryta-Stamirowska, P. Debski, R. Styczynski, J. Drewa, G. Szłyk, E. 2018-06-14T15:40:56Z 2018-06-14T15:40:56Z 2009 Influence of two pt(iv) complexes on viability, apoptosis and cell cycle of B16 mouse melanoma tumors / A. Kaznica, T. Drewa, I. Lakomska, P. Ryta-Stamirowska, R. Debski, J. Styczynski, G. Drewa, E. Szłyk // Experimental Oncology. — 2009. — Т. 31, № 1. — С. 33–36. — Бібліогр.: 19 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/135114 Several platinum(IV) complexes are showing considerable promise in initial trials, producing reactive intermediates that then interact with DNA.
 Aim: To perform in vitro study of two new platinum(IV) complexes cytotoxic effect on B16 mouse melanoma cells. Methods: PtCl₄
 (dbtp)₂ and
 PtCl₂
 (6mp)₂
 complexes were prepared. PtCl₄
 (dbtp)₂ was created as modification of PtCl₄
 (dmtp) test previously.Apoptosis and necrosis were examined
 using flow cytometry, upon Annexin V/PI staining. Results: LC₁₀,LC₅₀ andLC₉₀ parameters established for PtCl₄
 (dbtp)₂
 were as following:
 2.6, 17.0, 58.0 μmol/L. However LC₁₀ andLC₅₀ established for PtCl₂
 (6mp)₂
 were 1.2 and 14.0μmol/l respectively. The both complexes induced
 apoptosis. PtCl₂
 (6mp)₂
 induced cell cycle arrest in G0/G1, while PtCl₄
 (dbtp)₂ — in S-phase. Conclusions: PtCl₄
 (dbtp)₂
 appeared to be more
 cytotoxic against B16 cells than PtCl₂
 (6mp)₂
 . Apoptosis was the main mechanism of cell loss in cultures incubated with both tested complexes. Financial support from Nicolaus Copernicus University
 grant 551/Ch-CM is greatly acknowledged. en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Original contributions Influence of two pt(iv) complexes on viability, apoptosis and cell cycle of B16 mouse melanoma tumors Article published earlier |
| spellingShingle | Influence of two pt(iv) complexes on viability, apoptosis and cell cycle of B16 mouse melanoma tumors Kaznica, A. Drewa, T. Lakomska, I. Ryta-Stamirowska, P. Debski, R. Styczynski, J. Drewa, G. Szłyk, E. Original contributions |
| title | Influence of two pt(iv) complexes on viability, apoptosis and cell cycle of B16 mouse melanoma tumors |
| title_full | Influence of two pt(iv) complexes on viability, apoptosis and cell cycle of B16 mouse melanoma tumors |
| title_fullStr | Influence of two pt(iv) complexes on viability, apoptosis and cell cycle of B16 mouse melanoma tumors |
| title_full_unstemmed | Influence of two pt(iv) complexes on viability, apoptosis and cell cycle of B16 mouse melanoma tumors |
| title_short | Influence of two pt(iv) complexes on viability, apoptosis and cell cycle of B16 mouse melanoma tumors |
| title_sort | influence of two pt(iv) complexes on viability, apoptosis and cell cycle of b16 mouse melanoma tumors |
| topic | Original contributions |
| topic_facet | Original contributions |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/135114 |
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