Study on interactions of human IgG with immobilized anti-IgG or recombinant Staphylococcal protein A using surface plasmon resonance spectrometry

• Published in: Вiopolymers and Cell
• Date: 2016
• Main Authors: Bakhmachuk, A.O., Gorbatiuk, O.B., Palyvoda, O.G., Dons'koi, B.V., Rachkov, A.E., Soldatkin, A.P.
• Format: Article
• Language: English
• Published: Інститут молекулярної біології і генетики НАН України 2016
• Subjects: Molecular and Cell Biotechnologies
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/152768
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Study on interactions of human IgG with immobilized anti-IgG or recombinant Staphylococcal protein A using surface plasmon resonance spectrometry / A.O. Bakhmachuk, O.B. Gorbatiuk, O.G. Palyvoda, B.V. Dons'koi, A.E. Rachkov, A.P. Soldatkin // Вiopolymers and Cell. — 2016. — Т. 32, № 1. — С. 54-60. — Бібліогр.: 14 назв. — англ.

Aim. Comparison of the IgG-binding activity of recombinant Staphylococcal protein A with introduced C-terminal cysteine residue (SPA-Cys) or goat anti-human IgG antibodies (anti-IgG) after their immobilization on a gold sensor surface of surface plasmon resonance (SPR) spectrometer. Methods. SPA-Cys...