Development of the chromatographic medium for the affinity isolation of the recombinant hIFN-β1b based on immobilized single-chain antibodies

• Date: 2015
• Main Authors: Pokholenko, Ia.O., Gorbatiuk, O.B., Okunev, O.V., Irodov, D.M., Degtiarova, M.I., Palivoda, O.G., Kordium, V.A.
• Format: Article
• Language: English
• Published: Інститут молекулярної біології і генетики НАН України 2015
• Series: Вiopolymers and Cell
• Subjects: Molecular and Cell Biotechnologies
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/152564
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Development of the chromatographic medium for the affinity isolation of the recombinant hIFN-β1b based on immobilized single-chain antibodies / Ia.O. Pokholenko, O.B. Gorbatiuk, O.V. Okunev, D.M. Irodov, M.I. Degtiarova, O.G. Palivoda, V.A. Kordium // Вiopolymers and Cell. — 2015. — Т. 31, № 4. — С. 279-284. — Бібліогр.: 14 назв. — англ.

Aim. The development of a laboratory method for the production of immunoaffinity chromatography medium for the purification of the recombinant human IFN-β1b. Methods. A gene of the chimeric protein ScFvbINF-CBD was constructed using the DNA sequences encoding ScFv specific to hIFN-β1b and the cellul...