Comparative analysis of epigenetic markers in plasma and tissue of patients with colorectal cancer

• Date: 2014
• Main Authors: Kondratov, A.G., Nekrasov, K.A., Lototska, L.V., Panasenko, G.V., Stoliar, L.A., Lapska, Y.V., Kolesnyk, O.O., Shchepotin, I.B., Rynditch, A.V., Kashuba, V.I.
• Format: Article
• Language: English
• Published: Інститут молекулярної біології і генетики НАН України 2014
• Series: Вiopolymers and Cell
• Subjects: Biomedicine
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/153751
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Comparative analysis of epigenetic markers in plasma and tissue of patients with colorectal cancer / A.G. Kondratov, K.A. Nekrasov, L.V. Lototska, G.V. Panasenko, L.A. Stoliar, Y.V. Lapska, O.O. Kolesnyk, I.B. Shchepotin, A.V. Rynditch, V.I. Kashuba // Вiopolymers and Cell. — 2014. — Т. 30, № 2. — С. 129-134. — Бібліогр.: 19 назв. — англ.

Aim. The work is devoted to the development of less invasive tools for the colorectal cancer (CRC) screening. Methods. Q-PCR and methylation-specific PCR techniques were used in the current work. Results. We have shown that the levels of cell-free plasma DNA are higher in the CRC patients compared with the healthy donors (p < 0.01). Hypermethylation of APC, FHIT, LRRC3B and HIC1 genes was studied in the tumor and plasma samples of CRC patients. Two-stage verification for CRC screening was proposed. Conclusions. We proposed and tested a novel approach for CRC screening based on the determination of cell-free DNA and methylated DNA fragments in the plasma.