Stress-Induced Increases in the Levels of Caspases in the Prefrontal Cortex in a Rat Model of PTSD
We investigated the levels of expression of caspase-3 and 9 in the medial prefrontal cortex (mPFC) of rats subjected to single prolonged stress (SPS), trying to provide a novel insight into the mechanism of how this cortical region is related to post-traumatic stress disorder (PTSD). Fifty male W...
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| Date: | 2016 |
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| Language: | English |
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Інститут фізіології ім. О.О. Богомольця НАН України
2016
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| Series: | Нейрофизиология |
| Online Access: | https://nasplib.isofts.kiev.ua/handle/123456789/148322 |
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| Cite this: | Stress-Induced Increases in the Levels of Caspases in the Prefrontal Cortex in a Rat Model of PTSD / J.H. Zhang, M. Li, F. Han, Yu.X. Shi // Нейрофизиология. — 2016. — Т. 48, № 1. — С. 14-19. — Бібліогр.: 23 назв. — англ. |
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nasplib_isofts_kiev_ua-123456789-1483222025-02-09T10:50:46Z Stress-Induced Increases in the Levels of Caspases in the Prefrontal Cortex in a Rat Model of PTSD Zhang, J.H. Li, M. Han, F. Yu X. Shi We investigated the levels of expression of caspase-3 and 9 in the medial prefrontal cortex (mPFC) of rats subjected to single prolonged stress (SPS), trying to provide a novel insight into the mechanism of how this cortical region is related to post-traumatic stress disorder (PTSD). Fifty male Wistar rats were divided into the control group and four SPS groups examined at days 1,4,7, and 14 after treatment. Expression of caspase-3 in SPS groups was significantly greater when compared with the control group (P < 0.05) and peaked at day 7 after exposure to SPS. In the control group, the intensity of fluorescence of caspase-9- positive cells was low, while that in the SPS groups was significantly higher (P < 0.01) and peaked at day 4 after exposure to SPS. After SPS episodes, levels of mRNA of caspase-3 and caspase-9, compared with those in the control group, gradually increased and peaked at days 7 and 4, respectively (P < 0.01). Therefore, changes of expression of caspase-9 and caspase-3 may play an important role in the pathogenesis of PTSD. Ми досліджували рівні експресії каспаз-3 та 9 у медіальній префронтальній корі (mPFC) щурів, підданих поодинокому епізоду тривалого стресу (SPS), намагаючись отримати нові дані щодо механізмів залучення цієї ділянки кори в патогенез посттравматичного стресового розладу (PTSD). 50 самців щурів лінії Вістар були поділені на контрольну групу та SPS-групи, досліджені через одну, чотири, сім та 14 діб після дії SPS. Експресія каспази-3 в групах SPS була вірогідно посиленою порівняно з такою в контрольній групі (P < 0.05), досягаючи найвищого рівня через сім діб після впливу SPS. У контрольній групі флуоресценція продуктів виявлення каспази-9 була слабкою, тоді як у групах SPS вона була істотно сильнішою (P < 0.01); її рівень досягав максимуму через чотири доби після дії SPS. Після епізодів SPS рівні мРНК каспаз-3 та 9 поступово збільшувалися, досягаючи пікових значень на сьому та четверту добу відповідно (P < 0.01). Отже, зміни експресії каспази-3 та каспази-9 відіграють істотну роль у патогенезі PTSD. The authors thank all the staff members in the China Medical University Experiment Center for their technical support on how to improve the quality of the paper. This research has been supported by a grant from the National Natural Science Foundation of China (No. 81171282). 2016 Article Stress-Induced Increases in the Levels of Caspases in the Prefrontal Cortex in a Rat Model of PTSD / J.H. Zhang, M. Li, F. Han, Yu.X. Shi // Нейрофизиология. — 2016. — Т. 48, № 1. — С. 14-19. — Бібліогр.: 23 назв. — англ. https://nasplib.isofts.kiev.ua/handle/123456789/148322 612.825:616.009.8 en Нейрофизиология application/pdf Інститут фізіології ім. О.О. Богомольця НАН України |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| language |
English |
| description |
We investigated the levels of expression of caspase-3 and 9 in the medial prefrontal cortex
(mPFC) of rats subjected to single prolonged stress (SPS), trying to provide a novel insight
into the mechanism of how this cortical region is related to post-traumatic stress disorder
(PTSD). Fifty male Wistar rats were divided into the control group and four SPS groups
examined at days 1,4,7, and 14 after treatment. Expression of caspase-3 in SPS groups was
significantly greater when compared with the control group (P < 0.05) and peaked at day
7 after exposure to SPS. In the control group, the intensity of fluorescence of caspase-9-
positive cells was low, while that in the SPS groups was significantly higher (P < 0.01) and
peaked at day 4 after exposure to SPS. After SPS episodes, levels of mRNA of caspase-3 and
caspase-9, compared with those in the control group, gradually increased and peaked at days
7 and 4, respectively (P < 0.01). Therefore, changes of expression of caspase-9 and caspase-3
may play an important role in the pathogenesis of PTSD. |
| format |
Article |
| author |
Zhang, J.H. Li, M. Han, F. Yu X. Shi |
| spellingShingle |
Zhang, J.H. Li, M. Han, F. Yu X. Shi Stress-Induced Increases in the Levels of Caspases in the Prefrontal Cortex in a Rat Model of PTSD Нейрофизиология |
| author_facet |
Zhang, J.H. Li, M. Han, F. Yu X. Shi |
| author_sort |
Zhang, J.H. |
| title |
Stress-Induced Increases in the Levels of Caspases in the Prefrontal Cortex in a Rat Model of PTSD |
| title_short |
Stress-Induced Increases in the Levels of Caspases in the Prefrontal Cortex in a Rat Model of PTSD |
| title_full |
Stress-Induced Increases in the Levels of Caspases in the Prefrontal Cortex in a Rat Model of PTSD |
| title_fullStr |
Stress-Induced Increases in the Levels of Caspases in the Prefrontal Cortex in a Rat Model of PTSD |
| title_full_unstemmed |
Stress-Induced Increases in the Levels of Caspases in the Prefrontal Cortex in a Rat Model of PTSD |
| title_sort |
stress-induced increases in the levels of caspases in the prefrontal cortex in a rat model of ptsd |
| publisher |
Інститут фізіології ім. О.О. Богомольця НАН України |
| publishDate |
2016 |
| url |
https://nasplib.isofts.kiev.ua/handle/123456789/148322 |
| citation_txt |
Stress-Induced Increases in the Levels of Caspases in the Prefrontal Cortex in a Rat Model of PTSD / J.H. Zhang, M. Li, F. Han, Yu.X. Shi // Нейрофизиология. — 2016. — Т. 48, № 1. — С. 14-19. — Бібліогр.: 23 назв. — англ. |
| series |
Нейрофизиология |
| work_keys_str_mv |
AT zhangjh stressinducedincreasesinthelevelsofcaspasesintheprefrontalcortexinaratmodelofptsd AT lim stressinducedincreasesinthelevelsofcaspasesintheprefrontalcortexinaratmodelofptsd AT hanf stressinducedincreasesinthelevelsofcaspasesintheprefrontalcortexinaratmodelofptsd AT yuxshi stressinducedincreasesinthelevelsofcaspasesintheprefrontalcortexinaratmodelofptsd |
| first_indexed |
2025-11-25T20:44:30Z |
| last_indexed |
2025-11-25T20:44:30Z |
| _version_ |
1849796560954589184 |