Generation of HEK-293 stable cell lines with disrupted expression of ribosomal protein S6 kinase (S6K1) isoforms using the CRISPR/Cas9 genome editing system

• Published in: Вiopolymers and Cell
• Date: 2017
• Main Authors: Zaiets, I.V., Sivchenko, A.S., Khoruzhenko, A.I., Filonenko, V.V.
• Format: Article
• Language: English
• Published: Інститут молекулярної біології і генетики НАН України 2017
• Subjects: Molecular and Cell Biotechnologies
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/152987
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Generation of HEK-293 stable cell lines with disrupted expression of ribosomal protein S6 kinase (S6K1) isoforms using the CRISPR/Cas9 genome editing system / I.V. Zaiets, A.S. Sivchenko, A.I. Khoruzhenko, V.V. Filonenko // Вiopolymers and Cell. — 2017. — Т. 33, № 5. — С. 356-366. — Бібліогр.: 39 назв. — англ.

Aim. To generate HEK-293 cells with disrupted expression of S6K1 isoforms: p85, p70 and p60. Methods. CRISPR/Cas9 gene editing, Western blotting, immunofluorescent analysis, RT-PCR analysis, MTT assay, scratch assay. Results. Several clones of HEK-293 cells with a complete loss of p85/p70/p60-S6K1 p...