Production, purification of the recombinant analog of Y-box-binding protein and its interaction with poly(ADP-ribose), RNA, single- and double-stranded DNAs

• Published in: Вiopolymers and Cell
• Date: 2017
• Main Authors: Alemasova, E.E., Naumenko, K.N., Pestryakov, P.E., Lavrik, O.I.
• Format: Article
• Language: English
• Published: Інститут молекулярної біології і генетики НАН України 2017
• Subjects: Molecular and Cell Biotechnologies
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/152976
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Production, purification of the recombinant analog of Y-box-binding protein and its interaction with poly(ADP-ribose), RNA, single- and double-stranded DNAs / E.E. Alemasova, K.N. Naumenko, P.E. Pestryakov, O.I. Lavrik // Вiopolymers and Cell. — 2017. — Т. 33, № 3. — С. 214-220. — Бібліогр.: 8 назв. — англ.

Aim. Production and purification of the recombinant histidine-tagged Y-box- binding protein and study of its interaction with DNA and poly(ADP-ribose). Methods. Ligation-independent cloning, PCR, Sanger sequencing, protein chromatography, polyacrylamide gel electrophoresis, and electrophoresis mobil...