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Generation of HEK-293 stable cell lines with disrupted expression of ribosomal protein S6 kinase (S6K1) isoforms using the CRISPR/Cas9 genome editing system

Aim. To generate HEK-293 cells with disrupted expression of S6K1 isoforms: p85, p70 and p60. Methods. CRISPR/Cas9 gene editing, Western blotting, immunofluorescent analysis, RT-PCR analysis, MTT assay, scratch assay. Results. Several clones of HEK-293 cells with a complete loss of p85/p70/p60-S6K1 p...

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Veröffentlicht in:	Вiopolymers and Cell
Datum:	2017
Hauptverfasser:	Zaiets, I.V., Sivchenko, A.S., Khoruzhenko, A.I., Filonenko, V.V.
Format:	Artikel
Sprache:	Englisch
Veröffentlicht:	Інститут молекулярної біології і генетики НАН України 2017
Schlagworte:	Molecular and Cell Biotechnologies
Online Zugang:	https://nasplib.isofts.kiev.ua/handle/123456789/152987
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Назва журналу:	Digital Library of Periodicals of National Academy of Sciences of Ukraine
Zitieren:	Generation of HEK-293 stable cell lines with disrupted expression of ribosomal protein S6 kinase (S6K1) isoforms using the CRISPR/Cas9 genome editing system / I.V. Zaiets, A.S. Sivchenko, A.I. Khoruzhenko, V.V. Filonenko // Вiopolymers and Cell. — 2017. — Т. 33, № 5. — С. 356-366. — Бібліогр.: 39 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine

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