Generation and characterization of polyclonal antibodies specific to N-terminal extension of p85 isoform of ribosomal protein S6 kinase 1 (p85 S6K1)

• Published in: Вiopolymers and Cell
• Date: 2015
• Main Authors: Savinska, L.O., Klipa, O.M., Khoruzenko, A.I., Shkarina, K.A., Garifulin, O.M., Filonenko, V.V.
• Format: Article
• Language: English
• Published: Інститут молекулярної біології і генетики НАН України 2015
• Subjects: Molecular and Cell Biotechnologies
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/152562
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Generation and characterization of polyclonal antibodies specific to N-terminal extension of p85 isoform of ribosomal protein S6 kinase 1 (p85 S6K1) / L.O. Savinska, O.M. Klipa, A.I. Khoruzenko, K.A. Shkarina, O.M. Garifulin, V.V. Filonenko // Вiopolymers and Cell. — 2015. — Т. 31, № 4. — С. 294-300. — Бібліогр.: 20 назв. — англ.

Aim. Generation of polyclonal antibodies specific to the ribosomal protein S6 kinase isoform – p85S6K1 and directed to the N-terminal (1–23 aa) extension of p85S6K1. Methods. Animal immunization with synthetic (1–23 aa) peptide, ELISA, Western blot, Immunoprecipitation, immunofluorescent analysis. R...