Bacterial expression and isotope labeling of AIMP1/p43 codosome protein for structural studies by multidimensional NMR spectroscopy

• Published in: Вiopolymers and Cell
• Date: 2015
• Main Authors: Vorobyova, N.V., Lozhko, D.N., Zhukov, I.Yu., Kornelyuk, A.I.
• Format: Article
• Language: English
• Published: Інститут молекулярної біології і генетики НАН України 2015
• Subjects: Structure and Function of Biopolymers
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/152441
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Bacterial expression and isotope labeling of AIMP1/p43 codosome protein for structural studies by multidimensional NMR spectroscopy / N.V. Vorobyova, D.N. Lozhko, I.Yu. Zhukov, A.I. Kornelyuk // Вiopolymers and Cell. — 2015. — Т. 31, № 2. — С. 109-114. — Бібліогр.: 18 назв. — англ.

AIMP1/p43 protein is a structural component of multisynthetase complex (codosome) in eukaryotes, which reveals both tRNA binding and cytokine activities. Aim. Bacterial expression and purification of isotopically-labeled recombinant AIMP1/p43 protein in E. coli cells for studying its solution struct...