Bacterial expression and isotope labeling of AIMP1/p43 codosome protein for structural studies by multidimensional NMR spectroscopy
AIMP1/p43 protein is a structural component of multisynthetase complex (codosome) in eukaryotes, which reveals both tRNA binding and cytokine activities. Aim. Bacterial expression and purification of isotopically-labeled recombinant AIMP1/p43 protein in E. coli cells for studying its solution struct...
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| Published in: | Вiopolymers and Cell |
|---|---|
| Date: | 2015 |
| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
| Published: |
Інститут молекулярної біології і генетики НАН України
2015
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| Subjects: | |
| Online Access: | https://nasplib.isofts.kiev.ua/handle/123456789/152441 |
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| Journal Title: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Cite this: | Bacterial expression and isotope labeling of AIMP1/p43 codosome protein for structural studies by multidimensional NMR spectroscopy / N.V. Vorobyova, D.N. Lozhko, I.Yu. Zhukov, A.I. Kornelyuk // Вiopolymers and Cell. — 2015. — Т. 31, № 2. — С. 109-114. — Бібліогр.: 18 назв. — англ. |