Development of ARMS PCR tests for detection of common CFTR gene mutations

• Published in: Вiopolymers and Cell
• Date: 2010
• Main Authors: Soloviov, O.O., Pampukha, V.M., Livshits, L.A.
• Format: Article
• Language: English
• Published: Інститут молекулярної біології і генетики НАН України 2010
• Subjects: Biomedicine
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/154183
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Development of ARMS PCR tests for detection of common CFTR gene mutations / O.O. Soloviov, V.M. Pampukha, L.A. Livshits // Вiopolymers and Cell. — 2010. — Т. 26, № 5. — С. 378-383. — Бібліогр.: 12 назв. — англ.

Aim. To develop diagnostic assays, based on the amplification refractory mutation system (ARMS) principle, for detection of common mutations in the CFTR gene using two approaches: standard PCR with further gel-electrophoresis and Real-Time PCR with SYBR Green. Materials. For this study we have chose...