Lepidine Orange derivative as a new dye for sensitive fluorescent detection of DNA

• Published in: Вiopolymers and Cell
• Date: 2013
• Main Authors: Didan, Yu.V., Kryvorotenko, D.V., Negrutska, V.V., Dubey, I.Ya.
• Format: Article
• Language: English
• Published: Інститут молекулярної біології і генетики НАН України 2013
• Subjects: Bioorganic Chemistry
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/153676
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Lepidine Orange derivative as a new dye for sensitive fluorescent detection of DNA / Yu.V. Didan, D.V. Kryvorotenko, V.V. Negrutska, I.Ya. Dubey // Вiopolymers and Cell. — 2013. — Т. 29, №. 6. — С. 511-514. — Бібліогр.: 13 назв. — англ.

Aim. To study new cyanine dye LO-7 as a reagent for dsDNA visualization in electrophoretic gels. Methods. Gel electrophoresis, restriction, fluorescence detection, mobility shift assay. Results. LO-7 binds to DNA to form stable highly fluorescent complexes. As little as 80 pg DNA can be detected in...